JP5827227B2 - グルコアミラーゼの変異体 - Google Patents

グルコアミラーゼの変異体 Download PDF

Info

Publication number
JP5827227B2
JP5827227B2 JP2012525164A JP2012525164A JP5827227B2 JP 5827227 B2 JP5827227 B2 JP 5827227B2 JP 2012525164 A JP2012525164 A JP 2012525164A JP 2012525164 A JP2012525164 A JP 2012525164A JP 5827227 B2 JP5827227 B2 JP 5827227B2
Authority
JP
Japan
Prior art keywords
glucoamylase
seq
amino acid
starch
parent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012525164A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013502211A (ja
JP2013502211A5 (https=
Inventor
ペーター・エドヴァルド・デウン
リチャード・ボット
カスペル・ウィレム・フルーメン
マルテイン・シルファン・スフェッフェルス
ヴォルフガング・エーレ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International N&H Denmark ApS
Danisco US Inc
Original Assignee
DuPont Nutrition Biosciences ApS
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DuPont Nutrition Biosciences ApS, Danisco US Inc filed Critical DuPont Nutrition Biosciences ApS
Publication of JP2013502211A publication Critical patent/JP2013502211A/ja
Publication of JP2013502211A5 publication Critical patent/JP2013502211A5/ja
Application granted granted Critical
Publication of JP5827227B2 publication Critical patent/JP5827227B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012525164A 2009-08-19 2010-08-18 グルコアミラーゼの変異体 Expired - Fee Related JP5827227B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US23514009P 2009-08-19 2009-08-19
US61/235,140 2009-08-19
US36089110P 2010-07-01 2010-07-01
US61/360,891 2010-07-01
DKPA201070337 2010-07-15
DKPA201070337 2010-07-15
PCT/EP2010/062035 WO2011020852A1 (en) 2009-08-19 2010-08-18 Variants of glucoamylase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015145091A Division JP2016019522A (ja) 2009-08-19 2015-07-22 グルコアミラーゼの変異体

Publications (3)

Publication Number Publication Date
JP2013502211A JP2013502211A (ja) 2013-01-24
JP2013502211A5 JP2013502211A5 (https=) 2014-08-07
JP5827227B2 true JP5827227B2 (ja) 2015-12-02

Family

ID=42797171

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012525164A Expired - Fee Related JP5827227B2 (ja) 2009-08-19 2010-08-18 グルコアミラーゼの変異体

Country Status (12)

Country Link
US (2) US8809023B2 (https=)
EP (1) EP2467474A1 (https=)
JP (1) JP5827227B2 (https=)
CN (4) CN107299123A (https=)
AR (1) AR077942A1 (https=)
AU (1) AU2010284963C1 (https=)
BR (1) BR112012003787A2 (https=)
CA (1) CA2770607C (https=)
EA (1) EA023194B1 (https=)
MX (1) MX2012002032A (https=)
PE (1) PE20121504A1 (https=)
WO (2) WO2011020852A1 (https=)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010284963C1 (en) * 2009-08-19 2014-11-27 Danisco Us Inc. Variants of glucoamylase
WO2012140075A2 (en) * 2011-04-15 2012-10-18 Novozymes A/S Method for production of brewers wort
ES2616917T3 (es) * 2011-12-22 2017-06-14 Dupont Nutrition Biosciences Aps Polipéptidos que tienen actividad de glucoamilasa y método para producirlos
US20150152458A1 (en) 2012-03-28 2015-06-04 Danisco Us Inc. Low temperature method for making high glucose syrup
BR112015003124B1 (pt) * 2012-08-14 2020-12-29 Danisco Us Inc. variantes de glicoamilase de trichoderma reesei, composição enzimática e método de processamento de amido
MX2015002099A (es) * 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
WO2014060474A1 (en) * 2012-10-17 2014-04-24 Novozymes A/S Method for production of brewers wort
EP3027741B1 (en) * 2013-07-29 2019-10-23 Danisco US Inc. Gh61 enzyme variants
WO2015118123A1 (en) 2014-02-07 2015-08-13 Novozymes A/S Compositions for producing glucose syrups
US9982284B2 (en) 2014-02-27 2018-05-29 E I Du Pont De Nemours And Company Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
BR112017011275A2 (pt) 2014-12-01 2018-04-03 Novozymes A/S composição, e, processo para a produção de xarope de glicose.
EP3234166A1 (en) * 2014-12-19 2017-10-25 Danisco US Inc. Glucoamylase blends
CN107250163A (zh) 2015-02-06 2017-10-13 嘉吉公司 修饰的葡糖淀粉酶和具有增强的生物产物产生的酵母菌株
EP3274461A1 (en) 2015-03-27 2018-01-31 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
WO2017106739A1 (en) 2015-12-17 2017-06-22 Cargill, Incorporated Sugar transporter-modified yeast strains and methods for bioproduct production
US9670472B1 (en) 2016-08-05 2017-06-06 Fornia Biosolutions, Inc 2G16 glucoamylase compositions and methods
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
US10227578B2 (en) 2016-08-05 2019-03-12 Fornia Biosolutions, Inc. 2G16 glucoamylase compositions and methods
US10053678B2 (en) 2016-08-05 2018-08-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
RU2733294C1 (ru) 2016-11-18 2020-10-01 Балтика Брюэрис - Парт Оф Зе Карлсберг Груп Способ получения зернового солода и солодовый продукт, полученный таким способом
CN111971379B (zh) * 2018-02-28 2025-03-25 嘉吉公司 葡糖淀粉酶工程化酵母和发酵方法
CA3093378A1 (en) * 2018-03-09 2019-09-12 Danisco Us Inc Glucoamylases and methods of use thereof
EP3869978A4 (en) * 2018-10-22 2022-11-23 DuPont Nutrition Biosciences ApS ENZYMES FOR AN INFUSION PROCESS IN ADDITIONAL BREWERY TECHNICAL AREA
US11535836B2 (en) 2018-12-21 2022-12-27 Fornia Biosolutions, Inc. Variant G6P G7P glucoamylase compositions and methods
US20230167386A1 (en) * 2020-05-21 2023-06-01 Dupont Nutrition Biosciences Aps Uninhibited amylases for brewing with high tannin materials
CN114457058B (zh) * 2021-10-19 2024-05-14 安徽农业大学 一种饲用α淀粉酶的突变改良方法及应用
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins
PL447340A1 (pl) * 2023-12-28 2025-06-30 Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie Zastosowanie preparatu enzymatycznego z Trichoderma reesei oraz kombinacji preparatów enzymatycznych z Trichoderma reesei i Aspergillus niger do zmiany aromatu piwa chmielonego na zimno

