JP6053198B2 - βグルカンの製造方法 - Google Patents
βグルカンの製造方法 Download PDFInfo
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- JP6053198B2 JP6053198B2 JP2014525745A JP2014525745A JP6053198B2 JP 6053198 B2 JP6053198 B2 JP 6053198B2 JP 2014525745 A JP2014525745 A JP 2014525745A JP 2014525745 A JP2014525745 A JP 2014525745A JP 6053198 B2 JP6053198 B2 JP 6053198B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Animal Behavior & Ethology (AREA)
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- Polymers & Plastics (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
下記表1に示す組成の培地1000mLを仕込んだ5000mLジャーファーメンターに、バシラス属乳酸生産菌であるBacillus coagulans IFO12714を植菌し、50℃、通気量1.0vvm、撹拌回転数400rpmで8時間好気培養した。
土壌、食品工場、澱粉工場、植物表面等から採取した微生物サンプルをポテトデキストロース寒天培地に塗布し、得られた多くの微生物コロニーの中から目視により黒酵母に近いと思われる性状(粘性のある白色又はややピンク色のコロニーであり、放置すると茶色〜黒色に変化する)のものを選択し分離した。
試験例2で得られたアウレオバシジウム・プルランス(Aureobasidium pullulans MR01)菌体をPBS(リン酸緩衝生理食塩水)に懸濁して1000CFU/mL程度の溶液とし、その0.2mLを下記表4に示す組成の寒天培地のプレートに塗布して、紫外線ランプによる紫外線照射処理を行った。照射時間は、2〜5分間とした。そのまま、寒天プレート上で24℃、6日間培養したところ、米糠培地におけるより小さい白色のコロニーが形成されるのが観察された。このようなコロニーを大きいものから順に100株選別した。
下記表10に示す組成の培地100mLを300mLの三角フラスコに仕込み、MRB001株を植菌して、撹拌回転数150rpmで3日間、振盪培養を行い、前培養とした。
下記表13に示す組成の培地100mLを300mLの三角フラスコに仕込み、MRB001株を植菌して、撹拌回転数150rpmで3日間、振盪培養を行い、前培養とした。
下記表15に示す組成の培地100mLを300mLの三角フラスコに仕込み、MRB001株を植菌して、撹拌回転数150rpmで3日間、振盪培養を行い、前培養とした。
Claims (4)
- 乳酸生産菌の菌体及び/又はその派生物を栄養原として黒酵母(Aureobasidium pullulans)を培養して、当該黒酵母にβグルカンを生産させる工程を有することを特徴とするβグルカンの製造方法であり、
前記黒酵母は、乳酸生産菌の菌体及び/又はその派生物を唯一の窒素源として生育可能なものであるβグルカンの製造方法。 - 前記黒酵母は、アウレオバシジウム・プルランス MRB001(Aureobasidium pullulans MRB001)株(受託番号:NITE BP−1386)である請求項1記載のβグルカンの製造方法。
- 前記乳酸生産菌は、バシラス・コアギュランス(Bacillus coagulans)である請求項1記載のβグルカンの製造方法。
- アウレオバシジウム・プルランス MRB001(Aureobasidium pullulans MRB001)株(受託番号:NITE BP−1386)である黒酵母。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012158805 | 2012-07-17 | ||
JP2012158805 | 2012-07-17 | ||
PCT/JP2013/064862 WO2014013793A1 (ja) | 2012-07-17 | 2013-05-29 | βグルカンの製造方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2014013793A1 JPWO2014013793A1 (ja) | 2016-06-30 |
JP6053198B2 true JP6053198B2 (ja) | 2016-12-27 |
Family
ID=49948629
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014525745A Expired - Fee Related JP6053198B2 (ja) | 2012-07-17 | 2013-05-29 | βグルカンの製造方法 |
Country Status (3)
Country | Link |
---|---|
US (1) | US9896706B2 (ja) |
JP (1) | JP6053198B2 (ja) |
WO (1) | WO2014013793A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7327023B2 (ja) | 2019-09-12 | 2023-08-16 | パナソニックホールディングス株式会社 | 膨張機およびランキンサイクル装置 |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102016107140A1 (de) * | 2016-04-18 | 2017-10-19 | Gea Mechanical Equipment Gmbh | Verfahren zur Gewinnung von zumindest einer oder mehrerer beta-Glucan-Verbindungen oder einer beta-Glucanhaltigen Feststoffsuspension aus Hefezellen |
JP6941947B2 (ja) * | 2017-02-14 | 2021-09-29 | 株式会社Adeka | 乳化組成物の乳化安定性向上剤、及びそれを含む化粧料組成物 |
KR102205829B1 (ko) | 2017-06-14 | 2021-01-21 | 기초과학연구원 | 신규한 비피도박테리움 비피덤 균주 및 균주 유래 다당체 |
KR102023317B1 (ko) * | 2017-09-18 | 2019-09-20 | 주식회사 아리바이오 | 멜라닌색소를 생산하지 않는 베타-글루칸 고생산성 오레오바시디움 풀루란스 균주 |
EP3550027A1 (en) * | 2018-04-04 | 2019-10-09 | Clariant International Ltd | Process for the fermentation of ascomycota |
JP6914534B2 (ja) * | 2018-08-30 | 2021-08-04 | 株式会社アウレオ | β−グルカン高産生菌株、β−グルカンの製造方法、及びβ−グルカン高産生菌株のスクリーニング方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3539180A1 (de) * | 1985-11-05 | 1987-05-07 | Consortium Elektrochem Ind | Aureobasidium-pullulans-stamm, herstellungsverfahren und verwendung |
JP4369258B2 (ja) * | 2004-02-05 | 2009-11-18 | 株式会社アウレオ | 免疫賦活剤 |
JP4268105B2 (ja) | 2004-09-09 | 2009-05-27 | 株式会社アウレオ | β−グルカン含有組成物、その製造方法及び該組成物を含む飲食品若しくは皮膚用保湿剤 |
JP5429843B2 (ja) | 2007-08-31 | 2014-02-26 | 独立行政法人物質・材料研究機構 | 八面体シート構造を有する光触媒材料 |
JP2010094078A (ja) * | 2008-10-16 | 2010-04-30 | Toshio Sekiguchi | 発酵飼料の製造方法 |
TWI496578B (zh) | 2009-10-08 | 2015-08-21 | Aureo Co Ltd | 流感病毒感染症的治療劑 |
JP2011254789A (ja) * | 2010-06-07 | 2011-12-22 | Oobiken:Kk | 有機醗酵飼料の製造方法 |
-
2013
- 2013-05-29 JP JP2014525745A patent/JP6053198B2/ja not_active Expired - Fee Related
- 2013-05-29 WO PCT/JP2013/064862 patent/WO2014013793A1/ja active Application Filing
- 2013-05-29 US US14/415,574 patent/US9896706B2/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7327023B2 (ja) | 2019-09-12 | 2023-08-16 | パナソニックホールディングス株式会社 | 膨張機およびランキンサイクル装置 |
Also Published As
Publication number | Publication date |
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US9896706B2 (en) | 2018-02-20 |
US20150152454A1 (en) | 2015-06-04 |
JPWO2014013793A1 (ja) | 2016-06-30 |
WO2014013793A1 (ja) | 2014-01-23 |
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