KR102353113B1 - 지방산 유도체들의 개선된 생산 - Google Patents

지방산 유도체들의 개선된 생산 Download PDF

Info

Publication number
KR102353113B1
KR102353113B1 KR1020207015194A KR20207015194A KR102353113B1 KR 102353113 B1 KR102353113 B1 KR 102353113B1 KR 1020207015194 A KR1020207015194 A KR 1020207015194A KR 20207015194 A KR20207015194 A KR 20207015194A KR 102353113 B1 KR102353113 B1 KR 102353113B1
Authority
KR
South Korea
Prior art keywords
leu
ala
gly
glu
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020207015194A
Other languages
English (en)
Korean (ko)
Other versions
KR20200062388A (ko
Inventor
데렉 엘. 그린필드
안드레아스 더블유. 셔머
엘리자베스 제이. 클라크
엘리 에스. 그로반
베르나르도 엠. 다 코스타
지하오 흐
케빈 홀덴
노아 헬만
Original Assignee
게노마티카 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 게노마티카 인코포레이티드 filed Critical 게노마티카 인코포레이티드
Publication of KR20200062388A publication Critical patent/KR20200062388A/ko
Application granted granted Critical
Publication of KR102353113B1 publication Critical patent/KR102353113B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/011—3-Oxoacyl-[acyl-carrier-protein] reductase (1.1.1.100)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01009—Enoyl-[acyl-carrier-protein] reductase (NADH) (1.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/0101—Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific)(1.3.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01039—[Acyl-carrier-protein] S-malonyltransferase (2.3.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01179—Beta-ketoacyl-acyl-carrier-protein synthase II (2.3.1.179)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0118—Beta-ketoacyl-acyl-carrier-protein synthase III (2.3.1.180)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08007—Holo-[acyl-carrier-protein] synthase (2.7.8.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01059—3-Hydroxyacyl-[acyl-carrier-protein] dehydratase (4.2.1.59)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/03—Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03014—Trans-2-decenoyl-[acyl-carrier-protein] isomerase (5.3.3.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020207015194A 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산 Active KR102353113B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261619324P 2012-04-02 2012-04-02
US61/619,324 2012-04-02
PCT/US2013/035037 WO2013152051A2 (en) 2012-04-02 2013-04-02 Improved production of fatty acid derivatives
KR1020147030467A KR102166271B1 (ko) 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020147030467A Division KR102166271B1 (ko) 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산

Publications (2)

Publication Number Publication Date
KR20200062388A KR20200062388A (ko) 2020-06-03
KR102353113B1 true KR102353113B1 (ko) 2022-01-19

Family

ID=48087775

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020207015194A Active KR102353113B1 (ko) 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산
KR1020147030467A Expired - Fee Related KR102166271B1 (ko) 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020147030467A Expired - Fee Related KR102166271B1 (ko) 2012-04-02 2013-04-02 지방산 유도체들의 개선된 생산

Country Status (12)

