JP6563492B2 - 間葉系幹細胞を区別する方法 - Google Patents
間葉系幹細胞を区別する方法 Download PDFInfo
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Description
Claims (18)
- 繊維芽細胞から間葉系幹細胞(MSC)を区別する方法であって、
繊維芽細胞からMSCを区別するように、MSC上で発現されるマーカーEphA2を使用して繊維芽細胞からMSCを単離することを含み、
MSCおよび繊維芽細胞は胎盤関連組織に由来する細胞の初代培養からである、方法。 - 胎盤関連組織が、羊膜、絨毛膜板(chorionic disk)、絨毛膜(chorionic membrane)および臍帯からなる群から選択される、請求項1に記載の方法。
- 単離する工程が、抗体ベースの、またはヌクレオチドベースの単離方法によって行われる、請求項1に記載の方法。
- 単離した胎盤関連組織からMSCおよび繊維芽細胞を回収すること;
初代培養を調製するために培養培地においてMSCおよび繊維芽細胞を培養すること
の予備段階をさらに含む、請求項1に記載の方法。 - 抗体ベースの単離方法が抗体ベースの磁気細胞選別または抗体ベースのフローサイトメトリーである、請求項3に記載の方法。
- ヌクレオチドベースの単離方法がヌクレオチドベースのフローサイトメトリーである、請求項3に記載の方法。
- 間葉系幹細胞(MSC)集団の純度を増加させる方法であって、
MSC上で発現されるマーカーEphA2を使用してMSCを単離および回収すること、ここでMSCは胎盤関連組織に由来する細胞の初代培養からであり;および
MSCを培養すること
を含む、方法。 - 胎盤関連組織が、羊膜、絨毛膜板、絨毛膜および臍帯からなる群から選択される、請求項7に記載の方法。
- 単離する工程が、抗体ベースの、またはヌクレオチドベースの単離方法によって行われる、請求項7に記載の方法。
- 胎盤関連組織に由来する細胞がMSCのための培養培地において培養される、請求項7に記載の方法。
- MSC集団の純度が、単離工程後にP0で少なくとも95%である、請求項7に記載の方法。
- 抗体ベースの単離方法が抗体ベースの磁気細胞選別または抗体ベースのフローサイトメトリーである、請求項9に記載の方法。
- ヌクレオチドベースの単離方法がヌクレオチドベースのフローサイトメトリーである、請求項9に記載の方法。
- 炎症環境においてより応答性である間葉系幹細胞(MSC)集団を単離する方法であって、
MSC上で発現されるマーカーEphA2を使用してMSCを単離および回収することを含み、MSCは胎盤関連組織に由来する細胞の初代培養からであり、MSCの集団は炎症環境においてより応答性である、方法。 - 単離する工程が、抗体ベースの、またはヌクレオチドベースの単離方法によって行われる、請求項14に記載の方法。
- MSC集団が、TNF−αシグナル伝達またはTNF−α−依存性シグナル伝達に対してより応答性である、請求項14に記載の方法。
- 抗体ベースの単離方法が抗体ベースの磁気細胞選別または抗体ベースのフローサイトメトリーである、請求項15に記載の方法。
- ヌクレオチドベースの単離方法がヌクレオチドベースのフローサイトメトリーである、請求項15に記載の方法。
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PCT/CN2014/093109 WO2016086403A1 (en) | 2014-12-05 | 2014-12-05 | Method of distinguishing mesenchymal stem cells |
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EP (1) | EP3227682B1 (ja) |
JP (1) | JP6563492B2 (ja) |
KR (1) | KR102045130B1 (ja) |
CN (1) | CN106471371B (ja) |
SG (1) | SG11201703600YA (ja) |
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TWI689589B (zh) * | 2016-08-31 | 2020-04-01 | 宣捷細胞生物製藥股份有限公司 | 區別間質幹細胞的方法及測定間質幹細胞的純度的方法 |
AU2020240049A1 (en) | 2019-03-19 | 2021-11-04 | Figene, Llc | Treatment of chronic obstructive pulmonary disease and lung degeneration using activated fibroblasts and exosome derivatives thereof |
CN111979188A (zh) * | 2020-08-25 | 2020-11-24 | 江苏赛亿细胞技术研究院有限公司 | 一种胎盘间充质干细胞分离培养扩增方法 |
CN118067592A (zh) * | 2024-04-23 | 2024-05-24 | 军科正源(北京)药物研究有限责任公司 | 用于检测间充质干细胞的方法及其应用 |
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WO2004081174A2 (ja) * | 2003-03-10 | 2004-09-23 | Japan Science And Technology Agency | 間葉系幹細胞検出用マーカー及び該マーカーを用いた間葉系幹細胞の識別方法 |
CA2525929A1 (en) * | 2003-05-13 | 2004-11-25 | Chiron Corporation | Methods of modulating metastasis and skeletal related events resulting from metastases |
EP2003196A3 (en) * | 2003-06-09 | 2009-01-07 | The Regents of the University of Michigan | Compositions and methods for treating and diagnosing cancer |
JP4950659B2 (ja) * | 2003-06-27 | 2012-06-13 | エチコン、インコーポレイテッド | 胎盤組織から由来最多分娩後細胞、及びそれらを作成し使用する方法。 |
EP1548100A1 (en) * | 2003-11-11 | 2005-06-29 | DeltaCell B.V. | Inhibition of stem cell differentiation, enhancement of proliferation and selective induction of apoptosis by Wnt factors |
WO2008111161A1 (ja) * | 2007-03-12 | 2008-09-18 | Two Cells Co., Ltd. | 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞 |
JP5282282B2 (ja) * | 2007-09-12 | 2013-09-04 | 公益財団法人ヒューマンサイエンス振興財団 | 内側絨毛膜由来の間葉系幹細胞 |
ITRM20080342A1 (it) * | 2008-06-26 | 2009-12-27 | Univ Degli Studi Udine | Cellule di polpa dentale midollo-simili, metodi per isolamento ed uso. |
EP2362900A2 (en) * | 2008-12-01 | 2011-09-07 | Katholieke Universiteit Leuven, K.U. Leuven R&D | MAINTENANCE/EXPANSION OF HSCs |
JP2010189302A (ja) * | 2009-02-17 | 2010-09-02 | Hiroshima Univ | 病変間葉系幹細胞が関連する疾患の治療剤および間葉系幹細胞の検出マーカーの利用 |
JP2012143158A (ja) * | 2009-05-12 | 2012-08-02 | Hiroshima Univ | 誘導性間葉系幹細胞およびその作製方法 |
US20120040361A1 (en) * | 2010-07-22 | 2012-02-16 | National Tsing Hua University | Methods and compositions for detection of lethal system and uses thereof |
TWI493034B (zh) * | 2011-12-14 | 2015-07-21 | Nat Univ Chung Hsing | Neuronal epithelial cells differentiated by universal stem cells and the medium used and their differentiation methods |
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- 2014-12-05 JP JP2017525390A patent/JP6563492B2/ja active Active
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KR20170071525A (ko) | 2017-06-23 |
EP3227682B1 (en) | 2019-05-01 |
JP2018502556A (ja) | 2018-02-01 |
CN106471371A (zh) | 2017-03-01 |
EP3227682A1 (en) | 2017-10-11 |
CN106471371B (zh) | 2018-05-04 |
WO2016086403A1 (en) | 2016-06-09 |
KR102045130B1 (ko) | 2019-11-14 |
SG11201703600YA (en) | 2017-06-29 |
EP3227682A4 (en) | 2018-06-13 |
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