JP2017184623A - 未分化細胞間における分化傾向の評価方法、分化傾向の評価マーカーとしてのSALL3 mRNA、及び、未分化細胞の分化能力の制御方法 - Google Patents
未分化細胞間における分化傾向の評価方法、分化傾向の評価マーカーとしてのSALL3 mRNA、及び、未分化細胞の分化能力の制御方法 Download PDFInfo
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Abstract
【解決手段】複数の未分化細胞間における分化傾向の評価方法であって、各々の未分化細胞のSALL3 mRNAの発現量を検出する工程と、SALL3 mRNAの発現量が相対的に最も高い細胞が最も外胚葉系に分化する傾向があると評価し、SALL3 mRNAの発現量が相対的に最も低い細胞が最も中胚葉系又は内胚葉系に分化する傾向があると評価する工程と、を有する。
【選択図】図2
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Claims (9)
- 複数の未分化細胞間における分化傾向の評価方法であって、
各々の未分化細胞のSALL3 mRNAの発現量を検出する工程と、
SALL3 mRNAの発現量が相対的に最も高い細胞が最も外胚葉系に分化する傾向があると評価し、SALL3 mRNAの発現量が相対的に最も低い細胞が最も中胚葉系又は内胚葉系に分化する傾向があると評価する工程と、
を有することを特徴とする分化傾向の評価方法。 - 前記SALL3 mRNAの発現量の検出は、PCR法による検出であることを特徴とする請求項1項に記載の分化傾向の評価方法。
- 前記未分化細胞は、ヒトiPS細胞又はヒトES細胞であることを特徴とする請求項1又は2項に記載の分化傾向の評価方法。
- 複数の未分化細胞間における分化傾向の評価マーカーとしてのSALL3 mRNAであって、
SALL3 mRNAの発現量が相対的に最も高い細胞が最も外胚葉系に分化する傾向があると評価し、SALL3 mRNAの発現量が相対的に最も低い細胞が最も中胚葉系又は内胚葉系に分化する傾向があると評価する、ことを特徴とする分化傾向の評価マーカーとしてのSALL3 mRNA。 - 前記未分化細胞は、ヒトiPS細胞又はヒトES細胞であることを特徴とする請求項4項に記載の分化傾向の評価マーカーとしてのSALL3 mRNA。
- 未分化細胞のSALL3 mRNAの発現量を制御することを特徴とする未分化細胞の分化能力の制御方法。
- 前記未分化細胞のSALL3 mRNAの発現量を抑制させることにより、前記未分化細胞が中胚葉系又は内胚葉系に分化する分化能力を有することを特徴とする請求項6に記載の未分化細胞の分化能力の制御方法。
- 前記未分化細胞のSALL3 mRNAの発現量を上昇させることにより、前記未分化細胞が外胚葉系に分化する分化能力を有することを特徴とする請求項6に記載の未分化細胞の分化能力の制御方法。
- 前記未分化細胞は、ヒトiPS細胞又はヒトES細胞であることを特徴とする請求項6乃至8の何れか1項に記載の未分化細胞の分化能力の制御方法。
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PCT/JP2017/013952 WO2017171097A1 (ja) | 2016-04-01 | 2017-04-03 | 未分化細胞間における分化傾向の評価方法、分化傾向の評価マーカーとしてのSALL3 mRNA、及び、未分化細胞の分化能力の制御方法 |
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