CN107208019B - 用于核酸单克隆簇的产生和测序的方法和阵列 - Google Patents

用于核酸单克隆簇的产生和测序的方法和阵列 Download PDF

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CN107208019B
CN107208019B CN201580072860.9A CN201580072860A CN107208019B CN 107208019 B CN107208019 B CN 107208019B CN 201580072860 A CN201580072860 A CN 201580072860A CN 107208019 B CN107208019 B CN 107208019B
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capture
primers
primer
primer pair
capture primer
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CN107208019A (zh
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凯文·L·冈德森
白经纬
马修·威廉·克林格
约翰·M·拜尔勒
乔纳森·马克·鲍特尔
罗伯托·里加蒂
玛丽亚·坎德拉里亚·罗杰特巴奇伽鲁普
博扬·波亚诺夫
克劳斯·梅辛格
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Illumina Cambridge Ltd
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CN201580072860.9A 2014-11-11 2015-11-11 用于核酸单克隆簇的产生和测序的方法和阵列 Active CN107208019B (zh)

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US201462078346P 2014-11-11 2014-11-11
US62/078,346 2014-11-11
US201462096464P 2014-12-23 2014-12-23
US62/096,464 2014-12-23
PCT/EP2015/076353 WO2016075204A1 (en) 2014-11-11 2015-11-11 Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

