JP5731113B2 - 反応化合物と安定化ポリメラーゼの乾燥組成物 - Google Patents

反応化合物と安定化ポリメラーゼの乾燥組成物 Download PDF

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JP5731113B2
JP5731113B2 JP2009286615A JP2009286615A JP5731113B2 JP 5731113 B2 JP5731113 B2 JP 5731113B2 JP 2009286615 A JP2009286615 A JP 2009286615A JP 2009286615 A JP2009286615 A JP 2009286615A JP 5731113 B2 JP5731113 B2 JP 5731113B2
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dry composition
aptamer
liquid mixture
polymerase
reaction
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JP2010142229A5 (cg-RX-API-DMAC10.html
JP2010142229A (ja
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ハスリンガー トビアス
ハスリンガー トビアス
メッツラー トーマス
メッツラー トーマス
ペツェニー アンネット
ペツェニー アンネット
ゾベク ハーラルト
ゾベク ハーラルト
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2009286615A 2008-12-19 2009-12-17 反応化合物と安定化ポリメラーゼの乾燥組成物 Active JP5731113B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08022082.5 2008-12-19
EP08022082 2008-12-19

Related Child Applications (1)

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JP2015003548A Division JP2015091261A (ja) 2008-12-19 2015-01-09 反応化合物と安定化ポリメラーゼの乾燥組成物

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JP2010142229A JP2010142229A (ja) 2010-07-01
JP2010142229A5 JP2010142229A5 (cg-RX-API-DMAC10.html) 2012-01-26
JP5731113B2 true JP5731113B2 (ja) 2015-06-10

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JP2009286615A Active JP5731113B2 (ja) 2008-12-19 2009-12-17 反応化合物と安定化ポリメラーゼの乾燥組成物
JP2015003548A Pending JP2015091261A (ja) 2008-12-19 2015-01-09 反応化合物と安定化ポリメラーゼの乾燥組成物

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US (3) US8652811B2 (cg-RX-API-DMAC10.html)
EP (1) EP2202302B1 (cg-RX-API-DMAC10.html)
JP (2) JP5731113B2 (cg-RX-API-DMAC10.html)
CN (1) CN101768616B (cg-RX-API-DMAC10.html)
CA (1) CA2688174C (cg-RX-API-DMAC10.html)
ES (1) ES2394754T3 (cg-RX-API-DMAC10.html)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013527769A (ja) * 2010-05-06 2013-07-04 アイビス バイオサイエンシズ インコーポレイティッド 統合された試料調製システムおよび安定化酵素混合物
EP2598661B1 (en) 2010-07-26 2017-09-27 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
CA2810291C (en) 2010-10-04 2016-09-27 F. Hoffmann-La Roche Ag Method for cell lysis and amplification of rna in a rt-pcr reaction buffer
WO2012045669A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis and pcr within the same reaction vessel
JP2013005796A (ja) * 2011-05-26 2013-01-10 Arkray Inc 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法
EP2574931B1 (en) 2011-09-29 2017-03-22 Qiagen GmbH Dry composition comprising a control dye
EP3663408B1 (en) * 2011-10-11 2024-05-01 QIAGEN GmbH Sample processing method and sample processing cartridge
EP2776577B1 (en) 2011-11-07 2017-01-11 Qiagen GmbH Lysis method and lysis composition
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
EP3008177B1 (en) 2013-06-11 2020-06-03 Biocartis NV Biomolecule drying process for long-term storage
US10036061B2 (en) * 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
EP3015553A1 (de) * 2014-10-30 2016-05-04 Biotecon Diagnostics GmbH Stabilisierte Reaktionsmischung
ES2946184T3 (es) 2015-12-08 2023-07-13 Biomatrica Inc Reducción de la velocidad de eritrosedimentación
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109486824B (zh) * 2018-11-20 2021-11-16 安徽省昂普拓迈生物科技有限责任公司 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用
CN111961709A (zh) * 2020-01-21 2020-11-20 上海酷乐生物科技有限公司 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒
AU2022270084B2 (en) 2021-05-03 2025-03-06 Co-Diagnostics, Inc Systems, methods, and apparatus for automated self-contained biological analysis
JPWO2024106507A1 (cg-RX-API-DMAC10.html) * 2022-11-18 2024-05-23
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3300474A (en) 1964-02-12 1967-01-24 Pharmacia Ab Sucrose ether copolymerizates
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
GB8826429D0 (en) 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
ATE318832T1 (de) 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5565318A (en) 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
US5593824A (en) 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
EP1442794B1 (en) 1996-06-04 2006-03-29 University Of Utah Research Foundation System and method for carrying out and monitoring biological processes
EP1002875A1 (en) * 1998-11-17 2000-05-24 Mira Diagnostica GmbH An oligonucleotide inhibitor of thermostable polymerase
CA2392009C (en) 1999-11-17 2009-07-14 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
EP1466018B2 (en) 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients
JP5254949B2 (ja) * 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
EP2210955A3 (en) * 2006-05-23 2010-09-15 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
EP2063866B2 (en) 2006-09-18 2017-08-23 GE Healthcare Bio-Sciences Corp. Preparation of glassified biological reagents
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
WO2008155529A1 (en) * 2007-06-16 2008-12-24 Enigma Diagnostics Limited Compositions

Also Published As

Publication number Publication date
CA2688174C (en) 2018-08-07
EP2202302A1 (en) 2010-06-30
CN101768616A (zh) 2010-07-07
US20100159529A1 (en) 2010-06-24
US9631233B2 (en) 2017-04-25
CN101768616B (zh) 2014-07-09
CA2688174A1 (en) 2010-06-19
ES2394754T3 (es) 2013-02-05
EP2202302B1 (en) 2012-09-12
US8652811B2 (en) 2014-02-18
JP2010142229A (ja) 2010-07-01
US9163286B2 (en) 2015-10-20
JP2015091261A (ja) 2015-05-14
US20160237473A1 (en) 2016-08-18
US20140162267A1 (en) 2014-06-12

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