JP6885876B2 - ゲノムdnaを改変するための方法および組成物 - Google Patents
ゲノムdnaを改変するための方法および組成物 Download PDFInfo
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- JP6885876B2 JP6885876B2 JP2017554057A JP2017554057A JP6885876B2 JP 6885876 B2 JP6885876 B2 JP 6885876B2 JP 2017554057 A JP2017554057 A JP 2017554057A JP 2017554057 A JP2017554057 A JP 2017554057A JP 6885876 B2 JP6885876 B2 JP 6885876B2
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2818—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0602—Vertebrate cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N2510/00—Genetically modified cells
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562146618P | 2015-04-13 | 2015-04-13 | |
| US62/146,618 | 2015-04-13 | ||
| PCT/US2016/027253 WO2016168275A1 (en) | 2015-04-13 | 2016-04-13 | Methods and compositions for modifying genomic dna |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018512162A JP2018512162A (ja) | 2018-05-17 |
| JP2018512162A5 JP2018512162A5 (enExample) | 2019-05-23 |
| JP6885876B2 true JP6885876B2 (ja) | 2021-06-16 |
Family
ID=55806847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017554057A Active JP6885876B2 (ja) | 2015-04-13 | 2016-04-13 | ゲノムdnaを改変するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US11608511B2 (enExample) |
| EP (1) | EP3283635B1 (enExample) |
| JP (1) | JP6885876B2 (enExample) |
| KR (2) | KR20250058073A (enExample) |
| CN (1) | CN107980059B (enExample) |
| CA (1) | CA2982759C (enExample) |
| ES (1) | ES2926467T3 (enExample) |
| WO (1) | WO2016168275A1 (enExample) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2963693A1 (en) | 2014-10-10 | 2016-04-14 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| CA3047313A1 (en) | 2016-12-16 | 2018-06-21 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| CA3248436A1 (en) | 2017-09-01 | 2025-02-21 | Lonza Walkersville, Inc. | END-TO-END AUTOMATION OF CELL THERAPY |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
| WO2019190874A1 (en) | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| CA3104288A1 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| EP3856922A4 (en) | 2018-09-28 | 2022-07-06 | Octane Biotech Inc. | MAGNETIC SEPARATION |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | GMO ENZYMES |
| WO2020132177A1 (en) | 2018-12-21 | 2020-06-25 | Lonza Walkersville, Inc. | Automated production of viral vectors |
| CA3123314A1 (en) | 2018-12-21 | 2020-06-25 | Octane Biotech Inc. | Carousel for modular biologic production units |
| EP3914690A4 (en) | 2019-02-08 | 2022-11-09 | Lonza Walkersville, Inc. | METHODS AND DEVICES FOR DETERMINING CELL CONCENTRATION IN AUTOMATED BIOREACTORS |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113939593A (zh) | 2019-06-06 | 2022-01-14 | 因思科瑞普特公司 | 用于递归的核酸指导的细胞编辑的处治 |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| KR20220122975A (ko) | 2019-10-24 | 2022-09-05 | 론자 워커스빌 아이엔씨. | 개선된 세포-접촉 표면을 갖는 세포 배양 챔버 |
| EP4058563A4 (en) | 2019-11-11 | 2023-12-06 | Lonza Walkersville, Inc. | QUALITY CONTROL PROCEDURES FOR AUTOMATED CELL PROCESSING |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| US10883095B1 (en) | 2019-12-10 | 2021-01-05 | Inscripta, Inc. | Mad nucleases |
| US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP2023507566A (ja) | 2019-12-18 | 2023-02-24 | インスクリプタ, インコーポレイテッド | 核酸誘導ヌクレアーゼ編集済み細胞のin vivo検出のためのカスケード/dCas3相補性アッセイ |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| CN112195193A (zh) * | 2020-09-30 | 2021-01-08 | 上海交通大学 | 单链dna在基因转染中的应用 |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| AU2021415461A1 (en) | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554101A (en) | 1981-01-09 | 1985-11-19 | New York Blood Center, Inc. | Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity |
| EP0533838B1 (en) | 1990-06-11 | 1997-12-03 | NeXstar Pharmaceuticals, Inc. | Nucleic acid ligands |
| ATE272311T1 (de) | 1993-03-23 | 2004-08-15 | Cbr Lab Inc | Verfahren und vorrichtung zur einkapselung biologisch aktiver substanzen in zellen |
| JPH0844143A (ja) | 1993-06-29 | 1996-02-16 | Toshiba Corp | 帯電装置並びに該帯電装置を備えた画像形成装置 |
| EP0814855A4 (en) | 1995-03-10 | 2002-07-17 | Theramed Inc | FLOW ELECTROPORATION CHAMBER AND ASSOCIATED METHOD |
| US6074605A (en) | 1995-03-10 | 2000-06-13 | Entremed, Inc. | Flow electroporation chamber and method |
| US5720921A (en) | 1995-03-10 | 1998-02-24 | Entremed, Inc. | Flow electroporation chamber and method |
| US6773669B1 (en) | 1995-03-10 | 2004-08-10 | Maxcyte, Inc. | Flow electroporation chamber and method |
| US7067318B2 (en) * | 1995-06-07 | 2006-06-27 | The Regents Of The University Of Michigan | Methods for transfecting T cells |
| JP4031033B2 (ja) * | 1995-05-04 | 2008-01-09 | アメリカ合衆国 | T細胞の改良トランスフェクション法 |
| US6090617A (en) | 1996-12-05 | 2000-07-18 | Entremed, Inc. | Flow electroporation chamber with electrodes having a crystalline metal nitride coating |
| EP0972445B1 (en) | 1997-02-28 | 2006-07-05 | Kirin Beer Kabushiki Kaisha | Chimeric mice that express a human antibody |
| AU2001289104A1 (en) | 2000-09-15 | 2002-03-26 | Deltagen, Inc. | Methods of producing cells and animals comprising targeted gene modifications |
| WO2002026966A2 (en) | 2000-09-29 | 2002-04-04 | Ecole Polytechnique Federale De Lausanne | Transfection using plasmid preparations |
| US7029916B2 (en) | 2001-02-21 | 2006-04-18 | Maxcyte, Inc. | Apparatus and method for flow electroporation of biological samples |
| EP1456345B1 (en) | 2001-08-22 | 2016-07-20 | Maxcyte, Inc. | Apparatus and method for electroporation of biological samples |
| WO2004031353A2 (en) | 2002-09-30 | 2004-04-15 | Maxcyte, Inc. | Apparatus and method for streaming electroporation |
| JP2006518219A (ja) | 2003-02-18 | 2006-08-10 | マックスサイト インコーポレーティッド | 電気穿孔法による細胞への抗原の負荷方法 |
| US8409861B2 (en) * | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| AU2005245871A1 (en) | 2004-05-12 | 2005-12-01 | Maxcyte, Inc. | Methods and devices related to a regulated flow electroporation chamber |
| EP1802646B1 (en) | 2004-07-23 | 2017-09-06 | Immunomedics Inc. | Methods and compositions for increasing longevity and protein yield from a cell culture |
| US7531327B2 (en) | 2004-07-23 | 2009-05-12 | Immunomedics, Inc. | Methods and compositions for increasing longevity and protein yield from a cell culture |
| US20070059833A1 (en) | 2005-09-07 | 2007-03-15 | Maxcyte, Inc. | Use of Nucleases to Improve Viability and Enhance Transgene Expression in Transfected Cells |
| EP2010668A4 (en) | 2006-04-10 | 2009-04-29 | Univ California | SYSTEMS AND METHOD FOR THE EFFICIENT COLLECTION OF INDIVIDUAL CELLS AND CELL COLONIES AND FOR THE QUICK PRODUCTION OF STABILIZED TRANSFECTANTS |
| AU2008262487B2 (en) | 2007-05-23 | 2013-10-31 | Sangamo Therapeutics, Inc. | Methods and compositions for increased transgene expression |
| JP6039552B2 (ja) | 2010-07-01 | 2016-12-07 | グラクソ グループ リミテッドGlaxo Group Limited | 高産生細胞株を選択するための改良された方法 |
| JP2013537410A (ja) | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | 標的化エンドヌクレアーゼおよび一本鎖核酸を用いたゲノム編集 |
| CA2852955C (en) | 2011-10-27 | 2021-02-16 | Sangamo Biosciences, Inc. | Methods and compositions for modification of the hprt locus |
| WO2013139994A1 (en) | 2012-03-23 | 2013-09-26 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | A novel method of producing an oocyte carrying a modified target sequence in its genome |
| CA2871524C (en) * | 2012-05-07 | 2021-07-27 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease-mediated targeted integration of transgenes |
| CA2876076A1 (en) | 2012-06-12 | 2013-12-19 | Soren WARMING | Methods and compositions for generating conditional knock-out alleles |
| CN102839214A (zh) | 2012-09-06 | 2012-12-26 | 许汉鹏 | 筛选真核细胞基因组内特定基因调控相关机制和分子的方法 |
| JP6401704B2 (ja) * | 2012-10-10 | 2018-10-10 | サンガモ セラピューティクス, インコーポレイテッド | T細胞を修飾する化合物およびその使用 |
| CN105378071B (zh) | 2013-03-15 | 2021-04-20 | 美克斯细胞有限公司 | 用于产生稳定转染细胞的方法和组合物 |
| CN103224947B (zh) * | 2013-04-28 | 2015-06-10 | 陕西师范大学 | 一种基因打靶系统 |
| ES2883131T3 (es) * | 2013-05-29 | 2021-12-07 | Cellectis | Métodos para la modificación de células T para inmunoterapia utilizando el sistema de nucleasa CAS guiado por ARN |
| US9890393B2 (en) * | 2013-05-29 | 2018-02-13 | Cellectis | Methods for engineering T cells for immunotherapy by using RNA-guided CAS nuclease system |
| CA2915837A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| ES2962509T3 (es) | 2014-04-14 | 2024-03-19 | Maxcyte Inc | Métodos y composiciones para modificar ADN genómico |
| US10570418B2 (en) | 2014-09-02 | 2020-02-25 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification |
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2016
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- 2016-04-13 JP JP2017554057A patent/JP6885876B2/ja active Active
- 2016-04-13 KR KR1020257013453A patent/KR20250058073A/ko active Pending
- 2016-04-13 CA CA2982759A patent/CA2982759C/en active Active
- 2016-04-13 WO PCT/US2016/027253 patent/WO2016168275A1/en not_active Ceased
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| KR20250058073A (ko) | 2025-04-29 |
| ES2926467T3 (es) | 2022-10-26 |
| JP2018512162A (ja) | 2018-05-17 |
| US11608511B2 (en) | 2023-03-21 |
| CN107980059B (zh) | 2022-11-11 |
| US20180112235A1 (en) | 2018-04-26 |
| KR20170135966A (ko) | 2017-12-08 |
| WO2016168275A1 (en) | 2016-10-20 |
| CN107980059A (zh) | 2018-05-01 |
| EP3283635B1 (en) | 2022-07-06 |
| CA2982759C (en) | 2025-05-20 |
| EP3283635A1 (en) | 2018-02-21 |
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