Country Link
US (4) US20150064782A1 (enExample)
EP (2) EP2834352B1 (enExample)
JP (3) JP6381518B2 (enExample)
KR (2) KR102353113B1 (enExample)
CN (2) CN104395464B (enExample)
AU (1) AU2013243601B2 (enExample)
BR (3) BR122019026127B1 (enExample)
CA (2) CA2883968C (enExample)
CO (1) CO7170149A2 (enExample)
MX (2) MX379251B (enExample)
MY (1) MY181688A (enExample)
WO (1) WO2013152051A2 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2883968C (en) 2012-04-02 2022-08-23 REG Life Sciences, LLC Improved production of fatty acid derivatives
CN109182238A (zh) 2012-08-10 2019-01-11 嘉吉有限公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
EP2970988A4 (en) * 2013-03-15 2016-12-07 Cargill Inc CONTROL OF PHASES OF GROWTH INDUCTION PRODUCTION
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
WO2015054138A1 (en) * 2013-10-10 2015-04-16 William Marsh Rice University Improved fatty acid productivity
WO2015095240A2 (en) * 2013-12-20 2015-06-25 The Regents Of The University Of California Biosynthesis of 1-undecence and related terminal olefins
WO2015200335A1 (en) * 2014-06-23 2015-12-30 Joule Unlimited Technologies, Inc. Engineered photosynthetic microbes and recombinant synthesis of carbon-based products
BR112017001105A2 (pt) 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
JP6934303B2 (ja) 2017-02-08 2021-09-15 花王株式会社 脂肪族アルコールの生産方法
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
EP3775241A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Methods for controlling oxygen concentration during aerobic biosynthesis
US10975363B2 (en) 2018-03-30 2021-04-13 Inv Nylon Chemicals Americas, Llc Materials and methods for biosynthetic manufacture and utilization of synthetic polypeptides, and products therefrom
EP3775240A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation
EP3775242A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited High hydrogen utilization and gas recycle
CN112004934B (zh) 2018-03-30 2024-02-23 英威达纺织（英国）有限公司 用于生物合成制造碳基化学品的材料和方法
US12060596B2 (en) 2018-05-02 2024-08-13 Inv Nylon Chemicals Americas, Llc Materials and methods for controlling limitation conditions in product biosynthesis for non-PHB generating species of the genera Ralstonia or Cupriavidus and organisms related thereto
WO2019213017A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Materials and methods for controlling oxidation and reduction in biosynthetic pathways of species of the genera ralstonia and cupriavidus and organisms related thereto
US11053287B2 (en) 2018-05-02 2021-07-06 Inv Nylon Chemicals Americas, Llc Materials and methods for differential biosynthesis in species of the genera Ralstonia and Cupriavidus and organisms related thereto
US11098381B2 (en) 2018-05-02 2021-08-24 Inv Nylon Chemicals Americas, Llc Materials and methods for controlling regulation in biosynthesis in species of the genera Ralstonia or Cupriavidus and organisms related thereto
US11999943B2 (en) 2018-05-02 2024-06-04 Inv Nylon Chemicals Americas, Llc Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
KR102117828B1 (ko) 2018-07-19 2020-06-02 서울대학교산학협력단 레귤론 변이를 통한 지방산 및 지방산 유도체 생산에서 불포화도 감소
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
WO2020181091A1 (en) 2019-03-05 2020-09-10 Bp Corporation North America Inc. Fatty acyl-acp reductases and their uses
US20250115892A1 (en) * 2021-12-06 2025-04-10 Cnpem - Centro Nacional De Pesquisa Em Energia E Materiais Chimeric enzyme and method for producing terminal alkenes
CN114634917A (zh) * 2022-03-25 2022-06-17 湖南农业大学 accD突变蛋白在提高植物种子脂肪酸含量中的应用
EP4630526A1 (en) 2022-12-05 2025-10-15 The Procter & Gamble Company Fabric and home care composition comprising a polyalkylenecarbonate compound
TW202607136A (zh) * 2024-06-27 2026-02-16 德商巴地斯顏料化工廠 自甲醇製備c6-c26脂肪醇

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
WO2012009660A2 (en) * 2010-07-15 2012-01-19 The Procter & Gamble Company Detergent compositions comprising microbially produced fatty alcohols and derivatives thereof

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
EP0861321B1 (en) * 1995-10-13 2006-05-31 President And Fellows Of Harvard College Phosphopantetheinyl transferases and uses thereof
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
CN1154745C (zh) * 1999-11-09 2004-06-23 浙江省农业科学院 利用反义基因调控籽粒油脂含量的方法
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
ES2528482T3 (es) 2006-05-19 2015-02-10 Ls9, Inc. Producción de ácidos grasos y derivados de los mismos
WO2008119082A2 (en) 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
US8753840B2 (en) * 2006-10-20 2014-06-17 Arizona Board Of Regents On Behalf Of Arizona State University Modified cyanobacteria
US8110093B2 (en) 2007-03-14 2012-02-07 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
KR101730290B1 (ko) 2007-05-11 2017-04-25 토마스 제퍼슨 유니버시티 신경변성 질환 및 장애의 치료 및 예방 방법
KR101862691B1 (ko) 2007-05-22 2018-05-31 알이지 라이프 사이언시스, 엘엘씨 탄화수소-생산 유전자와 이들의 이용 방법
WO2009002480A2 (en) 2007-06-26 2008-12-31 Danisco Us, Inc., Genencor Division Acyl transferase having altered substrate specificity
US7897369B2 (en) 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
US20110111458A1 (en) 2008-03-18 2011-05-12 Kyowa Hakko Kirin Co., Ltd. Industrially useful microorganism
CA3101888A1 (en) 2008-05-16 2009-11-19 REG Life Sciences, LLC Methods and compositions for producing hydrocarbons
US8097439B2 (en) 2008-10-07 2012-01-17 Ls9, Inc. Methods and compositions for producing fatty aldehydes
CN105154380B (zh) 2008-10-28 2018-11-09 Reg生命科学有限责任公司 用于产生脂肪醇的方法和组合物
CA2747516C (en) 2008-12-23 2019-11-05 Ls9, Inc. Methods and compositions related to thioesterase enzymes
EP2425007A4 (en) * 2009-04-27 2013-01-02 Ls9 Inc PREPARATION OF FATTY ACID ESTERS
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
MX2012003472A (es) 2009-09-25 2012-05-22 Ls9 Inc Produccion de derivados de acido graso.
US20110151526A1 (en) * 2009-12-22 2011-06-23 Charles Winston Saunders Branched-Chain Fatty Acids And Biological Production Thereof
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US10138504B2 (en) 2010-08-06 2018-11-27 Lallemand Hungary Liquidity Management Llc Production of malonyl-CoA derived products via anaerobic pathways
US8372610B2 (en) * 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
BR112014002624A8 (pt) * 2011-08-03 2017-07-04 Ls9 Inc Microorganismo recombinante e cultura de microorganismo recombinante
US8790901B2 (en) 2011-12-14 2014-07-29 Pronutria, Inc. Microorganisms and methods for producing unsaturated fatty acids
WO2013152052A2 (en) * 2012-04-02 2013-10-10 Ls9, Inc. Novel car enzymes and improved production of fatty alcohols
CA2883968C (en) 2012-04-02 2022-08-23 REG Life Sciences, LLC Improved production of fatty acid derivatives
CN104955944B (zh) * 2012-09-14 2021-03-09 基因组股份公司 具有改善的酯合酶特性的酶变体
WO2014093505A2 (en) * 2012-12-12 2014-06-19 Ls9, Inc. Acp-mediated production of fatty acid derivatives

