JP7014449B2 - 神経障害を処置するための間葉系細胞由来エキソソーム - Google Patents
神経障害を処置するための間葉系細胞由来エキソソーム Download PDFInfo
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| KR102506822B1 (ko) * | 2018-09-20 | 2023-03-06 | 시토스핀, 에스.엘. | 기능적 중간엽 줄기 세포의 농축된 집단을 수득하는 방법, 이의 수득된 세포 및 이를 포함하는 조성물 |
| EP3990625A4 (en) | 2019-06-26 | 2023-07-19 | Technion Research & Development Foundation Limited | PRODUCTION OF EXTRACELLULAR VESICLES FROM STEM CELLS |
| JP6830286B1 (ja) | 2020-06-26 | 2021-02-17 | デクソンファーマシューティカルズ株式会社 | 精巣機能改善剤および精巣機能改善方法 |
| CN115768871A (zh) | 2020-07-17 | 2023-03-07 | Dexon制药株式会社 | 组合物、结合抑制剂、医疗器械和covid-19的预防方法 |
| WO2022054565A1 (ja) | 2020-09-08 | 2022-03-17 | デクソンファーマシューティカルズ株式会社 | サイトカインストーム抑制剤、サイトカインストーム抑制剤の使用方法およびスクリーニング方法 |
| JP6974892B1 (ja) | 2021-05-27 | 2021-12-01 | デクソンファーマシューティカルズ株式会社 | 癌悪液質の改善剤および癌悪液質の改善方法 |
| JP7492757B2 (ja) | 2022-03-22 | 2024-05-30 | Dexonファーマシューティカルズ株式会社 | 遺伝子の発現制御剤、アルツハイマー病の予防薬または治療薬および認知症の改善方法 |
| IL316553A (en) | 2022-05-15 | 2024-12-01 | Nurexone Biologic Ltd | RNA interference oligonucleotides against PTEN and their use |
| CN115006427A (zh) * | 2022-05-31 | 2022-09-06 | 尧舜泽生物医药(南京)有限公司 | 骨髓间充质干细胞外泌体在治疗帕金森病中的应用 |
| CN119365204A (zh) | 2022-06-20 | 2025-01-24 | Dexon制药株式会社 | Hmgb1的表达调控剂;急性肺损伤、急性呼吸窘迫综合征或败血症的预防药或治疗药,或它们的改善方法 |
| WO2023248845A1 (ja) | 2022-06-20 | 2023-12-28 | Dexonファーマシューティカルズ株式会社 | Ptx3の発現制御剤;関節リウマチ、自己免疫疾患に伴う血管炎または皮膚硬化の予防薬または治療薬、あるいはそれらの改善方法 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110014251A1 (en) | 2008-01-04 | 2011-01-20 | Lydac Neuroscience Limited | Microvesicles |
| JP2011219432A (ja) | 2010-04-13 | 2011-11-04 | Nagoya Univ | 歯髄幹細胞を用いた神経疾患治療用組成物 |
| JP2012508733A (ja) | 2008-11-14 | 2012-04-12 | メディポスト カンパニー リミテッド | 間葉幹細胞またはその培養液を含む神経疾患の予防または治療用の組成物 |
| WO2012156968A2 (en) | 2011-05-19 | 2012-11-22 | Ariel - University Research And Development Company, Ltd. | Use of mesenchymal stem cells for the improvement of affective and cognitive function |
| JP2016065106A (ja) | 2010-03-26 | 2016-04-28 | 国立大学法人名古屋大学 | 損傷部治療用組成物の製造方法 |
| WO2016149358A1 (en) | 2015-03-16 | 2016-09-22 | Duncan Ross | Method of treatment comprising membrane-enclosed vesicle |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
| US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
| US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
| US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
| US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
| US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
| US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
| US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
| US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
| US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
| US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
| CN101184499B (zh) * | 2005-02-23 | 2012-03-28 | 阿尔扎公司 | 活性剂向中枢神经系统的鼻内施用 |
| WO2008036374A2 (en) * | 2006-09-21 | 2008-03-27 | Medistem Laboratories, Inc. | Allogeneic stem cell transplants in non-conditioned recipients |
| CN102014934B (zh) | 2008-02-22 | 2014-08-20 | 新加坡科技研究局 | 间充质干细胞颗粒 |
| JP5667180B2 (ja) * | 2009-07-01 | 2015-02-12 | イオン メディックス インコーポレイテッド | 哺乳類の有核細胞に由来するマイクロベシクル及びその用途 |
| GB201011589D0 (en) | 2010-07-09 | 2010-08-25 | Reneuron Ltd | Therapeutic cells |
| WO2012020307A2 (en) * | 2010-08-13 | 2012-02-16 | The University Court Of The University Of Glasgow | Therapeutic uses of microvesicles and related micrornas |
| GB2502704B (en) * | 2010-11-15 | 2019-12-04 | Accelerated Biosciences Corp | Generation of neural stem cells from human trophoblast stem cells |
| EP3401393B1 (en) * | 2012-02-22 | 2020-02-19 | Exostem Biotec Ltd | Micrornas for the generation of astrocytes |
| JP6450673B2 (ja) * | 2012-04-03 | 2019-01-09 | リニューロン・リミテッドReNeuron Limited | 幹細胞微粒子 |
| WO2013186735A2 (en) | 2012-06-14 | 2013-12-19 | Bar-Ilan Research And Development Company Ltd. | Photothermal detection |
| SG11201601939VA (en) * | 2013-09-16 | 2016-04-28 | Agency Science Tech & Res | Method |
-
2017
- 2017-08-14 SG SG11201901272WA patent/SG11201901272WA/en unknown
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- 2017-08-14 IL IL264825A patent/IL264825B/en unknown
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-
2021
- 2021-08-04 US US17/394,008 patent/US20210361717A1/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110014251A1 (en) | 2008-01-04 | 2011-01-20 | Lydac Neuroscience Limited | Microvesicles |
| JP2012508733A (ja) | 2008-11-14 | 2012-04-12 | メディポスト カンパニー リミテッド | 間葉幹細胞またはその培養液を含む神経疾患の予防または治療用の組成物 |
| JP2016065106A (ja) | 2010-03-26 | 2016-04-28 | 国立大学法人名古屋大学 | 損傷部治療用組成物の製造方法 |
| JP2011219432A (ja) | 2010-04-13 | 2011-11-04 | Nagoya Univ | 歯髄幹細胞を用いた神経疾患治療用組成物 |
| WO2012156968A2 (en) | 2011-05-19 | 2012-11-22 | Ariel - University Research And Development Company, Ltd. | Use of mesenchymal stem cells for the improvement of affective and cognitive function |
| WO2016149358A1 (en) | 2015-03-16 | 2016-09-22 | Duncan Ross | Method of treatment comprising membrane-enclosed vesicle |
Non-Patent Citations (3)
| Title |
|---|
| Autism Research,2015年08月10日,(2016) Vol. 9,pp. 17-32 |
| Journal of Cerebral Blood Flow & Metabolism,Vol. 33,2013年,pp. 1711-1715 |
| STEM CELLS TRANSLATIONAL MEDICINE,2015年,Vol. 4,pp. 1131-1143 |
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