JP5789513B2 - シナプス小胞サイクリングアッセイおよびシステム - Google Patents
シナプス小胞サイクリングアッセイおよびシステム Download PDFInfo
- Publication number
- JP5789513B2 JP5789513B2 JP2011526037A JP2011526037A JP5789513B2 JP 5789513 B2 JP5789513 B2 JP 5789513B2 JP 2011526037 A JP2011526037 A JP 2011526037A JP 2011526037 A JP2011526037 A JP 2011526037A JP 5789513 B2 JP5789513 B2 JP 5789513B2
- Authority
- JP
- Japan
- Prior art keywords
- synaptic vesicle
- electrode
- synaptic
- wells
- platform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Neurology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Neurosurgery (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Electrochemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9436108P | 2008-09-04 | 2008-09-04 | |
| US61/094,361 | 2008-09-04 | ||
| PCT/US2009/004932 WO2010027446A2 (en) | 2008-09-04 | 2009-08-31 | Synaptic vesicle cycling assays and systems |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015151623A Division JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012501646A JP2012501646A (ja) | 2012-01-26 |
| JP2012501646A5 JP2012501646A5 (cg-RX-API-DMAC7.html) | 2012-10-18 |
| JP5789513B2 true JP5789513B2 (ja) | 2015-10-07 |
Family
ID=41479300
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011526037A Expired - Fee Related JP5789513B2 (ja) | 2008-09-04 | 2009-08-31 | シナプス小胞サイクリングアッセイおよびシステム |
| JP2015151623A Expired - Fee Related JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015151623A Expired - Fee Related JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20120053084A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2329275A2 (cg-RX-API-DMAC7.html) |
| JP (2) | JP5789513B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR20110073494A (cg-RX-API-DMAC7.html) |
| CN (1) | CN102203622A (cg-RX-API-DMAC7.html) |
| AU (2) | AU2009288657A1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2744804C (cg-RX-API-DMAC7.html) |
| WO (1) | WO2010027446A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2254458A1 (en) * | 2008-02-04 | 2010-12-01 | Bayer HealthCare, LLC | Semiconductor based analyte sensors and methods |
| US9217155B2 (en) | 2008-05-28 | 2015-12-22 | University Of Massachusetts | Isolation of novel AAV'S and uses thereof |
| US8734809B2 (en) | 2009-05-28 | 2014-05-27 | University Of Massachusetts | AAV's and uses thereof |
| CN103168236B (zh) | 2010-08-23 | 2016-01-20 | 哈佛大学管理委员会 | 用于膜电位测定的光遗传学探针 |
| US9207237B2 (en) | 2010-08-23 | 2015-12-08 | President And Fellows Of Harvard College | Systems, methods, and workflows for optogenetics analysis |
| US9488637B2 (en) * | 2011-01-26 | 2016-11-08 | The Curators Of The University Of Missouri | Combination of single-cell electroporation and electrical recording using the same electrode |
| CA2870511C (en) | 2011-04-21 | 2023-08-08 | University Of Massachusetts | Raav-based compositions and methods for treating alpha-1 anti-trypsin deficiencies |
| WO2013078347A2 (en) * | 2011-11-23 | 2013-05-30 | President And Fellows Of Harvard College | Systems and methods for imaging at high spatial and/or temporal precision |
| US9696275B2 (en) | 2012-10-12 | 2017-07-04 | The Curators Of The University Of Missouri | Addressable electrode arrays in multiple fluidic compartments and uses thereof |
| EP2913391B1 (en) * | 2012-10-25 | 2020-07-01 | Hamamatsu Photonics K.K. | Cell observation device, and cell observation method |
| US9778189B2 (en) | 2012-10-25 | 2017-10-03 | Hamamatsu Photonics K.K. | Cell observation device, electrical stimulation device, and cell observation method |
| JP6370544B2 (ja) * | 2013-11-11 | 2018-08-08 | 浜松ホトニクス株式会社 | 細胞観察装置及び細胞観察方法 |
| US10415010B2 (en) | 2014-01-16 | 2019-09-17 | Hamamatsu Photonics K.K. | Cell observation device, electrostimulation device, and cell observation method |
