HUE027972T2 - A method for generating nucleic acid libraries - Google Patents

A method for generating nucleic acid libraries Download PDF

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Publication number
HUE027972T2
HUE027972T2 HUE11747926A HUE11747926A HUE027972T2 HU E027972 T2 HUE027972 T2 HU E027972T2 HU E11747926 A HUE11747926 A HU E11747926A HU E11747926 A HUE11747926 A HU E11747926A HU E027972 T2 HUE027972 T2 HU E027972T2
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HU
Hungary
Prior art keywords
droplet
nucleic acid
electrodes
beads
sample
Prior art date
Application number
HUE11747926A
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English (en)
Hungarian (hu)
Inventor
Michael G Pollack
Allen E Eckhardt
Arjun Sudarsan
Vijay Srinivasan
Jeremy Rouse
Uichong Yi
Nicholas Trotta
Rahul Dhopeshwarkar
Prasanna Thwar
Gregory F Smith
Scott Norton
Peter Linnartz
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Advanced Liquid Logic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc filed Critical Advanced Liquid Logic Inc
Publication of HUE027972T2 publication Critical patent/HUE027972T2/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HUE11747926A 2010-02-25 2011-02-22 A method for generating nucleic acid libraries HUE027972T2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30795010P 2010-02-25 2010-02-25
US32600010P 2010-04-20 2010-04-20
US36751310P 2010-07-26 2010-07-26
US41064610P 2010-11-05 2010-11-05

Publications (1)

Publication Number Publication Date
HUE027972T2 true HUE027972T2 (en) 2016-11-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
HUE11747926A HUE027972T2 (en) 2010-02-25 2011-02-22 A method for generating nucleic acid libraries

Country Status (10)

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US (4) US20130203606A1 (enExample)
EP (2) EP3072968A1 (enExample)
JP (2) JP5882234B2 (enExample)
AU (2) AU2011221243B2 (enExample)
CA (1) CA2825984A1 (enExample)
DK (1) DK2539450T3 (enExample)
ES (1) ES2565563T3 (enExample)
HU (1) HUE027972T2 (enExample)
PL (1) PL2539450T3 (enExample)
WO (1) WO2011106314A2 (enExample)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP4074838A1 (en) 2010-01-19 2022-10-19 Verinata Health, Inc. Novel protocol for preparing sequencing libraries
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP2567213B1 (en) 2010-05-05 2018-01-24 The Governing Council of the Universtiy of Toronto Method of processing dried samples using digital microfluidic device
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2776165A2 (en) 2011-11-07 2014-09-17 Illumina, Inc. Integrated sequencing apparatuses and methods of use
CN107435070A (zh) * 2012-04-12 2017-12-05 维里纳塔健康公司 拷贝数变异的检测和分类
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) * 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
JP1628116S (enExample) 2012-10-24 2019-04-01
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
BR112015019159A2 (pt) 2013-02-08 2017-07-18 10X Genomics Inc geração de código de barras de polinucleotídeos
EP2970951B1 (en) * 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
EP3409791B1 (en) 2013-03-15 2021-06-30 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014150905A2 (en) 2013-03-15 2014-09-25 Genmark Diagnostics, Inc. Systems, methods, and apparatus for manipulating deformable fluid vessels
WO2015023747A1 (en) * 2013-08-13 2015-02-19 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
AU2014364926B2 (en) 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
JP2017522866A (ja) 2014-06-26 2017-08-17 10エックス ジェノミクス, インコーポレイテッド 核酸配列の分析
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
US10487323B2 (en) 2014-08-01 2019-11-26 The Regents Of The University Of California One-pot multiplex gene synthesis
KR20170066540A (ko) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 액체 중 적어도 하나를 효과적으로 격리시키기 위해 비혼화성 액체를 분리하기 위한 방법 및 디바이스
KR102472027B1 (ko) 2014-10-17 2022-11-30 일루미나 케임브리지 리미티드 근접 보존 전위
US10005080B2 (en) * 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
EP3831481B1 (en) 2014-11-11 2025-06-18 Roche Diagnostics GmbH Fluid sample processing cartridge
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US11634707B2 (en) * 2015-02-10 2023-04-25 Illumina, Inc. Methods and compositions for analyzing cellular components
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
CA2982884C (en) * 2015-04-24 2021-03-30 F. Hoffmann-La Roche Ag Digital pcr systems and methods using digital microfluidics
CN208562324U (zh) 2015-06-05 2019-03-01 米罗库鲁斯公司 空气基质数字微流控(dmf)装置
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
WO2016205233A2 (en) * 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
EP3365108B1 (en) * 2015-10-22 2024-06-12 Illumina, Inc. Filler fluid for fluidic devices
WO2017117440A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Droplet partitioned pcr-based library preparation
AU2016386032B2 (en) * 2016-01-15 2021-08-12 F. Hoffmann-La Roche Ag Deep sequencing profiling of tumors
US10597717B2 (en) 2016-03-22 2020-03-24 Myriad Women's Health, Inc. Combinatorial DNA screening
WO2017193044A1 (en) 2016-05-06 2017-11-09 Counsyl, Inc. Noninvasive prenatal diagnostic
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US20180059056A1 (en) * 2016-08-30 2018-03-01 Sharp Life Science (Eu) Limited Electrowetting on dielectric device including surfactant containing siloxane group
EP3516401B1 (en) 2016-09-19 2025-07-30 Roche Diagnostics GmbH Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3563151A4 (en) 2016-12-28 2020-08-19 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018187476A1 (en) * 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
US10020199B1 (en) * 2017-05-15 2018-07-10 International Business Machines Corporation Porous tin oxide films
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
AU2018288767B2 (en) 2017-06-20 2022-05-26 Illumina, Inc. Nanopore sequencers
CN107217308A (zh) * 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
WO2019023133A1 (en) 2017-07-24 2019-01-31 Miroculus Inc. DIGITAL MICROFLUIDIC SYSTEMS AND METHODS WITH INTEGRATED PLASMA COLLECTION DEVICE
WO2019046860A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USE
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US20190262829A1 (en) * 2018-02-28 2019-08-29 Volta Labs, Inc. Directing Motion of Droplets Using Differential Wetting
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN112469504B (zh) 2018-05-23 2024-08-16 米罗库鲁斯公司 对数字微流控中的蒸发的控制
US12241022B2 (en) 2019-01-04 2025-03-04 Cnpc Usa Corporation Method for making nano- and micro-particles for use as a proppant and fluid-loss-control additive
CN113543883A (zh) 2019-01-31 2021-10-22 米罗库鲁斯公司 非结垢组合物以及用于操控和处理包封的微滴的方法
EP3953041A4 (en) 2019-04-08 2023-01-25 Miroculus Inc. MULTI-CARTRIDGE DIGITAL MICROFLUID DEVICES AND METHODS OF USE
WO2020212588A1 (en) 2019-04-17 2020-10-22 Igenomix, S.L. Non-invasive assay for pre-eclampsia and conditions associated with pre-eclampsia
US20220220564A1 (en) 2019-04-17 2022-07-14 Igenomix, S.L. Improved methods for the early diagnosis of uterine leiomyomas and leiomyosarcomas
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
WO2021118517A1 (en) * 2019-12-09 2021-06-17 Hewlett-Packard Development Company, L.P. Array droplet manipulations
EP4103114A1 (en) * 2020-02-14 2022-12-21 Vestel Elektronik Sanayi ve Ticaret A.S. Cooling system comprising cells for receiving a coolant
US20220401957A1 (en) * 2020-02-24 2022-12-22 Miroculus Inc. Information storage using enzymatic dna synthesis and digital microfluidics
WO2022171318A1 (en) 2021-02-12 2022-08-18 Ipremom Pregnancy Healthcare Diagnostics, S.L. In vitro method for determining the risk of suffering from preeclampsia
EP4301515A4 (en) * 2021-03-02 2025-01-29 Volta Labs, Inc. DROPLET HANDLING METHODS AND SYSTEMS
EP4416305A2 (en) 2021-10-11 2024-08-21 Igenomix S.L. Methods and reagents for the differential diagnosis of uterine tumors
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
WO2024072424A1 (en) * 2022-09-30 2024-04-04 Cnpc Usa Corporation Method for making nano- and micro-particles for use as a proppant and fluid-loss-control additive

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2319175A1 (de) 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
FR2599369B1 (fr) 1986-05-30 1989-03-31 Europ Propulsion Procede de preparation de disilylmethanes
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
EP0852004B1 (en) 1995-10-11 2011-01-19 Luminex Corporation Multiplexed analysis of clinical specimens
WO1999026067A1 (en) 1997-11-18 1999-05-27 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1208382B1 (en) 1999-08-17 2006-04-26 Luminex Corporation Encapsulation of fluorescent particles
ATE412184T1 (de) 1999-08-17 2008-11-15 Luminex Corp Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
IL150020A0 (en) * 2000-10-10 2002-12-01 Diversa Corp High throughput or capillary-based screening for a bioactivity or biomolecule
US7163612B2 (en) 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
WO2003057924A1 (en) * 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
DE10224242A1 (de) * 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513535C (en) * 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
WO2005040228A2 (en) 2003-10-24 2005-05-06 Adhesives Research, Inc. Disintegratable films for diagnostic devices
JP4773360B2 (ja) 2003-11-17 2011-09-14 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 流体を操作するためのシステム
JP2007518991A (ja) 2004-01-14 2007-07-12 ルミネックス・コーポレーション ダイナミックレンジを拡大する方法及びシステム
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
JP5042859B2 (ja) 2005-01-20 2012-10-03 ルミネックス コーポレーション 蛍光ベースの応用で使用される磁性ミクロスフェア
CA2623130C (en) 2005-09-21 2018-01-09 Luminex Corporation Methods and systems for image data processing
US20070207513A1 (en) 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
WO2009140373A2 (en) * 2008-05-13 2009-11-19 Advanced Liquid Logic, Inc. Droplet actuator devices, systems, and methods
ATE490971T1 (de) * 2006-04-18 2010-12-15 Advanced Liquid Logic Inc Biochemie auf tröpfchenbasis
US8128798B2 (en) 2006-07-10 2012-03-06 Hitachi High-Technologies Corporation Liquid transfer device
EP2099930B1 (en) 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
EP2111554B1 (en) * 2007-02-09 2013-05-08 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
JP2010518863A (ja) * 2007-02-23 2010-06-03 ニユー・イングランド・バイオレイブス・インコーポレイテツド インビトロ区画化を用いるタンパク質の選択および富化
CN101679932A (zh) 2007-06-27 2010-03-24 数字化生物系统 用于热交换化学过程的基于数字微流体的装置
US20110303542A1 (en) * 2007-08-08 2011-12-15 Advanced Liquid Logic, Inc. Use of Additives for Enhancing Droplet Operations
EP2191011B1 (en) * 2007-08-29 2017-03-29 Illumina Cambridge Limited Method for sequencing a polynucleotide template
WO2010009365A1 (en) * 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
DK2376517T3 (da) * 2008-10-24 2013-02-11 Epict Technologies Corp Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
WO2010054515A1 (zh) 2008-11-17 2010-05-20 上海医药工业研究院 4-(1-羟基-1-甲基乙基)-2-丙基咪唑-5-羧酸酯的制备方法
WO2010077859A2 (en) * 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator

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AU2016204653B2 (en) 2017-12-14
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WO2011106314A2 (en) 2011-09-01
US20150038344A1 (en) 2015-02-05
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