JP6644547B2 - Macrophominaphaseolina由来のペクチン分解酵素およびその使用 - Google Patents

Macrophominaphaseolina由来のペクチン分解酵素およびその使用 Download PDF

Info

Publication number
JP6644547B2
JP6644547B2 JP2015527642A JP2015527642A JP6644547B2 JP 6644547 B2 JP6644547 B2 JP 6644547B2 JP 2015527642 A JP2015527642 A JP 2015527642A JP 2015527642 A JP2015527642 A JP 2015527642A JP 6644547 B2 JP6644547 B2 JP 6644547B2
Authority
JP
Japan
Prior art keywords
seq
sequence
phaseolina
dna
length
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015527642A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015529071A5 (ko
JP2015529071A (ja
Inventor
マクスドゥル アラム,
マクスドゥル アラム,
モハメド, シャヒドゥル イズラム,
モハメド, シャヒドゥル イズラム,
モハメド, モサデク ホッセン,
モハメド, モサデク ホッセン,
モハメド, サミウル ハク,
モハメド, サミウル ハク,
モハメド, モンジュール アラム,
モハメド, モンジュール アラム,
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bangladesh Jute Research Institute
Original Assignee
Bangladesh Jute Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bangladesh Jute Research Institute filed Critical Bangladesh Jute Research Institute
Publication of JP2015529071A publication Critical patent/JP2015529071A/ja
Publication of JP2015529071A5 publication Critical patent/JP2015529071A5/ja
Application granted granted Critical
Publication of JP6644547B2 publication Critical patent/JP6644547B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011Pectinesterase (3.1.1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01171Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
    • DTEXTILES; PAPER
    • D06TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06MTREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/02Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002Pectate lyase (4.2.2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/02Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02023Rhamnogalacturonan endolyase (4.2.2.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2015527642A 2012-08-16 2013-08-15 Macrophominaphaseolina由来のペクチン分解酵素およびその使用 Active JP6644547B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261683914P 2012-08-16 2012-08-16
US61/683,914 2012-08-16
PCT/US2013/055198 WO2014028772A2 (en) 2012-08-16 2013-08-15 Pectin degrading enzymes from macrophomina phaseolina and uses thereof

Publications (3)

Publication Number Publication Date
JP2015529071A JP2015529071A (ja) 2015-10-05
JP2015529071A5 JP2015529071A5 (ko) 2016-10-20
JP6644547B2 true JP6644547B2 (ja) 2020-02-12

Family

ID=50101609

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015527642A Active JP6644547B2 (ja) 2012-08-16 2013-08-15 Macrophominaphaseolina由来のペクチン分解酵素およびその使用

Country Status (16)

Country Link
US (1) US9765322B2 (ko)
EP (1) EP2885407B1 (ko)
JP (1) JP6644547B2 (ko)
KR (1) KR102043363B1 (ko)
CN (1) CN104837991A (ko)
AU (1) AU2013302535B2 (ko)
DK (1) DK2885407T3 (ko)
ES (1) ES2674922T3 (ko)
HK (1) HK1213597A1 (ko)
IN (1) IN2015DN02049A (ko)
MY (1) MY169809A (ko)
PL (1) PL2885407T3 (ko)
PT (1) PT2885407T (ko)
TR (1) TR201809053T4 (ko)
WO (1) WO2014028772A2 (ko)
ZA (1) ZA201501635B (ko)

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
EP0353188B2 (en) 1988-07-28 2002-03-06 Novartis AG Novel expression system
DE3908813A1 (de) 1989-03-17 1990-09-20 Roehm Gmbh Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus
IL108156A0 (en) 1992-12-23 1994-04-12 Novo Nordisk As An enzyme with polygalacturonase activity
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
EP1559776A3 (en) 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein
EP1683860B1 (en) * 1995-03-17 2013-10-23 Novozymes A/S Novel endoglucanases
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
CN1137254C (zh) 1997-04-09 2004-02-04 花王株式会社 洗涤剂组合物
US7214786B2 (en) * 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
ES2359381T5 (es) * 2002-05-14 2014-07-10 Novozymes A/S Variantes de pectato liasa
AU2003303934A1 (en) * 2002-05-30 2004-09-09 Dsm Ip Assets B.V. Novel pectinases and uses thereof
US10676751B2 (en) * 2008-02-29 2020-06-09 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
AR074835A1 (es) * 2008-12-19 2011-02-16 Danisco Proceso para la produccion de un producto de enzimas mediante la fermentacion sumergida con aspergillus
JPWO2010101158A1 (ja) 2009-03-02 2012-09-10 住友商事株式会社 クロストリジウムセルロボランス由来新規遺伝子及びその利用

