JP6014045B2 - 組織サンプルから細胞および細胞濃縮マトリックスを濃縮回収する方法および装置 - Google Patents

組織サンプルから細胞および細胞濃縮マトリックスを濃縮回収する方法および装置 Download PDF

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JP6014045B2
JP6014045B2 JP2013544851A JP2013544851A JP6014045B2 JP 6014045 B2 JP6014045 B2 JP 6014045B2 JP 2013544851 A JP2013544851 A JP 2013544851A JP 2013544851 A JP2013544851 A JP 2013544851A JP 6014045 B2 JP6014045 B2 JP 6014045B2
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アルト，エックハルト，ユー．
コールマン，マイケル，イー．
スタバーズ，ロン
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JP2013544851A 2010-12-16 2011-12-16 組織サンプルから細胞および細胞濃縮マトリックスを濃縮回収する方法および装置 Expired - Fee Related JP6014045B2 (ja)

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US42401210P 2010-12-16 2010-12-16
US61/424,012 2010-12-16
US201161555305P 2011-11-03 2011-11-03
US61/555,305 2011-11-03
PCT/US2011/065654 WO2012083260A2 (en) 2010-12-16 2011-12-16 Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples

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JP2014505675A JP2014505675A (ja) 2014-03-06
JP2014505675A5 JP2014505675A5 (enExample) 2015-02-12
JP6014045B2 true JP6014045B2 (ja) 2016-10-25

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CN (1) CN103402646B (enExample)
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Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11174458B2 (en) 2007-04-23 2021-11-16 Koligo Therapeutics, Inc. Cell separation apparatus and methods of use
US12239669B2 (en) 2011-07-08 2025-03-04 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US9931445B2 (en) * 2011-07-08 2018-04-03 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US10967110B2 (en) 2011-07-08 2021-04-06 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US11766459B2 (en) 2011-07-08 2023-09-26 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US10058630B2 (en) * 2012-10-22 2018-08-28 Concievalve, Llc Methods for inhibiting stenosis, obstruction, or calcification of a stented heart valve or bioprosthesis
EP3660140A1 (en) * 2014-01-31 2020-06-03 DSM IP Assets B.V. Adipose tissue processing centrifuge and methods of use
DK3430122T3 (da) * 2016-03-17 2021-08-23 Synova Life Sciences Inc Separering, adskillelse og/eller opdeling af celler ved hjælp af mekaniske stød
US10471439B2 (en) * 2016-05-17 2019-11-12 Heathrow Scientific Llc Combination centrifuge and magnetic stirrer
CN106367320B (zh) * 2016-11-21 2023-11-14 上海美致臻生物医学科技有限公司 一种手动脂肪核心集群细胞、脂肪基质血管组分采集器
JP7616798B2 (ja) * 2017-04-05 2025-01-17 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド エクスビボ培養系およびその使用方法
US11091732B2 (en) * 2017-05-21 2021-08-17 Michael A. Scarpone Methods and devices for harvesting and processing connective tissue precursor cells from autologous fat
EP3675881A4 (en) * 2017-09-01 2021-05-26 University of Pittsburgh- Of the Commonwealth System of Higher Education METHOD AND KIT FOR THE PRESERVATION OF ADAPTIVE TISSUE TRANSPLANTS
EP3772256B1 (en) * 2018-04-02 2025-05-14 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
DK180639B1 (en) 2018-06-01 2021-11-04 Apple Inc DISABILITY OF ATTENTION-ATTENTIVE VIRTUAL ASSISTANT
CN109022340A (zh) * 2018-06-28 2018-12-18 江苏云宇医疗科技有限公司 一种细胞离心方法
JP2022522323A (ja) * 2018-12-13 2022-04-18 コリゴ セラピューティクス， インコーポレイテッド 細胞分離装置および使用方法
KR102632069B1 (ko) * 2020-03-27 2024-02-02 이준석 기계적 방식에 의한 생체조직을 구성하는 조직 및 세포 분리 방법
EP4143292A2 (en) 2020-04-30 2023-03-08 Scicotec GmbH Automated integrated liposuction and tissue processing device and method
US11987787B2 (en) 2020-07-22 2024-05-21 AVITA Medical Americas, LLC Devices, methods, and kits for preparing a cell suspension
CN112067408A (zh) * 2020-10-16 2020-12-11 昆明东环科技有限公司 一种细胞块自动制备机和方法
CN112391343A (zh) * 2020-11-17 2021-02-23 华夏源(上海)生命科技有限公司 白细胞提取物中外泌体的提取方法及其在护肤品中的应用
WO2023209039A2 (en) 2022-04-27 2023-11-02 Gerlach, Jörg C. Tissue structure isolation using a micro-cutting device and uses of resulting micro-cubes of tissue
CN115870109B (zh) * 2022-12-07 2023-08-22 上海医涯医疗科技有限公司 一种水平离心转子、细胞分离装置和方法
AU2023415952A1 (en) 2022-12-27 2025-07-24 AVITA Medical Americas, LLC System for automated preparation of a regenerative epidermal suspension and related methods of use

