JP5038094B2 - レーザー走査型顕微鏡 - Google Patents
レーザー走査型顕微鏡 Download PDFInfo
- Publication number
- JP5038094B2 JP5038094B2 JP2007282653A JP2007282653A JP5038094B2 JP 5038094 B2 JP5038094 B2 JP 5038094B2 JP 2007282653 A JP2007282653 A JP 2007282653A JP 2007282653 A JP2007282653 A JP 2007282653A JP 5038094 B2 JP5038094 B2 JP 5038094B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- infrared light
- infrared
- condenser lens
- scanning microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000005540 biological transmission Effects 0.000 claims description 49
- 230000003287 optical effect Effects 0.000 claims description 26
- 238000003384 imaging method Methods 0.000 claims description 12
- 230000005284 excitation Effects 0.000 description 43
- 239000000523 sample Substances 0.000 description 23
- 238000005286 illumination Methods 0.000 description 17
- 238000001917 fluorescence detection Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 4
- 210000001747 pupil Anatomy 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007282653A JP5038094B2 (ja) | 2007-10-31 | 2007-10-31 | レーザー走査型顕微鏡 |
| US12/250,680 US7915575B2 (en) | 2007-10-31 | 2008-10-14 | Laser scanning microscope having an IR partial transmission filter for realizing oblique illumination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007282653A JP5038094B2 (ja) | 2007-10-31 | 2007-10-31 | レーザー走査型顕微鏡 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009109788A JP2009109788A (ja) | 2009-05-21 |
| JP2009109788A5 JP2009109788A5 (enExample) | 2010-12-16 |
| JP5038094B2 true JP5038094B2 (ja) | 2012-10-03 |
Family
ID=40581626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007282653A Expired - Fee Related JP5038094B2 (ja) | 2007-10-31 | 2007-10-31 | レーザー走査型顕微鏡 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US7915575B2 (enExample) |
| JP (1) | JP5038094B2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5363199B2 (ja) * | 2009-06-04 | 2013-12-11 | 日本分光株式会社 | 顕微全反射測定装置 |
| KR20130095636A (ko) * | 2010-04-09 | 2013-08-28 | 노스이스턴 유니버시티 | 튜너블 레이저-기반 적외선 이미징 시스템 및 그것의 이용 방법 |
| JP5653944B2 (ja) * | 2011-04-12 | 2015-01-14 | パナソニック株式会社 | 有機el素子の製造方法及びレーザー焦点位置設定方法 |
| WO2013063316A1 (en) | 2011-10-25 | 2013-05-02 | Daylight Solutions, Inc. | Infrared imaging microscope |
| CN103676128A (zh) * | 2012-09-17 | 2014-03-26 | 昆山尚达智机械有限公司 | 一种新型的光学比较显微仪器 |
| JP6525161B2 (ja) | 2013-04-12 | 2019-06-05 | デイライト ソリューションズ、インコーポレイテッド | 赤外光用屈折対物レンズ・アセンブリ |
| WO2016056148A1 (ja) * | 2014-10-08 | 2016-04-14 | オリンパス株式会社 | 結像光学系、照明装置および観察装置 |
| US11506877B2 (en) | 2016-11-10 | 2022-11-22 | The Trustees Of Columbia University In The City Of New York | Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees |
| US20190101489A1 (en) * | 2017-09-29 | 2019-04-04 | Michael John Darwin | Method and Apparatus for Simultaneously Measuring 3Dimensional Structures and Spectral Content of Said Structures |
| CN108387562B (zh) * | 2018-02-02 | 2020-09-15 | 中国科学院上海光学精密机械研究所 | 共聚焦显微系统中针孔轴向位置的调节方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH036511A (ja) * | 1989-06-05 | 1991-01-14 | Nikon Corp | 赤外線用可変濃度フィルタ |
| DE69418248T2 (de) | 1993-06-03 | 1999-10-14 | Hamamatsu Photonics Kk | Optisches Laser-Abtastsystem mit Axikon |
| JP2931268B2 (ja) | 1993-06-03 | 1999-08-09 | 浜松ホトニクス株式会社 | レーザスキャン光学装置 |
| JPH1020198A (ja) * | 1996-07-02 | 1998-01-23 | Olympus Optical Co Ltd | 近赤外線顕微鏡及びこれを用いた顕微鏡観察システム |
| CN1145820C (zh) | 1997-08-29 | 2004-04-14 | 奥林巴斯光学工业株式会社 | 显微镜透过明视野照明装置 |
| JP4503716B2 (ja) * | 1997-08-29 | 2010-07-14 | オリンパス株式会社 | 顕微鏡透過照明装置 |
| GB2349033B (en) * | 1998-01-27 | 2002-06-26 | Wisconsin Alumni Res Found | Signal enhancement for fluorescence microscopy |
| DE19858206C2 (de) * | 1998-12-17 | 2001-10-11 | Leica Microsystems | Verfahren zur Anpassung von Anregungsintensitäten bei einem Multiband-Fluoreszenz-Mikroskop und Multiband-Fluoreszenz-Mikroskop zur Durchführung des Verfahrens |
| DE10003570A1 (de) * | 2000-01-27 | 2001-08-02 | Leica Microsystems | Mikroskop-Aufbau |
| JP2002031758A (ja) * | 2000-07-17 | 2002-01-31 | Olympus Optical Co Ltd | 顕微鏡 |
| JP2003042845A (ja) * | 2001-07-30 | 2003-02-13 | Sony Corp | 光学装置 |
| JP2003264842A (ja) * | 2002-03-08 | 2003-09-19 | Hitachi Kokusai Electric Inc | フィルタ及び調光板及び固体撮像素子 |
| JP2004302421A (ja) * | 2003-03-17 | 2004-10-28 | Nikon Corp | 全反射顕微鏡 |
| HU227859B1 (en) * | 2005-01-27 | 2012-05-02 | E Szilveszter Vizi | Real-time 3d nonlinear microscope measuring system and its application |
| JP4512698B2 (ja) * | 2005-08-30 | 2010-07-28 | ナノフォトン株式会社 | レーザ顕微鏡 |
| US7542137B2 (en) * | 2006-07-24 | 2009-06-02 | University Of Ottawa | Pathogen detection using coherent anti-stokes Raman scattering microscopy |
| KR100829439B1 (ko) * | 2007-06-08 | 2008-05-15 | 한국표준과학연구원 | 적외선 사광파 혼합 편광 이미징 장치 |
-
2007
- 2007-10-31 JP JP2007282653A patent/JP5038094B2/ja not_active Expired - Fee Related
-
2008
- 2008-10-14 US US12/250,680 patent/US7915575B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7915575B2 (en) | 2011-03-29 |
| US20090108187A1 (en) | 2009-04-30 |
| JP2009109788A (ja) | 2009-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5038094B2 (ja) | レーザー走査型顕微鏡 | |
| JP5307353B2 (ja) | 多光子励起レーザ走査型顕微鏡および多光子励起蛍光画像取得方法 | |
| JP4315794B2 (ja) | 共焦点顕微鏡 | |
| JP3816632B2 (ja) | 走査型顕微鏡 | |
| JP5636280B2 (ja) | 光操作用の光学装置 | |
| JP5485289B2 (ja) | 分解能増進顕微鏡法 | |
| JP5162781B2 (ja) | 顕微鏡組立物 | |
| JP2017167535A (ja) | ライトフィールド顕微鏡および照明方法 | |
| JP4820759B2 (ja) | 走査型顕微鏡 | |
| KR101603726B1 (ko) | 멀티모달 현미경 | |
| JP2018146602A (ja) | 観察装置 | |
| JP5311195B2 (ja) | 顕微鏡装置 | |
| JP2007506955A (ja) | エバネッセント波照明を備えた走査顕微鏡 | |
| JP6178656B2 (ja) | 補償光学素子の設定方法及び顕微鏡 | |
| WO2014199713A1 (ja) | 共焦点レーザ走査型顕微鏡 | |
| JP6253395B2 (ja) | 画像生成システム | |
| US20070109634A1 (en) | Microscope apparatus | |
| JP5311196B2 (ja) | 顕微鏡装置 | |
| JP7006010B2 (ja) | 顕微鏡 | |
| JP3917705B2 (ja) | 走査型光学顕微鏡 | |
| WO2017158697A1 (ja) | 画像取得方法および画像取得装置 | |
| JP2018194634A (ja) | ライトフィールド顕微鏡 | |
| WO2016013518A1 (ja) | 結像光学系、照明装置、観察装置および波面回復素子 | |
| JP3539436B2 (ja) | 走査型レーザ顕微鏡装置 | |
| JP2006003747A (ja) | 光走査型観察装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20101028 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20101028 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20120404 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120410 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120530 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20120619 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20120705 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150713 Year of fee payment: 3 |
|
| R151 | Written notification of patent or utility model registration |
Ref document number: 5038094 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R151 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150713 Year of fee payment: 3 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |