CN103402646B - 用于增强从组织样品回收细胞和富含细胞的基质的方法和装置 - Google Patents

用于增强从组织样品回收细胞和富含细胞的基质的方法和装置 Download PDF

Info

Publication number
CN103402646B
CN103402646B CN201180067265.8A CN201180067265A CN103402646B CN 103402646 B CN103402646 B CN 103402646B CN 201180067265 A CN201180067265 A CN 201180067265A CN 103402646 B CN103402646 B CN 103402646B
Authority
CN
China
Prior art keywords
container
tissue
rotor
axle
acceleration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201180067265.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN103402646A (zh
Inventor
埃克哈德·U·阿尔特
迈克尔·E·科尔曼
罗恩·施图伯尔斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CN103402646A publication Critical patent/CN103402646A/zh
Application granted granted Critical
Publication of CN103402646B publication Critical patent/CN103402646B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/142—Apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B15/00—Other accessories for centrifuges
    • B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Thermal Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
CN201180067265.8A 2010-12-16 2011-12-16 用于增强从组织样品回收细胞和富含细胞的基质的方法和装置 Active CN103402646B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US42401210P 2010-12-16 2010-12-16
US61/424,012 2010-12-16
US201161555305P 2011-11-03 2011-11-03
US61/555,305 2011-11-03
PCT/US2011/065654 WO2012083260A2 (en) 2010-12-16 2011-12-16 Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples

Publications (2)

Publication Number Publication Date
CN103402646A CN103402646A (zh) 2013-11-20
CN103402646B true CN103402646B (zh) 2015-10-21

Family

ID=46245409

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180067265.8A Active CN103402646B (zh) 2010-12-16 2011-12-16 用于增强从组织样品回收细胞和富含细胞的基质的方法和装置

Country Status (8)

Country Link
US (5) US8951513B2 (enExample)
EP (1) EP2651565B1 (enExample)
JP (1) JP6014045B2 (enExample)
KR (1) KR101951055B1 (enExample)
CN (1) CN103402646B (enExample)
AU (1) AU2011343513B2 (enExample)
BR (1) BR112013015215B1 (enExample)
WO (1) WO2012083260A2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11174458B2 (en) 2007-04-23 2021-11-16 Koligo Therapeutics, Inc. Cell separation apparatus and methods of use
US11766459B2 (en) 2011-07-08 2023-09-26 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US9931445B2 (en) * 2011-07-08 2018-04-03 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US12239669B2 (en) 2011-07-08 2025-03-04 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US10967110B2 (en) 2011-07-08 2021-04-06 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
US10058630B2 (en) * 2012-10-22 2018-08-28 Concievalve, Llc Methods for inhibiting stenosis, obstruction, or calcification of a stented heart valve or bioprosthesis
CN106413904A (zh) * 2014-01-31 2017-02-15 帝斯曼知识产权资产管理有限公司 脂肪组织离心装置和使用方法
BR112018068820B1 (pt) * 2016-03-17 2022-11-16 Synova Life Sciences, Inc Método para isolar células-tronco do tecido adiposo e dispositivo para realizar o dito método
US10471439B2 (en) * 2016-05-17 2019-11-12 Heathrow Scientific Llc Combination centrifuge and magnetic stirrer
CN106367320B (zh) * 2016-11-21 2023-11-14 上海美致臻生物医学科技有限公司 一种手动脂肪核心集群细胞、脂肪基质血管组分采集器
FI3607050T3 (fi) * 2017-04-05 2023-11-20 Yeda Res & Dev Ex vivo viljelyjärjestelmä ja menetelmiä sen käyttämiseksi
US11091732B2 (en) * 2017-05-21 2021-08-17 Michael A. Scarpone Methods and devices for harvesting and processing connective tissue precursor cells from autologous fat
CA3074457A1 (en) * 2017-09-01 2019-03-07 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Method and kit for preservation of adipose tissue grafts
EP3772256B1 (en) * 2018-04-02 2025-05-14 Jointechlabs, Inc. System and methods for preparation of adipose-derived stem cells
DK180639B1 (en) 2018-06-01 2021-11-04 Apple Inc DISABILITY OF ATTENTION-ATTENTIVE VIRTUAL ASSISTANT
CN109022340A (zh) * 2018-06-28 2018-12-18 江苏云宇医疗科技有限公司 一种细胞离心方法
CA3123386A1 (en) * 2018-12-13 2020-06-18 Tissue Genesis, Llc Cell separation apparatus and methods of use
KR102632069B1 (ko) * 2020-03-27 2024-02-02 이준석 기계적 방식에 의한 생체조직을 구성하는 조직 및 세포 분리 방법
WO2021220057A2 (en) 2020-04-30 2021-11-04 Scicotec Gmbh Automated integrated liposuction and tissue processing device and method
US11987787B2 (en) 2020-07-22 2024-05-21 AVITA Medical Americas, LLC Devices, methods, and kits for preparing a cell suspension
CN112067408A (zh) * 2020-10-16 2020-12-11 昆明东环科技有限公司 一种细胞块自动制备机和方法
CN112391343A (zh) * 2020-11-17 2021-02-23 华夏源(上海)生命科技有限公司 白细胞提取物中外泌体的提取方法及其在护肤品中的应用
US20250288721A1 (en) 2022-04-27 2025-09-18 Jörg C. GERLACH Tissue structure isolation using a micro-cutting device and uses of resulting micro-cubes of tissue
CN115870109B (zh) * 2022-12-07 2023-08-22 上海医涯医疗科技有限公司 一种水平离心转子、细胞分离装置和方法
EP4638696A1 (en) 2022-12-27 2025-10-29 AVITA Medical Americas, LLC System for automated preparation of a regenerative epidermal suspension and related methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1352696A (zh) * 1999-03-10 2002-06-05 匹兹堡大学联邦制高等教育 脂肪来源的干细胞和网格
WO2009120879A1 (en) * 2008-03-26 2009-10-01 Ams Research Corporation Treatment of pelvic floor disorders with an adipose-derived cell composition

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2097531A (en) * 1934-06-20 1937-11-02 Merco Centrifugal Separator Co Process applicable to starch manufacture
US5249711A (en) * 1992-10-01 1993-10-05 Du Pont Canada Inc. Disposable dispensing pipette
US5605529A (en) * 1996-01-17 1997-02-25 Norfolk Scientific, Inc. High efficiency centrifuge rotor
US20030082152A1 (en) * 1999-03-10 2003-05-01 Hedrick Marc H. Adipose-derived stem cells and lattices
JP3951602B2 (ja) * 2000-12-28 2007-08-01 日立工機株式会社 遠心分離機
US20030054331A1 (en) * 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
KR20040001439A (ko) * 2002-06-28 2004-01-07 (주)바이오넥스 자동화된 원심분리 시스템
AU2004248962A1 (en) * 2003-06-21 2004-12-29 Lifeforce Group Plc Leukocyte cell banks
US20050124073A1 (en) * 2003-12-09 2005-06-09 Entire Interest Fat collection and preparation system and method
US7837947B2 (en) * 2003-12-12 2010-11-23 3M Innovative Properties Company Sample mixing on a microfluidic device
US20050129752A1 (en) * 2003-12-15 2005-06-16 Tty Biopharm Limited Company Use and manufacturing process for liposomal doxorubicin pharmaceutical composition
JP2007029889A (ja) * 2005-07-28 2007-02-08 Olympus Corp 遠心分離容器および遠心分離機
KR100772969B1 (ko) * 2006-06-08 2007-11-02 양현진 원심분리기 및 원심분리방법
KR100767448B1 (ko) * 2006-06-30 2007-10-17 메디칸(주) 원심분리기 및 원심분리방법
US8071148B2 (en) * 2007-06-26 2011-12-06 Heat And Control Inc. Method for separating cooking oils from snack food products through a quasi-continuous centrifuge action
KR100848056B1 (ko) * 2007-07-30 2008-07-23 (주)프로스테믹스 줄기세포 배양액을 이용한 멜라닌 합성저해 방법
KR20090038063A (ko) * 2007-10-15 2009-04-20 이영섭 피부미용 및 성형을 위한 지방조직에서의 세포 분리 및이의 사용 방법
US8309342B2 (en) 2007-12-04 2012-11-13 Ingeneron, Inc. Apparatus and methods for cell isolation
EP2254991B1 (en) * 2008-02-29 2018-08-22 Biomet Manufacturing, LLC A system and process for separating a material
US8865199B2 (en) 2008-11-17 2014-10-21 Ingeneron, Inc. Biomatrix composition and methods of biomatrix seeding
JP5036692B2 (ja) * 2008-12-01 2012-09-26 オリンパス株式会社 細胞処理装置
JP5287364B2 (ja) * 2009-03-04 2013-09-11 日立工機株式会社 遠心分離機
KR101316573B1 (ko) * 2009-10-27 2013-10-15 도병록 재생성 세포 추출유닛 및 재생성 세포 추출시스템

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1352696A (zh) * 1999-03-10 2002-06-05 匹兹堡大学联邦制高等教育 脂肪来源的干细胞和网格
WO2009120879A1 (en) * 2008-03-26 2009-10-01 Ams Research Corporation Treatment of pelvic floor disorders with an adipose-derived cell composition

Also Published As

Publication number Publication date
US8951513B2 (en) 2015-02-10
KR101951055B1 (ko) 2019-02-21
AU2011343513B2 (en) 2016-07-14
JP2014505675A (ja) 2014-03-06
US20150307845A1 (en) 2015-10-29
JP6014045B2 (ja) 2016-10-25
US20120195863A1 (en) 2012-08-02
KR20140038354A (ko) 2014-03-28
WO2012083260A3 (en) 2012-12-27
BR112013015215B1 (pt) 2020-09-29
BR112013015215A2 (pt) 2017-12-26
WO2012083260A9 (en) 2012-10-26
EP2651565A2 (en) 2013-10-23
US20150299643A1 (en) 2015-10-22
US20150314041A1 (en) 2015-11-05
CN103402646A (zh) 2013-11-20
EP2651565B1 (en) 2021-09-08
US20150093809A1 (en) 2015-04-02
EP2651565A4 (en) 2016-11-09
WO2012083260A2 (en) 2012-06-21

Similar Documents

Publication Publication Date Title
CN103402646B (zh) 用于增强从组织样品回收细胞和富含细胞的基质的方法和装置
AU2011343513A1 (en) Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples
CN1842589B (zh) 用于从组织分离并且浓缩再生细胞的系统和方法
CN102458302B (zh) 用于优化组织和细胞富集的移植物的系统、方法和组合物
US7514075B2 (en) Systems and methods for separating and concentrating adipose derived stem cells from tissue
US20110086426A1 (en) Methods and apparatus for collecting and separating regenerative cells from adipose tissue
CN101443023A (zh) 使用脂肪组织来源的细胞治疗心血管病症的方法
US20160272943A1 (en) Apparatus and method for closed system recovery of cells from tissue samples
KR101044371B1 (ko) 줄기세포 분리를 위한 혼합장치
US20130115697A1 (en) Methods and apparatus for enhanced recovery of cells from tissue samples
EP4143292A2 (en) Automated integrated liposuction and tissue processing device and method
HK1096424B (en) Systems and methods for separating and concentrating regenerative cells from tissue
HK1169301B (en) Systems, methods and compositions for optimizing tissue and cell enriched grafts
HK1180602A (en) Method for curing cardiovascular diseases by using cells from adipose tissue
HK1132918A (en) Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant