JP5973407B2 - マイクロフルイディックシステム - Google Patents

マイクロフルイディックシステム Download PDF

Info

Publication number
JP5973407B2
JP5973407B2 JP2013203694A JP2013203694A JP5973407B2 JP 5973407 B2 JP5973407 B2 JP 5973407B2 JP 2013203694 A JP2013203694 A JP 2013203694A JP 2013203694 A JP2013203694 A JP 2013203694A JP 5973407 B2 JP5973407 B2 JP 5973407B2
Authority
JP
Japan
Prior art keywords
plug
plugs
fluid
component
array
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2013203694A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014036956A5 (cg-RX-API-DMAC10.html
JP2014036956A (ja
Inventor
エフ． イスマギロフ ラステム
エフ． イスマギロフ ラステム
ボー チェン
ボー チェン
ジェイ ガーツ コーリー
ジェイ ガーツ コーリー
Original Assignee
ユニバーシティ オブ シカゴ
ユニバーシティ オブ シカゴ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/082,187 external-priority patent/US7655470B2/en
Application filed by ユニバーシティ オブ シカゴ, ユニバーシティ オブ シカゴ filed Critical ユニバーシティ オブ シカゴ
Publication of JP2014036956A publication Critical patent/JP2014036956A/ja
Publication of JP2014036956A5 publication Critical patent/JP2014036956A5/ja
Application granted granted Critical
Publication of JP5973407B2 publication Critical patent/JP5973407B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D7/00—Control of flow
    • G05D7/06—Control of flow characterised by the use of electric means
    • G05D7/0617—Control of flow characterised by the use of electric means specially adapted for fluid materials
    • G05D7/0629—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means
    • G05D7/0694—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means by action on throttling means or flow sources of very small size, e.g. microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/30—Injector mixers
    • B01F25/31—Injector mixers in conduits or tubes through which the main component flows
    • B01F25/314—Injector mixers in conduits or tubes through which the main component flows wherein additional components are introduced at the circumference of the conduit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00513—Essentially linear supports
    • B01J2219/0052—Essentially linear supports in the shape of elongated tubes
    • B01J2219/00522—Essentially linear supports in the shape of elongated tubes in a multiple parallel arrangement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00599—Solution-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Automation & Control Theory (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micromachines (AREA)
  • Peptides Or Proteins (AREA)
JP2013203694A 2005-03-16 2013-09-30 マイクロフルイディックシステム Expired - Fee Related JP5973407B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/082,187 2005-03-16
US11/082,187 US7655470B2 (en) 2004-10-29 2005-03-16 Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US11/174,298 2005-07-01
US11/174,298 US9477233B2 (en) 2004-07-02 2005-07-01 Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2011231160A Division JP5671434B2 (ja) 2005-03-16 2011-10-20 マイクロフルイディックシステム

Publications (3)

Publication Number Publication Date
JP2014036956A JP2014036956A (ja) 2014-02-27
JP2014036956A5 JP2014036956A5 (cg-RX-API-DMAC10.html) 2014-10-09
JP5973407B2 true JP5973407B2 (ja) 2016-08-23

Family

ID=37024341

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013203694A Expired - Fee Related JP5973407B2 (ja) 2005-03-16 2013-09-30 マイクロフルイディックシステム

Country Status (4)

Country Link
US (5) US9477233B2 (cg-RX-API-DMAC10.html)
EP (2) EP1866092B1 (cg-RX-API-DMAC10.html)
JP (1) JP5973407B2 (cg-RX-API-DMAC10.html)
WO (1) WO2006101851A2 (cg-RX-API-DMAC10.html)

