JP6381518B2 - 改良された脂肪酸誘導体産生方法 - Google Patents

改良された脂肪酸誘導体産生方法 Download PDF

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JP6381518B2
JP6381518B2 JP2015504687A JP2015504687A JP6381518B2 JP 6381518 B2 JP6381518 B2 JP 6381518B2 JP 2015504687 A JP2015504687 A JP 2015504687A JP 2015504687 A JP2015504687 A JP 2015504687A JP 6381518 B2 JP6381518 B2 JP 6381518B2
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fatty
fatty acid
host cell
polypeptide
recombinant host
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JP2015512644A (ja
JP2015512644A5 (enExample
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デレク エル． グリーンフィールド
デレク エル． グリーンフィールド
アンドレアス ダブリュ． シャーマー
アンドレアス ダブリュ． シャーマー
エリザベス ジェイ． クラーク
エリザベス ジェイ． クラーク
イーライ エス． グローバン
イーライ エス． グローバン
コスタ バーナード エム． ダ
コスタ バーナード エム． ダ
チーハオ フ
チーハオ フ
ケビン ホールデン
ケビン ホールデン
ノア ヘルマン
ノア ヘルマン
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アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
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    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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JP2015504687A 2012-04-02 2013-04-02 改良された脂肪酸誘導体産生方法 Expired - Fee Related JP6381518B2 (ja)

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US201261619324P 2012-04-02 2012-04-02
US61/619,324 2012-04-02
PCT/US2013/035037 WO2013152051A2 (en) 2012-04-02 2013-04-02 Improved production of fatty acid derivatives

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US (4) US20150064782A1 (enExample)
EP (2) EP2834352B1 (enExample)
JP (3) JP6381518B2 (enExample)
KR (2) KR102353113B1 (enExample)
CN (2) CN104395464B (enExample)
AU (1) AU2013243601B2 (enExample)
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Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2883968C (en) 2012-04-02 2022-08-23 REG Life Sciences, LLC Improved production of fatty acid derivatives
CN109182238A (zh) 2012-08-10 2019-01-11 嘉吉有限公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
EP2970988A4 (en) * 2013-03-15 2016-12-07 Cargill Inc CONTROL OF PHASES OF GROWTH INDUCTION PRODUCTION
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
WO2015054138A1 (en) * 2013-10-10 2015-04-16 William Marsh Rice University Improved fatty acid productivity
WO2015095240A2 (en) * 2013-12-20 2015-06-25 The Regents Of The University Of California Biosynthesis of 1-undecence and related terminal olefins
WO2015200335A1 (en) * 2014-06-23 2015-12-30 Joule Unlimited Technologies, Inc. Engineered photosynthetic microbes and recombinant synthesis of carbon-based products
BR112017001105A2 (pt) 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
JP6934303B2 (ja) 2017-02-08 2021-09-15 花王株式会社 脂肪族アルコールの生産方法
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
EP3775241A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Methods for controlling oxygen concentration during aerobic biosynthesis
US10975363B2 (en) 2018-03-30 2021-04-13 Inv Nylon Chemicals Americas, Llc Materials and methods for biosynthetic manufacture and utilization of synthetic polypeptides, and products therefrom
EP3775240A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation
EP3775242A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited High hydrogen utilization and gas recycle
CN112004934B (zh) 2018-03-30 2024-02-23 英威达纺织（英国）有限公司 用于生物合成制造碳基化学品的材料和方法
US12060596B2 (en) 2018-05-02 2024-08-13 Inv Nylon Chemicals Americas, Llc Materials and methods for controlling limitation conditions in product biosynthesis for non-PHB generating species of the genera Ralstonia or Cupriavidus and organisms related thereto
WO2019213017A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Materials and methods for controlling oxidation and reduction in biosynthetic pathways of species of the genera ralstonia and cupriavidus and organisms related thereto
US11053287B2 (en) 2018-05-02 2021-07-06 Inv Nylon Chemicals Americas, Llc Materials and methods for differential biosynthesis in species of the genera Ralstonia and Cupriavidus and organisms related thereto
US11098381B2 (en) 2018-05-02 2021-08-24 Inv Nylon Chemicals Americas, Llc Materials and methods for controlling regulation in biosynthesis in species of the genera Ralstonia or Cupriavidus and organisms related thereto
US11999943B2 (en) 2018-05-02 2024-06-04 Inv Nylon Chemicals Americas, Llc Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
KR102117828B1 (ko) 2018-07-19 2020-06-02 서울대학교산학협력단 레귤론 변이를 통한 지방산 및 지방산 유도체 생산에서 불포화도 감소
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
WO2020181091A1 (en) 2019-03-05 2020-09-10 Bp Corporation North America Inc. Fatty acyl-acp reductases and their uses
US20250115892A1 (en) * 2021-12-06 2025-04-10 Cnpem - Centro Nacional De Pesquisa Em Energia E Materiais Chimeric enzyme and method for producing terminal alkenes
CN114634917A (zh) * 2022-03-25 2022-06-17 湖南农业大学 accD突变蛋白在提高植物种子脂肪酸含量中的应用
EP4630526A1 (en) 2022-12-05 2025-10-15 The Procter & Gamble Company Fabric and home care composition comprising a polyalkylenecarbonate compound
TW202607136A (zh) * 2024-06-27 2026-02-16 德商巴地斯顏料化工廠 自甲醇製備c6-c26脂肪醇