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Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2697397B1 (en) 2011-04-15 2017-04-05 The Johns Hopkins University Safe sequencing system
WO2014070462A1 (en) 2012-10-29 2014-05-08 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
KR20160039229A (ko) 2013-07-30 2016-04-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 정량적 dna-기반 영상화 및 초해상도 영상화
US10415083B2 (en) 2013-10-28 2019-09-17 The Translational Genomics Research Institute Long insert-based whole genome sequencing
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3283656A4 (en) * 2015-04-17 2018-12-05 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
CN107924473B (zh) * 2015-08-07 2021-11-23 哈佛学院院长及董事 蛋白质-蛋白质相互作用的超分辨成像
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
US20180195115A1 (en) * 2016-12-22 2018-07-12 Illumina, Inc. Arrays with quality control tracers
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
NL2019044B1 (en) * 2017-05-11 2018-11-15 Illumina Inc Protective surface coatings for flow cells
WO2018218136A1 (en) * 2017-05-25 2018-11-29 Integrated Dna Technologies, Inc. Reverse complement adapters for the mitigation of umi hopping
US11001885B2 (en) 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
CN107523640A (zh) * 2017-10-12 2017-12-29 厦门燕旭安生物科技有限公司 一种ctDNA精准测序的扩增子文库构建方法
JP7087010B2 (ja) * 2017-12-21 2022-06-20 イラミーナ インコーポレーテッド ヒドロゲルコーティングを有するフローセル
JP2021525095A (ja) * 2018-05-28 2021-09-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｄｎａ−ポア−ポリメラーゼ複合体の酵素的濃縮
AU2019275675A1 (en) 2018-06-29 2020-01-16 Illumina Cambridge Limited Flow cells
TWI730352B (zh) 2018-08-06 2021-06-11 英商伊路米納劍橋有限公司 流體槽與定序系統及製備用於其之基板的方法
US20200149095A1 (en) * 2018-11-14 2020-05-14 Element Biosciences, Inc. Low binding supports for improved solid-phase dna hybridization and amplification
SG11202012762WA (en) * 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
KR102872035B1 (ko) 2018-12-18 2025-10-16 일루미나 케임브리지 리미티드 단일 표면 프라이머를 사용하는 페어드 엔드 서열분석을 위한 방법 및 조성물
CN112654715B (zh) * 2018-12-19 2024-12-31 亿明达股份有限公司 改进多核苷酸簇克隆性优先性的方法
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
TWI873119B (zh) * 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
WO2020243073A1 (en) * 2019-05-31 2020-12-03 Illumina, Inc. Systems and methods for information storage and retrieval using flow cells
JP7272431B2 (ja) 2019-05-31 2023-05-12 日本電気株式会社 情報処理装置、情報処理方法及び情報処理プログラム
TW202120693A (zh) * 2019-08-21 2021-06-01 美商伊路米納有限公司 偵測核苷酸之聚合酶併入的方法
EP4077726B1 (en) 2019-12-16 2025-04-23 Illumina, Inc. Kits and flow cells
WO2021127357A2 (en) 2019-12-20 2021-06-24 Illumina, Inc. Flow cells
CN114207148B (zh) * 2019-12-23 2025-04-22 伊鲁米纳公司 具有用于模板多核苷酸附接的单个位点的纳米颗粒
WO2021163546A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
WO2021168097A1 (en) * 2020-02-19 2021-08-26 Genapsys, Inc. Methods and systems for nucleic acid processing
BR112021026795A2 (pt) * 2020-02-26 2022-02-15 Illumina Cambridge Ltd Kits para genotipagem
WO2021178467A1 (en) * 2020-03-03 2021-09-10 Omniome, Inc. Methods and compositions for sequencing double stranded nucleic acids
CA3145466A1 (en) * 2020-03-09 2021-09-16 Illumina, Inc. Improvements in nucleic acid sequencing
AU2021271637A1 (en) 2020-05-12 2022-12-08 Illumina Singapore Pte. Ltd. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
AU2021341688B2 (en) 2020-09-14 2025-09-18 Illumina Cambridge Limited Compositions and methods for amplifying polynucleotides
IL299439A (en) * 2020-09-29 2023-02-01 Illumina Inc Flow cell manufacturing methods
CA3183189A1 (en) 2020-09-29 2022-04-07 Illumina, Inc. Flow cells and methods for making the same
CA3183716A1 (en) * 2020-12-10 2022-06-16 Lewis Kraft Image sensor structures and related methods
WO2022192671A1 (en) * 2021-03-12 2022-09-15 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
WO2022229137A1 (en) * 2021-04-30 2022-11-03 Illumina Cambridge Limited Flow cell and methods
US20220356519A1 (en) * 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
WO2024006625A1 (en) * 2022-06-27 2024-01-04 Somalogic Operating Co., Inc. Monoclonal polony generation using nucleic acid supramolecular structures
US20240076722A1 (en) * 2022-06-29 2024-03-07 10X Genomics, Inc. Compositions and methods for oligonucleotide inversion on arrays
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法
WO2024057089A1 (en) * 2022-09-09 2024-03-21 Todx Inc. Device for parallel detection and quantification of nucleic acid based markers
KR20250069478A (ko) * 2022-09-16 2025-05-19 일루미나, 인코포레이티드 폴리뉴클레오티드 결합부위를 갖는 나노입자 및 이의 제조방법
WO2024256580A1 (en) * 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025062001A1 (en) * 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
US20250188529A1 (en) 2023-12-12 2025-06-12 Illumina, Inc. Capturing and amplifying polynucleotides using molecules and particles
US20250207193A1 (en) * 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
CN117778578B (zh) * 2023-12-29 2024-10-15 深圳海普洛斯医学检验实验室 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP3673986A1 (en) 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
AU2005296200B2 (en) 2004-09-17 2011-07-14 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
US7682816B2 (en) 2005-04-07 2010-03-23 454 Life Sciences Corporation Thin film coated microwell arrays and methods of using same
EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP5122555B2 (ja) 2006-03-31 2013-01-16 ソレクサ・インコーポレイテッド 合成解析による配列決定システムおよび装置
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP4134667B1 (en) 2006-12-14 2025-11-12 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
SI2669387T1 (sl) 2009-08-25 2017-01-31 Illumina, Inc. Metode za izbiro in pomnoževanje polinukleotidov
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
DK2768972T3 (en) 2011-09-23 2017-09-25 Illumina Inc Methods and Compositions for Nucleic Acid Sequencing
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
KR20210080618A (ko) 2012-04-03 2021-06-30 일루미나, 인코포레이티드 핵산 서열분석에 유용한 통합 광전자 판독 헤드 및 유체 카트리지
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3572875A1 (en) 2013-12-19 2019-11-27 Illumina, Inc. Roll-to-roll process of preparing a patterned substrate and patterned substrate prepared by the same process
HK1225758A1 (zh) 2014-01-16 2017-09-15 Illumina, Inc. 固体支持物上的扩增子制备和测序
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

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