JP5882234B2 - 核酸ライブラリーの作製方法 - Google Patents

核酸ライブラリーの作製方法 Download PDF

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JP5882234B2
JP5882234B2 JP2012555078A JP2012555078A JP5882234B2 JP 5882234 B2 JP5882234 B2 JP 5882234B2 JP 2012555078 A JP2012555078 A JP 2012555078A JP 2012555078 A JP2012555078 A JP 2012555078A JP 5882234 B2 JP5882234 B2 JP 5882234B2
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droplet
nucleic acid
beads
droplets
sample
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JP2013520202A5 (enExample
JP2013520202A (ja
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ジー ポラック マイケル
ジー ポラック マイケル
イー エックハルト アレン
イー エックハルト アレン
スダルサン アルジュン
スダルサン アルジュン
スリニヴァサン ヴィジェイ
スリニヴァサン ヴィジェイ
ラウズ ジェレミー
ラウズ ジェレミー
イー ウイチョン
イー ウイチョン
トロッタ ニコラス
トロッタ ニコラス
ドペシュワルカル ラーフル
ドペシュワルカル ラーフル
スワー プラサンナ
スワー プラサンナ
エフ スミス グレゴリー
エフ スミス グレゴリー
ノートン スコット
ノートン スコット
リナルツ ピーター
リナルツ ピーター
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Advanced Liquid Logic Inc
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Advanced Liquid Logic Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2012555078A 2010-02-25 2011-02-22 核酸ライブラリーの作製方法 Expired - Fee Related JP5882234B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US30795010P 2010-02-25 2010-02-25
US61/307,950 2010-02-25
US32600010P 2010-04-20 2010-04-20
US61/326,000 2010-04-20
US36751310P 2010-07-26 2010-07-26
US61/367,513 2010-07-26
US41064610P 2010-11-05 2010-11-05
US61/410,646 2010-11-05
PCT/US2011/025711 WO2011106314A2 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries

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JP2013520202A JP2013520202A (ja) 2013-06-06
JP2013520202A5 JP2013520202A5 (enExample) 2014-04-10
JP5882234B2 true JP5882234B2 (ja) 2016-03-09

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JP2016018571A Expired - Fee Related JP6203876B2 (ja) 2010-02-25 2016-02-03 核酸ライブラリーの作製方法

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EP (2) EP2539450B1 (enExample)
JP (2) JP5882234B2 (enExample)
AU (2) AU2011221243B2 (enExample)
CA (1) CA2825984A1 (enExample)
DK (1) DK2539450T3 (enExample)
ES (1) ES2565563T3 (enExample)
HU (1) HUE027972T2 (enExample)
PL (1) PL2539450T3 (enExample)
WO (1) WO2011106314A2 (enExample)

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EP2539450A2 (en) 2013-01-02
AU2011221243B2 (en) 2016-06-02
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ES2565563T3 (es) 2016-04-05
EP3072968A1 (en) 2016-09-28
US20150038344A1 (en) 2015-02-05
US20130225452A1 (en) 2013-08-29
US20130225450A1 (en) 2013-08-29
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US20130203606A1 (en) 2013-08-08
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