KR102281213B1 - 포괄적인 세포 용해를 위한 조합된 용해 프로토콜 - Google Patents

포괄적인 세포 용해를 위한 조합된 용해 프로토콜 Download PDF

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KR102281213B1
KR102281213B1 KR1020197021748A KR20197021748A KR102281213B1 KR 102281213 B1 KR102281213 B1 KR 102281213B1 KR 1020197021748 A KR1020197021748 A KR 1020197021748A KR 20197021748 A KR20197021748 A KR 20197021748A KR 102281213 B1 KR102281213 B1 KR 102281213B1
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mixed solution
ionic surfactant
base
sample
cell
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KR20190100315A (ko
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마크 드리스콜
토마스 자비
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쇼어라인 바이옴, 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
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  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020197021748A 2016-12-29 2017-12-26 포괄적인 세포 용해를 위한 조합된 용해 프로토콜 Active KR102281213B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662440171P 2016-12-29 2016-12-29
US62/440,171 2016-12-29
PCT/US2017/068415 WO2018125865A1 (en) 2016-12-29 2017-12-26 Combined lysis protocol for comprehensive cell lysis

Publications (2)

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KR20190100315A KR20190100315A (ko) 2019-08-28
KR102281213B1 true KR102281213B1 (ko) 2021-07-22

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KR1020197021748A Active KR102281213B1 (ko) 2016-12-29 2017-12-26 포괄적인 세포 용해를 위한 조합된 용해 프로토콜

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US (2) US10774322B2 (https=)
EP (1) EP3562576B1 (https=)
JP (1) JP6935945B2 (https=)
KR (1) KR102281213B1 (https=)
CN (1) CN110312570B (https=)
AU (1) AU2017388204B2 (https=)
CA (1) CA3048844C (https=)
WO (1) WO2018125865A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11149246B2 (en) * 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
WO2020181082A1 (en) * 2019-03-07 2020-09-10 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight dna from modified cells
KR102370468B1 (ko) * 2020-03-18 2022-03-03 재단법인대구경북과학기술원 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도
EP4153606A4 (en) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES
CN112626174B (zh) * 2020-12-16 2024-05-07 广州源井生物科技有限公司 一种细胞裂解液及其应用

Citations (2)

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WO2005073377A1 (ja) 2004-01-28 2005-08-11 Toudai Tlo, Ltd. 環境サンプルからのｄｎａの回収方法
JP2006517225A (ja) 2003-02-06 2006-07-20 ベクトン・ディキンソン・アンド・カンパニー 核酸抽出のための生物学的試料の化学処理および該処理用のキット

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CA2319775C (en) * 1998-02-02 2009-05-05 Gentra Systems, Inc. Compositions and methods for using a lysing matrix for isolating dna
CN1057530C (zh) * 1998-02-12 2000-10-18 复旦大学 核酸快速抽提方法
CN1308130A (zh) * 2000-12-26 2001-08-15 李永武 从微生物细胞中制取核酸的方法
AU2003217552A1 (en) 2002-02-19 2003-09-09 Choicepoint Asset Company Selective extraction of dna from groups of cells
EP1608969A4 (en) * 2003-03-19 2007-06-06 Alfa Wassermann Inc SEPARATION AND ACCUMULATION OF SUB-CELLULAR COMPONENTS, AND PROTEINS WHICH ARE DRAWN THEREFROM
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
EP1628749B1 (en) * 2003-05-30 2019-07-31 VGXI, Inc. Devices and methods for biomaterial production
EP2333105A1 (en) * 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
CN102822339A (zh) * 2010-04-08 2012-12-12 恰根有限公司 在核酸存在下沉淀阴离子表面活性剂离子的方法
EP2576801B1 (en) * 2010-06-01 2019-10-02 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
JP5624487B2 (ja) * 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
CA2831969A1 (en) * 2011-06-06 2012-12-30 Biocartis S.A. Selective lysis of cells by ionic surfactants
WO2016024263A1 (en) 2014-08-14 2016-02-18 Molecular Detection Israel Ltd. Methods for isolating microbial dna from a blood sample
US20170166956A1 (en) 2015-12-11 2017-06-15 Shoreline Biome, Llc Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing

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JP2006517225A (ja) 2003-02-06 2006-07-20 ベクトン・ディキンソン・アンド・カンパニー 核酸抽出のための生物学的試料の化学処理および該処理用のキット
WO2005073377A1 (ja) 2004-01-28 2005-08-11 Toudai Tlo, Ltd. 環境サンプルからのｄｎａの回収方法

Also Published As

Publication number Publication date
CN110312570A (zh) 2019-10-08
US10774322B2 (en) 2020-09-15
US20180187181A1 (en) 2018-07-05
CA3048844A1 (en) 2018-07-05
WO2018125865A1 (en) 2018-07-05
US20210032618A1 (en) 2021-02-04
CN110312570B (zh) 2022-04-05
WO2018125865A8 (en) 2019-08-22
EP3562576A4 (en) 2020-07-22
EP3562576B1 (en) 2023-09-06
KR20190100315A (ko) 2019-08-28
AU2017388204B2 (en) 2023-10-12
JP2020503068A (ja) 2020-01-30
EP3562576A1 (en) 2019-11-06
JP6935945B2 (ja) 2021-09-15
AU2017388204A1 (en) 2019-08-01
CA3048844C (en) 2023-12-12

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