JP5376478B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents

効率的な人工多能性幹細胞の樹立方法 Download PDF

Info

Publication number
JP5376478B2
JP5376478B2 JP2012523493A JP2012523493A JP5376478B2 JP 5376478 B2 JP5376478 B2 JP 5376478B2 JP 2012523493 A JP2012523493 A JP 2012523493A JP 2012523493 A JP2012523493 A JP 2012523493A JP 5376478 B2 JP5376478 B2 JP 5376478B2
Authority
JP
Japan
Prior art keywords
cells
vector
nucleic acid
pcxle
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012523493A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013501505A (ja
JP2013501505A5 (es
Inventor
圭介 沖田
誠人 中川
伸弥 山中
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University
Original Assignee
Kyoto University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University filed Critical Kyoto University
Publication of JP2013501505A publication Critical patent/JP2013501505A/ja
Publication of JP2013501505A5 publication Critical patent/JP2013501505A5/ja
Application granted granted Critical
Publication of JP5376478B2 publication Critical patent/JP5376478B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1361—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from dental pulp or dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012523493A 2009-08-07 2010-08-06 効率的な人工多能性幹細胞の樹立方法 Active JP5376478B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US23240209P 2009-08-07 2009-08-07
US61/232,402 2009-08-07
US30730610P 2010-02-23 2010-02-23
US61/307,306 2010-02-23
PCT/JP2010/063733 WO2011016588A1 (en) 2009-08-07 2010-08-06 Method of efficiently establishing induced pluripotent stem cells

Publications (3)

Publication Number Publication Date
JP2013501505A JP2013501505A (ja) 2013-01-17
JP2013501505A5 JP2013501505A5 (es) 2013-03-21
JP5376478B2 true JP5376478B2 (ja) 2013-12-25

Family

ID=43544479

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012523493A Active JP5376478B2 (ja) 2009-08-07 2010-08-06 効率的な人工多能性幹細胞の樹立方法

Country Status (10)

Country Link
US (2) US8900871B2 (es)
EP (1) EP2462225B1 (es)
JP (1) JP5376478B2 (es)
KR (1) KR101774206B1 (es)
CN (1) CN102625837B (es)
AU (1) AU2010279913B2 (es)
CA (1) CA2770412C (es)
ES (1) ES2726766T3 (es)
SG (2) SG10201608797WA (es)
WO (1) WO2011016588A1 (es)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2933478T3 (es) 2005-08-03 2023-02-09 Astellas Inst For Regenerative Medicine Métodos mejorados de reprogramación de células somáticas animales
US20110061118A1 (en) * 2008-03-17 2011-03-10 Helmholtz Zentrum Munchen Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
RU2010139426A (ru) * 2008-03-17 2012-04-27 Зе Скрипс Ресеч Инститьют (Us) Комбинация химического и генетического подходов к получению индуцированных плюрипотентных стволовых клеток
EP2307539B1 (en) * 2008-07-30 2014-11-19 Kyoto University Method of efficiently establishing induced pluripotent stem cells
EP3312269A1 (en) 2008-12-17 2018-04-25 The Scripps Research Institute Generation and maintenance of stem cells
WO2010098419A1 (en) 2009-02-27 2010-09-02 Kyoto University Novel nuclear reprogramming substance
ES2726766T3 (es) 2009-08-07 2019-10-09 Univ Kyoto Método para el desarrollo eficiente de células madre pluripotentes inducidas
JP5773393B2 (ja) 2009-09-24 2015-09-02 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
JP5808331B2 (ja) 2009-10-16 2015-11-10 ザ スクリプス リサーチ インスティテュート 多能性細胞の誘導法
JP5898086B2 (ja) 2009-11-04 2016-04-06 セルラー ダイナミクス インターナショナル， インコーポレイテッド 化学物質を用いるエピソームリプログラミング
JP5765746B2 (ja) 2010-02-16 2015-08-19 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2011119942A1 (en) * 2010-03-25 2011-09-29 Vistagen Therapeutics, Inc. Induction of ips cells using transient episomal vectors
CN102959076B (zh) 2010-03-31 2015-09-16 斯克里普斯研究所 重编程细胞
ES2685171T3 (es) 2010-06-14 2018-10-05 The Scripps Research Institute Reprogramación de células a un nuevo destino
EP2582794B2 (en) * 2010-06-15 2024-04-24 FUJIFILM Cellular Dynamics, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
US9447408B2 (en) 2010-09-14 2016-09-20 Kyoto University Method of efficiently establishing induced pluripotent stem cells
WO2012051515A2 (en) * 2010-10-14 2012-04-19 University Of Central Florida Research Foundation, Inc. Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium
US9732319B2 (en) 2010-12-22 2017-08-15 Fate Therapeutics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
PL2694642T3 (pl) 2011-04-08 2019-01-31 Institut National De La Santé Et De La Recherche Médicale (Inserm) Sposób odmładzania komórek
US9758765B2 (en) 2011-04-08 2017-09-12 Osaka University Modified laminin and use thereof
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
WO2013022022A1 (ja) * 2011-08-08 2013-02-14 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
US10391126B2 (en) 2011-11-18 2019-08-27 Board Of Regents, The University Of Texas System CAR+ T cells genetically modified to eliminate expression of T-cell receptor and/or HLA
US20130157365A1 (en) * 2011-12-20 2013-06-20 Advanced Technologies And Regenerative Medicine, Llc Induced pluripotent stem cells from human umbilical cord tissue-derived cells
US20130157368A1 (en) * 2011-12-20 2013-06-20 Advanced Technologies And Regenerative Medicine, Llc Induced pluripotent stem cells prepared from human kidney-derived cells
US10119150B2 (en) * 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
ES2725827T3 (es) * 2012-05-23 2019-09-27 Univ Kyoto Método altamente eficiente para establecer una célula madre pluripotencial artificial
CN102965393B (zh) * 2012-11-06 2015-08-26 上海交通大学 人诱导多能干细胞的制备方法及其用途
DK2951290T3 (en) 2013-02-01 2018-01-22 Us Health PROCEDURE FOR PREPARING RETINAL PIGMENTAL EPIT (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCS)
WO2014192312A1 (ja) * 2013-05-31 2014-12-04 国立大学法人 東京医科歯科大学 正常な内向きのカリウム電流特性を有するｉＰＳ細胞由来心筋モデル細胞
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
CN103571794B (zh) * 2013-07-19 2016-01-20 中国医学科学院血液病医院（血液学研究所） 一种抑制PUMA功能改善iPS细胞建立功效的方法
CN103725710B (zh) * 2013-12-25 2015-10-28 北京济福霖生物技术有限公司 一种可自我删除游离载体及其应用
CN103740756B (zh) * 2013-12-25 2015-08-05 中国农业大学 一种可调控删除的非病毒游离载体及其构建方法
CN103740757B (zh) * 2014-01-21 2016-04-20 中国科学院生物物理研究所 一种利用重编程制备猪神经干细胞的方法
CN104830906B (zh) * 2014-02-12 2018-09-04 北京维通达生物技术有限公司 一种重编程获得功能性人肝脏实质细胞的方法
EP3805369A1 (en) 2014-03-04 2021-04-14 Fate Therapeutics, Inc. Improved reprogramming methods and cell culture platforms
US10344285B2 (en) 2014-04-09 2019-07-09 Dna2.0, Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
KR20160145186A (ko) * 2014-04-24 2016-12-19 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 입양 세포 요법 생성물을 생성하기 위한 유도 만능 줄기 세포의 응용
US20170107498A1 (en) * 2014-06-05 2017-04-20 Cedars-Sinai Medical Center Novel and efficient method for reprogramming immortalized lymphoblastoid cell lines to induced pluripotent stem cells
WO2016117139A1 (ja) * 2015-01-20 2016-07-28 タカラバイオ株式会社 神経系細胞の製造方法
JP2018509890A (ja) * 2015-01-30 2018-04-12 センター ナショナル ド ラ ルシェルシュ サイエンティフィーク 人工多能性幹細胞（ｉＰＳＣ）の生産を再プログラムする方法
CN107787367B (zh) 2015-04-06 2021-10-26 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna
US11020463B2 (en) 2015-08-07 2021-06-01 The J. David Gladstone Institutes Method of determining cellular protein homeostasis
ES2903442T3 (es) 2015-09-08 2022-04-01 Fujifilm Cellular Dynamics Inc Purificación basada en MACS del epitelio pigmentario de la retina derivado de células madre
AU2016321170B2 (en) 2015-09-08 2022-09-01 Fujifilm Cellular Dynamics Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
LT3359671T (lt) 2015-10-08 2021-12-10 Dna Twopointo Inc. Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
WO2017070337A1 (en) 2015-10-20 2017-04-27 Cellular Dynamics International, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
AU2017254268B2 (en) 2016-04-22 2023-03-16 Kyoto University Method for producing dopamine-producing neural precursor cells
CN109641020B (zh) * 2016-04-29 2022-09-02 复诺健生物科技加拿大有限公司 在癌细胞中具有增强复制的hsv载体
GB201608944D0 (en) * 2016-05-20 2016-07-06 Ospedale San Raffaele And Fond Telethon Gene Tharapy
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US20180030478A1 (en) 2016-07-01 2018-02-01 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
EP3491134B1 (en) 2016-08-01 2023-10-11 University of Pittsburgh - of The Commonwealth System of Higher Education Human induced pluripotent stem cells for high efficiency genetic engineering
EP3510151A4 (en) 2016-09-09 2020-04-15 The Board of Trustees of the Leland Stanford Junior University HIGH-THROUGHPUT PRECISION GENE EDITING
CN110050061A (zh) 2016-10-05 2019-07-23 富士胶片细胞动力公司 从具有MeCP2破坏的诱导多能干细胞生成成熟谱系
JP7212615B2 (ja) * 2016-10-05 2023-01-25 フジフィルム セルラー ダイナミクス，インコーポレイテッド 多能性幹細胞からｈｌａホモ接合免疫細胞への指向分化方法
US11458225B2 (en) 2016-11-09 2022-10-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services 3D vascularized human ocular tissue for cell therapy and drug discovery
WO2018144546A1 (en) * 2017-02-01 2018-08-09 President And Fellows Of Harvard College Methods for increasing efficiency of gene editing in cells
CN106939299B (zh) * 2017-02-04 2022-11-15 滨州医学院 microRNA重编程体细胞为神经干细胞的制备方法及应用
US11530388B2 (en) 2017-02-14 2022-12-20 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
JP7181219B2 (ja) 2017-04-18 2022-11-30 フジフィルム セルラー ダイナミクス，インコーポレイテッド 抗原特異的免疫エフェクター細胞
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
JP2021521792A (ja) 2018-04-20 2021-08-30 フジフィルム セルラー ダイナミクス，インコーポレイテッド 眼球細胞の分化方法及びその使用
WO2020106622A1 (en) 2018-11-19 2020-05-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodegradable tissue replacement implant and its use
WO2020113029A2 (en) 2018-11-28 2020-06-04 Board Of Regents, The University Of Texas System Multiplex genome editing of immune cells to enhance functionality and resistance to suppressive environment
KR20210096648A (ko) 2018-11-29 2021-08-05 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 자연 살해 세포의 생체외 확장을 위한 방법 및 이의 용도
CN109679918A (zh) * 2018-12-25 2019-04-26 合肥中科干细胞再生医学有限公司 一种便捷的人诱导性多能干细胞的制备方法
CN109666650A (zh) * 2019-01-04 2019-04-23 北京航空航天大学 一种细胞重编程方法
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法
IL294715A (en) 2020-01-23 2022-09-01 Childrens Medical Ct Corp Inducible t-cell differentiation from human pluripotent stem cells
US20230055044A1 (en) * 2020-01-24 2023-02-23 I Peace, Inc. Method for manufacturing induced pluripotent stem cells
KR20230019453A (ko) 2020-05-29 2023-02-08 후지필름 셀룰러 다이내믹스, 인코포레이티드 망막 색소 상피 세포 및 광수용체의 이중 세포 응집체 및 이의 사용 방법
CN116323677A (zh) 2020-05-29 2023-06-23 富士胶片细胞动力公司 视网膜色素上皮和光感受器双层及其用途
CN116829721A (zh) * 2020-07-20 2023-09-29 细胞研究私人有限公司 一种产生诱导多能干细胞的方法、诱导多能干细胞和使用所述诱导多能干细胞的方法
CN114149974A (zh) * 2020-09-08 2022-03-08 是光隽恒(北京)生物科技有限公司 诱导间充质干细胞成为多能干细胞的方法
IL302728A (en) 2020-11-13 2023-07-01 Catamaran Bio Inc Genetically modified natural killer cells and methods of using them
US20220162288A1 (en) 2020-11-25 2022-05-26 Catamaran Bio, Inc. Cellular therapeutics engineered with signal modulators and methods of use thereof
GB202101107D0 (en) * 2021-01-27 2021-03-10 Oxford Genetics Ltd Method of genome editing
US20220389436A1 (en) 2021-05-26 2022-12-08 FUJIFILM Cellular Dynamics, Inc. Methods to prevent rapid silencing of genes in pluripotent stem cells
CA3220433A1 (en) 2021-05-28 2022-12-01 Arvydas Maminishkis Biodegradable tissue scaffold with secondary matrix to host weakly adherent cells
JP2024520424A (ja) 2021-05-28 2024-05-24 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 黄斑、中心および末梢網膜色素上皮細胞を生成する方法
WO2023039586A1 (en) 2021-09-10 2023-03-16 Agilent Technologies, Inc. Guide rnas with chemical modification for prime editing
KR20240056604A (ko) 2021-09-13 2024-04-30 후지필름 셀룰러 다이내믹스, 인코포레이티드 수임 심장 전구세포의 제조 방법
CN113862221B (zh) * 2021-10-27 2024-04-30 上海爱萨尔生物科技有限公司 外周血单个核细胞的培养液及培养方法
US20240003871A1 (en) 2022-06-29 2024-01-04 FUJIFILM Cellular Dynamics, Inc. Ipsc-derived astrocytes and methods of use thereof
CN115044590B (zh) * 2022-06-30 2023-08-15 昆明理工大学 p53基因突变体及其表达的蛋白在制备诊断和治疗肥厚型心肌病的药物中的应用
WO2024073776A1 (en) 2022-09-30 2024-04-04 FUJIFILM Cellular Dynamics, Inc. Methods for the production of cardiac fibroblasts

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
NZ513487A (en) 1999-01-29 2003-02-28 Univ Illinois The use of a P53 inhibitor for the treatment of cancer, hyperthermia, hypoxia, a burn, trauma to the central nervous system, a seizure, acute inflammation, tissue ageing, preservation of organs for transplant and preparation of a host for bone marrow transplant
ATE367445T1 (de) 1999-05-18 2007-08-15 Dnavec Research Inc Viraler paramyxoviridae vektor mit einem defekten hüllprotein-gen
EP1859026A2 (en) 2005-01-31 2007-11-28 ES Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
CN103113463B (zh) 2005-12-13 2015-02-18 国立大学法人京都大学 核重新编程因子
EP1981970A4 (en) * 2006-01-11 2009-10-21 Technion Res & Dev Foundation TISSUE CONSTRUCTION TISSUE TREATMENT DERIVED FROM A HUMAN EMBRYONIC STEM CELL
US8440461B2 (en) 2007-03-23 2013-05-14 Wisconsin Alumni Research Foundation Reprogramming somatic cells using retroviral vectors comprising Oct-4 and Sox2 genes
WO2008144580A2 (en) * 2007-05-17 2008-11-27 Oregon Health & Science University Primate stem cells produced by somatic cell nuclear transfer
AU2008297024B2 (en) * 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
KR101532442B1 (ko) 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 효율적인 핵 초기화 방법
US20110061118A1 (en) 2008-03-17 2011-03-10 Helmholtz Zentrum Munchen Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
CN101550406B (zh) 2008-04-03 2016-02-10 北京大学 制备多潜能干细胞的方法,试剂盒及用途
KR101648019B1 (ko) 2008-06-04 2016-08-16 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 비-바이러스 접근법을 사용한 ｉＰＳ 세포의 생산 방법
JP2011524174A (ja) * 2008-06-13 2011-09-01 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 細胞のプログラミングおよび再プログラミング
CN101835890B (zh) 2008-06-27 2013-07-24 国立大学法人京都大学 有效建立诱导的多能干细胞的方法
EP2307539B1 (en) 2008-07-30 2014-11-19 Kyoto University Method of efficiently establishing induced pluripotent stem cells
JP5553178B2 (ja) 2008-07-31 2014-07-16 国立大学法人岐阜大学 効率的な人工多能性幹細胞の樹立方法
US20110003365A1 (en) 2009-05-29 2011-01-06 Kyoto University Method of preparing induced pluripotent stem cells deprived of reprogramming gene
ES2726766T3 (es) 2009-08-07 2019-10-09 Univ Kyoto Método para el desarrollo eficiente de células madre pluripotentes inducidas
CN102803476A (zh) 2009-09-14 2012-11-28 程临钊 血细胞重编程产生多潜能干细胞和多功能干细胞
JP5765746B2 (ja) 2010-02-16 2015-08-19 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2011119942A1 (en) 2010-03-25 2011-09-29 Vistagen Therapeutics, Inc. Induction of ips cells using transient episomal vectors
CA2806858C (en) 2010-08-04 2021-06-15 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
WO2013022022A1 (ja) 2011-08-08 2013-02-14 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
ES2725827T3 (es) 2012-05-23 2019-09-27 Univ Kyoto Método altamente eficiente para establecer una célula madre pluripotencial artificial

Also Published As

Publication number Publication date
EP2462225A1 (en) 2012-06-13
JP2013501505A (ja) 2013-01-17
ES2726766T3 (es) 2019-10-09
SG178197A1 (en) 2012-03-29
US8900871B2 (en) 2014-12-02
EP2462225A4 (en) 2013-12-18
KR101774206B1 (ko) 2017-09-04
US20120196360A1 (en) 2012-08-02
EP2462225B1 (en) 2019-03-06
CN102625837B (zh) 2015-01-14
AU2010279913A1 (en) 2012-03-15
AU2010279913B2 (en) 2016-04-28
CA2770412C (en) 2018-09-11
CN102625837A (zh) 2012-08-01
US9404124B2 (en) 2016-08-02
SG10201608797WA (en) 2016-12-29
CA2770412A1 (en) 2011-02-10
KR20120047968A (ko) 2012-05-14
US20150140662A1 (en) 2015-05-21
WO2011016588A1 (en) 2011-02-10

Similar Documents

Publication Publication Date Title
JP5376478B2 (ja) 効率的な人工多能性幹細胞の樹立方法
JP5340265B2 (ja) 効率的な人工多能性幹細胞の樹立方法
US20150184131A1 (en) Enhanced Efficiency of Induced Pluripotent Stem Cell Generation
JP5794588B2 (ja) 効率的な人工多能性幹細胞の樹立方法
JP5682043B2 (ja) 安全な多能性幹細胞の選択方法
JP6676260B2 (ja) 効率的な人工多能性幹細胞の樹立方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130123

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130123

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130402

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130603

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130806

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130813

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130903

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130917

R150 Certificate of patent or registration of utility model

Ref document number: 5376478

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250