JP5518834B2 - 生体試料の反復染色 - Google Patents
生体試料の反復染色 Download PDFInfo
- Publication number
- JP5518834B2 JP5518834B2 JP2011503120A JP2011503120A JP5518834B2 JP 5518834 B2 JP5518834 B2 JP 5518834B2 JP 2011503120 A JP2011503120 A JP 2011503120A JP 2011503120 A JP2011503120 A JP 2011503120A JP 5518834 B2 JP5518834 B2 JP 5518834B2
- Authority
- JP
- Japan
- Prior art keywords
- flow cell
- premixer
- flow
- reagent
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/061,044 | 2008-04-02 | ||
| US12/061,044 US20090253163A1 (en) | 2008-04-02 | 2008-04-02 | Iterative staining of biological samples |
| PCT/US2009/039052 WO2009124099A1 (en) | 2008-04-02 | 2009-04-01 | Iterative staining of biological samples |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011518320A JP2011518320A (ja) | 2011-06-23 |
| JP2011518320A5 JP2011518320A5 (enExample) | 2014-03-13 |
| JP5518834B2 true JP5518834B2 (ja) | 2014-06-11 |
Family
ID=41133623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011503120A Expired - Fee Related JP5518834B2 (ja) | 2008-04-02 | 2009-04-01 | 生体試料の反復染色 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20090253163A1 (enExample) |
| EP (1) | EP2260283A1 (enExample) |
| JP (1) | JP5518834B2 (enExample) |
| CN (1) | CN101983327A (enExample) |
| WO (1) | WO2009124099A1 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090249949A1 (en) * | 2008-03-05 | 2009-10-08 | Helicos Biosciences Corporation | Methods and devices for bubble mitigation |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US8673643B2 (en) | 2010-11-30 | 2014-03-18 | General Electric Company | Closed loop monitoring of automated molecular pathology system |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9150907B2 (en) | 2012-04-27 | 2015-10-06 | General Electric Company | Microfluidic flow cell assemblies and method of use |
| US9164015B2 (en) * | 2012-06-29 | 2015-10-20 | General Electric Company | Systems and methods for processing and imaging of biological samples |
| US20140055853A1 (en) * | 2012-08-27 | 2014-02-27 | General Electric Company | Open top microfluidic device for multiplexing |
| ES2969390T3 (es) * | 2012-10-08 | 2024-05-17 | Ventana Med Syst Inc | Procedimientos y kits para clarificar muestras pigmentadas |
| EP3901280B1 (en) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP2932270B1 (en) | 2012-12-11 | 2018-10-24 | GE Healthcare Bio-Sciences Corp. | Photoactivated chemical bleaching of dyes |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| US20150198580A1 (en) | 2014-01-13 | 2015-07-16 | General Electric Company | Coverslip and methods for removing |
| CN103743611A (zh) * | 2014-01-16 | 2014-04-23 | 西南大学 | 一种烟草腺毛及腺毛分泌物的染色液和染色方法 |
| ES2918325T3 (es) * | 2014-04-30 | 2022-07-15 | Ventana Med Syst Inc | Procedimiento de limpieza para precipitados de hemateína en aparatos de tinción automatizados |
| US10168259B2 (en) * | 2014-06-12 | 2019-01-01 | University Of Notre Dame | Microfluidic devices, systems, and methods for imaging tissue samples |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| KR20170007181A (ko) | 2015-07-10 | 2017-01-18 | 3스캔 인크. | 조직학적 염색제의 공간 다중화 |
| WO2017024298A1 (en) * | 2015-08-06 | 2017-02-09 | Lasergen, Inc. | Antigen detection using photocleavable labels |
| ES2799705T3 (es) * | 2016-02-08 | 2020-12-21 | Lunaphore Tech Sa | Procedimientos de multiplexación cíclica de muestras y obtención de imágenes in situ |
| JP6952708B2 (ja) * | 2016-03-29 | 2021-10-20 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツングLeica Microsystems CMS GmbH | 生物試料の自己完結型スライド処理ユニット |
| JP7100670B2 (ja) * | 2017-05-26 | 2022-07-13 | ベンタナ メディカル システムズ, インコーポレイテッド | 非接触のスライド上流体混合 |
| JP2022535055A (ja) * | 2019-06-02 | 2022-08-04 | エッセンリックス コーポレーション | 画像処理及び人工知能によって改良された、生体物質の高速染色法 |
| CN114556074A (zh) * | 2019-06-14 | 2022-05-27 | 阿科亚生物科学股份有限公司 | 多重组织成像 |
| CN112113822A (zh) * | 2019-06-21 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | 生物样本染色装置、推片染色机及生物样本染色方法 |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| WO2021236625A1 (en) | 2020-05-19 | 2021-11-25 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| US20220062898A1 (en) | 2020-08-28 | 2022-03-03 | Leica Microsystems Cms Gmbh | Microfluidic Device for Image Multiplexing |
| EP4491742A3 (en) | 2020-09-18 | 2025-05-21 | 10x Genomics, Inc. | Sample handling apparatus and image registration methods |
| EP4294571B8 (en) | 2021-02-19 | 2024-07-10 | 10X Genomics, Inc. | Method of using a modular assay support device |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4180060A (en) * | 1972-08-18 | 1979-12-25 | Boehringer Mannheim Gmbh | Device for staining biological materials |
| US4364522A (en) * | 1980-07-21 | 1982-12-21 | General Motors Corporation | High intensity air blast fuel nozzle |
| US5595707A (en) * | 1990-03-02 | 1997-01-21 | Ventana Medical Systems, Inc. | Automated biological reaction apparatus |
| JPH0545549U (ja) * | 1991-03-22 | 1993-06-18 | 澄晴 野地 | 生物組織の処理装置 |
| US5355439A (en) * | 1991-08-05 | 1994-10-11 | Bio Tek Instruments | Method and apparatus for automated tissue assay |
| US5395588A (en) * | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
| JPH0727682A (ja) * | 1993-07-09 | 1995-01-31 | Omron Corp | 細胞診標本作製方法 |
| US5732150A (en) * | 1995-09-19 | 1998-03-24 | Ihc Health Services, Inc. | Method and system for multiple wavelength microscopy image analysis |
| JPH09271787A (ja) * | 1996-04-03 | 1997-10-21 | Mitsubishi Gas Chem Co Inc | 染料含有水溶液の脱色方法 |
| DE19709348C2 (de) * | 1996-05-29 | 1999-07-01 | Schubert Walter Dr Md | Automatisches Multi-Epitop-Ligand-Kartierungsverfahren |
| DE19803349A1 (de) * | 1997-02-26 | 1998-08-27 | Linn Geraetebau Gmbh | Verfahren und Vorrichtung zum Belüften und/oder Anreichern von Wasser, insbesondere in Gewässern mittels gasförmiger Medien, insbesondere mittels Sauerstoff |
| US6096271A (en) * | 1998-02-27 | 2000-08-01 | Cytologix Corporation | Random access slide stainer with liquid waste segregation |
| WO2001007890A2 (en) * | 1999-07-21 | 2001-02-01 | Dako A/S | A method of controlling the temperature of a specimen in or on a solid support member |
| DE10014685B4 (de) * | 2000-03-24 | 2004-07-01 | Schubert, Walter, Dr. | Verfahren zur Identifizierung von zellspezifischen Zielstrukturen |
| DE10041227B4 (de) * | 2000-08-22 | 2011-12-29 | Leica Biosystems Nussloch Gmbh | Vorrichtung zur Behandlung von Objekten |
| AU2002213043A1 (en) * | 2000-10-06 | 2002-04-15 | Protasis Corporation | Fluid separation conduit cartridge |
| WO2002079771A1 (en) * | 2001-03-30 | 2002-10-10 | The Samuel Roberts Noble Foundation, Inc. | Silver destaining method |
| US6627461B2 (en) * | 2001-04-18 | 2003-09-30 | Signature Bioscience, Inc. | Method and apparatus for detection of molecular events using temperature control of detection environment |
| JP3899845B2 (ja) * | 2001-05-23 | 2007-03-28 | 日本光電工業株式会社 | 染色標本の脱色方法および装置 |
| US20050019223A1 (en) * | 2001-08-10 | 2005-01-27 | Platt Albert Edward | Liquid delivery apparatus and method |
| JP2003114173A (ja) * | 2001-10-03 | 2003-04-18 | Canon Inc | 液体搬送装置およびその製造方法 |
| US6858432B2 (en) * | 2002-08-29 | 2005-02-22 | Wescor, Inc. | Method and staining reagent for staining hematology sample in an automated staining apparatus |
| US7648678B2 (en) * | 2002-12-20 | 2010-01-19 | Dako Denmark A/S | Method and system for pretreatment of tissue slides |
| AU2003900780A0 (en) * | 2003-02-21 | 2003-03-13 | Vision Biosystems Limited | Analysis system and procedure |
| EP1684808B1 (en) * | 2003-11-07 | 2013-01-09 | Newport Instruments | A reagent system and method for modifying the luminescence of lanthanide(iii) macrocyclic complexes |
| US7657070B2 (en) * | 2006-01-20 | 2010-02-02 | Sakura Finetek U.S.A., Inc. | Automated system of processing biological specimens and method |
| JP2007232560A (ja) * | 2006-03-01 | 2007-09-13 | Kobe Univ | 簡便迅速グラム染色方法と装置 |
| WO2007134189A2 (en) * | 2006-05-10 | 2007-11-22 | Board Of Regents, The University Of Texas System | Detecting tumor biomarker in oral cancer |
-
2008
- 2008-04-02 US US12/061,044 patent/US20090253163A1/en not_active Abandoned
-
2009
- 2009-04-01 WO PCT/US2009/039052 patent/WO2009124099A1/en not_active Ceased
- 2009-04-01 EP EP09726485A patent/EP2260283A1/en not_active Withdrawn
- 2009-04-01 JP JP2011503120A patent/JP5518834B2/ja not_active Expired - Fee Related
- 2009-04-01 CN CN200980112550XA patent/CN101983327A/zh active Pending
-
2012
- 2012-02-03 US US13/365,525 patent/US20120135449A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN101983327A (zh) | 2011-03-02 |
| JP2011518320A (ja) | 2011-06-23 |
| WO2009124099A1 (en) | 2009-10-08 |
| US20090253163A1 (en) | 2009-10-08 |
| US20120135449A1 (en) | 2012-05-31 |
| EP2260283A1 (en) | 2010-12-15 |
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