JP2020503068A5 - - Google Patents
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- Publication number
- JP2020503068A5 JP2020503068A5 JP2019556545A JP2019556545A JP2020503068A5 JP 2020503068 A5 JP2020503068 A5 JP 2020503068A5 JP 2019556545 A JP2019556545 A JP 2019556545A JP 2019556545 A JP2019556545 A JP 2019556545A JP 2020503068 A5 JP2020503068 A5 JP 2020503068A5
- Authority
- JP
- Japan
- Prior art keywords
- mixed solution
- ionic surfactant
- base
- samples
- biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 31
- 239000011259 mixed solution Substances 0.000 claims 15
- 239000002563 ionic surfactant Substances 0.000 claims 12
- 239000000203 mixture Substances 0.000 claims 3
- 239000002244 precipitate Substances 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 230000002934 lysing effect Effects 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 241000203069 Archaea Species 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 claims 1
- 241000206602 Eukaryota Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 238000012408 PCR amplification Methods 0.000 claims 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical group [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims 1
- 206010036790 Productive cough Diseases 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- 238000001816 cooling Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 230000005484 gravity Effects 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 210000003097 mucus Anatomy 0.000 claims 1
- 230000001376 precipitating effect Effects 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 150000003839 salts Chemical group 0.000 claims 1
- 238000004062 sedimentation Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 210000003802 sputum Anatomy 0.000 claims 1
- 208000024794 sputum Diseases 0.000 claims 1
- 238000003860 storage Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- -1 tissues Substances 0.000 claims 1
- 210000002105 tongue Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662440171P | 2016-12-29 | 2016-12-29 | |
| US62/440,171 | 2016-12-29 | ||
| PCT/US2017/068415 WO2018125865A1 (en) | 2016-12-29 | 2017-12-26 | Combined lysis protocol for comprehensive cell lysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020503068A JP2020503068A (ja) | 2020-01-30 |
| JP2020503068A5 true JP2020503068A5 (https=) | 2021-02-12 |
| JP6935945B2 JP6935945B2 (ja) | 2021-09-15 |
Family
ID=62709305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019556545A Active JP6935945B2 (ja) | 2016-12-29 | 2017-12-26 | 細胞を完全に溶解するための併用式溶解プロトコル |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10774322B2 (https=) |
| EP (1) | EP3562576B1 (https=) |
| JP (1) | JP6935945B2 (https=) |
| KR (1) | KR102281213B1 (https=) |
| CN (1) | CN110312570B (https=) |
| AU (1) | AU2017388204B2 (https=) |
| CA (1) | CA3048844C (https=) |
| WO (1) | WO2018125865A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10428370B2 (en) * | 2016-09-15 | 2019-10-01 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
| US11959125B2 (en) | 2016-09-15 | 2024-04-16 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
| US11149246B2 (en) * | 2016-12-29 | 2021-10-19 | Shoreline Biome, Llc | Methods for cell lysis and preparation of high molecular weight DNA from modified cells |
| WO2020181082A1 (en) * | 2019-03-07 | 2020-09-10 | Shoreline Biome, Llc | Methods for cell lysis and preparation of high molecular weight dna from modified cells |
| KR102370468B1 (ko) * | 2020-03-18 | 2022-03-03 | 재단법인대구경북과학기술원 | 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도 |
| EP4153606A4 (en) | 2020-07-13 | 2024-10-02 | Singular Genomics Systems, Inc. | METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES |
| CN112626174B (zh) * | 2020-12-16 | 2024-05-07 | 广州源井生物科技有限公司 | 一种细胞裂解液及其应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2319775C (en) * | 1998-02-02 | 2009-05-05 | Gentra Systems, Inc. | Compositions and methods for using a lysing matrix for isolating dna |
| CN1057530C (zh) * | 1998-02-12 | 2000-10-18 | 复旦大学 | 核酸快速抽提方法 |
| CN1308130A (zh) * | 2000-12-26 | 2001-08-15 | 李永武 | 从微生物细胞中制取核酸的方法 |
| AU2003217552A1 (en) | 2002-02-19 | 2003-09-09 | Choicepoint Asset Company | Selective extraction of dna from groups of cells |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| EP1608969A4 (en) * | 2003-03-19 | 2007-06-06 | Alfa Wassermann Inc | SEPARATION AND ACCUMULATION OF SUB-CELLULAR COMPONENTS, AND PROTEINS WHICH ARE DRAWN THEREFROM |
| US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
| EP1628749B1 (en) * | 2003-05-30 | 2019-07-31 | VGXI, Inc. | Devices and methods for biomaterial production |
| WO2005073377A1 (ja) * | 2004-01-28 | 2005-08-11 | Toudai Tlo, Ltd. | 環境サンプルからのｄｎａの回収方法 |
| EP2333105A1 (en) * | 2009-12-08 | 2011-06-15 | Koninklijke Philips Electronics N.V. | Selective lysis of cells |
| CN102822339A (zh) * | 2010-04-08 | 2012-12-12 | 恰根有限公司 | 在核酸存在下沉淀阴离子表面活性剂离子的方法 |
| EP2576801B1 (en) * | 2010-06-01 | 2019-10-02 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| JP5624487B2 (ja) * | 2011-01-31 | 2014-11-12 | 横河電機株式会社 | 核酸抽出方法 |
| CA2831969A1 (en) * | 2011-06-06 | 2012-12-30 | Biocartis S.A. | Selective lysis of cells by ionic surfactants |
| WO2016024263A1 (en) | 2014-08-14 | 2016-02-18 | Molecular Detection Israel Ltd. | Methods for isolating microbial dna from a blood sample |
| US20170166956A1 (en) | 2015-12-11 | 2017-06-15 | Shoreline Biome, Llc | Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing |
-
2017
- 2017-12-26 AU AU2017388204A patent/AU2017388204B2/en active Active
- 2017-12-26 US US15/854,157 patent/US10774322B2/en active Active
- 2017-12-26 WO PCT/US2017/068415 patent/WO2018125865A1/en not_active Ceased
- 2017-12-26 EP EP17887015.0A patent/EP3562576B1/en active Active
- 2017-12-26 JP JP2019556545A patent/JP6935945B2/ja active Active
- 2017-12-26 CN CN201780085878.1A patent/CN110312570B/zh active Active
- 2017-12-26 KR KR1020197021748A patent/KR102281213B1/ko active Active
- 2017-12-26 CA CA3048844A patent/CA3048844C/en active Active
-
2020
- 2020-08-19 US US16/996,996 patent/US20210032618A1/en not_active Abandoned
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