JP6646571B2 - 膵臓がんを検出するための方法および組成物 - Google Patents
膵臓がんを検出するための方法および組成物 Download PDFInfo
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- JP6646571B2 JP6646571B2 JP2016502266A JP2016502266A JP6646571B2 JP 6646571 B2 JP6646571 B2 JP 6646571B2 JP 2016502266 A JP2016502266 A JP 2016502266A JP 2016502266 A JP2016502266 A JP 2016502266A JP 6646571 B2 JP6646571 B2 JP 6646571B2
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| US201361780574P | 2013-03-13 | 2013-03-13 | |
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| PCT/US2014/026857 WO2014160499A2 (en) | 2013-03-13 | 2014-03-13 | Methods and compositions for detecting pancreatic cancer |
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| JP2020001856A Division JP7109008B2 (ja) | 2013-03-13 | 2020-01-09 | 膵臓がんを検出するための方法および組成物 |
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Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2347775T3 (da) | 2005-12-13 | 2020-07-13 | Harvard College | Skabeloner til celletransplantation |
| WO2008117067A2 (en) | 2007-03-27 | 2008-10-02 | Carl Arne Krister Borrebaeck | Protein signature/markers for the detection of adenocarcinoma |
| CN107648668B (zh) | 2010-10-06 | 2021-06-18 | 哈佛学院董事会 | 用于基于材料的细胞疗法的可注射的成孔水凝胶 |
| US9675561B2 (en) | 2011-04-28 | 2017-06-13 | President And Fellows Of Harvard College | Injectable cryogel vaccine devices and methods of use thereof |
| CA2848162C (en) | 2011-09-12 | 2023-03-14 | Creatics Llc | Non-invasive methods of detecting target molecules |
| WO2013152860A2 (en) * | 2012-04-11 | 2013-10-17 | Dutalys Gmbh | Improved antibody light chains |
| CN104244929B (zh) | 2012-04-16 | 2017-04-05 | 哈佛学院董事会 | 用于调节免疫反应的介孔二氧化硅组合物 |
| KR20150023904A (ko) | 2012-06-27 | 2015-03-05 | 버그 엘엘씨 | 전립선암의 진단 및 치료에서의 마커의 용도 |
| SI2968434T1 (sl) | 2013-03-15 | 2017-11-30 | Shire Viropharma Incorporated, | C1-INH sestavki za uporabo pri preprečevanju in zdravljenju hereditarnega angioedema (HAE) |
| CA2952390A1 (en) * | 2013-06-20 | 2014-12-24 | The Trustees Of The University Of Pennsylvania | Methods for diagnosing pancreatic cancer |
| US10267806B2 (en) | 2014-04-04 | 2019-04-23 | Mayo Foundation For Medical Education And Research | Isotyping immunoglobulins using accurate molecular mass |
| US10682400B2 (en) | 2014-04-30 | 2020-06-16 | President And Fellows Of Harvard College | Combination vaccine devices and methods of killing cancer cells |
| WO2015182218A1 (ja) * | 2014-05-26 | 2015-12-03 | オリンパス株式会社 | 膵癌判定方法 |
| KR20160045547A (ko) * | 2014-10-17 | 2016-04-27 | 에스케이텔레콤 주식회사 | 췌장암 진단용 조성물 및 이를 이용한 췌장암 진단방법 |
| SG11201704660YA (en) | 2014-12-08 | 2017-07-28 | Berg Llc | Use of markers including filamin a in the diagnosis and treatment of prostate cancer |
| CN107257802B (zh) | 2014-12-19 | 2021-11-23 | 豪夫迈·罗氏有限公司 | 转谷氨酰胺酶底物的鉴别及其用途 |
| CA2971402C (en) | 2014-12-19 | 2023-08-01 | F. Hoffmann-La Roche Ag | Microbial transglutaminases, substrates therefor and methods for the use thereof |
