JP5914576B2 - コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 - Google Patents
コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 Download PDFInfo
- Publication number
- JP5914576B2 JP5914576B2 JP2014118948A JP2014118948A JP5914576B2 JP 5914576 B2 JP5914576 B2 JP 5914576B2 JP 2014118948 A JP2014118948 A JP 2014118948A JP 2014118948 A JP2014118948 A JP 2014118948A JP 5914576 B2 JP5914576 B2 JP 5914576B2
- Authority
- JP
- Japan
- Prior art keywords
- skin
- degradation product
- axillary
- collagen
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000037319 collagen production Effects 0.000 title claims description 27
- 239000002537 cosmetic Substances 0.000 title claims description 24
- 239000000203 mixture Substances 0.000 title description 18
- 102000004190 Enzymes Human genes 0.000 claims description 55
- 108090000790 Enzymes Proteins 0.000 claims description 55
- 229940088598 enzyme Drugs 0.000 claims description 55
- 239000007857 degradation product Substances 0.000 claims description 52
- 210000001099 axilla Anatomy 0.000 claims description 25
- 102000012422 Collagen Type I Human genes 0.000 claims description 18
- 108010022452 Collagen Type I Proteins 0.000 claims description 18
- 230000001737 promoting effect Effects 0.000 claims description 14
- 210000002950 fibroblast Anatomy 0.000 claims description 12
- 230000002500 effect on skin Effects 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 7
- 108010019160 Pancreatin Proteins 0.000 claims description 2
- 229940055695 pancreatin Drugs 0.000 claims description 2
- 210000003491 skin Anatomy 0.000 description 46
- 102000008186 Collagen Human genes 0.000 description 19
- 108010035532 Collagen Proteins 0.000 description 19
- 229920001436 collagen Polymers 0.000 description 19
- 102000004169 proteins and genes Human genes 0.000 description 17
- 108090000623 proteins and genes Proteins 0.000 description 17
- 239000004480 active ingredient Substances 0.000 description 14
- 230000037303 wrinkles Effects 0.000 description 14
- 210000004207 dermis Anatomy 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 230000036558 skin tension Effects 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 239000000284 extract Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000007515 enzymatic degradation Effects 0.000 description 8
- 238000006911 enzymatic reaction Methods 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 241000203242 Collocalia Species 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 239000004365 Protease Substances 0.000 description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 5
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000006210 lotion Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000167880 Hirundinidae Species 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229920002674 hyaluronan Polymers 0.000 description 3
- 229960003160 hyaluronic acid Drugs 0.000 description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000010992 reflux Methods 0.000 description 3
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 150000005846 sugar alcohols Polymers 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- SQVRNKJHWKZAKO-PFQGKNLYSA-N N-acetyl-beta-neuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-PFQGKNLYSA-N 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 229940064004 antiseptic throat preparations Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Chemical compound NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 2
- 239000003906 humectant Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- -1 pH adjusters Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000007665 sagging Methods 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229960002920 sorbitol Drugs 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- TXBCBTDQIULDIA-UHFFFAOYSA-N 2-[[3-hydroxy-2,2-bis(hydroxymethyl)propoxy]methyl]-2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(CO)(CO)COCC(CO)(CO)CO TXBCBTDQIULDIA-UHFFFAOYSA-N 0.000 description 1