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
WO2012009660A2 (en) * 2010-07-15 2012-01-19 The Procter & Gamble Company Detergent compositions comprising microbially produced fatty alcohols and derivatives thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biochemistry (1998) 37:1585-1595*
Current Opinion in Biotechnology (2011) 22:388-393
Journal of Bacteriology (1996) 178(19):5660-5667
Metabolic Engineering (2011) 13:28-37*
Methods in Enzymology (2009) 459:395-433
Plant Physiology (2001) 127:222-229
Trends in Biotechnology (2012.12.) 30(12):659-667

Also Published As

Publication number Publication date
US20240150774A1 (en) 2024-05-09
JP2015512644A (ja) 2015-04-30
CN104395464A (zh) 2015-03-04
CA2883968A1 (en) 2013-10-10
CO7170149A2 (es) 2015-01-28
AU2013243601A1 (en) 2014-10-30
JP2018134085A (ja) 2018-08-30
EP2834352B1 (en) 2020-01-08
KR20200062388A (ko) 2020-06-03
MX2014011905A (es) 2015-05-08
KR102166271B1 (ko) 2020-10-16
MX366939B (es) 2019-07-31
US20180010137A1 (en) 2018-01-11
CA2883968C (en) 2022-08-23
BR112014024675B1 (pt) 2021-12-07
US20210348173A1 (en) 2021-11-11
CN104395464B (zh) 2022-03-04
KR20150032930A (ko) 2015-03-31
MX2019004568A (es) 2019-08-05
JP6738852B2 (ja) 2020-08-12
BR122019026142B1 (pt) 2021-12-14
JP7366836B2 (ja) 2023-10-23
EP3674400A1 (en) 2020-07-01
BR112014024675A8 (pt) 2018-04-24
EP2834352A2 (en) 2015-02-11
JP6381518B2 (ja) 2018-08-29
WO2013152051A2 (en) 2013-10-10
WO2013152051A3 (en) 2013-12-12
CA3162104A1 (en) 2013-10-10
MX379251B (es) 2025-03-11
US11060099B2 (en) 2021-07-13
JP2020120685A (ja) 2020-08-13
CN114456996A (zh) 2022-05-10
BR122019026127B1 (pt) 2021-12-21
MY181688A (en) 2021-01-01
US20150064782A1 (en) 2015-03-05
AU2013243601B2 (en) 2018-06-14

Similar Documents

Publication Publication Date Title
KR102166271B1 (ko) 지방산 유도체들의 개선된 생산
KR102282677B1 (ko) Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
EP3103867B1 (en) Acyl-acp reductase with improved properties
US12180515B2 (en) Omega-hydroxylase-related fusion polypeptide variants with improved properties
AU2015277261B2 (en) Omega-hydroxylase-related fusion polypeptides with improved properties

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20200527

Application number text: 1020147030467

Filing date: 20141029

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20200624

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20200902

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20210415

Patent event code: PE09021S01D

N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20210517

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20211014

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20220114

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20220117

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20241216

Start annual number: 4

End annual number: 4