EP1608969A4 - Separation and accumulation of subcellular components, and proteins derived therefrom - Google Patents

Separation and accumulation of subcellular components, and proteins derived therefrom

Info

Publication number
EP1608969A4
EP1608969A4 EP04757676A EP04757676A EP1608969A4 EP 1608969 A4 EP1608969 A4 EP 1608969A4 EP 04757676 A EP04757676 A EP 04757676A EP 04757676 A EP04757676 A EP 04757676A EP 1608969 A4 EP1608969 A4 EP 1608969A4
Authority
EP
European Patent Office
Prior art keywords
accumulation
separation
derived therefrom
proteins derived
subcellular components
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04757676A
Other languages
German (de)
French (fr)
Other versions
EP1608969A2 (en
Inventor
Zvi G Loewy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alfa Wassermann Inc
Original Assignee
Alfa Wassermann Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc filed Critical Alfa Wassermann Inc
Publication of EP1608969A2 publication Critical patent/EP1608969A2/en
Publication of EP1608969A4 publication Critical patent/EP1608969A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
EP04757676A 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom Withdrawn EP1608969A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US741313 1996-10-30
US45576703P 2003-03-19 2003-03-19
US455767P 2003-03-19
US74131303A 2003-12-19 2003-12-19
PCT/US2004/008655 WO2004083405A2 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom

Publications (2)

Publication Number Publication Date
EP1608969A2 EP1608969A2 (en) 2005-12-28
EP1608969A4 true EP1608969A4 (en) 2007-06-06

Family

ID=33032702

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04757676A Withdrawn EP1608969A4 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom

Country Status (6)

Country Link
EP (1) EP1608969A4 (en)
JP (1) JP2006520606A (en)
KR (1) KR20050119128A (en)
AU (1) AU2004221348A1 (en)
CA (1) CA2518555A1 (en)
WO (1) WO2004083405A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496400A1 (en) 2002-09-09 2004-03-18 Dana-Farber Cancer Institute, Inc. Bh3 peptides and method of use thereof
WO2007123791A2 (en) 2006-03-31 2007-11-01 Dana-Farber Cancer Institute Methods of determining cellular chemosensitivity
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CN104023551A (en) 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 Simultaneous purification of cell components
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
JP6352924B2 (en) 2012-09-19 2018-07-04 ダナ−ファーバー キャンサー インスティテュート， インコーポレイテッド Dynamic BH3 profiling
AU2014323526B2 (en) 2013-09-19 2020-07-23 Dana-Farber Cancer Institute, Inc. Methods of BH3 profiling
US9862936B2 (en) 2015-01-13 2018-01-09 Alfa Wassermann, Inc. Methods of purifying adeno-associated virus (AAV) and/or recombinant adeno-associated virus (rAAV) and gradients and flow-through buffers therefore
CA2983024A1 (en) 2015-04-27 2016-11-03 Dana-Farber Cancer Institute, Inc. Compositions and methods for assessing toxicity using dynamic bh3 profiling
KR20160133837A (en) 2015-05-13 2016-11-23 고려대학교 산학협력단 Apparatus for separating fine endoplasmic reticulum by electrophoresis sample pH adjustment
KR20160133826A (en) 2015-05-13 2016-11-23 고려대학교 산학협력단 Apparatus for separating fine endoplasmic reticulum using electrophoresis
KR102281213B1 (en) * 2016-12-29 2021-07-22 쇼어라인 바이옴, 엘엘씨 Combined lysis protocol for comprehensive cell lysis
US11149246B2 (en) 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
CN115698265A (en) 2020-04-24 2023-02-03 高丽大学校算学协力团 Method and apparatus for separating microvesicles
KR20240096513A (en) * 2021-10-12 2024-06-26 사이토누스 테라퓨틱스, 인크. Systems and methods for producing therapeutic cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217418A (en) * 1978-05-08 1980-08-12 Merck & Co., Inc. Recovery of small particles by flow centrifugation
WO1994027698A2 (en) * 1993-05-28 1994-12-08 Baxter International Inc. Continuous centrifugation process for the separation of biologic components from heterogeneous cell populations
WO1997021488A1 (en) * 1995-12-11 1997-06-19 Dendreon Corporation Cell separation composition, kit and method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989835A (en) * 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US20020115056A1 (en) * 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
DE10158860B4 (en) * 2001-11-30 2007-04-05 Bruker Daltonik Gmbh Mass spectrometric protein mixture analysis
US20040107057A1 (en) * 2002-03-22 2004-06-03 Capaldi Roderick A Enhanced protein separation and analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217418A (en) * 1978-05-08 1980-08-12 Merck & Co., Inc. Recovery of small particles by flow centrifugation
WO1994027698A2 (en) * 1993-05-28 1994-12-08 Baxter International Inc. Continuous centrifugation process for the separation of biologic components from heterogeneous cell populations
WO1997021488A1 (en) * 1995-12-11 1997-06-19 Dendreon Corporation Cell separation composition, kit and method

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CALDWELL S R ET AL: "Large scale purification process for recombinant NS1- OspA as a candidate vaccine for Lyme disease", BIOSEPARATION, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 6, no. 2, April 1996 (1996-04-01), pages 115 - 123, XP002116461, ISSN: 0923-179X *
GASSER K W ET AL: "SEPARATION OF CELL ORGANELLES IN DENSITY GRADIENTS BASED ON THEIR PERMEABILITY CHARACTERISTICS", ANALYTICAL BIOCHEMISTRY, vol. 171, no. 1, 1988, pages 41 - 46, XP009081575 *
HANSON BONNIE J ET AL: "A novel subfractionation approach for mitochondrial proteins: A three-dimensional mitochondrial proteome map", ELECTROPHORESIS, vol. 22, no. 5, March 2001 (2001-03-01), pages 950 - 959, XP002428087, ISSN: 0173-0835 *
MORAND J N ET AL: "A ONE-STEP TECHNIQUE FOR THE SUBCELLULAR FRACTIONATION OF TOTAL CELL HOMOGENATES", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 159, no. 1, 1986, pages 157 - 162, XP009081582, ISSN: 0003-2697 *
RAJAPAKSE N ET AL: "Isolation and characterization of intact mitochondria from neonatal rat brain.", BRAIN RESEARCH. BRAIN RESEARCH PROTOCOLS DEC 2001, vol. 8, no. 3, December 2001 (2001-12-01), pages 176 - 183, XP002428086, ISSN: 1385-299X *

Also Published As

Publication number Publication date
WO2004083405A2 (en) 2004-09-30
EP1608969A2 (en) 2005-12-28
WO2004083405A8 (en) 2005-11-17
KR20050119128A (en) 2005-12-20
CA2518555A1 (en) 2004-09-30
AU2004221348A1 (en) 2004-09-30
WO2004083405A3 (en) 2005-04-07
JP2006520606A (en) 2006-09-14

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