JP5354938B2 - 蛍光顕微鏡装置 - Google Patents

蛍光顕微鏡装置 Download PDF

Info

Publication number
JP5354938B2
JP5354938B2 JP2008069246A JP2008069246A JP5354938B2 JP 5354938 B2 JP5354938 B2 JP 5354938B2 JP 2008069246 A JP2008069246 A JP 2008069246A JP 2008069246 A JP2008069246 A JP 2008069246A JP 5354938 B2 JP5354938 B2 JP 5354938B2
Authority
JP
Japan
Prior art keywords
optical system
fluorescence
light
sample
scanning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2008069246A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008276191A (ja
JP2008276191A5 (enExample
Inventor
伸二 本村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to US12/111,659 priority Critical patent/US7902523B2/en
Priority to EP08008298A priority patent/EP2037309A3/en
Publication of JP2008276191A publication Critical patent/JP2008276191A/ja
Publication of JP2008276191A5 publication Critical patent/JP2008276191A5/ja
Application granted granted Critical
Publication of JP5354938B2 publication Critical patent/JP5354938B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Multimedia (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2008069246A 2007-05-02 2008-03-18 蛍光顕微鏡装置 Expired - Fee Related JP5354938B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/111,659 US7902523B2 (en) 2007-05-02 2008-04-29 Fluorescence microscope apparatus
EP08008298A EP2037309A3 (en) 2007-05-02 2008-04-30 Fluorescence microscope apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92723707P 2007-05-02 2007-05-02
US60/927,237 2007-05-02

Publications (3)

Publication Number Publication Date
JP2008276191A JP2008276191A (ja) 2008-11-13
JP2008276191A5 JP2008276191A5 (enExample) 2011-03-24
JP5354938B2 true JP5354938B2 (ja) 2013-11-27

Family

ID=40054165

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008069246A Expired - Fee Related JP5354938B2 (ja) 2007-05-02 2008-03-18 蛍光顕微鏡装置

Country Status (3)

Country Link
US (1) US7902523B2 (enExample)
EP (1) EP2037309A3 (enExample)
JP (1) JP5354938B2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010085548A2 (en) * 2009-01-22 2010-07-29 Li-Cor, Inc. Single molecule proteomics with dynamic probes
JP5325620B2 (ja) * 2009-03-18 2013-10-23 オリンパス株式会社 画像解析装置、画像解析方法及びプログラム
JP5208824B2 (ja) * 2009-03-18 2013-06-12 オリンパス株式会社 画像取得装置、画像取得方法及びプログラム
JP5468805B2 (ja) * 2009-03-31 2014-04-09 オリンパス株式会社 画像解析方法および画像解析装置
JP5489541B2 (ja) * 2009-06-05 2014-05-14 オリンパス株式会社 画像解析方法および画像解析装置
DE102009037366A1 (de) * 2009-08-13 2011-02-17 Carl Zeiss Microlmaging Gmbh Mikroskop, insbesondere zur Messung von Totalreflexions-Fluoreszenz, und Betriebsverfahren für ein solches
JP5508809B2 (ja) * 2009-10-15 2014-06-04 オリンパス株式会社 画像解析方法および画像解析装置
JP5508808B2 (ja) * 2009-10-15 2014-06-04 オリンパス株式会社 画像解析方法および画像解析装置
JP5452180B2 (ja) * 2009-11-13 2014-03-26 オリンパス株式会社 顕微鏡装置
JP5555061B2 (ja) * 2010-06-09 2014-07-23 オリンパス株式会社 観察装置および観察方法
JP2012008055A (ja) * 2010-06-25 2012-01-12 Olympus Corp 画像解析方法および画像解析装置
JP5673679B2 (ja) * 2010-07-01 2015-02-18 株式会社ニコン 顕微鏡
CN103189737B (zh) * 2010-10-29 2017-05-31 奥林巴斯株式会社 图像分析方法以及图像分析装置
US9494783B2 (en) 2010-11-30 2016-11-15 Etaluma Inc. Compact, high-resolution fluorescence and brightfield microscope and methods of use
DE102011000090B4 (de) 2011-01-11 2016-03-24 Leica Microsystems Cms Gmbh Verfahren und Einrichtung zur rastermikroskopischen Abbildung eines Objektes
US9110282B2 (en) * 2011-03-30 2015-08-18 The Regents Of The University Of California Nanometer-scale optical imaging by the modulation tracking (MT) method
WO2013063316A1 (en) 2011-10-25 2013-05-02 Daylight Solutions, Inc. Infrared imaging microscope
JP5856814B2 (ja) * 2011-11-10 2016-02-10 オリンパス株式会社 画像解析方法および画像解析装置
JP5926966B2 (ja) * 2012-01-30 2016-05-25 オリンパス株式会社 蛍光観察装置
JP2013195127A (ja) * 2012-03-16 2013-09-30 Olympus Corp 生物試料の画像解析装置、画像解析方法、およびプログラム
JP6525161B2 (ja) 2013-04-12 2019-06-05 デイライト ソリューションズ、インコーポレイテッド 赤外光用屈折対物レンズ・アセンブリ
JP5784790B2 (ja) * 2014-04-28 2015-09-24 オリンパス株式会社 蛍光観察装置
JP6363890B2 (ja) 2014-07-04 2018-07-25 オリンパス株式会社 走査型顕微鏡装置および超解像画像の生成方法
TWI507674B (zh) * 2014-07-09 2015-11-11 Univ Nat Taiwan 以螢光物質檢測器官代謝功能之系統及方法
EP3418710B1 (en) * 2016-02-19 2022-08-24 Nikon Corporation Sample preparation method and sample preparation device
DE102016103382B3 (de) * 2016-02-25 2017-08-10 Abberior Instruments Gmbh Verfahren, Vorrichtung und Laser-Scanning-Mikroskop zum Erzeugen von gerasterten Bildern
WO2020139835A1 (en) * 2018-12-26 2020-07-02 The Regents Of The University Of California Systems and methods for two-dimensional fluorescence wave propagation onto surfaces using deep learning
EP4057047A1 (en) * 2021-03-09 2022-09-14 Leica Instruments (Singapore) Pte. Ltd. Microscope system and corresponding system, method and computer program

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5117466A (en) * 1991-04-30 1992-05-26 The United States Of America As Represented By The United States Department Of Energy Integrated fluorescence analysis system
JP3917731B2 (ja) * 1996-11-21 2007-05-23 オリンパス株式会社 レーザ走査顕微鏡
JP3861396B2 (ja) * 1997-08-01 2006-12-20 株式会社ニコン 顕微鏡用画像取得装置
JP4406108B2 (ja) * 1998-03-11 2010-01-27 オリンパス株式会社 多光子励起レーザ顕微鏡
JP2001194303A (ja) * 2000-01-13 2001-07-19 Bunshi Biophotonics Kenkyusho:Kk 蛍光分子拡散運動解析装置
US6690463B2 (en) * 2000-02-10 2004-02-10 Evotec Biosystems Ag Fluorescence intensity and lifetime distribution analysis
WO2002048693A1 (fr) * 2000-12-14 2002-06-20 Olympus Optical Co., Ltd. Analyseur fluorometrique et analyse fluorometrique
JP4136440B2 (ja) * 2002-04-26 2008-08-20 オリンパス株式会社 顕微鏡装置
JP4377811B2 (ja) * 2002-06-21 2009-12-02 オリンパス株式会社 生体分子解析装置および生体分子解析方法
US7136161B2 (en) * 2003-08-01 2006-11-14 Shimadzu Corporation Component analyzing apparatus with microchip
JP2005083980A (ja) * 2003-09-10 2005-03-31 Olympus Corp 共焦点光学顕微鏡およびそれを用いた分析方法
JP4756819B2 (ja) * 2003-10-21 2011-08-24 オリンパス株式会社 走査型顕微鏡システム
JP4468684B2 (ja) * 2003-12-05 2010-05-26 オリンパス株式会社 走査型共焦点顕微鏡装置
JP4224640B2 (ja) * 2004-03-29 2009-02-18 オリンパス株式会社 蛍光分光分析装置
DE102004032952A1 (de) * 2004-07-07 2006-01-26 Leica Microsystems Cms Gmbh Rastermikroskop und Verfahren zur Untersuchung von biologischen Proben mit einem Rastermikroskop
DE102004034963A1 (de) * 2004-07-16 2006-02-16 Carl Zeiss Jena Gmbh Verfahren zur bildlichen Erfassung von Objekten mittels eines Lichtrastermikroskopes
US7330255B2 (en) * 2004-12-09 2008-02-12 University Of Chicago Total internal reflection fluorescence apparatus
JP5132052B2 (ja) * 2005-11-30 2013-01-30 オリンパス株式会社 走査型レーザ顕微鏡の条件設定装置及び走査型レーザ顕微鏡システム

Also Published As

Publication number Publication date
EP2037309A2 (en) 2009-03-18
JP2008276191A (ja) 2008-11-13
US20080290293A1 (en) 2008-11-27
EP2037309A3 (en) 2010-01-13
US7902523B2 (en) 2011-03-08

Similar Documents

Publication Publication Date Title
JP5354938B2 (ja) 蛍光顕微鏡装置
JP5489469B2 (ja) 蛍光信号解析装置および蛍光信号解析方法
JP4377811B2 (ja) 生体分子解析装置および生体分子解析方法
JP2008276191A5 (enExample)
JP6283104B2 (ja) 光学分析装置
US20050280818A1 (en) Confocal observation system
JP2009264752A (ja) 三次元画像取得装置
JP4468507B2 (ja) 走査型レーザ顕微鏡
JP4020638B2 (ja) 走査型レーザ顕微鏡システム
JP3861000B2 (ja) 走査型レーザー顕微鏡
US9977230B2 (en) Microscope system
EP2828700A1 (en) Multi-color confocal microscope and imaging methods
JP5963487B2 (ja) レーザ顕微鏡および観察方法
JP5208824B2 (ja) 画像取得装置、画像取得方法及びプログラム
CN109884052B (zh) 基于ccd探测的减法式谐波显微成像方法
JP2004069428A (ja) 原子及び分子間力顕微鏡
EP1857538B1 (en) Microscope apparatus and cell observation method
JP2004354345A (ja) 生体分子解析装置
JP2003029151A (ja) 共焦点レーザ走査型顕微鏡装置および制御プログラム
JP7480446B2 (ja) 顕微鏡システム及び光学モジュール
JP4628845B2 (ja) 標本試料の自動追尾機能を備えた顕微鏡観察装置
JP2865298B2 (ja) 光ヘテロダイン螢光顕微鏡
JP2003248174A (ja) レーザ走査型共焦点顕微鏡装置、画像記録方法、及び記録媒体
JP2017003865A (ja) 顕微鏡および顕微鏡画像取得方法
JP2007024801A (ja) 顕微鏡制御装置及びプログラム

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110203

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110203

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20120509

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120605

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130312

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130510

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130820

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130827

R151 Written notification of patent or utility model registration

Ref document number: 5354938

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R151

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees