JP4866248B2 - 核酸増幅性能改良のための新規ヌクレオチド混合物 - Google Patents
核酸増幅性能改良のための新規ヌクレオチド混合物 Download PDFInfo
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| US60/538,815 | 2004-01-23 | ||
| US60/538,814 | 2004-01-23 | ||
| US60/538,816 | 2004-01-23 | ||
| PCT/US2005/002414 WO2005070027A2 (en) | 2004-01-23 | 2005-01-21 | A novel nucleotide mixture for improved nucleic acid amplification performance |
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| JP2007518424A JP2007518424A (ja) | 2007-07-12 |
| JP2007518424A6 JP2007518424A6 (ja) | 2007-10-11 |
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| JP2006551422A Expired - Fee Related JP4866248B2 (ja) | 2004-01-23 | 2005-01-21 | 核酸増幅性能改良のための新規ヌクレオチド混合物 |
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| US20080299568A1 (en) * | 2007-04-27 | 2008-12-04 | Scott Johnson | Materials and methods for detection of hepatitis c virus |
| JP2009284896A (ja) * | 2007-07-26 | 2009-12-10 | Fujifilm Corp | 核酸増幅方法 |
| US20090162856A1 (en) * | 2007-11-06 | 2009-06-25 | Hayato Miyoshi | Rna detection method |
| AU2009335156A1 (en) * | 2008-12-31 | 2010-07-08 | Abbott Laboratories | Primers and probes for detecting Hepatitis C virus |
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| JP2001503764A (ja) * | 1996-11-14 | 2001-03-21 | ロシュ ダイアグノスティックス ゲーエムベーハー | 安定化されたヌクレオシド三リン酸水溶液 |
Family Cites Families (37)
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| JPH09107970A (ja) * | 1995-10-17 | 1997-04-28 | Soyaku Gijutsu Kenkyusho:Kk | C型肝炎ウイルス由来核酸、該核酸を用いたウイルスの検出方法 |
| DE3164463D1 (en) * | 1980-11-12 | 1984-08-02 | Mitsubishi Chem Ind | Glutamine derivatives usable for curing immune diseases, methods for their preparation and compositions comprising said derivatives |
| US5087565A (en) * | 1984-07-02 | 1992-02-11 | Du Pont Merck Pharmaceutical Company | Synthetic process for preparation of 32 P-labeled nucleotides |
| EP0222889B1 (en) * | 1985-05-15 | 1993-10-27 | Amoco Corporation | Cytidine analogs |
| US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5032511A (en) * | 1987-03-31 | 1991-07-16 | Mitsubishi Kasei Corporation | DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| US5714596A (en) * | 1987-11-18 | 1998-02-03 | Chiron Corporation | NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus |
| US5712088A (en) * | 1987-11-18 | 1998-01-27 | Chiron Corporation | Methods for detecting Hepatitis C virus using polynucleotides specific for same |
| US5698390A (en) * | 1987-11-18 | 1997-12-16 | Chiron Corporation | Hepatitis C immunoassays |
| US5030557A (en) | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| EP0419180B1 (en) | 1989-09-18 | 1994-08-03 | Canon Kabushiki Kaisha | Ink jet recording head and ink jet apparatus having same |
| AU653712B2 (en) * | 1990-02-16 | 1994-10-13 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| US5648232A (en) * | 1993-01-21 | 1997-07-15 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Microbiological best method and reagents |
| JP3062250B2 (ja) * | 1994-03-10 | 2000-07-10 | ジェン−プローブ・インコーポレイテッド | イオン性界面活性剤による、酵素により媒介される反応に対する阻害の抑制方法 |
| US5874565A (en) * | 1995-08-29 | 1999-02-23 | Washington University | Nucleic acids comprising a highly conserved novel 3 terminal sequence element of the hepatitis C virus |
| US6127116A (en) * | 1995-08-29 | 2000-10-03 | Washington University | Functional DNA clone for hepatitis C virus (HCV) and uses thereof |
| DE19612779A1 (de) | 1996-03-29 | 1997-10-02 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR |
| EP2264045B1 (en) * | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stable compositions for nucleic acid amplification and sequencing |
| AU7100198A (en) | 1997-04-03 | 1998-10-22 | Life Technologies, Inc. | Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr) |
| ZA989950B (en) * | 1997-11-17 | 1999-05-04 | Akzo Nobel Nv | Transcription based amplification of double stranded DNA targets |
| CN1312856A (zh) * | 1998-04-27 | 2001-09-12 | 利博齐姆医药公司 | 与丙型肝炎病毒感染相关的疾病或状态的酶性核酸治疗 |
| AU4515699A (en) * | 1998-06-26 | 2000-01-17 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
| US6638714B1 (en) * | 1999-02-03 | 2003-10-28 | Ortho-Clinical Diagnostics, Inc. | Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof |
| US6300075B1 (en) | 1999-02-03 | 2001-10-09 | Ortho-Clinical Diagnostics, Inc | Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid |
| WO2000075338A2 (en) * | 1999-06-04 | 2000-12-14 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | CLONED GENONE OF INFECTIOUS HEPATITIS C VIRUS OF GENOTYPE 2a AND USES THEREOF |
| US6271004B1 (en) * | 1999-06-25 | 2001-08-07 | Display Systems Biotech A/S | Method for improved reverse transcription at high temperatures |
| WO2001036442A1 (en) * | 1999-11-17 | 2001-05-25 | Jiuping Ji | Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay |
| AU2001254670A1 (en) * | 2000-03-02 | 2001-09-12 | Akzo Nobel N.V. | Detection of hepatitis b virus rna |
| JP2004532022A (ja) * | 2001-03-26 | 2004-10-21 | サーナ・セラピューティクス・インコーポレイテッド | B型肝炎ウイルスおよびc型肝炎ウイルスの複製のオリゴヌクレオチド媒介性阻害 |
| WO2003016572A1 (en) * | 2001-08-17 | 2003-02-27 | Eli Lilly And Company | Oligonucleotide therapeutics for treating hepatitis c virus infections |
| US6783940B2 (en) * | 2001-10-31 | 2004-08-31 | Applera Corporation | Method of reducing non-specific amplification in PCR |
| US6750009B2 (en) * | 2002-01-29 | 2004-06-15 | Apath, Llc | Multiple viral replicon culture systems |
| WO2003100014A2 (en) * | 2002-05-24 | 2003-12-04 | Smithkline Beecham Corporation | Method for quantitating negative strand rna synthesis |
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2005
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- 2005-01-21 DE DE602005011754T patent/DE602005011754D1/de not_active Expired - Lifetime
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2001503764A (ja) * | 1996-11-14 | 2001-03-21 | ロシュ ダイアグノスティックス ゲーエムベーハー | 安定化されたヌクレオシド三リン酸水溶液 |
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| US20060003343A1 (en) | 2006-01-05 |
| WO2005070027A8 (en) | 2006-07-27 |
| EP1721015A2 (en) | 2006-11-15 |
| WO2005070027A2 (en) | 2005-08-04 |
| DE602005011754D1 (de) | 2009-01-29 |
| EP2042612B1 (en) | 2010-12-15 |
| ATE417938T1 (de) | 2009-01-15 |
| WO2005071117A2 (en) | 2005-08-04 |
| DE602005023586D1 (de) | 2010-10-28 |
| JP2007518425A (ja) | 2007-07-12 |
| EP1721015B1 (en) | 2010-09-15 |
| WO2005070027A3 (en) | 2006-04-06 |
| EP1706512B1 (en) | 2008-12-17 |
| EP2042612A3 (en) | 2009-06-17 |
| JP2007518424A (ja) | 2007-07-12 |
| ATE491810T1 (de) | 2011-01-15 |
| US7670779B2 (en) | 2010-03-02 |
| DE602005025424D1 (de) | 2011-01-27 |
| WO2005071117A3 (en) | 2006-04-20 |
| EP1706512A2 (en) | 2006-10-04 |
| EP2042612A2 (en) | 2009-04-01 |
| US20080248480A1 (en) | 2008-10-09 |
| US7364854B2 (en) | 2008-04-29 |
| ATE481507T1 (de) | 2010-10-15 |
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