AU2017388204B2 - Combined lysis protocol for comprehensive cell lysis - Google Patents
Combined lysis protocol for comprehensive cell lysis Download PDFInfo
- Publication number
- AU2017388204B2 AU2017388204B2 AU2017388204A AU2017388204A AU2017388204B2 AU 2017388204 B2 AU2017388204 B2 AU 2017388204B2 AU 2017388204 A AU2017388204 A AU 2017388204A AU 2017388204 A AU2017388204 A AU 2017388204A AU 2017388204 B2 AU2017388204 B2 AU 2017388204B2
- Authority
- AU
- Australia
- Prior art keywords
- lysis
- conducted
- ionic detergent
- base
- detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662440171P | 2016-12-29 | 2016-12-29 | |
| US62/440,171 | 2016-12-29 | ||
| PCT/US2017/068415 WO2018125865A1 (en) | 2016-12-29 | 2017-12-26 | Combined lysis protocol for comprehensive cell lysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2017388204A1 AU2017388204A1 (en) | 2019-08-01 |
| AU2017388204B2 true AU2017388204B2 (en) | 2023-10-12 |
Family
ID=62709305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2017388204A Active AU2017388204B2 (en) | 2016-12-29 | 2017-12-26 | Combined lysis protocol for comprehensive cell lysis |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10774322B2 (https=) |
| EP (1) | EP3562576B1 (https=) |
| JP (1) | JP6935945B2 (https=) |
| KR (1) | KR102281213B1 (https=) |
| CN (1) | CN110312570B (https=) |
| AU (1) | AU2017388204B2 (https=) |
| CA (1) | CA3048844C (https=) |
| WO (1) | WO2018125865A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10428370B2 (en) * | 2016-09-15 | 2019-10-01 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
| US11959125B2 (en) | 2016-09-15 | 2024-04-16 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
| US11149246B2 (en) * | 2016-12-29 | 2021-10-19 | Shoreline Biome, Llc | Methods for cell lysis and preparation of high molecular weight DNA from modified cells |
| WO2020181082A1 (en) * | 2019-03-07 | 2020-09-10 | Shoreline Biome, Llc | Methods for cell lysis and preparation of high molecular weight dna from modified cells |
| KR102370468B1 (ko) * | 2020-03-18 | 2022-03-03 | 재단법인대구경북과학기술원 | 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도 |
| EP4153606A4 (en) | 2020-07-13 | 2024-10-02 | Singular Genomics Systems, Inc. | METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES |
| CN112626174B (zh) * | 2020-12-16 | 2024-05-07 | 广州源井生物科技有限公司 | 一种细胞裂解液及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006517225A (ja) * | 2003-02-06 | 2006-07-20 | ベクトン・ディキンソン・アンド・カンパニー | 核酸抽出のための生物学的試料の化学処理および該処理用のキット |
| EP2481791A1 (en) * | 2011-01-31 | 2012-08-01 | Tokyo University of Agriculture & Technology | Nucleic acid extraction method |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2319775C (en) * | 1998-02-02 | 2009-05-05 | Gentra Systems, Inc. | Compositions and methods for using a lysing matrix for isolating dna |
| CN1057530C (zh) * | 1998-02-12 | 2000-10-18 | 复旦大学 | 核酸快速抽提方法 |
| CN1308130A (zh) * | 2000-12-26 | 2001-08-15 | 李永武 | 从微生物细胞中制取核酸的方法 |
| AU2003217552A1 (en) | 2002-02-19 | 2003-09-09 | Choicepoint Asset Company | Selective extraction of dna from groups of cells |
| EP1608969A4 (en) * | 2003-03-19 | 2007-06-06 | Alfa Wassermann Inc | SEPARATION AND ACCUMULATION OF SUB-CELLULAR COMPONENTS, AND PROTEINS WHICH ARE DRAWN THEREFROM |
| US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
| EP1628749B1 (en) * | 2003-05-30 | 2019-07-31 | VGXI, Inc. | Devices and methods for biomaterial production |
| WO2005073377A1 (ja) * | 2004-01-28 | 2005-08-11 | Toudai Tlo, Ltd. | 環境サンプルからのｄｎａの回収方法 |
| EP2333105A1 (en) * | 2009-12-08 | 2011-06-15 | Koninklijke Philips Electronics N.V. | Selective lysis of cells |
| CN102822339A (zh) * | 2010-04-08 | 2012-12-12 | 恰根有限公司 | 在核酸存在下沉淀阴离子表面活性剂离子的方法 |
| EP2576801B1 (en) * | 2010-06-01 | 2019-10-02 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| CA2831969A1 (en) * | 2011-06-06 | 2012-12-30 | Biocartis S.A. | Selective lysis of cells by ionic surfactants |
| WO2016024263A1 (en) | 2014-08-14 | 2016-02-18 | Molecular Detection Israel Ltd. | Methods for isolating microbial dna from a blood sample |
| US20170166956A1 (en) | 2015-12-11 | 2017-06-15 | Shoreline Biome, Llc | Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing |
-
2017
- 2017-12-26 AU AU2017388204A patent/AU2017388204B2/en active Active
- 2017-12-26 US US15/854,157 patent/US10774322B2/en active Active
- 2017-12-26 WO PCT/US2017/068415 patent/WO2018125865A1/en not_active Ceased
- 2017-12-26 EP EP17887015.0A patent/EP3562576B1/en active Active
- 2017-12-26 JP JP2019556545A patent/JP6935945B2/ja active Active
- 2017-12-26 CN CN201780085878.1A patent/CN110312570B/zh active Active
- 2017-12-26 KR KR1020197021748A patent/KR102281213B1/ko active Active
- 2017-12-26 CA CA3048844A patent/CA3048844C/en active Active
-
2020
- 2020-08-19 US US16/996,996 patent/US20210032618A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006517225A (ja) * | 2003-02-06 | 2006-07-20 | ベクトン・ディキンソン・アンド・カンパニー | 核酸抽出のための生物学的試料の化学処理および該処理用のキット |
| EP2481791A1 (en) * | 2011-01-31 | 2012-08-01 | Tokyo University of Agriculture & Technology | Nucleic acid extraction method |
Non-Patent Citations (1)
| Title |
|---|
| LEE ET AL. YEAST, vol. 29, February 2012, pages 73 - 80 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110312570A (zh) | 2019-10-08 |
| US10774322B2 (en) | 2020-09-15 |
| US20180187181A1 (en) | 2018-07-05 |
| CA3048844A1 (en) | 2018-07-05 |
| WO2018125865A1 (en) | 2018-07-05 |
| US20210032618A1 (en) | 2021-02-04 |
| CN110312570B (zh) | 2022-04-05 |
| WO2018125865A8 (en) | 2019-08-22 |
| KR102281213B1 (ko) | 2021-07-22 |
| EP3562576A4 (en) | 2020-07-22 |
| EP3562576B1 (en) | 2023-09-06 |
| KR20190100315A (ko) | 2019-08-28 |
| JP2020503068A (ja) | 2020-01-30 |
| EP3562576A1 (en) | 2019-11-06 |
| JP6935945B2 (ja) | 2021-09-15 |
| AU2017388204A1 (en) | 2019-08-01 |
| CA3048844C (en) | 2023-12-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |