JP2018512162A5 - - Google Patents
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- JP2018512162A5 JP2018512162A5 JP2017554057A JP2017554057A JP2018512162A5 JP 2018512162 A5 JP2018512162 A5 JP 2018512162A5 JP 2017554057 A JP2017554057 A JP 2017554057A JP 2017554057 A JP2017554057 A JP 2017554057A JP 2018512162 A5 JP2018512162 A5 JP 2018512162A5
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- JP
- Japan
- Prior art keywords
- cells
- dna
- cell
- sequence
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 94
- 210000004027 cell Anatomy 0.000 claims description 91
- 108020004414 DNA Proteins 0.000 claims description 65
- 239000000203 mixture Substances 0.000 claims description 34
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 28
- 230000004048 modification Effects 0.000 claims description 26
- 238000012986 modification Methods 0.000 claims description 26
- 238000001890 transfection Methods 0.000 claims description 21
- 230000003213 activating effect Effects 0.000 claims description 15
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims description 14
- 150000007523 nucleic acids Chemical group 0.000 claims description 12
- 239000013612 plasmid Substances 0.000 claims description 10
- 238000004520 electroporation Methods 0.000 claims description 8
- 101710163270 Nuclease Proteins 0.000 claims description 7
- 206010028980 Neoplasm Diseases 0.000 claims description 6
- 230000004075 alteration Effects 0.000 claims description 6
- 201000011510 cancer Diseases 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 210000004962 mammalian cell Anatomy 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 4
- 108700019146 Transgenes Proteins 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 210000004986 primary T-cell Anatomy 0.000 claims description 3
- 230000003612 virological effect Effects 0.000 claims description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 2
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 claims description 2
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 claims description 2
- 108091033409 CRISPR Proteins 0.000 claims description 2
- 108020004705 Codon Proteins 0.000 claims description 2
- 108020005004 Guide RNA Proteins 0.000 claims description 2
- 108010061833 Integrases Proteins 0.000 claims description 2
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 claims description 2
- 238000010459 TALEN Methods 0.000 claims description 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims description 2
- 102000008579 Transposases Human genes 0.000 claims description 2
- 108010020764 Transposases Proteins 0.000 claims description 2
- 238000002617 apheresis Methods 0.000 claims description 2
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 238000012217 deletion Methods 0.000 claims description 2
- 230000037430 deletion Effects 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 claims description 2
- 208000032839 leukemia Diseases 0.000 claims description 2
- 230000036210 malignancy Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 2
- 210000000822 natural killer cell Anatomy 0.000 claims description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 claims description 2
- 102100034343 Integrase Human genes 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000003833 cell viability Effects 0.000 description 3
- 210000002865 immune cell Anatomy 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 102000012330 Integrases Human genes 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562146618P | 2015-04-13 | 2015-04-13 | |
| US62/146,618 | 2015-04-13 | ||
| PCT/US2016/027253 WO2016168275A1 (en) | 2015-04-13 | 2016-04-13 | Methods and compositions for modifying genomic dna |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018512162A JP2018512162A (ja) | 2018-05-17 |
| JP2018512162A5 true JP2018512162A5 (enExample) | 2019-05-23 |
| JP6885876B2 JP6885876B2 (ja) | 2021-06-16 |
Family
ID=55806847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017554057A Active JP6885876B2 (ja) | 2015-04-13 | 2016-04-13 | ゲノムｄｎａを改変するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US11608511B2 (enExample) |
| EP (1) | EP3283635B1 (enExample) |
| JP (1) | JP6885876B2 (enExample) |
| KR (2) | KR20250058073A (enExample) |
| CN (1) | CN107980059B (enExample) |
| CA (1) | CA2982759C (enExample) |
| ES (1) | ES2926467T3 (enExample) |
| WO (1) | WO2016168275A1 (enExample) |
Families Citing this family (52)
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| CA2963693A1 (en) | 2014-10-10 | 2016-04-14 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
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| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| CA3047313A1 (en) | 2016-12-16 | 2018-06-21 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| CA3248436A1 (en) | 2017-09-01 | 2025-02-21 | Lonza Walkersville, Inc. | END-TO-END AUTOMATION OF CELL THERAPY |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
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| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| CA3104288A1 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| EP3856922A4 (en) | 2018-09-28 | 2022-07-06 | Octane Biotech Inc. | MAGNETIC SEPARATION |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | GMO ENZYMES |
| WO2020132177A1 (en) | 2018-12-21 | 2020-06-25 | Lonza Walkersville, Inc. | Automated production of viral vectors |
| CA3123314A1 (en) | 2018-12-21 | 2020-06-25 | Octane Biotech Inc. | Carousel for modular biologic production units |
| EP3914690A4 (en) | 2019-02-08 | 2022-11-09 | Lonza Walkersville, Inc. | METHODS AND DEVICES FOR DETERMINING CELL CONCENTRATION IN AUTOMATED BIOREACTORS |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113939593A (zh) | 2019-06-06 | 2022-01-14 | 因思科瑞普特公司 | 用于递归的核酸指导的细胞编辑的处治 |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| KR20220122975A (ko) | 2019-10-24 | 2022-09-05 | 론자 워커스빌 아이엔씨. | 개선된 세포-접촉 표면을 갖는 세포 배양 챔버 |
| EP4058563A4 (en) | 2019-11-11 | 2023-12-06 | Lonza Walkersville, Inc. | QUALITY CONTROL PROCEDURES FOR AUTOMATED CELL PROCESSING |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| US10883095B1 (en) | 2019-12-10 | 2021-01-05 | Inscripta, Inc. | Mad nucleases |
| US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP2023507566A (ja) | 2019-12-18 | 2023-02-24 | インスクリプタ， インコーポレイテッド | 核酸誘導ヌクレアーゼ編集済み細胞のｉｎ ｖｉｖｏ検出のためのカスケード／ｄＣａｓ３相補性アッセイ |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| CN112195193A (zh) * | 2020-09-30 | 2021-01-08 | 上海交通大学 | 单链dna在基因转染中的应用 |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| AU2021415461A1 (en) | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
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-
2016
- 2016-04-13 EP EP16718143.7A patent/EP3283635B1/en active Active
- 2016-04-13 JP JP2017554057A patent/JP6885876B2/ja active Active
- 2016-04-13 KR KR1020257013453A patent/KR20250058073A/ko active Pending
- 2016-04-13 CA CA2982759A patent/CA2982759C/en active Active
- 2016-04-13 WO PCT/US2016/027253 patent/WO2016168275A1/en not_active Ceased
- 2016-04-13 CN CN201680031891.4A patent/CN107980059B/zh active Active
- 2016-04-13 KR KR1020177032721A patent/KR20170135966A/ko not_active Ceased
- 2016-04-13 US US15/566,320 patent/US11608511B2/en active Active
- 2016-04-13 ES ES16718143T patent/ES2926467T3/es active Active
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