JP5992836B2 - ペプチドを精製するための分取ｒｐ−ｈｐｌｃ法 - Google Patents
ペプチドを精製するための分取ｒｐ−ｈｐｌｃ法 Download PDFInfo
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- JP5992836B2 JP5992836B2 JP2012555389A JP2012555389A JP5992836B2 JP 5992836 B2 JP5992836 B2 JP 5992836B2 JP 2012555389 A JP2012555389 A JP 2012555389A JP 2012555389 A JP2012555389 A JP 2012555389A JP 5992836 B2 JP5992836 B2 JP 5992836B2
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- peptide
- polypeptide
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- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 150000004715 keto acids Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229960002701 liraglutide Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 1
- 125000001419 myristoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000004810 partition chromatography Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229950000964 pepstatin Drugs 0.000 description 1
- 108010091212 pepstatin Proteins 0.000 description 1
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 150000003009 phosphonic acids Chemical class 0.000 description 1
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 229920000307 polymer substrate Chemical class 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004237 preparative chromatography Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000006340 racemization Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000002821 scintillation proximity assay Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003460 sulfonic acids Chemical class 0.000 description 1
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- ILWRPSCZWQJDMK-UHFFFAOYSA-N triethylazanium;chloride Chemical compound Cl.CCN(CC)CC ILWRPSCZWQJDMK-UHFFFAOYSA-N 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10155023.4 | 2010-03-01 | ||
| EP10155023 | 2010-03-01 | ||
| US30964210P | 2010-03-02 | 2010-03-02 | |
| US61/309,642 | 2010-03-02 | ||
| PCT/EP2011/052964 WO2011107447A1 (en) | 2010-03-01 | 2011-03-01 | Preparative rp-hplc method for purifying peptides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013521485A JP2013521485A (ja) | 2013-06-10 |
| JP2013521485A5 JP2013521485A5 (enExample) | 2014-04-17 |
| JP5992836B2 true JP5992836B2 (ja) | 2016-09-14 |
Family
ID=42727392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012555389A Expired - Fee Related JP5992836B2 (ja) | 2010-03-01 | 2011-03-01 | ペプチドを精製するための分取ｒｐ−ｈｐｌｃ法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9422330B2 (enExample) |
| EP (1) | EP2542565A1 (enExample) |
| JP (1) | JP5992836B2 (enExample) |
| CN (1) | CN102770440A (enExample) |
| WO (1) | WO2011107447A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012313045A1 (en) * | 2011-09-22 | 2014-04-10 | Hochschule Ostwestfalen-Lippe | Method for producing peptide fractions and use thereof |
| US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| US9724622B2 (en) | 2013-01-29 | 2017-08-08 | Neuland Health Sciences Private Limited | Purification of organic compounds using surrogate stationary phases on reversed phase columns |
| EP2976331B1 (en) | 2013-03-21 | 2017-03-01 | Sanofi-Aventis Deutschland GmbH | Synthesis of hydantoin containing peptide products |
| MX365465B (es) | 2013-03-21 | 2019-06-04 | Sanofi Aventis Deutschland | Sintesis de productos peptidicos que contienen imida ciclica. |
| CA2924821A1 (en) * | 2013-09-20 | 2015-03-26 | Davuluri, Ramamohan Rao | Purification of organic compounds by surfactant mediated preparative hplc |
| CN105223296B (zh) * | 2015-10-16 | 2017-12-05 | 江苏开元药业有限公司 | 一类多肽的纯化方法 |
| EP3433268B1 (en) | 2016-03-23 | 2021-07-28 | Bachem Holding AG | Purification of glucagon-like peptide 1 analogs |
| US12269844B2 (en) | 2019-03-25 | 2025-04-08 | Scinopharm Taiwan, Ltd. | Process for purifying semaglutide and liraglutide |
| TWI738260B (zh) | 2019-03-25 | 2021-09-01 | 台灣神隆股份有限公司 | 純化利拉魯肽之方法 |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| KR102691149B1 (ko) | 2021-01-20 | 2024-08-05 | 바이킹 테라퓨틱스 인코포레이티드 | 대사 및 간 질환 치료를 위한 조성물 및 방법 |
| CN115703825B (zh) * | 2021-08-17 | 2026-01-02 | 派格生物医药(杭州)股份有限公司 | 艾塞那肽变体及其聚乙二醇缀合物的制备方法 |
| US12421282B2 (en) | 2021-09-15 | 2025-09-23 | Viking Therapeutics, Inc. | Compositions and methods for the treatment of metabolic and liver disorders |
| CN114656550A (zh) * | 2021-12-28 | 2022-06-24 | 深圳翰宇药业股份有限公司 | 一种利拉鲁肽/索马鲁肽的纯化方法及应用 |
| WO2024147150A1 (en) * | 2023-01-02 | 2024-07-11 | Zelle Biotechnology Pvt. Ltd. | Chromatographic detection, estimation and separation of amino acids in hydrolysates of monoclonal antibodies |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4028120C2 (de) * | 1990-09-05 | 1996-09-19 | Hoechst Ag | Verfahren zur Reinigung von Insulin und/oder Insulinderivaten |
| US5424286A (en) | 1993-05-24 | 1995-06-13 | Eng; John | Exendin-3 and exendin-4 polypeptides, and pharmaceutical compositions comprising same |
| US6767887B1 (en) | 1996-06-05 | 2004-07-27 | Roche Diagnostics Gmbh | Exendin analogues, processes for their preparation and medicaments containing them |
| CA2264243C (en) * | 1996-08-30 | 2004-10-05 | Novo Nordisk A/S | Glp-1 derivatives |
| EP1076066A1 (en) | 1999-07-12 | 2001-02-14 | Zealand Pharmaceuticals A/S | Peptides for lowering blood glucose levels |
| JP2007526235A (ja) | 2003-08-21 | 2007-09-13 | ノボ ノルディスク アクティーゼルスカブ | グルカゴン様ペプチドの精製 |
| CN100535007C (zh) * | 2003-08-21 | 2009-09-02 | 诺沃挪第克公司 | 胰高血糖素样肽的纯化 |
| WO2007071768A1 (en) | 2005-12-23 | 2007-06-28 | Novo Nordisk A/S | Purification of proteins using preparative reverse phase chromatography (rpc) |
| WO2007071767A1 (en) | 2005-12-23 | 2007-06-28 | Novo Nordisk Health Care Ag | Purification of vitamin k-dependent polypeptides using preparative reverse phase chromatography (rpc) |
-
2011
- 2011-03-01 EP EP11705589A patent/EP2542565A1/en not_active Withdrawn
- 2011-03-01 US US13/580,204 patent/US9422330B2/en not_active Expired - Fee Related
- 2011-03-01 WO PCT/EP2011/052964 patent/WO2011107447A1/en not_active Ceased
- 2011-03-01 JP JP2012555389A patent/JP5992836B2/ja not_active Expired - Fee Related
- 2011-03-01 CN CN2011800118239A patent/CN102770440A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011107447A1 (en) | 2011-09-09 |
| EP2542565A1 (en) | 2013-01-09 |
| CN102770440A (zh) | 2012-11-07 |
| US9422330B2 (en) | 2016-08-23 |
| JP2013521485A (ja) | 2013-06-10 |
| US20120322976A1 (en) | 2012-12-20 |
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