AU2011221243B2 - Method of making nucleic acid libraries - Google Patents

Method of making nucleic acid libraries Download PDF

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Publication number
AU2011221243B2
AU2011221243B2 AU2011221243A AU2011221243A AU2011221243B2 AU 2011221243 B2 AU2011221243 B2 AU 2011221243B2 AU 2011221243 A AU2011221243 A AU 2011221243A AU 2011221243 A AU2011221243 A AU 2011221243A AU 2011221243 B2 AU2011221243 B2 AU 2011221243B2
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AU
Australia
Prior art keywords
droplet
nucleic acid
electrodes
beads
sample
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Ceased
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AU2011221243A
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AU2011221243A1 (en
Inventor
Rahul Dhopeshwarkar
Allen E. Eckhardt
Peter Linnartz
Scott Norton
Michael G. Pollack
Jeremy Rouse
Gregory F. Smith
Vijay Srinivasan
Arjun Sudarsan
Prasanna Thwar
Nicholas Trotta
Uichong Yi
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Advanced Liquid Logic Inc
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Advanced Liquid Logic Inc
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Publication of AU2011221243A1 publication Critical patent/AU2011221243A1/en
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Publication of AU2011221243B2 publication Critical patent/AU2011221243B2/en
Priority to AU2016204653A priority Critical patent/AU2016204653B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2011221243A 2010-02-25 2011-02-22 Method of making nucleic acid libraries Ceased AU2011221243B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2016204653A AU2016204653B2 (en) 2010-02-25 2016-07-05 Method Of Making Nucleic Acid Libraries

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US30795010P 2010-02-25 2010-02-25
US61/307,950 2010-02-25
US32600010P 2010-04-20 2010-04-20
US61/326,000 2010-04-20
US36751310P 2010-07-26 2010-07-26
US61/367,513 2010-07-26
US41064610P 2010-11-05 2010-11-05
US61/410,646 2010-11-05
PCT/US2011/025711 WO2011106314A2 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2016204653A Division AU2016204653B2 (en) 2010-02-25 2016-07-05 Method Of Making Nucleic Acid Libraries

Publications (2)

Publication Number Publication Date
AU2011221243A1 AU2011221243A1 (en) 2012-10-18
AU2011221243B2 true AU2011221243B2 (en) 2016-06-02

Family

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AU2011221243A Ceased AU2011221243B2 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries
AU2016204653A Ceased AU2016204653B2 (en) 2010-02-25 2016-07-05 Method Of Making Nucleic Acid Libraries

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US (4) US20130203606A1 (enExample)
EP (2) EP2539450B1 (enExample)
JP (2) JP5882234B2 (enExample)
AU (2) AU2011221243B2 (enExample)
CA (1) CA2825984A1 (enExample)
DK (1) DK2539450T3 (enExample)
ES (1) ES2565563T3 (enExample)
HU (1) HUE027972T2 (enExample)
PL (1) PL2539450T3 (enExample)
WO (1) WO2011106314A2 (enExample)

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