JP6282467B2 - タンパク質を生産するための方法 - Google Patents

タンパク質を生産するための方法 Download PDF

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Publication number
JP6282467B2
JP6282467B2 JP2013532180A JP2013532180A JP6282467B2 JP 6282467 B2 JP6282467 B2 JP 6282467B2 JP 2013532180 A JP2013532180 A JP 2013532180A JP 2013532180 A JP2013532180 A JP 2013532180A JP 6282467 B2 JP6282467 B2 JP 6282467B2
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culture
protein
cells
suspension
cell culture
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Japanese (ja)
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JP2013538588A (ja
JP2013538588A5 (enExample
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ヤルノ・ロビン
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ノヴォ・ノルディスク・ヘルス・ケア・アーゲー
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2013532180A 2010-10-05 2011-10-05 タンパク質を生産するための方法 Active JP6282467B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10186545.9 2010-10-05
EP10186545 2010-10-05
US39271310P 2010-10-13 2010-10-13
US61/392,713 2010-10-13
PCT/EP2011/067372 WO2012045769A1 (en) 2010-10-05 2011-10-05 Process for protein production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016153606A Division JP2016187360A (ja) 2010-10-05 2016-08-04 タンパク質を生産するための方法

Publications (3)

Publication Number Publication Date
JP2013538588A JP2013538588A (ja) 2013-10-17
JP2013538588A5 JP2013538588A5 (enExample) 2014-11-20
JP6282467B2 true JP6282467B2 (ja) 2018-02-21

Family

ID=43735957

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013532180A Active JP6282467B2 (ja) 2010-10-05 2011-10-05 タンパク質を生産するための方法
JP2016153606A Withdrawn JP2016187360A (ja) 2010-10-05 2016-08-04 タンパク質を生産するための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016153606A Withdrawn JP2016187360A (ja) 2010-10-05 2016-08-04 タンパク質を生産するための方法

Country Status (7)

Country Link
US (1) US10138290B2 (enExample)
EP (2) EP2625262B1 (enExample)
JP (2) JP6282467B2 (enExample)
CN (2) CN107190034A (enExample)
ES (1) ES2556454T3 (enExample)
PL (1) PL2625262T3 (enExample)
WO (1) WO2012045769A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015526094A (ja) * 2012-08-20 2015-09-10 テルモ ビーシーティー、インコーポレーテッド 細胞成長チャンバーを循環する流体の成分を濃縮する方法
SG11201601182QA (en) 2013-09-30 2016-04-28 Crucell Holland Bv Method for the clarification of high density crude cell culture harvest
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
CA3032099A1 (en) * 2016-07-25 2018-02-01 Repligen Corporation Alternating tangential flow rapid harvesting
EP3700505A4 (en) * 2017-10-26 2021-01-06 Repligen Corporation MICRO-ALTERNATIVE TANGENTIAL FLOW INFUSION FILTER, PROCESSING CONTAINER, AND METHODS OF USE THEREOF
TWI846694B (zh) 2018-05-04 2024-07-01 美商健臻公司 具有過濾系統的灌注式生物反應器
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用
CA3144125A1 (en) * 2019-07-16 2021-01-21 Haihong Sun Cell culture method based on high-density and continuous inoculation and use thereof

Family Cites Families (30)

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WO1986006408A1 (en) 1985-04-22 1986-11-06 Genetics Institute, Inc. HIGH YIElD PRODUCTION OF ACTIVE FACTOR IX
ATE87663T1 (de) 1986-01-03 1993-04-15 Genetics Inst Verfahren zur herstellung von faktor-viii:c-typ- proteinen.
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
US4956080A (en) * 1987-08-03 1990-09-11 Microlift Systems, Incorporated High pressure oxygen-saturated water treatment apparatus
JPH066054B2 (ja) 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
JPH084495B2 (ja) 1988-08-19 1996-01-24 帝人株式会社 接着性動物細胞の培養方法
WO1990002175A1 (en) 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US6268487B1 (en) * 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US5851800A (en) 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
DK1356073T3 (da) 2000-10-20 2010-04-06 Bioneer As Fermenteringsfremgangsmåde til fremstilling af heterologe genprodukter i mælkesyrebakterier
US6960657B2 (en) 2001-11-02 2005-11-01 Novo Nordisk Healthcare A/G Human coagulation factor VII polypeptides
JP2005512524A (ja) * 2001-11-02 2005-05-12 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト ヒト凝固第ｖｉｉ因子ポリペプチド
ES2363205T3 (es) 2002-06-21 2011-07-26 Novo Nordisk Health Care Ag Glicoformas del factor vii pegilado.
US7351688B2 (en) 2003-02-05 2008-04-01 The Research Foundation Of State University Of New York Compositions and methods for less immunogenic protein formulations
DE602004008451T2 (de) 2003-05-01 2008-05-15 Dsm Ip Assets B.V. Verfahren zur herstellung von biologischen substanzen durch perfusionskultivierung von suspendierten tierischen zellen
NZ542585A (en) 2003-05-09 2007-11-30 Crucell Holland Bv Cultures of PER.C6-immortalized cells and processes for culturing the same to increase product yields therefrom
ES2565077T3 (es) * 2003-10-10 2016-03-31 Novo Nordisk Health Care Ag Método para producción en gran escala de un polipéptido en células eucariotas
PL1720972T3 (pl) 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
RU2396347C2 (ru) 2004-08-17 2010-08-10 Ксл Бехринг Гмбх Модифицированные витамин к-зависимые полипептиды
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
JP2009535019A (ja) 2006-04-21 2009-10-01 バイエル・ヘルスケア・エルエルシー 灌流培養への哺乳動物細胞中の抗アポトーシス遺伝子の応用
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
FI2343362T4 (fi) * 2006-07-14 2024-07-04 Patheon Holdings I B V Parannettu menetelmä solujen viljelyä varten
EP1988101A1 (en) 2007-05-04 2008-11-05 Novo Nordisk A/S Improvement of factor VIII polypeptide titers in cell cultures
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
JP2009045019A (ja) 2007-08-21 2009-03-05 Toray Ind Inc タンパク質の製造方法
EP2268807A2 (en) * 2008-04-21 2011-01-05 Novo Nordisk A/S Hyperglycosylated human coagulation factor ix
WO2010003759A2 (en) 2008-06-17 2010-01-14 Dsm Ip Assets B.V. Cell culturing method

Also Published As

Publication number Publication date
PL2625262T3 (pl) 2016-05-31
JP2013538588A (ja) 2013-10-17
EP2957628A1 (en) 2015-12-23
EP2625262A1 (en) 2013-08-14
EP2625262B1 (en) 2015-09-23
ES2556454T3 (es) 2016-01-18
JP2016187360A (ja) 2016-11-04
US20130244283A1 (en) 2013-09-19
US10138290B2 (en) 2018-11-27
CN103154233A (zh) 2013-06-12
WO2012045769A1 (en) 2012-04-12
CN107190034A (zh) 2017-09-22

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