| US11054425B2 (en) | 2014-12-19 | 2021-07-06 | Roche Sequencing Solutions, Inc. | System and method for identification and characterization of transglutaminase species |
| EP3250250A4 (en) | 2015-01-30 | 2019-05-22 | President and Fellows of Harvard College | PERITUMORAL AND INTRATUMORAL MATERIALS FOR CANCER THERAPY |
| US20160271120A1 (en) * | 2015-03-17 | 2016-09-22 | Oakdene Holdings Llc | Pharmaceutical composition for cleansing of the gastrointestinal tract |
| GB201505654D0 (en) * | 2015-04-01 | 2015-05-13 | Nordic Bioscience As | Immunoassay for collagen type VI sequence |
| US20180306788A1 (en) * | 2015-04-07 | 2018-10-25 | President And Fellows Of Harvard College | Compositions and methods for modulating hydroxylation of acc2 by phd3 |
| JP7094533B2 (ja) | 2015-04-10 | 2022-07-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 免疫細胞捕捉デバイスおよびその製造および使用方法 |
| WO2016169581A1 (en) * | 2015-04-20 | 2016-10-27 | Consejo Superior De Investigaciones Científicas | Agents binding specifically to human cadherin-17, human cadherin-5, human cadherin-6 and human cadherin-20 rgd motif |
| HK1254866A1 (zh) | 2015-06-25 | 2019-07-26 | Immatics Biotechnologies Gmbh | 用於骨髓瘤和其他癌症免疫治疗的新型细胞表位和细胞表位组合物 |
| GB201511191D0 (en) * | 2015-06-25 | 2015-08-12 | Immatics Biotechnologies Gmbh | T-cell epitopes for the immunotherapy of myeloma |
| CN108137661B (zh) * | 2015-09-03 | 2021-07-27 | 香港大学 | 用于在乳腺癌患者中预测他莫昔芬响应的单克隆抗体 |
| US11209439B2 (en) | 2015-09-24 | 2021-12-28 | Mayo Foundation For Medical Education And Research | Identification of immunoglobulin free light chains by mass spectrometry |
| CN105296613A (zh) * | 2015-09-24 | 2016-02-03 | 郑州市职业病防治院 | 一种人TERF1基因rs3863242位点多态性检测技术 |
| US11371099B2 (en) | 2015-11-30 | 2022-06-28 | Mayo Foundation For Medical Education And Research | HEATR1 as a marker for chemoresistance |
| CN105785013A (zh) * | 2016-04-21 | 2016-07-20 | 卢连伟 | 一种辅助检测胰腺癌的胶体金免疫层析试纸及其制备方法 |
| GB201608192D0 (en) * | 2016-05-10 | 2016-06-22 | Immunovia Ab | Method, array and use thereof |
| JP7075125B2 (ja) * | 2016-05-25 | 2022-05-25 | イマティクス バイオテクノロジーズ ゲーエムベーハー | 標的としてのおよび胆嚢がんおよび胆管がんおよびその他のがんに対する免疫療法で使用するための新規ペプチド、ペプチド組み合わせ |
| CN115537372A (zh) | 2016-07-13 | 2022-12-30 | 哈佛学院院长等 | 抗原呈递细胞模拟支架及其制备和使用方法 |
| EP3493842A4 (en) | 2016-08-02 | 2020-07-29 | President and Fellows of Harvard College | BIOMATERIALS TO MODULATE IMMUNE RESPONSES |
| US10955420B2 (en) | 2016-09-07 | 2021-03-23 | Mayo Foundation For Medical Education And Research | Identification and monitoring of cleaved immunoglobulins by molecular mass |
| CN107655985B (zh) * | 2017-08-25 | 2020-05-26 | 南京农业大学 | 一种基于lc-ms-ms技术的体内蛋白质营养的评价方法 |
| US12153052B2 (en) | 2017-09-13 | 2024-11-26 | Mayo Foundation For Medical Education And Research | Identification and monitoring of immunoglobulin J chains |
| US11946937B2 (en) | 2017-09-13 | 2024-04-02 | Mayo Foundation For Medical Education And Research | Identification and monitoring of apoptosis inhibitor of macrophage |