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
GB2089836B (en) 1980-12-16 1984-06-20 Suntory Ltd Process for producing alcohol by fermentation without cooking
US4794175A (en) 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US4618579A (en) 1984-09-28 1986-10-21 Genencor, Inc. Raw starch saccharification
JPH0630586B2 (ja) 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
DE3650202T3 (de) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
JPS62272987A (ja) 1986-05-22 1987-11-27 Hitachi Plant Eng & Constr Co Ltd ブドウ糖の製造方法
DE3854256T2 (de) 1987-06-06 1996-02-01 Omnigene Inc Expressionsvektor für hefe.
JPH01191693A (ja) 1988-01-25 1989-08-01 Tdk Corp デンプンの連続的糖化方法
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JP2733330B2 (ja) 1989-08-31 1998-03-30 日本食品化工株式会社 高純度澱粉糖の製造方法
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
CA2038485A1 (en) 1990-03-23 1991-09-24 Donald K. Hadden Nanofiltration process for making dextrose
US5162210A (en) * 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
ES2170748T3 (es) 1990-10-05 2002-08-16 Genencor Int Procedimientos para el tratamiento de tejidos que contienen algodon con celulasa.
ES2322032T3 (es) 1990-12-10 2009-06-16 Genencor Int Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei.
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
US5744716A (en) 1995-06-08 1998-04-28 Scp Global Technologies, A Division Of Preco, Inc. Fluid displacement level, density and concentration measurement system
WO1998003639A1 (en) * 1996-07-24 1998-01-29 Iowa State University Research Foundation, Inc. PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY
US6255084B1 (en) 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
US6352851B1 (en) 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
ATE373703T1 (de) 1998-07-15 2007-10-15 Novozymes As Varianten der glukoamylase
MXPA01000352A (es) * 1998-07-15 2002-06-04 Novozymes As Varientes de glucoamilasa.
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6254914B1 (en) 1999-07-02 2001-07-03 The Board Of Trustees Of The University Of Illinois Process for recovery of corn coarse fiber (pericarp)
CN100510067C (zh) * 1999-07-09 2009-07-08 诺维信公司 葡糖淀粉酶变体
WO2002014490A2 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
EP1432798B1 (en) 2001-08-27 2010-11-17 Syngenta Participations AG Self-processing plants and plant parts
GB0129864D0 (en) 2001-12-13 2002-02-06 Danisco Animal feed
EP1527157A1 (en) * 2002-07-25 2005-05-04 Novozymes A/S Mashing process
US20040219649A1 (en) 2003-03-10 2004-11-04 Novozymes A/S Alcohol product processes
CN102210376B (zh) 2003-03-10 2014-12-31 波伊特研究股份有限公司 利用生淀粉生产乙醇的方法
EP1627049B1 (en) 2003-05-29 2010-02-17 Genencor International, Inc. Novel trichoderma genes
US6899910B2 (en) 2003-06-12 2005-05-31 The United States Of America As Represented By The Secretary Of Agriculture Processes for recovery of corn germ and optionally corn coarse fiber (pericarp)
SI1685244T1 (sl) 2003-11-21 2012-06-29 Danisco Us Inc Ekspresija encimov, ki hidrolizirajo zrnat škrob, v Trichodermi in postopek za proizvajanje glukoze iz zrnatih škrobnih substratov
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
US7037704B2 (en) 2004-05-27 2006-05-02 Genencor International, Inc. Heterologous expression of an Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
CN101068922B (zh) 2004-11-30 2015-06-03 金克克国际有限公司 里氏木霉葡糖淀粉酶及其同源物
EP2363460A3 (en) 2004-12-30 2011-12-28 Genencor International, Inc. Acid fungal proteases
US20080280328A1 (en) * 2005-11-18 2008-11-13 Novozymes A/S Glucoamylase Variants
US7608436B2 (en) * 2006-01-25 2009-10-27 Tate & Lyle Ingredients Americas, Inc. Process for producing saccharide oligomers
EP2061882A2 (en) 2006-10-10 2009-05-27 Danisco US, INC., Genencor Division Glucoamylase variants with altered properties
PL2479267T3 (pl) * 2006-12-21 2017-06-30 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
ES2502743T3 (es) 2007-10-09 2014-10-06 Danisco Us Inc. Variantes de glucoamilasa
ES2527586T3 (es) * 2007-11-20 2015-01-27 Danisco Us Inc. Variantes de glucoamilasa con propiedades modificadas
EP2467475A1 (en) 2009-08-19 2012-06-27 Danisco US Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
AU2010284963C1 (en) * 2009-08-19 2014-11-27 Danisco Us Inc. Variants of glucoamylase

Also Published As

Publication number Publication date
AU2010284963C1 (en) 2014-11-27
US20130309726A9 (en) 2013-11-21
MX2012002032A (es) 2012-03-26
JP2013502211A (ja) 2013-01-24
WO2011020852A1 (en) 2011-02-24
US20120149070A1 (en) 2012-06-14
AR077942A1 (es) 2011-10-05
CA2770607A1 (en) 2011-02-24
CN103119157B (zh) 2018-10-09
US8809023B2 (en) 2014-08-19
CN107299123A (zh) 2017-10-27
PE20121504A1 (es) 2012-11-05
CN102575239B (zh) 2017-06-16
CN103119157A (zh) 2013-05-22
EA201270286A1 (ru) 2013-02-28
WO2012001139A1 (en) 2012-01-05
CN109295032A (zh) 2019-02-01
AU2010284963A1 (en) 2012-03-08
CN102575239A (zh) 2012-07-11
BR112012003787A2 (pt) 2017-07-04
CA2770607C (en) 2019-02-26
US9469848B2 (en) 2016-10-18
WO2012001139A9 (en) 2012-02-23
EP2467474A1 (en) 2012-06-27
EA023194B1 (ru) 2016-05-31
AU2010284963B2 (en) 2014-03-20
US20130102035A1 (en) 2013-04-25

Similar Documents

Publication Publication Date Title
JP5827227B2 (ja) グルコアミラーゼの変異体
CN102776164B (zh) 葡糖淀粉酶变体
US20170306309A1 (en) Variants having glucoamylase activity
CN102712915B (zh) 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
US9677058B2 (en) Polypeptides having glucoamylase activity and method of producing the same
CN104602540B (zh) 能抵抗与氧化相关的活性损失的里氏木霉葡糖淀粉酶变体及其用途
JP2016019522A (ja) グルコアミラーゼの変異体
EP2588602A1 (en) Variants of glucoamylase

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130815

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130815

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20140120

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140623

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20140829

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140909

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141205

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150526

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150722

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150729

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150929

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20151015

R150 Certificate of patent or registration of utility model

Ref document number: 5827227

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees