JP6935945B2 - 細胞を完全に溶解するための併用式溶解プロトコル - Google Patents

細胞を完全に溶解するための併用式溶解プロトコル Download PDF

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JP6935945B2
JP6935945B2 JP2019556545A JP2019556545A JP6935945B2 JP 6935945 B2 JP6935945 B2 JP 6935945B2 JP 2019556545 A JP2019556545 A JP 2019556545A JP 2019556545 A JP2019556545 A JP 2019556545A JP 6935945 B2 JP6935945 B2 JP 6935945B2
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mixed solution
ionic surfactant
cells
sample
dna
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JP2020503068A5 (https=
JP2020503068A (ja
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マーク ドリスコル
マーク ドリスコル
トーマス ジャーヴィー
トーマス ジャーヴィー
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ショアライン バイオミー エルエルシー
ショアライン バイオミー エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019556545A 2016-12-29 2017-12-26 細胞を完全に溶解するための併用式溶解プロトコル Active JP6935945B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662440171P 2016-12-29 2016-12-29
US62/440,171 2016-12-29
PCT/US2017/068415 WO2018125865A1 (en) 2016-12-29 2017-12-26 Combined lysis protocol for comprehensive cell lysis

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JP2020503068A JP2020503068A (ja) 2020-01-30
JP2020503068A5 JP2020503068A5 (https=) 2021-02-12
JP6935945B2 true JP6935945B2 (ja) 2021-09-15

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US (2) US10774322B2 (https=)
EP (1) EP3562576B1 (https=)
JP (1) JP6935945B2 (https=)
KR (1) KR102281213B1 (https=)
CN (1) CN110312570B (https=)
AU (1) AU2017388204B2 (https=)
CA (1) CA3048844C (https=)
WO (1) WO2018125865A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11149246B2 (en) * 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
WO2020181082A1 (en) * 2019-03-07 2020-09-10 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight dna from modified cells
KR102370468B1 (ko) * 2020-03-18 2022-03-03 재단법인대구경북과학기술원 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도
EP4153606A4 (en) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES
CN112626174B (zh) * 2020-12-16 2024-05-07 广州源井生物科技有限公司 一种细胞裂解液及其应用

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
CA2319775C (en) * 1998-02-02 2009-05-05 Gentra Systems, Inc. Compositions and methods for using a lysing matrix for isolating dna
CN1057530C (zh) * 1998-02-12 2000-10-18 复旦大学 核酸快速抽提方法
CN1308130A (zh) * 2000-12-26 2001-08-15 李永武 从微生物细胞中制取核酸的方法
AU2003217552A1 (en) 2002-02-19 2003-09-09 Choicepoint Asset Company Selective extraction of dna from groups of cells
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1608969A4 (en) * 2003-03-19 2007-06-06 Alfa Wassermann Inc SEPARATION AND ACCUMULATION OF SUB-CELLULAR COMPONENTS, AND PROTEINS WHICH ARE DRAWN THEREFROM
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
EP1628749B1 (en) * 2003-05-30 2019-07-31 VGXI, Inc. Devices and methods for biomaterial production
WO2005073377A1 (ja) * 2004-01-28 2005-08-11 Toudai Tlo, Ltd. 環境サンプルからのｄｎａの回収方法
EP2333105A1 (en) * 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
CN102822339A (zh) * 2010-04-08 2012-12-12 恰根有限公司 在核酸存在下沉淀阴离子表面活性剂离子的方法
EP2576801B1 (en) * 2010-06-01 2019-10-02 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
JP5624487B2 (ja) * 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
CA2831969A1 (en) * 2011-06-06 2012-12-30 Biocartis S.A. Selective lysis of cells by ionic surfactants
WO2016024263A1 (en) 2014-08-14 2016-02-18 Molecular Detection Israel Ltd. Methods for isolating microbial dna from a blood sample
US20170166956A1 (en) 2015-12-11 2017-06-15 Shoreline Biome, Llc Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing

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Publication number Publication date
CN110312570A (zh) 2019-10-08
US10774322B2 (en) 2020-09-15
US20180187181A1 (en) 2018-07-05
CA3048844A1 (en) 2018-07-05
WO2018125865A1 (en) 2018-07-05
US20210032618A1 (en) 2021-02-04
CN110312570B (zh) 2022-04-05
WO2018125865A8 (en) 2019-08-22
KR102281213B1 (ko) 2021-07-22
EP3562576A4 (en) 2020-07-22
EP3562576B1 (en) 2023-09-06
KR20190100315A (ko) 2019-08-28
AU2017388204B2 (en) 2023-10-12
JP2020503068A (ja) 2020-01-30
EP3562576A1 (en) 2019-11-06
AU2017388204A1 (en) 2019-08-01
CA3048844C (en) 2023-12-12

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