Also Published As

Publication number Publication date
TR201809053T4 (tr) 2018-07-23
ZA201501635B (en) 2016-01-27
EP2885407B1 (en) 2018-06-13
ES2674922T3 (es) 2018-07-05
CN104837991A (zh) 2015-08-12
IN2015DN02049A (ko) 2015-08-14
WO2014028772A2 (en) 2014-02-20
EP2885407A4 (en) 2016-05-11
US9765322B2 (en) 2017-09-19
BR112015003086A2 (pt) 2018-06-26
PL2885407T3 (pl) 2018-09-28
DK2885407T3 (en) 2018-07-16
WO2014028772A3 (en) 2014-05-08
AU2013302535A1 (en) 2015-03-26
US20150259667A1 (en) 2015-09-17
KR102043363B1 (ko) 2019-11-11
HK1213597A1 (zh) 2016-07-08
KR20150042849A (ko) 2015-04-21
JP2015529071A (ja) 2015-10-05
AU2013302535B2 (en) 2018-12-06
EP2885407A2 (en) 2015-06-24
PT2885407T (pt) 2018-07-02
MY169809A (en) 2019-05-16

Similar Documents

Publication Publication Date Title
JP6663904B2 (ja) エラーを最少限に抑える核酸分子の合成のための材料及び方法
JP2018525983A (ja) ゲノム編集システムおよび使用方法
Silva et al. Identification, expression, and characterization of a novel bacterial RGI lyase enzyme for the production of bio-functional fibers
KR20210005054A (ko) 점도 감소 표현형을 포함하는 사상성 진균 균주
JP6644547B2 (ja) Macrophominaphaseolina由来のペクチン分解酵素およびその使用
Zhang et al. Lipase diversity in glacier soil based on analysis of metagenomic DNA fragments and cell culture
CA3225082A1 (en) Enzymes with ruvc domains
JP2024501892A (ja) 新規の核酸誘導型ヌクレアーゼ
TWI512107B (zh) 具提升酶產量及活性之甘露聚醣酶
JP6616687B2 (ja) Macrophominaphaseolina由来のセルロースおよび/またはヘミセルロース分解酵素およびその使用
JP6944697B2 (ja) 糸状菌におけるゲノム編集を用いる多段階による多重変異株の製造方法
BR112015003086B1 (pt) Sequência de polinucleotídeo recombinante, vetor de expressão recombinante e célula hospedeira procariótica geneticamente engenheirada
EP1496117B1 (en) Selection marker gene
CN106119137A (zh) 一种改善丝状真菌蛋白分泌能力的方法
WO2016137008A1 (ja) マンナナーゼ及びその利用
BR112015003085B1 (pt) Molécula de ácido nucleico recombinante, constructo recombinante de expressão e célula hospedeira procariótica engenheirada geneticamente
CN117821422A (zh) 核酸内切酶iv突变体及其应用
WO2020081328A1 (en) Complement component 1s (c1s) deficient cells for production of vaccines and biopharmaceutical proteins

Legal Events

Date Code Title Description
A529 Written submission of copy of amendment under article 34 pct

Free format text: JAPANESE INTERMEDIATE CODE: A529

Effective date: 20150416

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160810

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160810

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160902

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170530

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170830

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171030

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20171030

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180306

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180706

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20180709

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20180820

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20180907

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20181026

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20190925

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191025

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200108

R150 Certificate of patent or registration of utility model

Ref document number: 6644547

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250