AU2011343513B2 - Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples - Google Patents
Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samplesInfo
- Publication number
- AU2011343513B2 AU2011343513B2 AU2011343513A AU2011343513A AU2011343513B2 AU 2011343513 B2 AU2011343513 B2 AU 2011343513B2 AU 2011343513 A AU2011343513 A AU 2011343513A AU 2011343513 A AU2011343513 A AU 2011343513A AU 2011343513 B2 AU2011343513 B2 AU 2011343513B2
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- tissue
- cell
- container
- cells
- acceleration
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- Ceased
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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Applications Claiming Priority (5)
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| US42401210P | 2010-12-16 | 2010-12-16 | |
| US61/424,012 | 2010-12-16 | ||
| US201161555305P | 2011-11-03 | 2011-11-03 | |
| US61/555,305 | 2011-11-03 | ||
| PCT/US2011/065654 WO2012083260A2 (en) | 2010-12-16 | 2011-12-16 | Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples |
Publications (2)
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| AU2011343513A1 AU2011343513A1 (en) | 2013-08-08 |
| AU2011343513B2 true AU2011343513B2 (en) | 2016-07-14 |
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| JP (1) | JP6014045B2 (enExample) |
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| CN (1) | CN103402646B (enExample) |
| AU (1) | AU2011343513B2 (enExample) |
| BR (1) | BR112013015215B1 (enExample) |
| WO (1) | WO2012083260A2 (enExample) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11174458B2 (en) | 2007-04-23 | 2021-11-16 | Koligo Therapeutics, Inc. | Cell separation apparatus and methods of use |
| US12239669B2 (en) | 2011-07-08 | 2025-03-04 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| US9931445B2 (en) * | 2011-07-08 | 2018-04-03 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| US10967110B2 (en) | 2011-07-08 | 2021-04-06 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| US11766459B2 (en) | 2011-07-08 | 2023-09-26 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| US10058630B2 (en) * | 2012-10-22 | 2018-08-28 | Concievalve, Llc | Methods for inhibiting stenosis, obstruction, or calcification of a stented heart valve or bioprosthesis |
| EP3660140A1 (en) * | 2014-01-31 | 2020-06-03 | DSM IP Assets B.V. | Adipose tissue processing centrifuge and methods of use |
| DK3430122T3 (da) * | 2016-03-17 | 2021-08-23 | Synova Life Sciences Inc | Separering, adskillelse og/eller opdeling af celler ved hjælp af mekaniske stød |
| US10471439B2 (en) * | 2016-05-17 | 2019-11-12 | Heathrow Scientific Llc | Combination centrifuge and magnetic stirrer |
| CN106367320B (zh) * | 2016-11-21 | 2023-11-14 | 上海美致臻生物医学科技有限公司 | 一种手动脂肪核心集群细胞、脂肪基质血管组分采集器 |
| JP7616798B2 (ja) * | 2017-04-05 | 2025-01-17 | イェダ リサーチ アンド ディベロップメント カンパニー リミテッド | エクスビボ培養系およびその使用方法 |
| US11091732B2 (en) * | 2017-05-21 | 2021-08-17 | Michael A. Scarpone | Methods and devices for harvesting and processing connective tissue precursor cells from autologous fat |
