JP5014980B2 - 核酸精製のための固相支持体として修飾された表面 - Google Patents

核酸精製のための固相支持体として修飾された表面 Download PDF

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JP5014980B2
JP5014980B2 JP2007504164A JP2007504164A JP5014980B2 JP 5014980 B2 JP5014980 B2 JP 5014980B2 JP 2007504164 A JP2007504164 A JP 2007504164A JP 2007504164 A JP2007504164 A JP 2007504164A JP 5014980 B2 JP5014980 B2 JP 5014980B2
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rna
dextran
dna
polynucleotide
solution
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JP2007529229A5 (https=
JP2007529229A (ja
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ゲーリー ジェイ. レーサム
シンワン ファン
リチャード シー. コンラッド
ジョン エー. ケンペイネン
ロバート エー. セッタークイスト
ブリッタン エル. パスロスケ
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アプライド バイオシステムズ リミテッド ライアビリティー カンパニー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)
JP2007504164A 2004-03-18 2005-03-18 核酸精製のための固相支持体として修飾された表面 Expired - Lifetime JP5014980B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US55427804P 2004-03-18 2004-03-18
US60/554,278 2004-03-18
US10/955,974 2004-09-30
US10/955,974 US8426126B2 (en) 2004-03-18 2004-09-30 Modified surfaces as solid supports for nucleic acid purification
PCT/US2005/009189 WO2005089929A2 (en) 2004-03-18 2005-03-18 Modified surfaces as solid supports for nucleic acid purification

Publications (3)

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JP2007529229A JP2007529229A (ja) 2007-10-25
JP2007529229A5 JP2007529229A5 (https=) 2012-06-14
JP5014980B2 true JP5014980B2 (ja) 2012-08-29

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US (2) US8426126B2 (https=)
EP (2) EP1735466B1 (https=)
JP (1) JP5014980B2 (https=)
WO (1) WO2005089929A2 (https=)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
CA2592993C (en) * 2004-12-13 2013-06-18 National University Corporation Okayama University Method for detecting methylation in genes and method for examining neoplasm through detecting methylation in genes
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
US20080113357A1 (en) * 2006-06-29 2008-05-15 Millipore Corporation Filter device for the isolation of a nucleic acid
DE102006031764B4 (de) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren
WO2008058018A2 (en) 2006-11-02 2008-05-15 Mayo Foundation For Medical Education And Research Predicting cancer outcome
WO2008071384A1 (en) * 2006-12-13 2008-06-19 Roche Diagnostics Gmbh Use of tde for the isolation of nucleic acids
WO2009070465A1 (en) * 2007-11-29 2009-06-04 New England Biolabs, Inc. Selective purification of small rnas from mixtures
EP2071034A1 (en) 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
EP2250285A2 (en) * 2008-02-15 2010-11-17 Life Technologies Corporation Methods and kits for extraction of dna
CN102203251A (zh) * 2008-05-23 2011-09-28 生命科技公司 用于dna提取的方法和试剂盒
AU2009253675A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
FI20085934A0 (fi) * 2008-10-03 2008-10-03 Wallac Oy Menetelmä ja laitteisto näytteiden eluoinnin havaitsemiseksi
WO2010056337A2 (en) 2008-11-12 2010-05-20 Caris Mpi, Inc. Methods and systems of using exosomes for determining phenotypes
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US8133683B2 (en) * 2009-01-23 2012-03-13 Taipei Medical University Methods for detecting biomolecules in a sample
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
EP3360978A3 (en) 2009-05-07 2018-09-26 Veracyte, Inc. Methods for diagnosis of thyroid conditions
US8735060B2 (en) * 2009-07-29 2014-05-27 Stratec Molecular Ag Method for isolating high-purity RNA by means of paramagnetic microparticles and nanoparticles
EP2478088B1 (en) 2009-09-16 2016-08-31 Life Technologies Corporation Lysis buffers for extracting nucleic acids
CA2782284A1 (en) 2009-11-30 2011-06-03 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Methods and systems for isolating, storing, and analyzing vesicles
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
US20140148348A1 (en) 2010-01-13 2014-05-29 Christine Kuslich Dectection of gastrointestinal disorders
EP2542696B1 (en) 2010-03-01 2016-09-28 Caris Life Sciences Switzerland Holdings GmbH Biomarkers for theranostics
JP2013526852A (ja) 2010-04-06 2013-06-27 カリス ライフ サイエンシズ ルクセンブルク ホールディングス 疾患に対する循環バイオマーカー
US20130267443A1 (en) 2010-11-19 2013-10-10 The Regents Of The University Of Michigan ncRNA AND USES THEREOF
WO2012075133A1 (en) 2010-11-30 2012-06-07 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
JP5385427B2 (ja) 2011-08-04 2014-01-08 日本特殊陶業株式会社 点火プラグ、及び、点火装置
US10513737B2 (en) 2011-12-13 2019-12-24 Decipher Biosciences, Inc. Cancer diagnostics using non-coding transcripts
US9315853B2 (en) 2012-01-30 2016-04-19 Exact Sciences Corporation Modification of DNA on magnetic beads
EP2885640B1 (en) 2012-08-16 2018-07-18 Genomedx Biosciences, Inc. Prostate cancer prognostics using biomarkers
CN104620093B (zh) * 2012-08-23 2018-04-20 干细胞技术公司 用于快速可逆生物分子标记的组合物和方法
US9493766B2 (en) * 2013-02-04 2016-11-15 Corning Incorporated PCR reaction cleanup buffers
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法
EP3828284A1 (en) * 2013-03-15 2021-06-02 Abbott Molecular Inc. One-step procedure for the purification of nucleic acids
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
EP2976424B1 (en) 2013-03-18 2018-10-03 Qiagen GmbH Stabilisation of biological samples
US9719082B2 (en) 2013-10-31 2017-08-01 General Electric Company Substrates and associated methods for elution of nucleic acids
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9587263B2 (en) 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
WO2015146737A1 (ja) * 2014-03-26 2015-10-01 ユニバーサル・バイオ・リサーチ株式会社 核酸抽出方法及び試薬
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
US20170335396A1 (en) 2014-11-05 2017-11-23 Veracyte, Inc. Systems and methods of diagnosing idiopathic pulmonary fibrosis on transbronchial biopsies using machine learning and high dimensional transcriptional data
KR101728096B1 (ko) * 2015-03-12 2017-04-20 재단법인 아산사회복지재단 Ffpe 조직에서 핵산의 분리 방법
CN107406847A (zh) * 2015-03-20 2017-11-28 东丽株式会社 核酸的回收方法
EP3294448A4 (en) * 2015-05-14 2018-12-12 Longhorn Vaccines and Diagnostics, LLC Rapid methods for the extraction of nucleic acids from biological samples
TWI691595B (zh) * 2015-09-02 2020-04-21 創想生物科技有限公司 選擇性分離核酸之方法及套組
WO2017042819A1 (en) 2015-09-11 2017-03-16 Molecular Detection Israel Ltd. Methods for isolating microbial cells from a blood sample
GB2557818A (en) * 2015-09-25 2018-06-27 Veracyte Inc Methods and compositions that utilize transciptome sequencing data in machine learning-based classification
WO2017153974A1 (en) * 2016-03-07 2017-09-14 Caladrius Biosciences, Inc. A closed system for labelling and selecting live cells
EP3431598B1 (en) * 2016-03-17 2024-12-11 Toray Industries, Inc. Method for collecting nucleic acid
US10287625B2 (en) * 2016-03-18 2019-05-14 Norgen Biotek Corp. Methods and kits for separating nucleic acids by size
WO2018008032A1 (en) * 2016-07-07 2018-01-11 Technion Research & Development Foundation Ltd. Methods for isolation and quantification of short nucleic acid molecules
EP3504348B1 (en) 2016-08-24 2022-12-14 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
CA3035881A1 (en) * 2016-09-14 2018-03-22 Toray Industries, Inc. Method of collecting cell-free dna
WO2018132916A1 (en) 2017-01-20 2018-07-26 Genomedx Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
CA3055925A1 (en) 2017-03-09 2018-09-13 Decipher Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
AU2018251770B2 (en) * 2017-04-10 2024-03-14 Dermtech, Llc Non-invasive skin-based detection methods
EP3399034B1 (en) * 2017-05-05 2022-12-28 Siemens Healthcare Diagnostics Inc. Device for extracting nucleic acids from biological sample materials with solvent-free reagents
AU2018266733A1 (en) 2017-05-12 2020-01-16 Veracyte, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor aggressiveness
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
CN111278550B (zh) * 2017-06-30 2023-04-25 赛库乐米克斯公司 使用热塑性硅石纳米材料进行尺寸选择纯化
WO2019028285A2 (en) 2017-08-04 2019-02-07 Genomedx, Inc. USE OF SPECIFIC GENE EXPRESSION OF IMMUNE CELLS FOR THE PROGNOSIS OF PROSTATE CANCER AND THE PREDICTION OF SENSITIVITY TO RADIOTHERAPY
JP7238766B2 (ja) 2017-12-27 2023-03-14 東レ株式会社 核酸の回収方法
CA3164352A1 (en) * 2019-12-13 2021-06-17 Pacific Biosciences Of California, Inc. Rapid precipitation-driven kilobase size selection of hmw dna
US20230313281A1 (en) * 2020-10-09 2023-10-05 Takara Bio Usa, Inc. Methods and Compositions For Preparing Nucleic Acids For Genetic Analysis
WO2022079236A1 (en) * 2020-10-16 2022-04-21 Turbobeads Gmbh Methods and kits for nucleic acid purification
US20240002916A1 (en) * 2020-11-23 2024-01-04 Nuclein, Llc Direct lysis apparatus and methods for molecular diagnostics
BE1030468B1 (fr) * 2022-04-20 2023-11-21 Quantoom Biosciences S A Système et méthode pour la production d’arn
EP4508440A1 (en) 2022-04-15 2025-02-19 Quantoom Biosciences S.A. System and method for the production of rna
CN115058491A (zh) * 2022-06-30 2022-09-16 南京瑞贝西生物科技有限公司 一种基于磁珠法的引物快速脱盐纯化的方法

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452773A (en) * 1982-04-05 1984-06-05 Canadian Patents And Development Limited Magnetic iron-dextran microspheres
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
US5646263A (en) * 1994-09-19 1997-07-08 Promega Corporation High efficiency method for isolating target substances using a multisample separation device
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5753477A (en) * 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US6231982B1 (en) * 1997-12-10 2001-05-15 Dade Behring Inc. Particle reagents having reduced matrix effects and containing an aldehyde-reactive functional group
WO1999058664A1 (en) 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
DE60044977D1 (de) * 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
FR2804117B1 (fr) * 2000-01-21 2004-08-20 Bio Merieux Procede d'isolement de proteines et/ou d'acides nucleiques, complexes de particules et de proteines et/ou d'acides nucleiques, reactif et applications
JP4519247B2 (ja) 2000-03-09 2010-08-04 シスメックス株式会社 核酸の抽出精製用試薬
DE10013995A1 (de) 2000-03-22 2001-09-27 Chemagen Biopolymer Technologi Magnetische, silanisierte Trägermaterialien auf Basis von Polyvinylalkohol
US7169618B2 (en) * 2000-06-28 2007-01-30 Skold Technology Magnetic particles and methods of producing coated magnetic particles
US20020106686A1 (en) 2001-01-09 2002-08-08 Mckernan Kevin J. Methods and reagents for the isolation of nucleic acids
JP4399164B2 (ja) 2001-02-16 2010-01-13 プロメガ・コーポレーション 核酸の磁気単離および精製の方法
AU2002320058A1 (en) 2001-06-06 2002-12-16 The General Hspital Corporation Magnetic-nanoparticle conjugates and methods of use
US6902657B2 (en) 2001-06-15 2005-06-07 Amersham Biosciences Corp. Electrophoresis separation media and methods
AU2002322458A1 (en) * 2001-07-13 2003-01-29 Nanosphere, Inc. Method for immobilizing molecules onto surfaces
WO2003019188A1 (en) * 2001-08-31 2003-03-06 Imego Ab Methdo and arrangement for analyzing substances
EP1442045B1 (en) 2001-11-06 2009-01-07 Cortex Biochem Inc. Isolation and purification of nucleic acids
JP2003339379A (ja) 2002-05-24 2003-12-02 Tosoh Corp 核酸分離方法
CA2515075C (en) * 2003-02-05 2012-10-02 Iquum, Inc. Sample processing
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification

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Publication number Publication date
US20050208510A1 (en) 2005-09-22
EP2292789A1 (en) 2011-03-09
EP1735466B1 (en) 2012-12-05
WO2005089929A3 (en) 2005-11-24
US9464315B2 (en) 2016-10-11
US8426126B2 (en) 2013-04-23
US20130302810A1 (en) 2013-11-14
WO2005089929A2 (en) 2005-09-29
EP1735466A2 (en) 2006-12-27
JP2007529229A (ja) 2007-10-25

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