JP6043999B2 - ヒト多能性細胞培養のための簡易基本培地 - Google Patents

ヒト多能性細胞培養のための簡易基本培地 Download PDF

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JP6043999B2
JP6043999B2 JP2013523366A JP2013523366A JP6043999B2 JP 6043999 B2 JP6043999 B2 JP 6043999B2 JP 2013523366 A JP2013523366 A JP 2013523366A JP 2013523366 A JP2013523366 A JP 2013523366A JP 6043999 B2 JP6043999 B2 JP 6043999B2
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JP2013532492A5 (enExample
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ジェイムズ エイ トムソン
ジェイムズ エイ トムソン
グオカイ チェン
グオカイ チェン
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ウィスコンシン アラムニ リサーチ ファンデーション
ウィスコンシン アラムニ リサーチ ファンデーション
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JP2013523366A 2010-08-05 2011-08-05 ヒト多能性細胞培養のための簡易基本培地 Active JP6043999B2 (ja)

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US37112810P 2010-08-05 2010-08-05
US61/371,128 2010-08-05
PCT/US2011/046796 WO2012019122A2 (en) 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture

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CN (2) CN103180434A (enExample)
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Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA3114827C (en) 2007-07-31 2023-09-05 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
CA2954431C (en) 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
RU2551772C2 (ru) 2008-02-21 2015-05-27 Сентокор Орто Байотек Инк. Способы, поверхностно-модифицированные носители и композиции для иммобилизации, культивирования и открепления клеток
AU2009267137A1 (en) 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
MX2011004563A (es) 2008-10-31 2011-06-01 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
MX2011004565A (es) 2008-10-31 2011-07-28 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
EP3260534A1 (en) 2008-11-20 2017-12-27 Janssen Biotech, Inc. Pluripotent stem cell culture on micro-carriers
BRPI0921996A2 (pt) 2008-11-20 2015-08-18 Centocor Ortho Biotech Inc Métodos e composições para cultura e ligação de células em substratos planos.
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
BR112012017761A2 (pt) 2009-12-23 2015-09-15 Centocor Ortho Biotech Inc diferenciação das células-tronco embrionárias humanas
US9969981B2 (en) 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
RU2663339C1 (ru) 2010-05-12 2018-08-03 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
KR101836850B1 (ko) 2010-08-31 2018-03-09 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6133776B2 (ja) 2010-08-31 2017-05-24 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の分化
DK3260140T3 (da) 2011-12-05 2021-04-19 Factor Bioscience Inc Fremgangsmåder og produkter til transficering af celler
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US8772460B2 (en) 2011-12-16 2014-07-08 Wisconsin Alumni Research Foundation Thermostable FGF-2 mutant having enhanced stability
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
RU2018128383A (ru) 2012-03-07 2019-03-14 Янссен Байотек, Инк. Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток
RU2018108850A (ru) 2012-06-08 2019-02-26 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки
BR122019025681B1 (pt) 2012-11-01 2023-04-18 Factor Bioscience Inc Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula
SG10201707811XA (en) 2012-12-31 2017-11-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
EP4039798A1 (en) 2012-12-31 2022-08-10 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells
MX2015008577A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Cultivo de celulas madre embrionarias humanas en la interfase aire-liquido para la diferenciacion en celulas endocrinas pancreaticas.
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