| WO2015127128A2 (en) | 2014-02-19 | 2015-08-27 | University Of Massachusetts | Recombinant aavs having useful transcytosis properties |
| EP4410805A3 (en) | 2014-03-18 | 2024-11-27 | University of Massachusetts | Raav-based compositions and methods for treating amyotrophic lateral sclerosis |
| US9594075B2 (en) | 2014-04-22 | 2017-03-14 | Q-State Biosciences, Inc. | Diagnostic methods for neural disorders |
| EP3134522B1 (en) | 2014-04-25 | 2021-10-06 | University of Massachusetts | Recombinant aav vectors useful for reducing immunity against transgene products |
| US9518103B2 (en) | 2014-06-18 | 2016-12-13 | President And Fellows Of Harvard College | Optogenetic probes for measuring membrane potential |
| SG11201701540PA (en) | 2014-08-28 | 2017-03-30 | Stemonix Inc | Method of fabricating cell arrays and uses thereof |
| WO2016054554A1 (en) | 2014-10-03 | 2016-04-07 | University Of Massachusetts | Heterologous targeting peptide grafted aavs |
| JP6842410B2 (ja) | 2014-10-03 | 2021-03-17 | ユニバーシティ オブ マサチューセッツ | 新規の高効率ライブラリーにより同定されるaavベクター |
| RU2738421C2 (ru) | 2014-10-21 | 2020-12-14 | Юниверсити Оф Массачусетс | Варианты рекомбинантных aav и их применения |
| WO2016115333A1 (en) | 2015-01-15 | 2016-07-21 | President And Fellows Of Harvard College | Optical selection of cells |
| EP3256170B1 (en) | 2015-02-13 | 2020-09-23 | University of Massachusetts | Compositions and methods for transient delivery of nucleases |
| US10048275B2 (en) | 2015-03-13 | 2018-08-14 | Q-State Biosciences, Inc. | Cardiotoxicity screening methods |
| CA3019315A1 (en) | 2015-04-23 | 2016-10-27 | University Of Massachusetts | Modulation of aav vector transgene expression |
| US11046955B2 (en) | 2015-04-24 | 2021-06-29 | University Of Massachusetts | Modified AAV constructs and uses thereof |
| US11248212B2 (en) | 2015-06-30 | 2022-02-15 | StemoniX Inc. | Surface energy directed cell self assembly |
| US10760053B2 (en) | 2015-10-15 | 2020-09-01 | StemoniX Inc. | Method of manufacturing or differentiating mammalian pluripotent stem cells or progenitor cells using a hollow fiber bioreactor |
| WO2017070516A1 (en) | 2015-10-22 | 2017-04-27 | University Of Massachusetts | Prostate-targeting adeno-associated virus serotype vectors |
| EP3364997B1 (en) | 2015-10-22 | 2024-01-17 | University of Massachusetts | Aspartoacylase gene therapy in the treatment of canavan disease |
| US11826433B2 (en) | 2016-02-02 | 2023-11-28 | University Of Massachusetts | Method to enhance the efficiency of systemic AAV gene delivery to the central nervous system |
| EP3413928B1 (en) | 2016-02-12 | 2022-04-20 | University of Massachusetts | Anti-angiogenic mirna therapeutics for inhibiting corneal neovascularization |
| EP3225279B1 (en) * | 2016-03-31 | 2023-12-20 | Université de Rennes | Brain tissue stimulation apparatus and computer program |
| US11207426B2 (en) | 2016-04-05 | 2021-12-28 | University Of Massachusetts | Compositions and methods for selective inhibition of grainyhead-like protein expression |
| US11413356B2 (en) | 2016-04-15 | 2022-08-16 | University Of Massachusetts | Methods and compositions for treating metabolic imbalance |
| US11882815B2 (en) | 2016-06-15 | 2024-01-30 | University Of Massachusetts | Recombinant adeno-associated viruses for delivering gene editing molecules to embryonic cells |
| US10457940B2 (en) | 2016-09-22 | 2019-10-29 | University Of Massachusetts | AAV treatment of Huntington's disease |
| WO2018071831A1 (en) | 2016-10-13 | 2018-04-19 | University Of Massachusetts | Aav capsid designs |
| WO2018208972A1 (en) | 2017-05-09 | 2018-11-15 | University Of Massachusetts | Methods of treating amyotrophic lateral sclerosis (als) |
| EP3684937A4 (en) | 2017-09-22 | 2021-06-02 | University of Massachusetts | NEW DUAL SOD1 EXPRESSION VECTORS AND RELATED USES |
| CA3096102A1 (en) | 2018-04-13 | 2019-10-17 | University Of Massachusetts | Bicistronic aav vectors encoding hexosaminidase alpha and beta-subunits and uses thereof |
| JP7065433B2 (ja) * | 2018-08-14 | 2022-05-12 | 日本電信電話株式会社 | 電極及びその製造方法、並びに積層体 |
| JP7709201B2 (ja) | 2018-10-05 | 2025-07-16 | ユニバーシティ オブ マサチューセッツ | GM1ガングリオシドーシスおよびGM2ガングリオシドーシスの処置のためのrAAVベクター |
| AU2020314729B2 (en) * | 2019-07-16 | 2023-07-06 | Meso Scale Technologies, Llc. | Assay apparatuses, methods and reagents |
| JP2024501849A (ja) * | 2020-12-31 | 2024-01-16 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | 光学プレートコントローラおよびリーダ |
| EP4271978A4 (en) | 2020-12-31 | 2024-12-18 | Q-State Biosciences, Inc. | MICROSCOPE WITH SPATIAL IMAGING AND BEAM HOMOGENIZER |
| WO2022146981A1 (en) * | 2020-12-31 | 2022-07-07 | Q-State Biosciences, Inc. | Plate imager |
| EP4271994A4 (en) | 2020-12-31 | 2024-11-27 | Q-State Biosciences, Inc. | Calibrating and normalizing imager for optogenetics |
| JP2024516303A (ja) * | 2021-05-04 | 2024-04-12 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | 薬物フィンガプリンティング |
| WO2023278672A1 (en) | 2021-06-30 | 2023-01-05 | Q-State Biosciences, Inc. | Multiplex optical stimulus and readout |
| JPWO2023210585A1 (cg-RX-API-DMAC7.html) * | 2022-04-25 | 2023-11-02 | ||
| WO2025084395A1 (ja) * | 2023-10-19 | 2025-04-24 | 株式会社Jiksak Bioengineering | 抗シナプトタグミン2抗体 |
| WO2025089346A1 (ja) * | 2023-10-25 | 2025-05-01 | 株式会社Jiksak Bioengineering | 抗シナプトフィジン抗体 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1403645B1 (en) | 1998-07-17 | 2007-09-12 | Vertex Pharmaceuticals (San Diego) LLC | Detector and screening device for ion channels |
| US6686193B2 (en) * | 2000-07-10 | 2004-02-03 | Vertex Pharmaceuticals, Inc. | High throughput method and system for screening candidate compounds for activity against target ion channels |
| US7615356B2 (en) * | 2000-07-10 | 2009-11-10 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
| EP1425065A4 (en) * | 2001-07-12 | 2004-12-15 | Merck & Co Inc | ELECTRIC FIELD STIMULATION WITH EUKARYONTIC CELLS |
| US7221981B2 (en) * | 2002-03-28 | 2007-05-22 | Northstar Neuroscience, Inc. | Electrode geometries for efficient neural stimulation |
| US20070274923A1 (en) | 2005-01-11 | 2007-11-29 | Philippe Brulet | Non-invasive real-time in vivo bioluminescence imaging of local Ca2+ dynamics in living organisms |
-
2009
- 2009-08-31 CN CN2009801440769A patent/CN102203622A/zh active Pending
- 2009-08-31 JP JP2011526037A patent/JP5789513B2/ja not_active Expired - Fee Related
- 2009-08-31 EP EP09789240A patent/EP2329275A2/en not_active Withdrawn
- 2009-08-31 AU AU2009288657A patent/AU2009288657A1/en not_active Abandoned
- 2009-08-31 US US13/062,459 patent/US20120053084A1/en not_active Abandoned
- 2009-08-31 WO PCT/US2009/004932 patent/WO2010027446A2/en not_active Ceased
- 2009-08-31 KR KR1020117007795A patent/KR20110073494A/ko not_active Ceased
- 2009-08-31 CA CA2744804A patent/CA2744804C/en not_active Expired - Fee Related
-
2015
- 2015-07-31 JP JP2015151623A patent/JP6106225B2/ja not_active Expired - Fee Related
-
2016
- 2016-04-15 AU AU2016202387A patent/AU2016202387A1/en not_active Abandoned
-
2018
- 2018-01-11 US US15/868,200 patent/US20180136198A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20120053084A1 (en) | 2012-03-01 |
| WO2010027446A4 (en) | 2010-08-05 |
| EP2329275A2 (en) | 2011-06-08 |
| JP2012501646A (ja) | 2012-01-26 |
| US20180136198A1 (en) | 2018-05-17 |
| CA2744804A1 (en) | 2010-03-11 |
| JP6106225B2 (ja) | 2017-03-29 |
| CN102203622A (zh) | 2011-09-28 |
| WO2010027446A3 (en) | 2010-05-20 |
| AU2009288657A1 (en) | 2010-03-11 |
| CA2744804C (en) | 2019-02-05 |
| KR20110073494A (ko) | 2011-06-29 |
| WO2010027446A2 (en) | 2010-03-11 |
| JP2016019736A (ja) | 2016-02-04 |
| AU2016202387A1 (en) | 2016-05-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6106225B2 (ja) | シナプス小胞サイクリングアッセイおよびシステム | |
| Yoo et al. | Focused ultrasound excites cortical neurons via mechanosensitive calcium accumulation and ion channel amplification | |
| Kohro et al. | Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity | |
| Jing et al. | A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies | |
| Ren et al. | P2X2 subunits contribute to fast synaptic excitation in myenteric neurons of the mouse small intestine | |
| Dong et al. | Unlocking opioid neuropeptide dynamics with genetically encoded biosensors | |
| Hu et al. | Bisphenol A impairs synaptic plasticity by both pre‐and postsynaptic mechanisms | |
| Bollmann et al. | Subcellular topography of visually driven dendritic activity in the vertebrate visual system | |
| EP3362138B1 (en) | Kit for promoting nerve regeneration using electrical stimulation | |
| Park et al. | Function and circuitry of VIP+ interneurons in the mouse retina | |
| US20060018833A1 (en) | Method and system for screening compounds for muscular and/or neurological activity in animals | |
| von Twickel et al. | Individual dopaminergic neurons of lamprey SNc/VTA project to both the striatum and optic tectum but restrict co-release of glutamate to striatum only | |
| Gore et al. | Visualization of plasticity in fear-evoked calcium signals in midbrain dopamine neurons | |
| Allison et al. | Connexin‐36 gap junctions mediate electrical coupling between ventral tegmental area GABA neurons | |
| JP2013240352A (ja) | 発光タンパクによる長期モニタリング方法および解析方法 | |
| US20060063202A1 (en) | High throughput method and system for screening candidate compounds for activity against epilepsy and other neurological diseases | |
| Egashira et al. | Spontaneously recycling synaptic vesicles constitute readily releasable vesicles in intact neuromuscular synapses | |
| US20180149639A1 (en) | Modeling neural network dysfunction | |
| Tian et al. | Unlocking opioid neuropeptide dynamics with genetically-encoded biosensors | |
| MX2012003773A (es) | Genes. metodos y composiciones relacionadas con la neurogenesis y su modulacion. | |
| Aleman et al. | Opposing subclasses of Drosophila ellipsoid body neurons promote and suppress sleep | |
| HK1162671A (en) | Synaptic vesicle cycling assays and systems | |
| WO2003093442A2 (en) | Cell cultures from animal models of alzheimer's disease for screening and testing drug efficacy | |
| JP5153068B2 (ja) | 生物学的相互作用の解析方法 | |
| Colombi et al. | Heterogeneous subpopulations of GABAAR-responding neurons coexist in physiological and pathological mature neuronal networks at increasing scales of complexity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120831 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120831 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20130722 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130820 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20131120 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20131127 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140220 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140826 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20141126 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20141203 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20141226 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150126 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150224 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150602 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150702 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150803 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5789513 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |