JP5497986B2 - 突然変異体ａｏｘ１プロモーター - Google Patents

突然変異体ａｏｘ１プロモーター Download PDF

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Publication number
JP5497986B2
JP5497986B2 JP2007556461A JP2007556461A JP5497986B2 JP 5497986 B2 JP5497986 B2 JP 5497986B2 JP 2007556461 A JP2007556461 A JP 2007556461A JP 2007556461 A JP2007556461 A JP 2007556461A JP 5497986 B2 JP5497986 B2 JP 5497986B2
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promoter
cell
nucleic acid
aox1
pichia pastoris
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Japanese (ja)
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JP2008531000A (ja
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フランツ・ハルトナー
アントン・グリーダー
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VTU Holding GmbH
Technische Universitaet Graz
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VTU Holding GmbH
Technische Universitaet Graz
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2007556461A 2005-02-23 2006-02-23 突然変異体ａｏｘ１プロモーター Expired - Lifetime JP5497986B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA304/2005 2005-02-23
AT0030405A AT501955B1 (de) 2005-02-23 2005-02-23 Mutierte aox1-promotoren
PCT/AT2006/000079 WO2006089329A2 (en) 2005-02-23 2006-02-23 Mutant aox 1 promoters

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011245688A Division JP5552107B2 (ja) 2005-02-23 2011-11-09 突然変異体ａｏｘ１プロモーター

Publications (2)

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JP2008531000A JP2008531000A (ja) 2008-08-14
JP5497986B2 true JP5497986B2 (ja) 2014-05-21

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JP2007556461A Expired - Lifetime JP5497986B2 (ja) 2005-02-23 2006-02-23 突然変異体ａｏｘ１プロモーター
JP2011245688A Expired - Lifetime JP5552107B2 (ja) 2005-02-23 2011-11-09 突然変異体ａｏｘ１プロモーター

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JP2011245688A Expired - Lifetime JP5552107B2 (ja) 2005-02-23 2011-11-09 突然変異体ａｏｘ１プロモーター

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US (2) US9012175B2 (https=)
EP (2) EP2199389B1 (https=)
JP (2) JP5497986B2 (https=)
KR (2) KR101352399B1 (https=)
CN (2) CN101128581B (https=)
AT (3) AT501955B1 (https=)
AU (2) AU2006216094B2 (https=)
BR (1) BRPI0606179B1 (https=)
CA (2) CA2598514C (https=)
DE (2) DE602006018113D1 (https=)
DK (2) DK1851312T5 (https=)
ES (2) ES2356127T3 (https=)
HR (2) HRP20110084T2 (https=)
IN (1) IN2012DN06694A (https=)
NZ (2) NZ585821A (https=)
PL (2) PL2199389T3 (https=)
PT (1) PT1851312E (https=)
SI (2) SI1851312T1 (https=)
UA (2) UA98604C2 (https=)
WO (1) WO2006089329A2 (https=)

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BRPI0906241B8 (pt) * 2008-03-27 2019-02-19 Alliance Sustainable Energy molécula de ácido nucleico, gene quimérico, vetor e método de transformação de uma célula bacteriana
WO2010004042A2 (en) * 2008-07-11 2010-01-14 Novozymes A/S Pichia pastoris das promoter variants
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
US20140011236A1 (en) * 2011-03-22 2014-01-09 Merch Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
GB201113419D0 (en) * 2011-08-04 2011-09-21 Fujifilm Diosynth Biotechnologies Uk Ltd Yeast vector
EP2565262A1 (en) 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
TWI560272B (en) 2011-10-07 2016-12-01 Lonza Ag Regulatable promoter
WO2014088563A1 (en) * 2012-12-05 2014-06-12 Exxonmobil Research And Engineering Company Nannochloropsis kozak consensus sequence
DK3527667T3 (da) 2013-03-08 2021-02-22 Biogrammatics Inc Gærpromotorer til proteinekspression
ES2738181T3 (es) 2013-03-08 2020-01-20 Keck Graduate Inst Of Applied Life Sciences Promotores de levadura de Pichia pastoris
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
JP6698352B2 (ja) 2013-03-15 2020-05-27 ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ 構成的プロモーター
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
EP3294762B1 (en) 2015-05-11 2022-01-19 Impossible Foods Inc. Expression constructs and methods of genetically engineering methylotrophic yeast
DK3332005T3 (da) 2015-08-05 2022-01-03 Lonza Ag Promotorvarianter
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR102596200B1 (ko) * 2017-06-30 2023-10-30 다우 글로벌 테크놀로지스 엘엘씨 알데히드 개선을 위한 코팅 및 이의 제조 방법
EP3802832A1 (en) 2018-06-07 2021-04-14 Basf Se Promoter for yeast
EP3824083A4 (en) * 2018-09-24 2022-01-05 Orta Dogu Teknik Universitesi Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering
JP2022502018A (ja) * 2018-09-24 2022-01-11 オルタ ドグ テクニク ユニヴェルシテシ 改変されたａｏｘ１プロモータバリアント
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
CN109735547B (zh) * 2019-03-19 2022-05-31 青岛蔚蓝生物集团有限公司 一种提高毕赤酵母外源蛋白表达量的启动子及其应用
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
KR20210153106A (ko) * 2019-04-17 2021-12-16 임파서블 푸즈 인크. 단백질 생산을 위한 물질 및 방법
CN110106168B (zh) * 2019-05-27 2021-07-13 西南大学 基于转录因子间的互作关系构建双激素响应启动子的方法
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN110724720A (zh) * 2019-11-01 2020-01-24 山东仙普爱瑞科技股份有限公司 一种猪α-干扰素的发酵工艺
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
IL304910A (en) 2021-02-12 2023-10-01 Boehringer Ingelheim Rcv Gmbh Signal peptides for increased protein secretion
CA3131181C (en) * 2021-06-24 2023-03-07 BioBoost Synbio Consulting Inc. Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products
EP4726051A1 (en) 2023-06-06 2026-04-15 FUJIFILM Corporation Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast
CN121358867A (zh) * 2023-06-15 2026-01-16 丹斯塔发酵股份公司 C2碳源响应性启动子
CN117417934B (zh) * 2023-10-27 2024-09-13 江南大学 跨菌种启动子、多菌种穿梭质粒及其应用
WO2025104126A1 (en) 2023-11-14 2025-05-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2025163019A1 (en) 2024-02-01 2025-08-07 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN118599913B (zh) * 2024-08-07 2024-12-27 南昌大学 醛氧化酶Aox1基因敲除动物模型的构建方法及应用

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CA2063890C (en) * 1991-03-27 2007-03-13 Masami Miura Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
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JP3290108B2 (ja) * 1997-08-29 2002-06-10 麒麟麦酒株式会社 プロモーター活性を増大させるｄｎａ配列
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US6699691B2 (en) * 2001-04-05 2004-03-02 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
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Also Published As

Publication number Publication date
ES2358086T3 (es) 2011-05-05
KR101352399B1 (ko) 2014-02-18
ES2356127T3 (es) 2011-04-05
AT501955B1 (de) 2007-08-15
US20100196913A1 (en) 2010-08-05
AU2011200187B2 (en) 2012-01-19
DE602006018113D1 (de) 2010-12-23
PL1851312T3 (pl) 2011-05-31
HRP20110254T1 (hr) 2011-05-31
NZ560542A (en) 2010-06-25
US20080153126A1 (en) 2008-06-26
ES2356127T9 (es) 2011-05-30
CA2785161C (en) 2020-03-31
HK1113172A1 (en) 2008-09-26
AU2006216094B2 (en) 2010-10-21
DK1851312T3 (da) 2011-02-28
UA94399C2 (ru) 2011-05-10
NZ585821A (en) 2011-02-25
CN101128581B (zh) 2012-04-18
EP2199389B1 (en) 2011-01-19
US9279129B2 (en) 2016-03-08
US9012175B2 (en) 2015-04-21
PL2199389T3 (pl) 2011-06-30
KR101358663B1 (ko) 2014-02-20
HRP20110084T2 (hr) 2011-10-31
CN101935657A (zh) 2011-01-05
JP2012080886A (ja) 2012-04-26
CA2785161A1 (en) 2006-08-31
WO2006089329A8 (en) 2006-11-02
EP2199389A1 (en) 2010-06-23
JP5552107B2 (ja) 2014-07-16
JP2008531000A (ja) 2008-08-14
ATE487788T1 (de) 2010-11-15
CN101935657B (zh) 2013-02-13
SI2199389T1 (sl) 2011-05-31
EP1851312B9 (en) 2011-04-20
WO2006089329A2 (en) 2006-08-31
AT501955A1 (de) 2006-12-15
BRPI0606179B1 (pt) 2018-05-29
KR20120106979A (ko) 2012-09-27
AU2006216094A1 (en) 2006-08-31
DE602006019789D1 (de) 2011-03-03
BRPI0606179A2 (pt) 2009-06-02
KR20070107781A (ko) 2007-11-07
PT1851312E (pt) 2011-01-05
HRP20110084T1 (hr) 2011-03-31
IN2012DN06694A (https=) 2015-10-23
CA2598514A1 (en) 2006-08-31
CN101128581A (zh) 2008-02-20
DK1851312T5 (da) 2011-04-18
UA98604C2 (ru) 2012-05-25
SI1851312T1 (sl) 2011-03-31
EP1851312A2 (en) 2007-11-07
ATE496125T1 (de) 2011-02-15
AU2011200187A1 (en) 2011-02-03
CA2598514C (en) 2013-07-09
WO2006089329A3 (en) 2007-05-31
DK2199389T3 (da) 2011-04-11
EP1851312B1 (en) 2010-11-10
HK1148313A1 (en) 2011-09-02

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