JP2013520202A5 - - Google Patents

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JP2013520202A5
JP2013520202A5 JP2012555078A JP2012555078A JP2013520202A5 JP 2013520202 A5 JP2013520202 A5 JP 2013520202A5 JP 2012555078 A JP2012555078 A JP 2012555078A JP 2012555078 A JP2012555078 A JP 2012555078A JP 2013520202 A5 JP2013520202 A5 JP 2013520202A5
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Japan
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nucleic acid
acid fragment
droplet
oil
beads
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JP2012555078A
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JP5882234B2 (ja
JP2013520202A (ja
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Priority claimed from PCT/US2011/025711 external-priority patent/WO2011106314A2/en
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JP2012555078A 2010-02-25 2011-02-22 核酸ライブラリーの作製方法 Expired - Fee Related JP5882234B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US30795010P 2010-02-25 2010-02-25
US61/307,950 2010-02-25
US32600010P 2010-04-20 2010-04-20
US61/326,000 2010-04-20
US36751310P 2010-07-26 2010-07-26
US61/367,513 2010-07-26
US41064610P 2010-11-05 2010-11-05
US61/410,646 2010-11-05
PCT/US2011/025711 WO2011106314A2 (en) 2010-02-25 2011-02-22 Method of making nucleic acid libraries

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JP2016018571A Division JP6203876B2 (ja) 2010-02-25 2016-02-03 核酸ライブラリーの作製方法

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JP2013520202A JP2013520202A (ja) 2013-06-06
JP2013520202A5 true JP2013520202A5 (enExample) 2014-04-10
JP5882234B2 JP5882234B2 (ja) 2016-03-09

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JP2016018571A Expired - Fee Related JP6203876B2 (ja) 2010-02-25 2016-02-03 核酸ライブラリーの作製方法

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US (4) US20130203606A1 (enExample)
EP (2) EP2539450B1 (enExample)
JP (2) JP5882234B2 (enExample)
AU (2) AU2011221243B2 (enExample)
CA (1) CA2825984A1 (enExample)
DK (1) DK2539450T3 (enExample)
ES (1) ES2565563T3 (enExample)
HU (1) HUE027972T2 (enExample)
PL (1) PL2539450T3 (enExample)
WO (1) WO2011106314A2 (enExample)

Families Citing this family (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2363205A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
AU2010343277B2 (en) 2010-01-19 2015-05-28 Verinata Health, Inc. Method for determining copy number variations
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
WO2011137533A1 (en) 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
CN108485940B (zh) * 2012-04-12 2022-01-28 维里纳塔健康公司 拷贝数变异的检测和分类
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) * 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
JP6367196B2 (ja) 2012-08-14 2018-08-01 テンエックス・ジェノミクス・インコーポレイテッド マイクロカプセル組成物および方法
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP3257952B1 (en) 2012-09-11 2020-02-12 Life Technologies Corporation Nucleic acid amplification
EP3427830B1 (en) 2012-10-24 2021-06-23 Genmark Diagnostics Inc. Integrated multiplex target analysis
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
EP3553175B1 (en) * 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
JP6351702B2 (ja) 2013-03-15 2018-07-04 ジェンマーク ダイアグノスティクス， インコーポレイテッド 変形可能流体容器を操作するためのシステム、方法、および装置
US10017807B2 (en) 2013-03-15 2018-07-10 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
EP3033599A4 (en) * 2013-08-13 2017-03-22 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
WO2016019387A1 (en) * 2014-08-01 2016-02-04 The Regents Of The University Of California One-pot multiplex gene synthesis
KR20170066540A (ko) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 액체 중 적어도 하나를 효과적으로 격리시키기 위해 비혼화성 액체를 분리하기 위한 방법 및 디바이스
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
ES3038141T3 (en) 2014-11-11 2025-10-09 Hoffmann La Roche Fluid sample processing cartridge
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
BR112017017025B1 (pt) 2015-02-10 2024-01-02 Illumina, Inc Métodos e composições para analizar componentes celulares
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
CA2982884C (en) * 2015-04-24 2021-03-30 F. Hoffmann-La Roche Ag Digital pcr systems and methods using digital microfluidics
EP3303548A4 (en) 2015-06-05 2019-01-02 Miroculus Inc. Evaporation management in digital microfluidic devices
WO2016197103A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
CN107922941A (zh) * 2015-06-15 2018-04-17 塞弗德公司 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量
SG11201803293SA (en) * 2015-10-22 2018-05-30 Illumina Inc Filler fluid for fluidic devices
CN108430617A (zh) * 2015-12-30 2018-08-21 生物辐射实验室股份有限公司 液滴划分的基于pcr的文库制备
SG11201805600VA (en) * 2016-01-15 2018-07-30 Ventana Med Syst Inc Deep sequencing profiling of tumors
EP3433373B1 (en) 2016-03-22 2022-01-12 Myriad Women's Health, Inc. Combinatorial dna screening
WO2017193044A1 (en) 2016-05-06 2017-11-09 Counsyl, Inc. Noninvasive prenatal diagnostic
CA3034064A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US20180059056A1 (en) * 2016-08-30 2018-03-01 Sharp Life Science (Eu) Limited Electrowetting on dielectric device including surfactant containing siloxane group
US11300578B2 (en) 2016-09-19 2022-04-12 Roche Molecular Systems, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018126082A1 (en) 2016-12-28 2018-07-05 Miroculis Inc. Digital microfluidic devices and methods
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018187476A1 (en) * 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
US10020199B1 (en) * 2017-05-15 2018-07-10 International Business Machines Corporation Porous tin oxide films
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP7272967B2 (ja) * 2017-06-20 2023-05-12 イラミーナ インコーポレーテッド ナノポアシーケンサー
CN107217308A (zh) * 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
EP3658908B1 (en) 2017-07-24 2025-11-12 Integra Biosciences AG Digital microfluidics systems and methods with integrated plasma collection device
EP3676009A4 (en) 2017-09-01 2021-06-16 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USING THEM
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US20190262829A1 (en) 2018-02-28 2019-08-29 Volta Labs, Inc. Directing Motion of Droplets Using Differential Wetting
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CA3096855A1 (en) 2018-05-23 2019-11-28 Miroculus Inc. Control of evaporation in digital microfluidics
US12241022B2 (en) 2019-01-04 2025-03-04 Cnpc Usa Corporation Method for making nano- and micro-particles for use as a proppant and fluid-loss-control additive
US12233390B2 (en) 2019-01-31 2025-02-25 Miroculus Inc. Nonfouling compositions and methods for manipulating and processing encapsulated droplets
WO2020176816A1 (en) 2019-02-28 2020-09-03 Miroculus Inc. Digital microfluidics devices and methods of using them
US11738345B2 (en) 2019-04-08 2023-08-29 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
WO2020212588A1 (en) 2019-04-17 2020-10-22 Igenomix, S.L. Non-invasive assay for pre-eclampsia and conditions associated with pre-eclampsia
EP3956476A1 (en) 2019-04-17 2022-02-23 Igenomix S.L. Improved methods for the early diagnosis of uterine leiomyomas and leiomyosarcomas
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US20220410163A1 (en) * 2019-12-09 2022-12-29 Hewlett-Packard Development Company, L.P. Array droplet manipulations
EP3969611B1 (en) * 2020-02-03 2025-12-10 Illumina, Inc. Biotin-streptavidin cleavage composition and library fragment cleavage
JP2023521535A (ja) * 2020-02-14 2023-05-25 ヴェステル・エレクトロニク・サナイ・ヴェ・ティジャレット・アノニム・シルケティ 冷却剤を受けるセルを含む冷却システム
CA3170835A1 (en) * 2020-02-24 2021-09-02 Miroculus Inc. Information storage using enzymatic dna synthesis and digital microfluidics
JP7813728B2 (ja) 2020-06-12 2026-02-13 エフ． ホフマン－ラ ロシュ アーゲー 診断装置上のベイを評価するためのシステムおよび方法
WO2022171318A1 (en) 2021-02-12 2022-08-18 Ipremom Pregnancy Healthcare Diagnostics, S.L. In vitro method for determining the risk of suffering from preeclampsia
WO2022187381A1 (en) * 2021-03-02 2022-09-09 Volta Labs, Inc. Methods and systems for droplet manipulation
WO2023061914A2 (en) 2021-10-11 2023-04-20 Igenomix, S.L. Methods and reagents for the differential diagnosis of uterine tumors
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
WO2024072424A1 (en) * 2022-09-30 2024-04-04 Cnpc Usa Corporation Method for making nano- and micro-particles for use as a proppant and fluid-loss-control additive

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2319175A1 (de) 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
FR2599369B1 (fr) 1986-05-30 1989-03-31 Europ Propulsion Procede de preparation de disilylmethanes
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
EP0965044B1 (en) 1997-11-18 2003-03-19 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
DE60040603D1 (de) 1999-08-17 2008-12-04 Luminex Corp Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten
DE60027576T2 (de) 1999-08-17 2007-05-03 Luminex Corp., Austin Verkapselung von fluoreszierenden partikeln
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
IL150020A0 (en) * 2000-10-10 2002-12-01 Diversa Corp High throughput or capillary-based screening for a bioactivity or biomolecule
US7163612B2 (en) 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
AU2003207448A1 (en) * 2002-01-04 2003-07-24 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
DE10224242A1 (de) * 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513889A1 (en) * 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
AU2004284080B2 (en) 2003-10-24 2008-10-02 Adhesives Research, Inc. Disintegratable films for diagnostic devices
WO2005047696A1 (en) 2003-11-17 2005-05-26 Koninklijke Philips Electronics N.V. System for manipulation of a body of fluid
CN1910440A (zh) 2004-01-14 2007-02-07 卢米尼克斯股份有限公司 用于动态范围扩展的方法和系统
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
CN101650998B (zh) 2005-01-20 2013-02-06 卢米尼克斯股份有限公司 微球、包含其的微球群、以及分析试验样品的方法
CA2984772C (en) 2005-09-21 2018-04-03 Luminex Corporation Methods and systems for image data processing
WO2007103859A2 (en) 2006-03-03 2007-09-13 Luminex Corporation Methods, products, and kits for identifying an analyte in a sample
WO2009140373A2 (en) * 2008-05-13 2009-11-19 Advanced Liquid Logic, Inc. Droplet actuator devices, systems, and methods
DE602006018794D1 (de) * 2006-04-18 2011-01-20 Advanced Liquid Logic Inc Biochemie auf tröpfchenbasis
WO2008007511A1 (en) 2006-07-10 2008-01-17 Hitachi High-Technologies Corporation Liquid transfer device
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
EP2573562A3 (en) * 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
JP2010518863A (ja) * 2007-02-23 2010-06-03 ニユー・イングランド・バイオレイブス・インコーポレイテツド インビトロ区画化を用いるタンパク質の選択および富化
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
WO2009021173A1 (en) * 2007-08-08 2009-02-12 Advanced Liquid Logic, Inc. Use of additives for enhancing droplet operations
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
WO2010009365A1 (en) * 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
ES2743029T3 (es) * 2008-10-24 2020-02-18 Epicentre Tech Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
EP2374799B1 (en) 2008-11-17 2013-05-22 Shanghai Institute of Pharmaceutical Industry Process for the preparation of 4-(1-hydroxy-1-methylethyl)-2-propyl-imidazole-5-carboxylates
WO2010077859A2 (en) * 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator

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