- DEENCNYPFDWDSA-UHFFFAOYSA-N 2-ethyl-1,3-benzothiazole-6-sulfonic acid Chemical compound C1=C(S(O)(=O)=O)C=C2SC(CC)=NC2=C1 DEENCNYPFDWDSA-UHFFFAOYSA-N 0.000 description 1
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 241000203223 Aerodramus francicus Species 0.000 description 1
- 241000203221 Aerodramus fuciphagus Species 0.000 description 1
- 241000203250 Aerodramus salangana Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241001237961 Amanita rubescens Species 0.000 description 1
- 241000272876 Apodidae Species 0.000 description 1
- 241000272878 Apodiformes Species 0.000 description 1
- CKLJMWTZIZZHCS-UHFFFAOYSA-N Aspartic acid Chemical compound OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 229940123150 Chelating agent Drugs 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 102000001187 Collagen Type III Human genes 0.000 description 1
- 108010069502 Collagen Type III Proteins 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000489861 Maximus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
- 229940121363 anti-inflammatory agent Drugs 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229960005188 collagen Drugs 0.000 description 1
- 229940096422 collagen type i Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 108010067216 glycyl-glycyl-glycine Proteins 0.000 description 1
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940074358 magnesium ascorbate Drugs 0.000 description 1
- AIOKQVJVNPDJKA-ZZMNMWMASA-L magnesium;(2r)-2-[(1s)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2h-furan-3-olate Chemical compound [Mg+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] AIOKQVJVNPDJKA-ZZMNMWMASA-L 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- WXZMFSXDPGVJKK-UHFFFAOYSA-N pentaerythritol Chemical compound OCC(CO)(CO)CO WXZMFSXDPGVJKK-UHFFFAOYSA-N 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940100515 sorbitan Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003700 vitamin C derivatives Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Images
Description
本発明によるコラーゲン産生促進剤は、前記したように、燕窩の酵素分解物を有効成分とする。
より具体的な例を挙げれば、燕窩の酵素分解物は、以下のようにして調製することができる。
本発明によるコラーゲン産生促進剤は、単独でそのままでも使用することができるが、化粧料、医薬品、医薬部外品などの種々の皮膚外用組成物に添加剤として含有させることができ、コラーゲン産生促進効果を有する組成物を得ることができる。得られた組成物は、スキンケア、好ましくは、肌の張りとツヤの向上、およびシワの防止もしくは改善に有効に使用することができる。
(1-1) 燕窩酵素分解物1
市販の無漂白の燕窩を粉砕機で粉砕して、100メッシュパス(粒径150μm以下)の燕窩粉末を得、この燕窩粉末に、約50倍量(質量)の水を加えて5℃で20時間膨潤させた後、121℃、15分間加熱殺菌処理した。
酵素反応の時間を24時間とした以外は、前記(1-1)項の酵素分解物1と同様に行い、「燕窩酵素分解物2」を得た。
市販の無漂白の燕窩(乾燥物)40gを粉砕機で粉砕し、この粉砕物を、1000mlの蒸留水に加えて、加熱還流下にて2時間抽出処理を行った。加熱還流後、静置した後、上清を分取して濾過し、濾液を回収した。残渣についてはさらに、上記と同様に1000mlの蒸留水に加えて、加熱還流下にて再抽出処理を行った後、固形物残渣を濾過により除去し、濾液を回収した。得られている濾液(抽出液)を併せ、減圧下にて濃縮し、得られた濃縮物を凍結乾燥して、黄白色の「燕窩熱水抽出物」(比較例)を得た。
得られた各燕窩サンプル(燕窩酵素分解物1、燕窩酵素分解物2および燕窩熱水抽出物)中のシアル酸量とタンパク質量とを下記のようにして測定した。
シアル酸の量は、各試料を酸加水分解後に高速液体クロマトグラフィーにて遊離N−アセチルノイラミン酸として測定した。
タンパク質の量は、Brad ford法に基づくBio Rad社のプロテインアッセイキットを使用して測定した。
・カラム: Shodex Asahipak GS320HQ (φ7.6×300mm)
・カラム温度: 35℃
・移動相: 50mM CH3COONH3 pH6.7
・流速: 0.4ml/min
・試料注入量: 10μl
・検出器: 紫外分光検出器(UV220nmにて測定)
[使用した分子量マーカー]
・BSA MW 66,000
・Gly-gly MW 132.1
・Gly-gly-gly MW 189.17
・L-Asparaginic acid MW133.1
・DL-Asparaginic acid MW 150.1
・N-Acetylneuraminic acid MW 309.3
なお、タンパク質の分子量の評価にあたっては、公知文献(Guo, C. T., Suzuki Y., et al., Antiviral Res. 70, pp.140-146 (2006) に示されているデータも参考にした。
正常ヒト皮膚真皮線維芽細胞(KF−4109、クラボウ社より入手)を、0.5%仔牛血清(FBS)含有DMEM培地(SIGMAより入手)を用いて、96穴プレートに2×104cellsの細胞密度にて播種した。播種24時間後、所定の濃度の各燕窩サンプルを含有した0.5%FBS含有DMEM培地と交換し、さらに24時間培養した。
なおポジティブコントロール(P.C.)として、アスコルビン酸リン酸マグネシウム(VC−PMg)を使用した。
培地中のI型コラーゲン量は、全細胞のタンパク質量で培地中のI型コラーゲン量を除することによって単位タンパク質量当たりのI型コラーゲン産生量として算出した。
結果から、燕窩の酵素分解物が、ヒト皮膚真皮線維芽細胞のI型コラーゲン産生を有意に増加させることが認められた。
Claims (5)
- 燕窩をパンクレアチンにより酵素分解することを含む、コラーゲン産生促進外用剤の製造方法。
- 前記酵素分解物の分子量が、70〜200,000である、請求項1に記載の製造方法。
- 前記酵素分解物の平均分子量が、300〜15,000である、請求項1または2に記載の製造方法。
- 前記外用剤が、皮膚真皮繊維芽細胞におけるI型コラーゲン産生を促進するためのものである、請求項1〜3のいずれか一項に記載の製造方法。
- 前記外用剤が化粧料である、請求項1〜4のいずれか一項に記載の製造方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014118948A JP5914576B2 (ja) | 2014-06-09 | 2014-06-09 | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014118948A JP5914576B2 (ja) | 2014-06-09 | 2014-06-09 | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009148754A Division JP5815202B2 (ja) | 2009-06-23 | 2009-06-23 | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2014159490A JP2014159490A (ja) | 2014-09-04 |
JP5914576B2 true JP5914576B2 (ja) | 2016-05-11 |
Family
ID=51611429
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014118948A Active JP5914576B2 (ja) | 2014-06-09 | 2014-06-09 | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5914576B2 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109966234A (zh) * | 2019-05-10 | 2019-07-05 | 西安天久肽健康科技有限公司 | 一种阿胶燕窝肽面膜及其制备方法 |
CN114989244A (zh) * | 2022-08-05 | 2022-09-02 | 广东泰升药业有限公司 | 一种燕窝小分子肽及其制备方法和应用 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2557240B2 (ja) * | 1987-12-21 | 1996-11-27 | 雪印乳業株式会社 | 皮膚老化予防剤 |
JPH0737474B2 (ja) * | 1991-07-05 | 1995-04-26 | 太陽化学株式会社 | 高純度シアル酸の精製法 |
JP3162224B2 (ja) * | 1993-03-15 | 2001-04-25 | 日本碍子株式会社 | シアル酸の調製法 |
JP3449624B2 (ja) * | 1997-11-05 | 2003-09-22 | 株式会社日本ハイポックス | 化粧料および燕窩抽出物の製造方法 |
JP4976629B2 (ja) * | 2001-09-27 | 2012-07-18 | コンビ株式会社 | 美肌促進剤 |
JP4559089B2 (ja) * | 2004-01-23 | 2010-10-06 | コンビ株式会社 | ウイルス捕捉用スプレー剤及びウイルス捕捉フィルター |
GB0411387D0 (en) * | 2004-05-21 | 2004-06-23 | Chiron Srl | Analysis of saccharide length |
JP2006290844A (ja) * | 2005-04-14 | 2006-10-26 | Katakura Chikkarin Co Ltd | 化粧料 |
JP5062980B2 (ja) * | 2005-09-14 | 2012-10-31 | 株式会社ノエビア | 保湿剤、コラーゲン産生促進剤、美白剤、抗酸化剤、及び血管内皮細胞増殖因子産生促進剤 |
JP2008074725A (ja) * | 2006-09-19 | 2008-04-03 | Noevir Co Ltd | 保湿剤、アルギナーゼ活性促進剤、細胞賦活剤、コラーゲン産生促進剤、ヒアルロン酸産生促進剤、抗酸化剤、及び中性脂肪蓄積抑制剤 |
US20100143535A1 (en) * | 2007-07-12 | 2010-06-10 | Hidemasa Motoshima | Method for producing composition containing sialic acid compound |
JP5815202B2 (ja) * | 2009-06-23 | 2015-11-17 | コンビ株式会社 | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 |
JP5815203B2 (ja) * | 2009-06-23 | 2015-11-17 | コンビ株式会社 | コラーゲン産生低下抑制剤、それを含む皮膚外用組成物および化粧料 |
JP5914577B2 (ja) * | 2014-06-09 | 2016-05-11 | コンビ株式会社 | コラーゲン産生低下抑制剤、それを含む皮膚外用組成物および化粧料 |
-
2014
- 2014-06-09 JP JP2014118948A patent/JP5914576B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2014159490A (ja) | 2014-09-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5815202B2 (ja) | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 | |
JP4693623B2 (ja) | 化粧料 | |
JP5588474B2 (ja) | コラーゲンおよび/またはヒアルロン酸産生促進用組成物 | |
JP5815203B2 (ja) | コラーゲン産生低下抑制剤、それを含む皮膚外用組成物および化粧料 | |
KR102085559B1 (ko) | 은이 버섯 추출물, 이의 제조방법 및 이의 용도 | |
JP7213307B2 (ja) | プロテインl-イソアスパラギン酸メチルトランスフェラーゼ活性化用組成物 | |
TWI601540B (zh) | Human Skin Epidermal Cell Proliferator, Compositions Containing Its Skin, and Cosmetic Ingredients | |
JP2008110927A (ja) | ローヤルゼリー蛋白加水分解物を含む化粧料 | |
KR101929657B1 (ko) | 밀배아 발효물의 추출물을 유효성분으로 함유하는 피부 주름 개선 외용제 조성물 | |
JP2008212136A (ja) | 後発酵茶及びその製造方法 | |
JP5914576B2 (ja) | コラーゲン産生促進剤、それを含む皮膚外用組成物および化粧料 | |
JP5914577B2 (ja) | コラーゲン産生低下抑制剤、それを含む皮膚外用組成物および化粧料 | |
JP6951908B2 (ja) | 皮膚外用剤 | |
JP5483928B2 (ja) | 化粧料 | |
KR101677460B1 (ko) | 알레르기성이 감소된 수용성 로열젤리를 포함하는 피부 미백 및 노화방지용 화장료 조성물 | |
JP6704628B2 (ja) | 化粧料 | |
JP2008094799A (ja) | 硫酸化グリコサミノグリカン産生促進剤およびこれを含有する皮膚外用剤 | |
JP2009234976A (ja) | 細胞賦活剤、及び老化防止用皮膚外用剤 | |
JP2009249365A (ja) | 細胞賦活剤、及び老化防止用皮膚外用剤 | |
JP6803113B2 (ja) | 評価方法及び皮膚外用剤 | |
JP2022020422A (ja) | 皮膚外用剤 | |
JP2021031451A (ja) | イチョウ葉由来の発酵組成物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140709 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140709 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150807 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151006 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160304 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160404 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5914576 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |