CA2744804C - Synaptic vesicle cycling assays and systems - Google Patents

Synaptic vesicle cycling assays and systems Download PDF

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Publication number
CA2744804C
CA2744804C CA2744804A CA2744804A CA2744804C CA 2744804 C CA2744804 C CA 2744804C CA 2744804 A CA2744804 A CA 2744804A CA 2744804 A CA2744804 A CA 2744804A CA 2744804 C CA2744804 C CA 2744804C
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Canada
Prior art keywords
synaptic vesicle
electrode
wells
platform
vesicle cycling
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Expired - Fee Related
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CA2744804A
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English (en)
French (fr)
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CA2744804A1 (en
Inventor
David J. Gerber
Jeffrey R. Cottrell
Timothy A. Ryan
Jonathan M. Levenson
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Galenea Corp
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Galenea Corp
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058Neurological cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/10Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Neurology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Peptides Or Proteins (AREA)
CA2744804A 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems Expired - Fee Related CA2744804C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9436108P 2008-09-04 2008-09-04
US61/094,361 2008-09-04
PCT/US2009/004932 WO2010027446A2 (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Publications (2)

Publication Number Publication Date
CA2744804A1 CA2744804A1 (en) 2010-03-11
CA2744804C true CA2744804C (en) 2019-02-05

Family

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Family Applications (1)

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CA2744804A Expired - Fee Related CA2744804C (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Country Status (8)

Country Link
US (2) US20120053084A1 (cg-RX-API-DMAC7.html)
EP (1) EP2329275A2 (cg-RX-API-DMAC7.html)
JP (2) JP5789513B2 (cg-RX-API-DMAC7.html)
KR (1) KR20110073494A (cg-RX-API-DMAC7.html)
CN (1) CN102203622A (cg-RX-API-DMAC7.html)
AU (2) AU2009288657A1 (cg-RX-API-DMAC7.html)
CA (1) CA2744804C (cg-RX-API-DMAC7.html)
WO (1) WO2010027446A2 (cg-RX-API-DMAC7.html)

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US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
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WO2013078347A2 (en) * 2011-11-23 2013-05-30 President And Fellows Of Harvard College Systems and methods for imaging at high spatial and/or temporal precision
US9696275B2 (en) 2012-10-12 2017-07-04 The Curators Of The University Of Missouri Addressable electrode arrays in multiple fluidic compartments and uses thereof
EP2913391B1 (en) * 2012-10-25 2020-07-01 Hamamatsu Photonics K.K. Cell observation device, and cell observation method
US9778189B2 (en) 2012-10-25 2017-10-03 Hamamatsu Photonics K.K. Cell observation device, electrical stimulation device, and cell observation method
JP6370544B2 (ja) * 2013-11-11 2018-08-08 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
US10415010B2 (en) 2014-01-16 2019-09-17 Hamamatsu Photonics K.K. Cell observation device, electrostimulation device, and cell observation method
WO2015127128A2 (en) 2014-02-19 2015-08-27 University Of Massachusetts Recombinant aavs having useful transcytosis properties
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US9594075B2 (en) 2014-04-22 2017-03-14 Q-State Biosciences, Inc. Diagnostic methods for neural disorders
EP3134522B1 (en) 2014-04-25 2021-10-06 University of Massachusetts Recombinant aav vectors useful for reducing immunity against transgene products
US9518103B2 (en) 2014-06-18 2016-12-13 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
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WO2016054554A1 (en) 2014-10-03 2016-04-07 University Of Massachusetts Heterologous targeting peptide grafted aavs
JP6842410B2 (ja) 2014-10-03 2021-03-17 ユニバーシティ オブ マサチューセッツ 新規の高効率ライブラリーにより同定されるaavベクター
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WO2016115333A1 (en) 2015-01-15 2016-07-21 President And Fellows Of Harvard College Optical selection of cells
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US11046955B2 (en) 2015-04-24 2021-06-29 University Of Massachusetts Modified AAV constructs and uses thereof
US11248212B2 (en) 2015-06-30 2022-02-15 StemoniX Inc. Surface energy directed cell self assembly
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WO2017070516A1 (en) 2015-10-22 2017-04-27 University Of Massachusetts Prostate-targeting adeno-associated virus serotype vectors
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US11826433B2 (en) 2016-02-02 2023-11-28 University Of Massachusetts Method to enhance the efficiency of systemic AAV gene delivery to the central nervous system
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Also Published As

Publication number Publication date
US20120053084A1 (en) 2012-03-01
WO2010027446A4 (en) 2010-08-05
EP2329275A2 (en) 2011-06-08
JP2012501646A (ja) 2012-01-26
US20180136198A1 (en) 2018-05-17
CA2744804A1 (en) 2010-03-11
JP6106225B2 (ja) 2017-03-29
CN102203622A (zh) 2011-09-28
WO2010027446A3 (en) 2010-05-20
AU2009288657A1 (en) 2010-03-11
KR20110073494A (ko) 2011-06-29
WO2010027446A2 (en) 2010-03-11
JP2016019736A (ja) 2016-02-04
JP5789513B2 (ja) 2015-10-07
AU2016202387A1 (en) 2016-05-05

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