JP6426327B2 - 接着状態の細胞培養物の改変方法 - Google Patents

接着状態の細胞培養物の改変方法 Download PDF

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JP6426327B2
JP6426327B2 JP2018521436A JP2018521436A JP6426327B2 JP 6426327 B2 JP6426327 B2 JP 6426327B2 JP 2018521436 A JP2018521436 A JP 2018521436A JP 2018521436 A JP2018521436 A JP 2018521436A JP 6426327 B2 JP6426327 B2 JP 6426327B2
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cells
cell culture
sheet
hours
cell
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JPWO2018084167A1 (ja
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賢二 大山
賢二 大山
涼平 竹内
涼平 竹内
稔和 竹内
稔和 竹内
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Terumo Corp
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2018521436A 2016-11-01 2017-11-01 接着状態の細胞培養物の改変方法 Active JP6426327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016214224 2016-11-01
JP2016214224 2016-11-01
PCT/JP2017/039476 WO2018084167A1 (ja) 2016-11-01 2017-11-01 接着状態の細胞培養物の改変方法

Related Child Applications (1)

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JP2018199652A Division JP2019030322A (ja) 2016-11-01 2018-10-24 接着状態の細胞培養物の改変方法

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JPWO2018084167A1 JPWO2018084167A1 (ja) 2018-11-08
JP6426327B2 true JP6426327B2 (ja) 2018-11-21

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JP2018521436A Active JP6426327B2 (ja) 2016-11-01 2017-11-01 接着状態の細胞培養物の改変方法
JP2018199652A Pending JP2019030322A (ja) 2016-11-01 2018-10-24 接着状態の細胞培養物の改変方法
JP2022076414A Active JP7282232B2 (ja) 2016-11-01 2022-05-06 接着状態の細胞培養物の改変方法

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JP2022076414A Active JP7282232B2 (ja) 2016-11-01 2022-05-06 接着状態の細胞培養物の改変方法

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US (1) US11395863B2 (enExample)
EP (1) EP3406704B1 (enExample)
JP (3) JP6426327B2 (enExample)
CN (1) CN109153963B (enExample)
SG (1) SG11201805456WA (enExample)
WO (1) WO2018084167A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3819373A4 (en) * 2018-07-06 2022-05-18 Myoridge Co. Ltd. CELL LAYER PREPARATION METHOD, HEART MUSCLE LAYER AND KIT FOR MAKING A HEART MUSCLE LAYER
WO2021065989A1 (ja) * 2019-09-30 2021-04-08 テルモ株式会社 Ｃｄ５６陽性細胞の比率を高めるための方法
CN115651906A (zh) * 2022-09-14 2023-01-31 广东省科学院生物与医学工程研究所 一种将免疫引入骨修复的体外仿生组织评价模型及其应用
WO2025183063A1 (ja) * 2024-03-01 2025-09-04 セントラル硝子株式会社 三次元細胞培養体の凍結物、三次元細胞培養体の凍結物を含む容器、細胞凍結キット、凍結保存液、三次元細胞培養体の凍結物の製造方法、三次元細胞培養体の凍結物の使用方法、および凍結保存液の使用方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2354978A1 (en) * 2001-08-02 2003-02-02 Universite Laval Culture medium to improve the purity of myoblast culture
US20030211141A1 (en) * 2001-12-11 2003-11-13 Lebaron Richard G. Genetic and protein manipulation of betaIG-H3 for the treatment and cure of muscular dystrophies
EP2266500B1 (en) 2003-08-01 2015-04-01 CellSeed Inc. Three-dimensional tissue structure
JP2007089442A (ja) * 2005-09-28 2007-04-12 Terumo Corp 骨格筋芽細胞の分離方法
KR101348325B1 (ko) * 2006-01-31 2014-01-08 각고호우징 게이오기주크 줄기세포 및 태아에서 유래된 심근세포 및 예정 심근세포의제조방법
CN101711277B (zh) * 2007-07-31 2018-06-19 第一三共株式会社 心肌细胞的细胞块制备方法和该心肌细胞块的用途
JP5378743B2 (ja) 2008-09-30 2013-12-25 テルモ株式会社 医療用細胞シートの製造方法
JP2010099052A (ja) * 2008-10-27 2010-05-06 Olympus Corp 細胞分離方法
JP5436905B2 (ja) 2009-03-26 2014-03-05 テルモ株式会社 シート状細胞培養物の製造方法
JP5667357B2 (ja) 2009-11-30 2015-02-12 テルモ株式会社 シート状細胞培養物の製造方法
JP5752903B2 (ja) * 2010-08-17 2015-07-22 テルモ株式会社 移植片移送用器具
JPWO2012118099A1 (ja) * 2011-02-28 2014-07-07 学校法人東京女子医科大学 サイトカイン産生細胞シートとその利用方法
CN102191218B (zh) * 2011-03-28 2013-07-24 遵义医学院附属医院 一种完全培养基及人羊膜间充质干细胞的培养方法
US9822342B2 (en) 2013-05-14 2017-11-21 Kyoto University Method of efficiently inducing cardiomyocytes
CN105229145B (zh) * 2013-05-17 2020-08-28 泰尔茂株式会社 片状细胞培养物的制造方法
EP3064578B1 (en) * 2014-02-26 2021-01-13 Terumo Kabushiki Kaisha Method of producing cell population with high target cell purity
JP6495603B2 (ja) * 2014-09-03 2019-04-03 テルモ株式会社 シート状細胞培養物とフィブリンゲルとの積層体の製造方法
JP6494957B2 (ja) * 2014-09-03 2019-04-03 テルモ株式会社 シート状細胞培養物回収システムおよび方法
JP6494958B2 (ja) * 2014-09-03 2019-04-03 テルモ株式会社 シート状細胞培養物回収システムおよび方法
WO2016076368A1 (ja) * 2014-11-12 2016-05-19 テルモ株式会社 心筋細胞シート
WO2017038562A1 (ja) 2015-08-31 2017-03-09 学校法人東京女子医科大学 多能性幹細胞を減少させる方法、多能性幹細胞を減少させた細胞集団の製造方法

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Publication number Publication date
US11395863B2 (en) 2022-07-26
SG11201805456WA (en) 2019-05-30
CN109153963A (zh) 2019-01-04
CN109153963B (zh) 2022-06-24
JP7282232B2 (ja) 2023-05-26
JP2019030322A (ja) 2019-02-28
JPWO2018084167A1 (ja) 2018-11-08
WO2018084167A1 (ja) 2018-05-11
EP3406704A1 (en) 2018-11-28
US20190076576A1 (en) 2019-03-14
JP2022105168A (ja) 2022-07-12
EP3406704A4 (en) 2019-07-17
EP3406704B1 (en) 2021-10-20

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