SG11201505965TA (en) 2013-01-31 2015-09-29 Ajinomoto Kk Culture method for stable undifferentiated proliferation of pluripotent stem cells
WO2014208295A1 (ja) 2013-06-28 2014-12-31 株式会社カネカ 多能性幹細胞の増殖促進因子のスクリーニング法
US10137154B2 (en) * 2013-07-12 2018-11-27 Bioventures, Llc Methods and compositions for expanding long-term hematopoietic stem cell populations
KR101655383B1 (ko) * 2013-07-27 2016-09-08 고려대학교 산학협력단 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
WO2015117021A1 (en) 2014-01-31 2015-08-06 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
JP6422221B2 (ja) * 2014-03-04 2018-11-14 旭化成株式会社 多能性幹細胞からなる細胞塊製造方法
EP3124602B1 (en) 2014-03-26 2019-02-27 Kyoto University Culture medium for pluripotent stem cells
KR102162138B1 (ko) 2014-05-16 2020-10-06 얀센 바이오테크 인코포레이티드 췌장 내분비 세포에서 mafa 발현을 향상시키기 위한 소분자의 용도
EP3177302A4 (en) * 2014-08-07 2018-04-11 Duke University Compositions and methods for the reprogramming of cells into cardiomyocytes
EP3183338B1 (en) 2014-08-19 2020-03-04 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
US10626372B1 (en) 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
EP3543339A1 (en) 2015-02-13 2019-09-25 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
KR20250141836A (ko) 2015-11-04 2025-09-29 페이트 세러퓨틱스, 인코포레이티드 만능 세포의 유전자 조작
CN108473961B (zh) 2015-11-04 2022-11-29 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
CN109803977B (zh) 2016-08-17 2023-03-17 菲克特生物科学股份有限公司 核酸产品及其施用方法
KR101953573B1 (ko) * 2017-03-31 2019-03-05 한양대학교 에리카산학협력단 난황줄기세포 배양을 위한 우태아혈청 조성물 및 이의 제조방법
MX2019011897A (es) 2017-04-18 2019-11-28 Fujifilm Cellular Dynamics Inc Celulas efectoras inmunitarias especificas de antigenos.
KR102121602B1 (ko) * 2018-06-27 2020-06-10 주식회사 티아라줄기세포연구소 지방줄기세포배양용 배지 조성물 제조방법, 지방줄기세포배양용 배지 조성물 제조방법과 줄기세포 유효성분 3저 추출법을 이용한 줄기세포파쇄추출물(쉘드줄기세포) 제조방법, 이를 이용한 항관절염 치료용 조성물, 이를 이용한 염증억제 효과를 갖는 조성물 및 세포재생 효과를 갖는 조성물
EP3947642A4 (en) * 2019-04-05 2022-12-14 The University of Hong Kong CULTURE MEDIUM FOR MAMMALIAN PLURIPOTENTE STEM CELLS, COMPOSITION AND METHODS
WO2021003462A1 (en) 2019-07-03 2021-01-07 Factor Bioscience Inc. Cationic lipids and uses thereof
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
US20210087525A1 (en) * 2019-09-19 2021-03-25 Northwestern University Cost effective culture media and protocol for human induced pluripotent stem cells
CN110564673A (zh) * 2019-09-26 2019-12-13 广东工业大学 一种干细胞培养液及其应用
CN110564678B (zh) * 2019-10-12 2023-04-07 广东唯泰生物科技有限公司 脐带华通氏胶间充质干细胞成骨定向分化的方法
EP4342977A1 (en) * 2022-09-26 2024-03-27 Ares Trading S.A. Serum free medium
WO2024091801A2 (en) * 2022-10-13 2024-05-02 President And Fellows Of Harvard College Methods and compositions for inducing cell differentiation
CN119020297A (zh) * 2024-10-22 2024-11-26 江苏凯基生物技术股份有限公司 一种用于人诱导多能干细胞的化学成分限定培养基

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
ES2383813T3 (es) 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias
EP1962719A4 (en) * 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
GB0606671D0 (en) 2006-04-03 2006-05-10 Plasso Technology Ltd Cell Culture
WO2009135206A1 (en) 2008-05-02 2009-11-05 Stem Cell Products, Inc. Method for production of mast cells from stem cells
EP3450545B1 (en) 2008-10-24 2023-08-23 Wisconsin Alumni Research Foundation Pluripotent stem cells obtained by non-viral reprogramming
EP2499236B1 (en) 2009-11-12 2020-01-01 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

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CA2807418A1 (en) 2012-02-09
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KR20130099927A (ko) 2013-09-06
EP2601288B1 (en) 2016-04-06
US9279103B2 (en) 2016-03-08
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AU2011285531A1 (en) 2013-02-21
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