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2097531A (en) * 1934-06-20 1937-11-02 Merco Centrifugal Separator Co Process applicable to starch manufacture
US5249711A (en) * 1992-10-01 1993-10-05 Du Pont Canada Inc. Disposable dispensing pipette
US5605529A (en) * 1996-01-17 1997-02-25 Norfolk Scientific, Inc. High efficiency centrifuge rotor
IL145002A0 (en) * 1999-03-10 2002-06-30 Univ Pittsburgh Adipose-derived stem cells and lattices
US20030082152A1 (en) * 1999-03-10 2003-05-01 Hedrick Marc H. Adipose-derived stem cells and lattices
JP3951602B2 (ja) * 2000-12-28 2007-08-01 日立工機株式会社 遠心分離機
US20030054331A1 (en) * 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
KR20040001439A (ko) * 2002-06-28 2004-01-07 (주)바이오넥스 자동화된 원심분리 시스템
AU2004248962A1 (en) * 2003-06-21 2004-12-29 Lifeforce Group Plc Leukocyte cell banks
US20050124073A1 (en) * 2003-12-09 2005-06-09 Entire Interest Fat collection and preparation system and method
US7837947B2 (en) * 2003-12-12 2010-11-23 3M Innovative Properties Company Sample mixing on a microfluidic device
US20050129752A1 (en) * 2003-12-15 2005-06-16 Tty Biopharm Limited Company Use and manufacturing process for liposomal doxorubicin pharmaceutical composition
JP2007029889A (ja) * 2005-07-28 2007-02-08 Olympus Corp 遠心分離容器および遠心分離機
KR100772969B1 (ko) * 2006-06-08 2007-11-02 양현진 원심분리기 및 원심분리방법
KR100767448B1 (ko) * 2006-06-30 2007-10-17 메디칸(주) 원심분리기 및 원심분리방법
US8071148B2 (en) * 2007-06-26 2011-12-06 Heat And Control Inc. Method for separating cooking oils from snack food products through a quasi-continuous centrifuge action
KR100848056B1 (ko) * 2007-07-30 2008-07-23 (주)프로스테믹스 줄기세포 배양액을 이용한 멜라닌 합성저해 방법
KR20090038063A (ko) * 2007-10-15 2009-04-20 이영섭 피부미용 및 성형을 위한 지방조직에서의 세포 분리 및이의 사용 방법
EP2222234B1 (en) 2007-12-04 2018-01-24 Ingeneron, Inc. Apparatus and methods for cell isolation
US8337711B2 (en) * 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
WO2009120879A1 (en) * 2008-03-26 2009-10-01 Ams Research Corporation Treatment of pelvic floor disorders with an adipose-derived cell composition
US8865199B2 (en) 2008-11-17 2014-10-21 Ingeneron, Inc. Biomatrix composition and methods of biomatrix seeding
JP5036692B2 (ja) * 2008-12-01 2012-09-26 オリンパス株式会社 細胞処理装置
JP5287364B2 (ja) * 2009-03-04 2013-09-11 日立工機株式会社 遠心分離機
KR101316573B1 (ko) * 2009-10-27 2013-10-15 도병록 재생성 세포 추출유닛 및 재생성 세포 추출시스템

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AU2011343513B2 (en) 2016-07-14
US20150307845A1 (en) 2015-10-29
BR112013015215B1 (pt) 2020-09-29
WO2012083260A2 (en) 2012-06-21
US20150314041A1 (en) 2015-11-05
WO2012083260A3 (en) 2012-12-27
US20150093809A1 (en) 2015-04-02
US20150299643A1 (en) 2015-10-22
KR101951055B1 (ko) 2019-02-21
WO2012083260A9 (en) 2012-10-26
BR112013015215A2 (pt) 2017-12-26
EP2651565A4 (en) 2016-11-09
CN103402646A (zh) 2013-11-20
EP2651565A2 (en) 2013-10-23
CN103402646B (zh) 2015-10-21
JP2014505675A (ja) 2014-03-06
EP2651565B1 (en) 2021-09-08

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