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
EP0861321B1 (en) * 1995-10-13 2006-05-31 President And Fellows Of Harvard College Phosphopantetheinyl transferases and uses thereof
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
CN1154745C (zh) * 1999-11-09 2004-06-23 浙江省农业科学院 利用反义基因调控籽粒油脂含量的方法
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
US20100242345A1 (en) * 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
ES2528482T3 (es) 2006-05-19 2015-02-10 Ls9, Inc. Producción de ácidos grasos y derivados de los mismos
WO2008119082A2 (en) 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
US8753840B2 (en) * 2006-10-20 2014-06-17 Arizona Board Of Regents On Behalf Of Arizona State University Modified cyanobacteria
US8110093B2 (en) 2007-03-14 2012-02-07 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
KR101730290B1 (ko) 2007-05-11 2017-04-25 토마스 제퍼슨 유니버시티 신경변성 질환 및 장애의 치료 및 예방 방법
KR101862691B1 (ko) 2007-05-22 2018-05-31 알이지 라이프 사이언시스, 엘엘씨 탄화수소-생산 유전자와 이들의 이용 방법
WO2009002480A2 (en) 2007-06-26 2008-12-31 Danisco Us, Inc., Genencor Division Acyl transferase having altered substrate specificity
US7897369B2 (en) 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
US20110111458A1 (en) 2008-03-18 2011-05-12 Kyowa Hakko Kirin Co., Ltd. Industrially useful microorganism
CA3101888A1 (en) 2008-05-16 2009-11-19 REG Life Sciences, LLC Methods and compositions for producing hydrocarbons
US8097439B2 (en) 2008-10-07 2012-01-17 Ls9, Inc. Methods and compositions for producing fatty aldehydes
CN105154380B (zh) 2008-10-28 2018-11-09 Reg生命科学有限责任公司 用于产生脂肪醇的方法和组合物
CA2747516C (en) 2008-12-23 2019-11-05 Ls9, Inc. Methods and compositions related to thioesterase enzymes
EP2425007A4 (en) * 2009-04-27 2013-01-02 Ls9 Inc PREPARATION OF FATTY ACID ESTERS
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
MX2012003472A (es) 2009-09-25 2012-05-22 Ls9 Inc Produccion de derivados de acido graso.
US20110151526A1 (en) * 2009-12-22 2011-06-23 Charles Winston Saunders Branched-Chain Fatty Acids And Biological Production Thereof
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20120172281A1 (en) * 2010-07-15 2012-07-05 Jeffrey John Scheibel Detergent compositions comprising microbially produced fatty alcohols and derivatives thereof
US10138504B2 (en) 2010-08-06 2018-11-27 Lallemand Hungary Liquidity Management Llc Production of malonyl-CoA derived products via anaerobic pathways
US8372610B2 (en) * 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
BR112014002624A8 (pt) * 2011-08-03 2017-07-04 Ls9 Inc Microorganismo recombinante e cultura de microorganismo recombinante
US8790901B2 (en) 2011-12-14 2014-07-29 Pronutria, Inc. Microorganisms and methods for producing unsaturated fatty acids
WO2013152052A2 (en) * 2012-04-02 2013-10-10 Ls9, Inc. Novel car enzymes and improved production of fatty alcohols
CA2883968C (en) 2012-04-02 2022-08-23 REG Life Sciences, LLC Improved production of fatty acid derivatives
CN104955944B (zh) * 2012-09-14 2021-03-09 基因组股份公司 具有改善的酯合酶特性的酶变体
WO2014093505A2 (en) * 2012-12-12 2014-06-19 Ls9, Inc. Acp-mediated production of fatty acid derivatives

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