JP5566188B2 - 生体観察装置 - Google Patents

生体観察装置 Download PDF

Info

Publication number
JP5566188B2
JP5566188B2 JP2010122846A JP2010122846A JP5566188B2 JP 5566188 B2 JP5566188 B2 JP 5566188B2 JP 2010122846 A JP2010122846 A JP 2010122846A JP 2010122846 A JP2010122846 A JP 2010122846A JP 5566188 B2 JP5566188 B2 JP 5566188B2
Authority
JP
Japan
Prior art keywords
biological sample
image
micro
observation
macro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2010122846A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011008245A (ja
JP2011008245A5 (enExample
Inventor
博之 木村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to JP2010122846A priority Critical patent/JP5566188B2/ja
Publication of JP2011008245A publication Critical patent/JP2011008245A/ja
Publication of JP2011008245A5 publication Critical patent/JP2011008245A5/ja
Application granted granted Critical
Publication of JP5566188B2 publication Critical patent/JP5566188B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2010122846A 2009-05-29 2010-05-28 生体観察装置 Expired - Fee Related JP5566188B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2010122846A JP5566188B2 (ja) 2009-05-29 2010-05-28 生体観察装置

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009130880 2009-05-29
JP2009130880 2009-05-29
JP2010122846A JP5566188B2 (ja) 2009-05-29 2010-05-28 生体観察装置

Publications (3)

Publication Number Publication Date
JP2011008245A JP2011008245A (ja) 2011-01-13
JP2011008245A5 JP2011008245A5 (enExample) 2014-03-06
JP5566188B2 true JP5566188B2 (ja) 2014-08-06

Family

ID=42729405

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010122846A Expired - Fee Related JP5566188B2 (ja) 2009-05-29 2010-05-28 生体観察装置

Country Status (3)

Country Link
US (1) US9810895B2 (enExample)
EP (1) EP2256534B1 (enExample)
JP (1) JP5566188B2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9310598B2 (en) 2009-03-11 2016-04-12 Sakura Finetek U.S.A., Inc. Autofocus method and autofocus device
US10139613B2 (en) * 2010-08-20 2018-11-27 Sakura Finetek U.S.A., Inc. Digital microscope and method of sensing an image of a tissue sample
JP2012118448A (ja) * 2010-12-03 2012-06-21 Sony Corp 画像処理方法、画像処理装置及び画像処理プログラム
JP5887766B2 (ja) * 2011-08-31 2016-03-16 株式会社ニコン 顕微鏡制御装置、画像処理装置、顕微鏡装置およびプログラム
JP5761061B2 (ja) * 2012-02-08 2015-08-12 株式会社島津製作所 撮像装置及び顕微鏡、並びに、これらに用いられるプログラム
JP6061614B2 (ja) * 2012-10-25 2017-01-18 オリンパス株式会社 顕微鏡システム
DE102012022603B3 (de) * 2012-11-19 2014-05-08 Acquifer Ag Vorrichtung und Verfahren zur Mikroskopie einer Vielzahl von Proben
JP6278601B2 (ja) * 2013-02-15 2018-02-14 オリンパス株式会社 顕微鏡システム及びプログラム
JP6455829B2 (ja) * 2013-04-01 2019-01-23 キヤノン株式会社 画像処理装置、画像処理方法、およびプログラム
DE102013103971A1 (de) 2013-04-19 2014-11-06 Sensovation Ag Verfahren zum Erzeugen eines aus mehreren Teilbildern zusammengesetzten Gesamtbilds eines Objekts
JP6177000B2 (ja) * 2013-05-15 2017-08-09 オリンパス株式会社 レーザ走査型顕微鏡
JP6215677B2 (ja) * 2013-12-05 2017-10-18 株式会社日立ハイテクマニファクチャ＆サービス 顕微ラマン分光装置および顕微ラマン分光システム
US10007102B2 (en) 2013-12-23 2018-06-26 Sakura Finetek U.S.A., Inc. Microscope with slide clamping assembly
JP2015127738A (ja) * 2013-12-27 2015-07-09 ソニー株式会社 顕微鏡システムおよびその制御方法
JP6173950B2 (ja) * 2014-03-04 2017-08-02 富士フイルム株式会社 細胞撮像制御装置および方法並びにプログラム
CN105067520B (zh) * 2015-07-28 2018-10-23 爱威科技股份有限公司 一种镜检识别方法及装置
CN106023225B (zh) * 2016-05-30 2019-03-08 武汉沃亿生物有限公司 生物样本显微成像的自动修改成像区间方法
JP2018000141A (ja) * 2016-07-06 2018-01-11 オリンパス株式会社 観察システム及び観察方法
EP3538940A1 (en) * 2016-11-12 2019-09-18 The Trustees of Columbia University in the City of New York Microscopy devices, methods and systems
US11280803B2 (en) * 2016-11-22 2022-03-22 Sakura Finetek U.S.A., Inc. Slide management system
JP6410902B2 (ja) * 2017-09-04 2018-10-24 株式会社日立ハイテクマニファクチャ＆サービス 顕微ラマン分光装置および顕微ラマン分光システム
DE102018128281B3 (de) * 2018-11-12 2019-11-14 Leica Microsystems Cms Gmbh Mikroskopsystem und Verfahren zur Untersuchung einer Probe
US11543639B2 (en) * 2019-01-29 2023-01-03 Li-Cor, Inc. Macro-micro telecentric scanning systems and methods
WO2021166219A1 (ja) * 2020-02-21 2021-08-26 オリンパス株式会社 光学顕微鏡システム
JP2023161621A (ja) * 2022-04-26 2023-11-08 株式会社ニコン 顕微鏡

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1096848A (ja) 1996-09-20 1998-04-14 Olympus Optical Co Ltd 自動焦点検出装置
US6711283B1 (en) 2000-05-03 2004-03-23 Aperio Technologies, Inc. Fully automatic rapid microscope slide scanner
GB2398196B (en) * 2003-02-05 2005-06-01 Fairfield Imaging Ltd Microscope system and method
CA2522543A1 (en) * 2003-04-14 2004-10-28 Surface Logix, Inc. Devices and assays for monitoring/measuring cellular dynamics to create subject profiles from primary cells
US7532323B2 (en) * 2003-10-28 2009-05-12 Cha-Min Tang Spatial light modulator apparatus and method
US7227113B2 (en) 2003-11-21 2007-06-05 Olympus Corporation Confocal laser scanning microscope
JP2006003653A (ja) * 2004-06-17 2006-01-05 Olympus Corp 生体試料観察システム
US7355702B2 (en) 2004-06-21 2008-04-08 Olympus Corporation Confocal observation system
JP4700299B2 (ja) 2004-07-07 2011-06-15 オリンパス株式会社 共焦点走査型顕微鏡
JP2006017489A (ja) 2004-06-30 2006-01-19 Nikon Corp 細胞識別装置、細胞識別用プログラム、細胞解析装置、および細胞解析用プログラム
JP4600395B2 (ja) 2004-09-22 2010-12-15 株式会社ニコン 顕微鏡システムおよび画像処理方法
JP2006209698A (ja) 2005-01-31 2006-08-10 Olympus Corp 対象追跡装置、顕微鏡システムおよび対象追跡プログラム
WO2006123641A1 (ja) 2005-05-16 2006-11-23 Olympus Corporation 走査型観察装置
US7864996B2 (en) 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
US7643209B2 (en) * 2006-08-02 2010-01-05 Olympus Corporation Microscope optical system, microscope apparatus, and microscope observation method
EP2711681A1 (en) * 2006-09-21 2014-03-26 Kurashiki Boseki Kabushiki Kaisha Apparatus and method for preparing sliced specimen
DE602006011913D1 (de) 2006-10-31 2010-03-11 Olympus Corp Mikroskopsystem, Beobachtungsverfahren und Beobachtungsprogramm
JP4474655B2 (ja) 2007-05-09 2010-06-09 独立行政法人科学技術振興機構 顕微鏡装置
WO2008153836A2 (en) 2007-05-31 2008-12-18 President And Fellows Of Harvard College Target-locking acquisition with real-time confocal (tarc) microscopy
EP2213722B1 (en) * 2007-10-19 2018-01-10 Nikon Corporation Program, computer, and culture state analyzing method
JP5268324B2 (ja) * 2007-10-29 2013-08-21 株式会社日立ハイテクノロジーズ 荷電粒子線顕微装置及び顕微方法
JP2009152827A (ja) * 2007-12-20 2009-07-09 Nikon Corp タイムラプス画像の画像処理方法、画像処理プログラム及び画像処理装置
JP4953092B2 (ja) * 2008-03-04 2012-06-13 株式会社ニコン 細胞観察における生細胞の判別手法、細胞観察の画像処理プログラム及び画像処理装置

Also Published As

Publication number Publication date
US20100310139A1 (en) 2010-12-09
EP2256534B1 (en) 2017-03-29
JP2011008245A (ja) 2011-01-13
US9810895B2 (en) 2017-11-07
EP2256534A1 (en) 2010-12-01

Similar Documents

Publication Publication Date Title
JP5566188B2 (ja) 生体観察装置
JP6158197B2 (ja) 自動化された顕微鏡使用のための多関数型自動焦点システムおよび方法
US20140313312A1 (en) Digital microscope and method for optimizing the work process in a digital microscope
US11226478B2 (en) Microscope and method for viewing a specimen using a microscope
EP2758825B1 (en) Slide scanner with a tilted image plane
US9122070B2 (en) Microscope device
US20150168706A1 (en) Microscope and Method for SPIM Microscopy
JP2005284136A (ja) 観察装置および観察装置の焦点合わせ方法
JP2014078008A (ja) レーザー顕微解剖領域の画定方法及び関連するレーザー顕微解剖システム
JP2010286566A (ja) レーザ走査型蛍光顕微鏡および蛍光観察方法
JP2015127738A (ja) 顕微鏡システムおよびその制御方法
JP6548965B2 (ja) 顕微鏡システムおよび顕微鏡観察方法
US20180180867A1 (en) Microscope and setting support method
JP2010286565A (ja) 蛍光観察装置
JP6161399B2 (ja) 顕微鏡システム
JP2007114742A (ja) 観察装置
JP5471715B2 (ja) 合焦装置、合焦方法、合焦プログラム及び顕微鏡
US20180039059A1 (en) Microscope system
JP7134839B2 (ja) 顕微鏡装置、制御方法、及び、プログラム
JP2018194634A (ja) ライトフィールド顕微鏡
US8913819B2 (en) Observation apparatus and observation method
JP5019279B2 (ja) 共焦点顕微鏡及び合焦カラー画像の生成方法
JP5649994B2 (ja) 顕微鏡装置
JP2014186332A (ja) 蛍光観察装置
JP2010164635A (ja) 共焦点顕微鏡

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130510

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20140117

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140225

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140425

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140610

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140617

R151 Written notification of patent or utility model registration

Ref document number: 5566188

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R151

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

R371 Transfer withdrawn

Free format text: JAPANESE INTERMEDIATE CODE: R371

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

R371 Transfer withdrawn

Free format text: JAPANESE INTERMEDIATE CODE: R371

LAPS Cancellation because of no payment of annual fees