CA2825984A1 - Method of making nucleic acid libraries - Google Patents

Method of making nucleic acid libraries Download PDF

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Publication number
CA2825984A1
CA2825984A1 CA2825984A CA2825984A CA2825984A1 CA 2825984 A1 CA2825984 A1 CA 2825984A1 CA 2825984 A CA2825984 A CA 2825984A CA 2825984 A CA2825984 A CA 2825984A CA 2825984 A1 CA2825984 A1 CA 2825984A1
Authority
CA
Canada
Prior art keywords
droplet
nucleic acid
acid fragments
following
beads
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2825984A
Other languages
English (en)
French (fr)
Inventor
Michael G. Pollack
Allen E. Eckhardt
Arjun Sudarsan
Vijay Srinivasan
Jeremy Rouse
Uichong Yi
Nicholas Trotta
Rahul Dhopeshwarkar
Prasanna Thwar
Gregory F. Smith
Scott Norton
Peter Linnartz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Liquid Logic Inc
Original Assignee
Advanced Liquid Logic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc filed Critical Advanced Liquid Logic Inc
Publication of CA2825984A1 publication Critical patent/CA2825984A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2825984A 2010-02-25 2011-02-22 Method of making nucleic acid libraries Abandoned CA2825984A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US30795010P 2010-02-25 2010-02-25
US61/307,950 2010-02-25
US32600010P 2010-04-20 2010-04-20
US61/326,000 2010-04-20
US36751310P 2010-07-26 2010-07-26
US61/367,513 2010-07-26
US41064610P 2010-11-05 2010-11-05
US61/410,646 2010-11-05
PCT/US2011/025711 WO2011106314A2 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries

Publications (1)

Publication Number Publication Date
CA2825984A1 true CA2825984A1 (en) 2011-09-01

Family

ID=44507526

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2825984A Abandoned CA2825984A1 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries

Country Status (10)

Country Link
US (4) US20130203606A1 (enExample)
EP (2) EP3072968A1 (enExample)
JP (2) JP5882234B2 (enExample)
AU (2) AU2011221243B2 (enExample)
CA (1) CA2825984A1 (enExample)
DK (1) DK2539450T3 (enExample)
ES (1) ES2565563T3 (enExample)
HU (1) HUE027972T2 (enExample)
PL (1) PL2539450T3 (enExample)
WO (1) WO2011106314A2 (enExample)

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