Families Citing this family (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20060280029A1 (en) * 2005-06-13 2006-12-14 President And Fellows Of Harvard College Microfluidic mixer
US20070068573A1 (en) * 2005-08-22 2007-03-29 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
CN101395267B (zh) * 2006-02-03 2013-03-27 哈佛学院董事会 在聚合膜上的工程细胞生产及其在生物工程方面的应用
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US20100196432A1 (en) * 2006-10-10 2010-08-05 Feinberg Adam W Biopolymer structures
US7918831B2 (en) * 2006-10-12 2011-04-05 Colorado Catheter Company, Inc. Catheter assembly having protective sheath
FR2907228B1 (fr) * 2006-10-13 2009-07-24 Rhodia Recherches & Tech Dispositif d'ecoulement fluidique,ensemble de determination d'au moins une caracteristique d'un systeme physico-chimique comprenant un tel dispositif,procede de determination et procede de criblage correspondants
FR2907227B1 (fr) * 2006-10-13 2009-04-10 Rhodia Recherches & Tech Procede et installation de determination d'au moins un parametre d'une transformation physique et/ou chimique,et procede de criblage correspondant
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
JP5062399B2 (ja) * 2007-02-19 2012-10-31 セイコーインスツル株式会社 マイクロ流路およびマイクロリアクタ
US9152150B1 (en) * 2007-02-22 2015-10-06 Applied Biosystems, Llc Compositions, systems, and methods for immiscible fluid discrete volume manipulation
EP2121186B1 (fr) * 2007-03-05 2019-10-30 Rhodia Opérations Procede de suivi de la cristallisation d'une substance
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20120309648A1 (en) * 2007-07-06 2012-12-06 The Regents Of The University Of Calfornia Integrated microfluidics for highly parallel screening of chemical reactions
US9090885B2 (en) 2007-07-26 2015-07-28 The University Of Chicago Co-incubating confined microbial communities
US9795697B2 (en) 2007-09-19 2017-10-24 Opko Diagnostics, Llc Liquid containment for integrated assays
WO2009085067A2 (en) * 2007-09-26 2009-07-09 President And Fellows Of Harvard College Boundary conditions for the arrangement of cells and tissues
FR2928209B1 (fr) * 2008-03-03 2011-04-22 Rhodia Operations Procede et installation de determination d'au moins un parametre d'une transformation physique et/ou chimique
EP2285491A1 (en) 2008-04-25 2011-02-23 Claros Diagnostics, Inc. Flow control in microfluidic systems
WO2010011407A2 (en) * 2008-05-23 2010-01-28 President And Fellows Of Harvard College Methods of generating patterned soft substrates and uses thereof
WO2009149257A1 (en) * 2008-06-04 2009-12-10 The University Of Chicago The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
KR101767125B1 (ko) 2008-06-06 2017-08-10 바이오나노 제노믹스, 인크. 통합 나노유체 분석 장치, 제작 방법 및 분석 기술
EP2352591A4 (en) * 2008-06-13 2013-01-23 Emerald Biostructures Inc NANOVOLUMIC MICROKAPILLAR CRYSTALLIZATION SYSTEM
US8475747B1 (en) * 2008-06-13 2013-07-02 U.S. Department Of Energy Processing fissile material mixtures containing zirconium and/or carbon
GB0810990D0 (en) * 2008-06-16 2008-07-23 Q Chip Ltd Device and method of making solid beads
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8053239B2 (en) * 2008-10-08 2011-11-08 The Governing Council Of The University Of Toronto Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures
US9713802B2 (en) * 2009-01-13 2017-07-25 Kobe Steel, Ltd. Method and apparatus for manufacturing liquid microspheres
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
AU2010229490B2 (en) 2009-03-24 2015-02-12 University Of Chicago Slip chip device and methods
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9012172B2 (en) 2009-05-01 2015-04-21 President And Fellows Of Harvard College Devices comprising muscle thin films and uses thereof in high throughput assays for determining contractile function
US20100298602A1 (en) * 2009-05-19 2010-11-25 Massachusetts Institute Of Technology Systems and methods for microfluidic crystallization
US20100294986A1 (en) * 2009-05-19 2010-11-25 Massachusetts Institute Of Technology Supercritical fluid facilitated particle formation in microfluidic systems
