JP6405308B2 - キメラｋｓａｃタンパク質の発現及び高圧により可溶性タンパク質を製造する方法 - Google Patents

キメラｋｓａｃタンパク質の発現及び高圧により可溶性タンパク質を製造する方法 Download PDF

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JP6405308B2
JP6405308B2 JP2015530070A JP2015530070A JP6405308B2 JP 6405308 B2 JP6405308 B2 JP 6405308B2 JP 2015530070 A JP2015530070 A JP 2015530070A JP 2015530070 A JP2015530070 A JP 2015530070A JP 6405308 B2 JP6405308 B2 JP 6405308B2
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protein
ksac
pressure
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JP2015528466A5 (enExample
JP2015528466A (ja
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ローラン ベルナール フィッシャー
ローラン ベルナール フィッシャー
ニコラ ピエール イヴ カルブレック
ニコラ ピエール イヴ カルブレック
ファビアン リュクス
ファビアン リュクス
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メリアル インコーポレイテッド
メリアル インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/10—Preservation of foods or foodstuffs, in general by treatment with pressure variation, shock, acceleration or shear stress
    • A23B2/103—Preservation of foods or foodstuffs, in general by treatment with pressure variation, shock, acceleration or shear stress using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/002—Protozoa antigens
    • A61K39/008—Leishmania antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/0225—Spirochetes, e.g. Treponema, Leptospira, Borrelia
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/0241—Mollicutes, e.g. Mycoplasma, Erysipelothrix
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/099—Bordetella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01041—Sterol 24-C-methyltransferasee (2.1.1.41)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0031—Serum-free culture media
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Food Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2015530070A 2012-08-30 2013-08-30 キメラｋｓａｃタンパク質の発現及び高圧により可溶性タンパク質を製造する方法 Expired - Fee Related JP6405308B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261694968P 2012-08-30 2012-08-30
US61/694,968 2012-08-30
US201361830425P 2013-06-03 2013-06-03
US61/830,425 2013-06-03
PCT/US2013/057430 WO2014036349A1 (en) 2012-08-30 2013-08-30 Expression of chimeric ksac protein and method of producing soluble proteins by high pressure

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JP2015528466A JP2015528466A (ja) 2015-09-28
JP2015528466A5 JP2015528466A5 (enExample) 2016-08-12
JP6405308B2 true JP6405308B2 (ja) 2018-10-17

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JP2015530070A Expired - Fee Related JP6405308B2 (ja) 2012-08-30 2013-08-30 キメラｋｓａｃタンパク質の発現及び高圧により可溶性タンパク質を製造する方法
JP2015530066A Active JP6438396B2 (ja) 2012-08-30 2013-08-30 不活化ワクチンを作製するための、ならびに組換えタンパク質をリフォールディング／可溶化させるための高圧デバイスおよび方法

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KR (1) KR102208844B1 (enExample)
CN (3) CN104717976A (enExample)
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MX2015002701A (es) 2015-12-01
CA2883303A1 (en) 2014-03-06
US20170081372A1 (en) 2017-03-23
MD4608B1 (ro) 2018-12-31
KR102208844B1 (ko) 2021-01-27
AU2013308627B2 (en) 2016-10-27
MX2015002699A (es) 2015-12-01
AU2017200301A1 (en) 2017-02-16
PH12015500700A1 (en) 2015-05-25
AU2017201724A1 (en) 2017-03-30
BR112015004463A2 (pt) 2017-08-08
CN104768577A (zh) 2015-07-08
MX381717B (es) 2025-03-13
AU2013308623B2 (en) 2017-03-02
JP6438396B2 (ja) 2018-12-12
NZ706288A (en) 2016-06-24
ES2744709T3 (es) 2020-02-26
EA201500275A1 (ru) 2015-07-30
CN104768577B (zh) 2020-06-09
HK1211873A1 (en) 2016-06-03
AU2017201724B2 (en) 2019-01-17
WO2014036345A3 (en) 2014-06-26
EP2890393A2 (en) 2015-07-08
CA2883296A1 (en) 2014-03-06
MD20150031A2 (ro) 2015-10-31
US20140065187A1 (en) 2014-03-06
EA031988B1 (ru) 2019-03-29
US9315553B2 (en) 2016-04-19
NZ715459A (en) 2017-05-26
US10364274B2 (en) 2019-07-30
PH12015500700B1 (en) 2018-11-28
WO2014036345A2 (en) 2014-03-06
EP2890392B1 (en) 2019-04-17
BR112015004463B8 (pt) 2022-06-28
AU2013308627A1 (en) 2015-04-09
MD4608C1 (ro) 2019-07-31
BR112015004463B1 (pt) 2021-08-24
HK1211874A1 (zh) 2016-06-03
EP2890392A1 (en) 2015-07-08
RU2647568C2 (ru) 2018-03-16
KR20150063403A (ko) 2015-06-09
RU2015111255A (ru) 2016-10-20
NZ705790A (en) 2016-06-24
CN108578679A (zh) 2018-09-28
AU2013308623A1 (en) 2015-04-02
US20140072594A1 (en) 2014-03-13
WO2014036349A1 (en) 2014-03-06
MX2021004478A (es) 2021-06-04
MX358782B (es) 2018-09-04
CN104717976A (zh) 2015-06-17
AP2015008331A0 (en) 2015-03-31
JP2015528466A (ja) 2015-09-28
JP2015529462A (ja) 2015-10-08
US9169302B2 (en) 2015-10-27

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