| EP3675881A4 (en) * | 2017-09-01 | 2021-05-26 | University of Pittsburgh- Of the Commonwealth System of Higher Education | METHOD AND KIT FOR THE PRESERVATION OF ADAPTIVE TISSUE TRANSPLANTS |
| EP3772256B1 (en) * | 2018-04-02 | 2025-05-14 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| DK180639B1 (en) | 2018-06-01 | 2021-11-04 | Apple Inc | DISABILITY OF ATTENTION-ATTENTIVE VIRTUAL ASSISTANT |
| CN109022340A (zh) * | 2018-06-28 | 2018-12-18 | 江苏云宇医疗科技有限公司 | 一种细胞离心方法 |
| JP2022522323A (ja) * | 2018-12-13 | 2022-04-18 | コリゴ セラピューティクス， インコーポレイテッド | 細胞分離装置および使用方法 |
| KR102632069B1 (ko) * | 2020-03-27 | 2024-02-02 | 이준석 | 기계적 방식에 의한 생체조직을 구성하는 조직 및 세포 분리 방법 |
| EP4143292A2 (en) | 2020-04-30 | 2023-03-08 | Scicotec GmbH | Automated integrated liposuction and tissue processing device and method |
| US11987787B2 (en) | 2020-07-22 | 2024-05-21 | AVITA Medical Americas, LLC | Devices, methods, and kits for preparing a cell suspension |
| CN112067408A (zh) * | 2020-10-16 | 2020-12-11 | 昆明东环科技有限公司 | 一种细胞块自动制备机和方法 |
| CN112391343A (zh) * | 2020-11-17 | 2021-02-23 | 华夏源(上海)生命科技有限公司 | 白细胞提取物中外泌体的提取方法及其在护肤品中的应用 |
| WO2023209039A2 (en) | 2022-04-27 | 2023-11-02 | Gerlach, Jörg C. | Tissue structure isolation using a micro-cutting device and uses of resulting micro-cubes of tissue |
| CN115870109B (zh) * | 2022-12-07 | 2023-08-22 | 上海医涯医疗科技有限公司 | 一种水平离心转子、细胞分离装置和方法 |
| AU2023415952A1 (en) | 2022-12-27 | 2025-07-24 | AVITA Medical Americas, LLC | System for automated preparation of a regenerative epidermal suspension and related methods of use |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2097531A (en) * | 1934-06-20 | 1937-11-02 | Merco Centrifugal Separator Co | Process applicable to starch manufacture |
| US5249711A (en) * | 1992-10-01 | 1993-10-05 | Du Pont Canada Inc. | Disposable dispensing pipette |
| US5605529A (en) * | 1996-01-17 | 1997-02-25 | Norfolk Scientific, Inc. | High efficiency centrifuge rotor |
| IL145002A0 (en) * | 1999-03-10 | 2002-06-30 | Univ Pittsburgh | Adipose-derived stem cells and lattices |
| US20030082152A1 (en) * | 1999-03-10 | 2003-05-01 | Hedrick Marc H. | Adipose-derived stem cells and lattices |
| JP3951602B2 (ja) * | 2000-12-28 | 2007-08-01 | 日立工機株式会社 | 遠心分離機 |
| US20030054331A1 (en) * | 2001-09-14 | 2003-03-20 | Stemsource, Inc. | Preservation of non embryonic cells from non hematopoietic tissues |
| US9144583B2 (en) * | 2002-03-29 | 2015-09-29 | Tissue Genesis, Inc. | Cell separation apparatus and methods of use |
| KR20040001439A (ko) * | 2002-06-28 | 2004-01-07 | (주)바이오넥스 | 자동화된 원심분리 시스템 |
| AU2004248962A1 (en) * | 2003-06-21 | 2004-12-29 | Lifeforce Group Plc | Leukocyte cell banks |
| US20050124073A1 (en) * | 2003-12-09 | 2005-06-09 | Entire Interest | Fat collection and preparation system and method |
| US7837947B2 (en) * | 2003-12-12 | 2010-11-23 | 3M Innovative Properties Company | Sample mixing on a microfluidic device |
| US20050129752A1 (en) * | 2003-12-15 | 2005-06-16 | Tty Biopharm Limited Company | Use and manufacturing process for liposomal doxorubicin pharmaceutical composition |
| JP2007029889A (ja) * | 2005-07-28 | 2007-02-08 | Olympus Corp | 遠心分離容器および遠心分離機 |
| KR100772969B1 (ko) * | 2006-06-08 | 2007-11-02 | 양현진 | 원심분리기 및 원심분리방법 |
| KR100767448B1 (ko) * | 2006-06-30 | 2007-10-17 | 메디칸(주) | 원심분리기 및 원심분리방법 |
| US8071148B2 (en) * | 2007-06-26 | 2011-12-06 | Heat And Control Inc. | Method for separating cooking oils from snack food products through a quasi-continuous centrifuge action |
| KR100848056B1 (ko) * | 2007-07-30 | 2008-07-23 | (주)프로스테믹스 | 줄기세포 배양액을 이용한 멜라닌 합성저해 방법 |
| KR20090038063A (ko) * | 2007-10-15 | 2009-04-20 | 이영섭 | 피부미용 및 성형을 위한 지방조직에서의 세포 분리 및이의 사용 방법 |
| EP2222234B1 (en) | 2007-12-04 | 2018-01-24 | Ingeneron, Inc. | Apparatus and methods for cell isolation |
| US8337711B2 (en) * | 2008-02-29 | 2012-12-25 | Biomet Biologics, Llc | System and process for separating a material |
| WO2009120879A1 (en) * | 2008-03-26 | 2009-10-01 | Ams Research Corporation | Treatment of pelvic floor disorders with an adipose-derived cell composition |
| US8865199B2 (en) | 2008-11-17 | 2014-10-21 | Ingeneron, Inc. | Biomatrix composition and methods of biomatrix seeding |
| JP5036692B2 (ja) * | 2008-12-01 | 2012-09-26 | オリンパス株式会社 | 細胞処理装置 |
| JP5287364B2 (ja) * | 2009-03-04 | 2013-09-11 | 日立工機株式会社 | 遠心分離機 |
| KR101316573B1 (ko) * | 2009-10-27 | 2013-10-15 | 도병록 | 재생성 세포 추출유닛 및 재생성 세포 추출시스템 |
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- 2011-12-16 US US13/329,143 patent/US8951513B2/en not_active Expired - Fee Related
- 2011-12-16 CN CN201180067265.8A patent/CN103402646B/zh active Active
- 2011-12-16 AU AU2011343513A patent/AU2011343513B2/en not_active Ceased
- 2011-12-16 JP JP2013544851A patent/JP6014045B2/ja not_active Expired - Fee Related
- 2011-12-16 KR KR1020137018685A patent/KR101951055B1/ko active Active
- 2011-12-16 WO PCT/US2011/065654 patent/WO2012083260A2/en not_active Ceased
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2013
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2015
- 2015-02-10 US US14/544,756 patent/US20150314041A1/en not_active Abandoned
- 2015-02-10 US US14/544,754 patent/US20150307845A1/en not_active Abandoned
- 2015-02-10 US US14/544,755 patent/US20150299643A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20140038354A (ko) | 2014-03-28 |
| US20120195863A1 (en) | 2012-08-02 |
| US8951513B2 (en) | 2015-02-10 |
| US20150307845A1 (en) | 2015-10-29 |
| BR112013015215B1 (pt) | 2020-09-29 |
| WO2012083260A2 (en) | 2012-06-21 |
| JP6014045B2 (ja) | 2016-10-25 |
| US20150314041A1 (en) | 2015-11-05 |
| WO2012083260A3 (en) | 2012-12-27 |
| US20150093809A1 (en) | 2015-04-02 |
| US20150299643A1 (en) | 2015-10-22 |
| KR101951055B1 (ko) | 2019-02-21 |
| WO2012083260A9 (en) | 2012-10-26 |
| BR112013015215A2 (pt) | 2017-12-26 |
| EP2651565A4 (en) | 2016-11-09 |
| CN103402646A (zh) | 2013-11-20 |
| EP2651565A2 (en) | 2013-10-23 |
| CN103402646B (zh) | 2015-10-21 |
| JP2014505675A (ja) | 2014-03-06 |
| EP2651565B1 (en) | 2021-09-08 |
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