US9752964B1 (en) * 2009-06-22 2017-09-05 Stc.Unm Flow cytometry apparatus pulling sample stream through observation chamber
US8337783B2 (en) * 2009-06-23 2012-12-25 The United States of America as represented by the Secretary of Commerce, the National Institute of Standards and Technology Magnetic connectors for microfluidic applications
CA2766795C (en) * 2009-06-26 2017-10-03 President And Fellows Of Harvard College Fluid injection
US10751464B2 (en) 2009-08-25 2020-08-25 Nanoshell Company, Llc Therapeutic retrieval of targets in biological fluids
US10099227B2 (en) 2009-08-25 2018-10-16 Nanoshell Company, Llc Method and apparatus for continuous removal of sub-micron sized particles in a closed loop liquid flow system
US20140008301A1 (en) * 2012-07-05 2014-01-09 Nanoshell Company, Llc Therapeutic retrieval of targets in biological fluids
CN102625710A (zh) 2009-08-25 2012-08-01 艾格尼丝·奥斯塔芬 携氧的抗湍流的高密度亚微米颗粒的合成
US11285494B2 (en) 2009-08-25 2022-03-29 Nanoshell Company, Llc Method and apparatus for continuous removal of sub-micron sized particles in a closed loop liquid flow system
EP2473263B1 (en) 2009-09-02 2022-11-02 President and Fellows of Harvard College Multiple emulsions created using jetting and other techniques
FR2950544B1 (fr) * 2009-09-29 2011-12-09 Ecole Polytech Circuit microfluidique
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US20110165037A1 (en) * 2010-01-07 2011-07-07 Ismagilov Rustem F Interfaces that eliminate non-specific adsorption, and introduce specific interactions
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
JP6042803B2 (ja) 2010-04-26 2016-12-14 レオセンス，インコーポレイテッド 携帯型粘度計
WO2012006320A1 (en) 2010-07-06 2012-01-12 President And Fellows Of Harvard College Photosensitive cardiac rhythm modulation systems
US9677979B2 (en) 2010-07-16 2017-06-13 Vanderbilt University Low resource processor using surface tension valves for extracting, concentrating and detecting molecular species
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
IT1404074B1 (it) * 2010-12-17 2013-11-08 Alifax Holding S P A Dispositivo per realizzare test clinici su liquidi biologici e relativo procedimento
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
EP2729238A2 (en) 2011-07-06 2014-05-14 President and Fellows of Harvard College Multiple emulsions and techniques for the formation of multiple emulsions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CN103946712A (zh) * 2011-09-30 2014-07-23 不列颠哥伦比亚大学 用于流动控制润湿的方法和设备
JP5800195B2 (ja) * 2011-11-25 2015-10-28 国立研究開発法人産業技術総合研究所 結晶成長用容器、液滴調製器具および結晶取得方法
WO2013086512A2 (en) 2011-12-09 2013-06-13 President And Fellows Of Harvard College Muscle chips and methods of use thereof
WO2013159116A1 (en) 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
US9822356B2 (en) 2012-04-20 2017-11-21 California Institute Of Technology Fluidic devices and systems for sample preparation or autonomous analysis
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
US9669141B2 (en) 2012-07-20 2017-06-06 President And Fellows Of Harvard College Tissue-engineered pumps and valves and uses thereof
JP6164580B2 (ja) * 2013-03-13 2017-07-19 国立大学法人 筑波大学 マイクロ流路装置及びこれに関する方法
US9869675B2 (en) 2013-03-13 2018-01-16 Vanderbilt University Low resource processor using surface tension valves for extracting, concentrating, and detecting whole cells
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US10591458B2 (en) 2014-02-18 2020-03-17 President And Fellows Of Harvard College Anisotropic muscular tissue devices with integrated electrical force readouts
JP6090208B2 (ja) 2014-02-27 2017-03-08 マツダ株式会社 エンジンの排気装置
EP3155399B1 (en) 2014-04-11 2020-12-02 Rheosense Inc. Viscometer and methods for using the same
CA2956028C (en) * 2014-08-06 2022-06-14 Etablissements J. Soufflet A method for fusing or contacting reactor and reagent droplets in a microfluidic or millifluidic device
WO2016069142A2 (en) 2014-09-24 2016-05-06 President And Fellows Of Harvard College Contractile function measuring devices, systems, and methods of use thereof
US10018040B2 (en) * 2014-10-24 2018-07-10 Schlumberger Technology Corporation System and methodology for chemical constituent sensing and analysis
US11123740B2 (en) 2015-06-29 2021-09-21 Arizona Board Of Regents On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10357770B2 (en) * 2015-10-09 2019-07-23 International Business Machines Corporation Microfluidic probe for modulating insertion of liquid spacers
US11384328B2 (en) 2015-11-18 2022-07-12 President And Fellows Of Harvard College Cartridge-based system for long term culture of cell clusters
US10751685B2 (en) 2016-05-02 2020-08-25 Purdue Research Foundation Systems and methods for producing a chemical product
EP3269444A1 (en) * 2016-07-14 2018-01-17 Base4 Innovation Ltd Method of identifying droplets in a stack and an associated sequencer
GB201615320D0 (en) * 2016-09-09 2016-10-26 Invitron Ltd Point of care platform test
CN110740813B (zh) 2016-11-28 2022-06-03 亚利桑那州立大学董事会 涉及连续流动液滴反应的系统和方法
WO2018208448A1 (en) 2017-05-11 2018-11-15 Emd Millipore Corporation Method of maintaining narrow residence time distributions in continuous flow systems
EP3621715A4 (en) 2017-05-11 2021-01-27 EMD Millipore Corporation MECHANICAL PROCEDURE FOR MAINTAINING TIGHT DISTRIBUTION OF DWELL TIME IN CONTINUOUS FLOW SYSTEMS
CN110799679A (zh) * 2017-06-20 2020-02-14 10X基因组学有限公司 用于改善液滴稳定的方法和系统
CN107328949B (zh) * 2017-06-27 2019-05-03 中国科学院长春光学精密机械与物理研究所 一种实现多份液体自发顺序流动的方法及装置
US11499183B2 (en) 2017-06-28 2022-11-15 Bio-Rad Laboratories, Inc. System and method for droplet detection
US11166920B2 (en) * 2017-10-05 2021-11-09 Auburn University Microfluidics device for fabrication of large, uniform, injectable hydrogel microparticles for cell encapsulation
US11629318B2 (en) 2017-10-20 2023-04-18 President And Fellows Of Harvard College Methods for producing mature adipocytes and methods of use thereof
US11045805B2 (en) * 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
CN112236218B (zh) 2018-04-02 2022-04-26 滴管公司 用于连续流乳液处理的系统和方法
CN109012774B (zh) * 2018-08-10 2022-03-15 深圳先进技术研究院 液滴生成装置、液滴微流控芯片及应用
CN109622080B (zh) * 2019-01-08 2021-04-06 新羿制造科技（北京）有限公司 用于多指标分析的微液滴生成芯片
CN111434377B (zh) * 2019-01-11 2022-07-15 中国石油化工股份有限公司 一种盘管微反应器和一种微反应器系统
EP4041444B1 (en) * 2019-10-02 2024-08-28 Piotr Jeuté A method and system for controlling drop collisions in a drop on demand printing apparatus
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
WO2021110896A1 (en) * 2019-12-04 2021-06-10 Astraveus Microfluidic device and method for processing particles
CN111135883B (zh) * 2019-12-31 2024-01-02 中山大学 一种用于筛选晶体生成条件的超高通量平台及筛选方法
EP4090987A4 (en) * 2020-01-17 2024-01-17 Zepto Life Technology, LLC SYSTEMS AND METHODS FOR ANALYTE DETECTION IN MRG DETECTION OF BIOMARKERS
USD989342S1 (en) * 2020-02-04 2023-06-13 Ut-Battelle, Llc Microfluidic polymer chip interface bracket
USD993443S1 (en) * 2020-02-04 2023-07-25 Ut-Battelle, Llc Microfluidic glass chip interface bracket
US10953404B1 (en) 2020-04-24 2021-03-23 Pattern Bioscience, Inc. Apparatuses for contactless loading and imaging of microfluidic chips and related methods
GB202006525D0 (en) 2020-05-04 2020-06-17 Sphere Fluidics Ltd Surfactant
CN112461768B (zh) * 2020-11-20 2021-11-05 武汉大学 海水硝酸盐检测装置
US12078582B2 (en) 2021-05-10 2024-09-03 Rheosense, Inc. Viscometer with reduced dead-volume and high dynamic range
ES3052960T3 (en) * 2022-04-01 2026-01-16 Hoffmann La Roche Method for sensor calibration and in-vitro diagnostic analyzer
US20240085406A1 (en) * 2022-09-13 2024-03-14 Biological Mimetics, Inc. Multi-chamber device for detecting pathogens/molecules and methods of using same
WO2024102720A2 (en) * 2022-11-07 2024-05-16 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions
KR102959505B1 (ko) 2022-11-15 2026-05-07 고려대학교 산학협력단 초점도 유체의 액적화 장치
WO2024106872A1 (ko) * 2022-11-15 2024-05-23 고려대학교 산학협력단 초점도 유체의 액적화 장치
US20240198336A1 (en) * 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics
WO2025052041A1 (en) * 2023-09-06 2025-03-13 Helsingin Yliopisto Microfluidic devices and methods for producing droplets for droplet-based assays

Family Cites Families (205)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2692800A (en) 1951-10-08 1954-10-26 Gen Electric Nozzle flow control
US3479141A (en) 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
CH503985A (de) * 1968-09-19 1971-02-28 Ceskoslovenska Akademie Ved Verfahren und Einrichtung zur Trennung eines Hauptmediums von einem Strom, der durch einzelne Abschnitte des Hauptmediums und eines Trennmediums gebildet wird
BE788754A (cg-RX-API-DMAC10.html) * 1971-10-13 1973-03-13 Technicon Instr
US3816331A (en) 1972-07-05 1974-06-11 Ncr Continuous encapsulation and device therefor
CH564966A5 (cg-RX-API-DMAC10.html) 1974-02-25 1975-08-15 Sauter Fr Ag Fabrik Elektrisch
US4059552A (en) 1974-06-21 1977-11-22 The Dow Chemical Company Cross-linked water-swellable polymer particles
US3960187A (en) * 1974-07-23 1976-06-01 Usm Corporation Method and device for metering and dispersing fluid materials
US3982541A (en) 1974-07-29 1976-09-28 Esperance Jr Francis A L Eye surgical instrument
FI52025C (fi) * 1976-04-08 1977-06-10 Osmo Antero Suovaniemi Menetelmä ja laitteisto nesteannosteluun, nesteen siirtoon ja laimennu ssarjoihin.
US4279345A (en) 1979-08-03 1981-07-21 Allred John C High speed particle sorter using a field emission electrode
US4346608A (en) * 1980-07-31 1982-08-31 The United States Of America As Represented By The Secretary Of The Army Float device for density gradient fractionation
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
JPS6057907B2 (ja) 1981-06-18 1985-12-17 工業技術院長 液体の混合噴霧化方法
DE3230289A1 (de) 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen Herstellung von pharmazeutischen oder kosmetischen dispersionen
US4853336A (en) * 1982-11-15 1989-08-01 Technicon Instruments Corporation Single channel continuous flow system
US4618476A (en) 1984-02-10 1986-10-21 Eastman Kodak Company Capillary transport device having speed and meniscus control means
US4865444A (en) 1984-04-05 1989-09-12 Mobil Oil Corporation Apparatus and method for determining luminosity of hydrocarbon fuels
US4963498A (en) * 1985-08-05 1990-10-16 Biotrack Capillary flow device
GB8604328D0 (en) 1986-02-21 1986-03-26 Ici Plc Producing spray of droplets of liquid
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5204112A (en) 1986-06-16 1993-04-20 The Liposome Company, Inc. Induction of asymmetry in vesicles
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US4931225A (en) 1987-12-30 1990-06-05 Union Carbide Industrial Gases Technology Corporation Method and apparatus for dispersing a gas into a liquid
US5185099A (en) * 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5093602A (en) 1989-11-17 1992-03-03 Charged Injection Corporation Methods and apparatus for dispersing a fluent material utilizing an electron beam
US6149789A (en) 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
DE4127405C2 (de) 1991-08-19 1996-02-29 Fraunhofer Ges Forschung Verfahren zur Trennung von Gemischen mikroskopisch kleiner, in einer Flüssigkeit oder einem Gel suspendierter dielektrischer Teilchen und Vorrichtung zur Durchführung des Verfahrens
SE500071C2 (sv) 1992-06-25 1994-04-11 Vattenfall Utveckling Ab Anordning för blandning av två fluider, i synnerhet vätskor med olika temperatur
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
JP2939155B2 (ja) 1994-06-13 1999-08-25 プラクスエア・テクノロジー・インコーポレイテッド 燃焼のための噴霧角度の小さい液体燃料噴霧器
US5935331A (en) 1994-09-09 1999-08-10 Matsushita Electric Industrial Co., Ltd. Apparatus and method for forming films
US5762775A (en) 1994-09-21 1998-06-09 Lockheed Martin Energy Systems, Inc. Method for electrically producing dispersions of a nonconductive fluid in a conductive medium
WO1996039212A2 (en) * 1995-06-06 1996-12-12 Quantic Biomedical Partners Wound sealant preparation and application device and method
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5872010A (en) * 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
JP3759986B2 (ja) 1995-12-07 2006-03-29 フロイント産業株式会社 シームレスカプセルおよびその製造方法
US5681600A (en) 1995-12-18 1997-10-28 Abbott Laboratories Stabilization of liquid nutritional products and method of making
US5868322A (en) 1996-01-31 1999-02-09 Hewlett-Packard Company Apparatus for forming liquid droplets having a mechanically fixed inner microtube
WO1997029508A2 (en) 1996-02-08 1997-08-14 Perseptive Biosystems, Inc. Interface between liquid flow and mass spectrometer
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
WO1997039359A1 (en) * 1996-04-15 1997-10-23 Dade International Inc. Apparatus and method for analysis
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6116516A (en) 1996-05-13 2000-09-12 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
US6196525B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6189803B1 (en) 1996-05-13 2001-02-20 University Of Seville Fuel injection nozzle and method of use
US6386463B1 (en) 1996-05-13 2002-05-14 Universidad De Sevilla Fuel injection nozzle and method of use
US6299145B1 (en) 1996-05-13 2001-10-09 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
ES2140998B1 (es) 1996-05-13 2000-10-16 Univ Sevilla Procedimiento de atomizacion de liquidos.
US6405936B1 (en) 1996-05-13 2002-06-18 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
US6248378B1 (en) 1998-12-16 2001-06-19 Universidad De Sevilla Enhanced food products
US6187214B1 (en) 1996-05-13 2001-02-13 Universidad De Seville Method and device for production of components for microfabrication
CN1173776C (zh) 1996-06-28 2004-11-03 卡钳技术有限公司 在微规模流体性设备里的高通过量的筛选分析系统
JP3361530B2 (ja) * 1996-06-28 2003-01-07 カリパー・テクノロジーズ・コープ． 電子ピペッタおよび電気泳動バイアスのための補償手段
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
DE69707288T2 (de) 1996-07-15 2002-07-18 Calcitech Ltd., Hamilton Herstellung von pulvern
US6252129B1 (en) 1996-07-23 2001-06-26 Electrosols, Ltd. Dispensing device and method for forming material
US6143248A (en) 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
EP0925494B1 (en) 1996-09-04 2001-12-19 Scandinavian Micro Biodevices A/S A micro flow system for particle separation and analysis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
DE19717085C2 (de) * 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
JP2002503336A (ja) 1997-05-16 2002-01-29 アルバータ リサーチ カウンシル 微量流通システムおよびその使用方法
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
JP3081880B2 (ja) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 マイクロスフィアの連続製造装置
US5997636A (en) * 1998-05-01 1999-12-07 Instrumentation Technology Associates, Inc. Method and apparatus for growing crystals
US6003794A (en) 1998-08-04 1999-12-21 Progressive Grower Technologies, Inc. Electrostatic spray module
CN1326549A (zh) 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
US6149787A (en) 1998-10-14 2000-11-21 Caliper Technologies Corp. External material accession systems and methods
US6614598B1 (en) 1998-11-12 2003-09-02 Institute Of Technology, California Microlensing particles and applications
US6450189B1 (en) 1998-11-13 2002-09-17 Universidad De Sevilla Method and device for production of components for microfabrication
US20030069601A1 (en) 1998-12-15 2003-04-10 Closys Corporation Clotting cascade initiating apparatus and methods of use
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US7615373B2 (en) * 1999-02-25 2009-11-10 Virginia Commonwealth University Intellectual Property Foundation Electroprocessed collagen and tissue engineering
US6633031B1 (en) 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
DE19911777A1 (de) 1999-03-17 2000-09-21 Merck Patent Gmbh Verfahren zur Herstellung von kosmetischen Formulierungen
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
WO2000070080A1 (en) 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
EP1192009B1 (en) 1999-06-11 2013-05-01 Aradigm Corporation Method for producing an aerosol
US20060169800A1 (en) 1999-06-11 2006-08-03 Aradigm Corporation Aerosol created by directed flow of fluids and devices and methods for producing same
US6296673B1 (en) * 1999-06-18 2001-10-02 The Regents Of The University Of California Methods and apparatus for performing array microcrystallizations
US6630006B2 (en) * 1999-06-18 2003-10-07 The Regents Of The University Of California Method for screening microcrystallizations for crystal formation
US7244402B2 (en) 2001-04-06 2007-07-17 California Institute Of Technology Microfluidic protein crystallography
US7195670B2 (en) * 2000-06-27 2007-03-27 California Institute Of Technology High throughput screening of crystallization of materials
CN100402850C (zh) 1999-06-28 2008-07-16 加利福尼亚技术学院 微型制造的弹性体的阀和泵系统
KR20020043553A (ko) * 1999-08-12 2002-06-10 죠지 엘. 크레이그 소량 용적의 제어 조작용 마이크로 유체공학 장치
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US20010050881A1 (en) 1999-09-20 2001-12-13 Depaoli David W. Continuous flow, electrohydrodynamic micromixing apparatus and methods
US6890487B1 (en) 1999-09-30 2005-05-10 Science & Technology Corporation ©UNM Flow cytometry for high throughput screening
DE19961257C2 (de) 1999-12-18 2002-12-19 Inst Mikrotechnik Mainz Gmbh Mikrovermischer
WO2001051918A1 (en) * 2000-01-12 2001-07-19 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
AU3499701A (en) * 2000-02-11 2001-08-20 Aclara Biosciences Inc Microfluidic device with sample injector and method
GB2359765B (en) 2000-03-02 2003-03-05 Univ Newcastle Capillary reactor distribution device and method
EP1263687A4 (en) 2000-03-10 2009-11-11 Flow Focusing Inc Method for producing optical fibers by focusing high viscosity liquids
US7485454B1 (en) 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
AU5121801A (en) * 2000-03-31 2001-10-15 Micronics Inc Protein crystallization in microfluidic structures
WO2001077683A1 (en) 2000-04-06 2001-10-18 Caliper Technologies Corp. Methods and devices for achieving long incubation times in high-throughput systems
EP1282910B1 (en) 2000-04-18 2017-04-05 Waters Technologies Corporation Improved electrospray and other lc/ms interfaces
US20010048637A1 (en) 2000-05-24 2001-12-06 Weigl Bernhard H. Microfluidic system and method
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6777450B1 (en) 2000-05-26 2004-08-17 Color Access, Inc. Water-thin emulsions with low emulsifier levels
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
CA2416446A1 (en) 2000-08-04 2002-02-14 Caliper Technologies Corp. Control of operation conditions within fluidic systems
US20040005582A1 (en) * 2000-08-10 2004-01-08 Nanobiodynamics, Incorporated Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
DE10041823C2 (de) 2000-08-25 2002-12-19 Inst Mikrotechnik Mainz Gmbh Verfahren und statischer Mikrovermischer zum Mischen mindestens zweier Fluide
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
WO2002025243A1 (en) 2000-09-21 2002-03-28 Dna Sciences, Inc. Sample injector system and method
US6508988B1 (en) 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US6827095B2 (en) * 2000-10-12 2004-12-07 Nanostream, Inc. Modular microfluidic systems
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
US20040096515A1 (en) 2001-12-07 2004-05-20 Bausch Andreas R. Methods and compositions for encapsulating active agents
EP1385488A2 (en) 2000-12-07 2004-02-04 President And Fellows Of Harvard College Methods and compositions for encapsulating active agents
ES2180405B1 (es) 2001-01-31 2004-01-16 Univ Sevilla Dispositivo y procedimiento para producir chorros liquidos compuestos multicomponentes estacionarios y capsulas multicomponente y/o multicapa de tamaño micro y nanometrico.
TW593122B (en) 2001-02-13 2004-06-21 Qinetiq Ltd Microchannel device
EP1371989A4 (en) 2001-02-23 2006-10-25 Japan Science & Tech Agency METHOD FOR THE TREATMENT OF SMALL LIQUID PARTICLES AND DEVICE THEREFOR
EP1741482B1 (en) 2001-02-23 2008-10-15 Japan Science and Technology Agency Process and apparatus for producing microcapsules
EP1721658B2 (en) 2001-02-23 2020-08-05 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US7037417B2 (en) 2001-03-19 2006-05-02 Ecole Polytechnique Federale De Lausanne Mechanical control of fluids in micro-analytical devices
US20030064414A1 (en) * 2001-03-30 2003-04-03 Benecky Michael J. Rapid assessment of coagulation activity in whole blood
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
WO2002097122A1 (en) 2001-05-26 2002-12-05 One Cell Systems, Inc. Secretion of proteins by encapsulated cells
US6719840B2 (en) * 2001-06-08 2004-04-13 Syrrx, Inc. In situ crystal growth and crystallization
US6797056B2 (en) * 2001-06-08 2004-09-28 Syrrx, Inc. Microfluidic method employing delivery of plural different fluids to same lumen
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
US20030015425A1 (en) 2001-06-20 2003-01-23 Coventor Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
WO2003008102A1 (en) * 2001-07-18 2003-01-30 The Regents Of The University Of Michigan Microfluidic gravity pump with constant flow rate
US6766817B2 (en) * 2001-07-25 2004-07-27 Tubarc Technologies, Llc Fluid conduction utilizing a reversible unsaturated siphon with tubarc porosity action
EP1412065A2 (en) 2001-07-27 2004-04-28 President And Fellows Of Harvard College Laminar mixing apparatus and methods
US6520425B1 (en) 2001-08-21 2003-02-18 The University Of Akron Process and apparatus for the production of nanofibers
US20030089605A1 (en) 2001-10-19 2003-05-15 West Virginia University Research Corporation Microfluidic system for proteome analysis
US7101467B2 (en) * 2002-03-05 2006-09-05 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
EP2497564B1 (en) * 2002-03-05 2014-05-14 Caliper Life Sciences, Inc. Electrophoretic separation in a microfluidic channel network
US7147763B2 (en) 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
EP1508044B1 (en) * 2002-05-09 2010-09-01 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
US7266587B2 (en) * 2002-05-15 2007-09-04 Broadcom Corporation System having interfaces, switch, and memory bridge for CC-NUMA operation
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
GB2395196B (en) 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application
JP2006512092A (ja) * 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア 病原体の検出および分析のための方法および装置
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
EP1605817A2 (en) * 2003-02-25 2005-12-21 Inlight Solutions, Inc. DETERMINATION OF pH INCLUDING HEMOGLOBIN CORRECTION
EP1601874B1 (en) * 2003-03-10 2009-05-27 The Regents Of The University Of Michigan Integrated microfluidic control employing programmable tactile actuators
US7041481B2 (en) * 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7045040B2 (en) 2003-03-20 2006-05-16 Asm Nutool, Inc. Process and system for eliminating gas bubbles during electrochemical processing
EP1473084B1 (en) 2003-03-31 2015-07-29 Canon Kabushiki Kaisha Biochemical reaction cartridge
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
US7262059B2 (en) * 2003-05-06 2007-08-28 Thrombodyne, Inc. Systems and methods for measuring fluid properties
US20060275915A1 (en) 2003-05-16 2006-12-07 Global Technologies (Nz) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
DE112004001376D2 (de) 2003-05-19 2006-04-13 Knoell Hans Forschung Ev Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten
US7115230B2 (en) 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
US20050032238A1 (en) 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
CN1842368B (zh) * 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
US7745221B2 (en) 2003-08-28 2010-06-29 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US7204431B2 (en) 2003-10-31 2007-04-17 Agilent Technologies, Inc. Electrospray ion source for mass spectroscopy
EP1533605A3 (en) 2003-11-19 2006-05-31 Aisin Seiki Kabushiki Kaisha Micro control system for transfer of liquids
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
US7687269B2 (en) * 2003-12-10 2010-03-30 Northeastern University Method for efficient transport of small liquid volumes to, from or within microfluidic devices
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
CN101018816A (zh) 2004-04-23 2007-08-15 尤金妮亚·库马切瓦 生产具有选定尺寸、形状、形态和组成的聚合物颗粒的方法
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7655470B2 (en) * 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
JP5885901B2 (ja) 2004-09-09 2016-03-16 アンスティテュート キュリー マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
KR101544351B1 (ko) * 2005-02-18 2015-08-13 캐논 유.에스. 라이프 사이언시즈, 인크. 유기체의 게놈 ｄｎａ를 확인하기 위한 장치 및 방법
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
FR2882939B1 (fr) 2005-03-11 2007-06-08 Centre Nat Rech Scient Dispositif de separation fluidique
US20070026439A1 (en) * 2005-07-15 2007-02-01 Applera Corporation Fluid processing device and method
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
US7274191B2 (en) * 2005-12-29 2007-09-25 Intel Corporation Integrated on-chip NMR and ESR device and method for making and using the same
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
CN103157400B (zh) * 2007-06-21 2014-12-24 简·探针公司 用于将容器暴露于多个热区的仪器和方法
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2009149257A1 (en) * 2008-06-04 2009-12-10 The University Of Chicago The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
EP4047367A1 (en) * 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8061187B2 (en) * 2009-04-27 2011-11-22 Battelle Memorial Institute Lossless droplet transfer of droplet-based microfluidic analysis
US10434970B2 (en) 2016-12-08 2019-10-08 Toyota Jidosha Kabushiki Kaisha Vehicle side section structure

Also Published As

Publication number Publication date
US20210018941A1 (en) 2021-01-21
WO2006101851A3 (en) 2007-02-22
US10054961B2 (en) 2018-08-21
EP1866092A2 (en) 2007-12-19
US20160187240A1 (en) 2016-06-30
US9696729B2 (en) 2017-07-04
US20170364101A1 (en) 2017-12-21
US20060003439A1 (en) 2006-01-05
EP3763442A1 (en) 2021-01-13
US9477233B2 (en) 2016-10-25
US11853087B2 (en) 2023-12-26
US20140202546A1 (en) 2014-07-24
US10732649B2 (en) 2020-08-04
EP1866092A4 (en) 2010-01-06
EP1866092B1 (en) 2020-04-29
JP2014036956A (ja) 2014-02-27
WO2006101851A2 (en) 2006-09-28

Similar Documents

Publication Publication Date Title
US12097475B2 (en) Microfluidic system
JP5671434B2 (ja) マイクロフルイディックシステム
US11853087B2 (en) Microfluidic system
US11511242B2 (en) Droplet libraries
EP2622103B1 (en) Sandwich assays in droplets
US9637718B2 (en) Methods and devices to control fluid volumes, reagent and particle concentration in arrays of microfluidic drops
Song et al. Reactions in droplets in microfluidic channels
US20050087122A1 (en) Device and method for pressure-driven plug transport and reaction
US20160310947A1 (en) Controlling fluid micro-compartments
WO2005121307A2 (en) Gas control in a microreactor
Lu Continuous microfluidic fabrication and measurement of membrane mechanical properties of synthetic asymmetric vesicles

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140825

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140903

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20141202

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20141205

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20141229

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150202

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150303

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150911

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151211

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160112

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160412

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160614

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160714

R150 Certificate of patent or registration of utility model

Ref document number: 5973407

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees