JP5840855B2 - 胚性幹細胞から心筋シートを製造する方法 - Google Patents

胚性幹細胞から心筋シートを製造する方法 Download PDF

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JP5840855B2
JP5840855B2 JP2011076235A JP2011076235A JP5840855B2 JP 5840855 B2 JP5840855 B2 JP 5840855B2 JP 2011076235 A JP2011076235 A JP 2011076235A JP 2011076235 A JP2011076235 A JP 2011076235A JP 5840855 B2 JP5840855 B2 JP 5840855B2
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cells
sheet
cardiomyocytes
flk
myocardial
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JP2012210156A5 (enExample
JP2012210156A (ja
Inventor
岡野　光夫
光夫 岡野
清水　達也
達也 清水
山下　潤
潤 山下
英利 升本
英利 升本
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Tokyo Womens Medical University
Kyoto University NUC
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Tokyo Womens Medical University
Kyoto University NUC
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Priority to JP2011076235A priority Critical patent/JP5840855B2/ja
Priority to PCT/JP2012/059427 priority patent/WO2012133945A1/ja
Priority to US14/009,018 priority patent/US9623052B2/en
Priority to EP12763936.7A priority patent/EP2692859B1/en
Publication of JP2012210156A publication Critical patent/JP2012210156A/ja
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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JP2011076235A 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法 Expired - Fee Related JP5840855B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2011076235A JP5840855B2 (ja) 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法
PCT/JP2012/059427 WO2012133945A1 (ja) 2011-03-30 2012-03-30 胚性幹細胞から心筋シートを製造する方法
US14/009,018 US9623052B2 (en) 2011-03-30 2012-03-30 Method for producing myocardial sheet from embryonic stem cell
EP12763936.7A EP2692859B1 (en) 2011-03-30 2012-03-30 Method for producing cardiomyocyte sheet from embryonic stem cells

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JP2011076235A JP5840855B2 (ja) 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法

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JP2012210156A JP2012210156A (ja) 2012-11-01
JP2012210156A5 JP2012210156A5 (enExample) 2014-05-15
JP5840855B2 true JP5840855B2 (ja) 2016-01-06

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US (1) US9623052B2 (enExample)
EP (1) EP2692859B1 (enExample)
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Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9598668B2 (en) 2008-10-14 2017-03-21 Cellseed Inc. Temperature-responsive cell culture substrate and method for producing the same
JP5920741B2 (ja) 2012-03-15 2016-05-18 ｉＨｅａｒｔ Ｊａｐａｎ株式会社 人工多能性幹細胞から心筋および血管系混合細胞群を製造する方法
KR101870174B1 (ko) 2013-05-31 2018-06-22 아이하트 재팬 가부시키가이샤 하이드로겔을 포함하는 적층화된 세포 시트
JP6248617B2 (ja) * 2013-12-25 2017-12-20 大日本印刷株式会社 細胞シートの製造方法
EP3138906B1 (en) * 2014-05-01 2019-08-21 iHeart Japan Corporation Cd82-positive cardiac progenitor cells
WO2016076368A1 (ja) * 2014-11-12 2016-05-19 テルモ株式会社 心筋細胞シート
EP3346002B1 (en) * 2015-08-31 2021-09-29 Tokyo Women's Medical University Method for reducing pluripotent stem cells, method for producing cell population having reduced pluripotent stem cells
EP3550013A4 (en) 2016-12-02 2020-06-24 Takara Bio Inc. METHOD FOR THE PRODUCTION OF ENDOTHEL CELLS
JP7344486B2 (ja) 2018-03-30 2023-09-14 国立大学法人京都大学 心筋細胞成熟促進剤
WO2019189553A1 (ja) 2018-03-30 2019-10-03 国立大学法人京都大学 複素環化合物

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
EP2275532B1 (en) 2000-07-21 2012-06-06 Cellseed Inc. Myocardium-like cell sheets and processes for producing them
DK2266500T3 (da) 2003-08-01 2015-04-27 Cellseed Inc Tredimensionel vævstruktur
CN1863904B (zh) 2003-10-03 2014-05-28 福田惠一 由干细胞分化诱导心肌细胞的方法
CN101056973B (zh) 2004-08-27 2011-05-25 第一三共株式会社 以细胞内线粒体为指标的心肌细胞的选择方法
EP1910518B1 (en) 2005-06-22 2019-05-08 Asterias Biotherapeutics, Inc. Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
SG169359A1 (en) 2006-01-31 2011-03-30 Univ Keio A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
WO2007126077A1 (ja) 2006-04-28 2007-11-08 Asubio Pharma Co., Ltd. 多能性幹細胞から心筋細胞を分化誘導する方法
WO2009118928A1 (en) * 2008-03-26 2009-10-01 Kyoto University Efficient production and use of highly cardiogenic progenitors and cardiomyocytes from embryonic and induced pluripotent stem cells
JP5387294B2 (ja) 2009-09-29 2014-01-15 ブラザー工業株式会社 サーバに接続されるプリンタ及びサーバ
JP5654786B2 (ja) 2010-06-28 2015-01-14 株式会社セルシード アクリルアミド誘導体および該誘導体を含む重合体

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WO2012133945A1 (ja) 2012-10-04
US20140056859A1 (en) 2014-02-27
US9623052B2 (en) 2017-04-18
EP2692859A1 (en) 2014-02-05
JP2012210156A (ja) 2012-11-01
EP2692859A4 (en) 2015-01-07
EP2692859B1 (en) 2016-12-28

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