| WO2019195448A1 (en) * | 2018-04-03 | 2019-10-10 | Creatics Llc | Methods for cancer detection by evaluation of glycan-binding patterns of immunoglobulins in gastrointestinal lavage fluid samples |
| WO2020061129A1 (en) | 2018-09-19 | 2020-03-26 | President And Fellows Of Harvard College | Compositions and methods for labeling and modulation of cells in vitro and in vivo |
| US20210395736A1 (en) * | 2018-10-29 | 2021-12-23 | The Johns Hopkins University | Rasopathy treatment |
| WO2020223646A1 (en) * | 2019-05-02 | 2020-11-05 | Creatics Llc | Methods for cancer detection by evaluation of alpha-1-antitrypsin (aat) and chymotrypsin-like elastase in gastrointestinal lavage fluid samples |
| CN114096555A (zh) * | 2019-06-24 | 2022-02-25 | 尔特斯特公司 | 新型胰腺癌诊断标志物 |
| US20220397576A1 (en) * | 2019-10-18 | 2022-12-15 | Reccan Diagnostics Ab | Apparatuses and methods for detection of pancreatic cancer |
| JP7693718B2 (ja) | 2020-05-26 | 2025-06-17 | エフ. ホフマン-ラ ロシュ アーゲー | 定量器-確認器比チェックのために顧客質量分析機器を較正するためのコンピュータ実装方法 |
| GB202010970D0 (en) | 2020-07-16 | 2020-09-02 | Immunovia Ab | Methods, arrays and uses thereof |
| KR102448586B1 (ko) * | 2020-10-08 | 2022-09-27 | 가톨릭대학교 산학협력단 | 췌장암 환자의 항암제 치료 반응성 예측용 마커 |
| EP4259201A4 (en) * | 2020-12-08 | 2025-03-19 | Memorial Sloan Kettering Cancer Center | Antibodies to galectin-3 and methods of use thereof |
| CN112461915B (zh) * | 2020-12-08 | 2024-07-12 | 杭州汇健科技有限公司 | 一种与药物敏感相关的特征分子筛选、药敏程度检测、细胞亚型检测的方法 |
| CA3202255A1 (en) | 2020-12-21 | 2022-06-30 | Hayley WARSINSKE | Markers for the early detection of colon cell proliferative disorders |
| WO2022171777A1 (en) * | 2021-02-12 | 2022-08-18 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for prognosis and treating a patient suffering from cancer |
| WO2022221177A1 (en) * | 2021-04-11 | 2022-10-20 | Khurana Vikas | Diagnosis and treatment of congestive colon failure (ccf) |
| AU2022291370A1 (en) * | 2021-06-07 | 2024-01-04 | Flagship Pioneering Innovations Vii, Llc | Compositions and methods for targeted delivery of therapeutic agents |
| WO2023044117A1 (en) * | 2021-09-20 | 2023-03-23 | Droplet Biosciences, Inc. | Drain fluid for diagnostics |
| US12152280B2 (en) | 2021-09-20 | 2024-11-26 | Droplet Biosciences, Inc. | Drain fluid for diagnostics |
| US12416626B2 (en) | 2022-07-22 | 2025-09-16 | Droplet Biosciences, Inc. | Methods for evaluating candidate therapy efficacy based on changes in lymphatic fluid biomarkers |
| WO2024086298A2 (en) * | 2022-10-20 | 2024-04-25 | The Johns Hopkins University | Hla class i and class ii-restricted t-cell epitopes in pancreatic cancer tissues and uses thereof |
| WO2024206856A2 (en) * | 2023-03-30 | 2024-10-03 | Thomas Jefferson University | Dna-dependent synthesis of rna by dna polymerase theta variants |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3276968D1 (en) * | 1982-08-09 | 1987-09-17 | Centocor Inc | Immunoassay for carbohydrate antigenic determinant |
| JPH0673470B2 (ja) * | 1986-07-15 | 1994-09-21 | 協和醗酵工業株式会社 | 抗ヒト胃癌単クロ−ン性抗体amc−462 |
| US5359681A (en) | 1993-01-11 | 1994-10-25 | University Of Washington | Fiber optic sensor and methods and apparatus relating thereto |
| US6924153B1 (en) | 1997-03-06 | 2005-08-02 | Quidel Corporation | Quantitative lateral flow assays and devices |
| JPH11225800A (ja) * | 1998-02-10 | 1999-08-24 | Kureha Chem Ind Co Ltd | 体液を用いた癌検出方法及びキット |
| US6447763B1 (en) * | 1998-06-12 | 2002-09-10 | Ian L. Gordon | Method and system for production and collection of lavage induced stool (LIS) for chemical and biologic tests of cells |
| WO2002002762A1 (en) * | 2000-07-03 | 2002-01-10 | Mochida Pharmaceutical Co., Ltd. | Novel lipase |
| WO2003014298A2 (en) * | 2001-08-03 | 2003-02-20 | Origene Technologies, Inc. | Full-length prostate selective polynucleotides and polypeptides |
| US7291324B2 (en) * | 2002-10-22 | 2007-11-06 | Braintree Laboratories Inc. | Method of bowel cleansing |
| WO2004055519A2 (en) * | 2002-12-17 | 2004-07-01 | Sinogenomax Co. Ltd. | Specific markers for pancreatic cancer |
| WO2005053512A2 (en) * | 2003-11-26 | 2005-06-16 | Dana-Farber Cancer Institute, Inc. | Animal models of pancreatic adenocarcinoma and uses therefor |
| WO2005114221A2 (en) | 2004-05-21 | 2005-12-01 | The Institute For Systems Biology | Compositions and methods for quantification of serum glycoproteins |
| US20090155799A1 (en) | 2006-03-02 | 2009-06-18 | Yusuke Nakamura | Methods for diagnosing pancreatic cancer using reg4 protein |
| WO2009102788A2 (en) * | 2008-02-15 | 2009-08-20 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| US20090258090A1 (en) | 2008-04-11 | 2009-10-15 | Braintree Laboratories, Inc. | Colon cleansing solution |
| CN102414565B (zh) * | 2009-05-04 | 2014-09-24 | 霍夫曼-拉罗奇有限公司 | 作为癌症的标记物的DPPIV/Seprase的用途 |
| WO2011027311A2 (en) * | 2009-09-03 | 2011-03-10 | Koninklijke Philips Electronics N.V. | Novel tumor markers |
| JP2013510094A (ja) * | 2009-11-05 | 2013-03-21 | ユニバーシティ オブ バージニア パテント ファウンデーション | がんのバイオマーカーとしてプレクチン−1を検出するための組成物および方法 |
| CA2800023A1 (en) * | 2010-06-01 | 2011-12-08 | Metanomics Health Gmbh | Means and methods for diagnosing pancreatic cancer in a subject |
| JP5931874B2 (ja) * | 2010-08-13 | 2016-06-08 | ソマロジック・インコーポレーテッド | 膵癌バイオマーカーおよびその使用 |
| AU2011351990A1 (en) * | 2010-12-29 | 2013-07-18 | Expression Pathology, Inc. | Protein biomarkers of recurrent breast cancer |
| CN103460045B (zh) * | 2011-04-05 | 2016-01-20 | 奥林巴斯株式会社 | 胰脏检查方法及胰脏检查试剂盒 |
| CA2848162C (en) * | 2011-09-12 | 2023-03-14 | Creatics Llc | Non-invasive methods of detecting target molecules |
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| AU2022224782A1 (en) | 2022-09-22 |
| AU2014243704A1 (en) | 2015-09-10 |
| EP2972375A2 (en) | 2016-01-20 |
| JP2020091295A (ja) | 2020-06-11 |
| CA2902841A1 (en) | 2014-10-02 |
| WO2014160499A3 (en) | 2015-01-29 |
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