JP4861584B2 - イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 - Google Patents
イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 Download PDFInfo
- Publication number
- JP4861584B2 JP4861584B2 JP2001528683A JP2001528683A JP4861584B2 JP 4861584 B2 JP4861584 B2 JP 4861584B2 JP 2001528683 A JP2001528683 A JP 2001528683A JP 2001528683 A JP2001528683 A JP 2001528683A JP 4861584 B2 JP4861584 B2 JP 4861584B2
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- electrode
- site
- ion channel
- electrodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199901407 | 1999-10-01 | ||
| DKPA199901407 | 1999-10-01 | ||
| DK199901407 | 1999-10-01 | ||
| PCT/DK2000/000548 WO2001025769A2 (en) | 1999-10-01 | 2000-10-02 | A substrate and a method for determining and/or monitoring electrophysiological properties of ion channels |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003511668A JP2003511668A (ja) | 2003-03-25 |
| JP2003511668A5 JP2003511668A5 (enExample) | 2007-02-01 |
| JP4861584B2 true JP4861584B2 (ja) | 2012-01-25 |
Family
ID=8104506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001528683A Expired - Lifetime JP4861584B2 (ja) | 1999-10-01 | 2000-10-02 | イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1221046B1 (enExample) |
| JP (1) | JP4861584B2 (enExample) |
| CN (1) | CN1204404C (enExample) |
| AT (1) | ATE317545T1 (enExample) |
| AU (1) | AU780157B2 (enExample) |
| CA (1) | CA2385482C (enExample) |
| DE (1) | DE60025929T2 (enExample) |
| DK (1) | DK1221046T3 (enExample) |
| IL (1) | IL148650A0 (enExample) |
| NZ (1) | NZ518057A (enExample) |
| WO (1) | WO2001025769A2 (enExample) |
Families Citing this family (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
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| DE19948473A1 (de) | 1999-10-08 | 2001-04-12 | Nmi Univ Tuebingen | Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen |
| FR2802078B1 (fr) * | 1999-12-14 | 2003-10-03 | Univ Joseph Fourier | Microelectrode support de cellules a membrane excitable |
| CA2413663A1 (en) * | 2000-07-07 | 2002-01-17 | Charles D. Weaver | Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery |
| US7270730B2 (en) | 2000-08-04 | 2007-09-18 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
| US7067046B2 (en) | 2000-08-04 | 2006-06-27 | Essen Instruments, Inc. | System for rapid chemical activation in high-throughput electrophysiological measurements |
| WO2002024862A2 (en) * | 2000-09-19 | 2002-03-28 | Cytion S.A. | Sample positioning and analysis system |
| US6932893B2 (en) | 2000-10-02 | 2005-08-23 | Sophion Bioscience A/S | System for electrophysiological measurements |
| AU2001293676B2 (en) * | 2000-10-02 | 2006-07-27 | Sophion Bioscience A/S | System for electrophysiological measurements |
| US6776896B1 (en) | 2000-10-11 | 2004-08-17 | Axon Instruments, Inc. | Method of positioning cells for electrophysiological testing |
| EP1352952A1 (en) * | 2001-01-09 | 2003-10-15 | Matsushita Electric Industrial Co., Ltd. | Device for measuring extracellular potential, method of measuring extracellular potential by using the same and apparatus for quickly screening drug provided therewith |
| CA2441366A1 (en) | 2001-03-24 | 2002-10-03 | Aviva Biosciences Corporation | Biochips including ion transport detecting structures and methods of use |
| US20060029955A1 (en) | 2001-03-24 | 2006-02-09 | Antonio Guia | High-density ion transport measurement biochip devices and methods |
| US6899800B2 (en) | 2001-06-20 | 2005-05-31 | Axon Instruments, Inc. | Polymeric electrode for electrophysiological testing |
| CN1529814A (zh) * | 2001-06-20 | 2004-09-15 | 索菲昂生物科学有限公司 | 一种用于确定和/或监控离子通道电生理学性质的装置和方法 |
| JP2003043009A (ja) | 2001-07-30 | 2003-02-13 | Matsushita Electric Ind Co Ltd | 生体試料の発する物理化学的変化を検出するデバイスおよび装置 |
| DE10142393C1 (de) * | 2001-08-30 | 2003-01-23 | Fraunhofer Ges Forschung | Vorrichtung und Verfahren zur Erfassung bioelektrischer Signale aus elektrophysiologisch aktiven Bereichen in Sphäroiden |
| DE10202887B4 (de) * | 2002-01-25 | 2004-05-06 | Advalytix Ag | Zellanalyseverfahren |
| JP2005517180A (ja) * | 2002-02-05 | 2005-06-09 | ザ ユニヴァーシティー コート オブ ザ ユニヴァーシティー オブ グラスゴー | 細胞検定を実施するための装置 |
| WO2003068906A1 (en) | 2002-02-12 | 2003-08-21 | Cellectricon Ab | Systems and methods for rapidly changing the solution environment around sensors |
| US7470518B2 (en) | 2002-02-12 | 2008-12-30 | Cellectricon Ab | Systems and method for rapidly changing the solution environment around sensors |
| GB0207845D0 (en) * | 2002-04-04 | 2002-05-15 | Lgc Ltd | Apparatus, methods and means for biochemical analysis |
| GB2398635A (en) * | 2003-02-21 | 2004-08-25 | Sophion Bioscience As | A substrate providing a cell gigaseal for a patch clamp |
| EP1495105B1 (en) * | 2002-04-17 | 2007-02-21 | Sophion Bioscience A/S | Substrate and method for measuring the electrophysiological properties of cell membranes |
| AU2003231300A1 (en) * | 2002-05-04 | 2003-11-17 | Aviva Biosciences Corporation | Apparatus including ion transport detecting structures and methods of use |
| CN100370247C (zh) * | 2002-05-13 | 2008-02-20 | 松下电器产业株式会社 | 生物样本的活动信号测量装置和测量方法 |
| ES2208082B1 (es) * | 2002-05-29 | 2005-09-16 | Jose Luis Bardasano Rubio | Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables. |
| US8202439B2 (en) | 2002-06-05 | 2012-06-19 | Panasonic Corporation | Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm |
| JP4552423B2 (ja) * | 2003-11-21 | 2010-09-29 | パナソニック株式会社 | 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法 |
| JP3861831B2 (ja) * | 2003-03-07 | 2006-12-27 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| JP3945317B2 (ja) * | 2002-06-05 | 2007-07-18 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| WO2004079354A1 (ja) * | 2003-03-07 | 2004-09-16 | Matsushita Electric Industrial Co., Ltd. | 細胞外電位測定デバイスおよびその製造方法 |
| EP1411351A4 (en) | 2002-06-05 | 2010-07-07 | Panasonic Corp | DEVICE FOR MEASURING THE EXTRACELLULAR POTENTIAL AND METHOD FOR PRODUCING THE DEVICE |
| JP3925439B2 (ja) * | 2003-03-07 | 2007-06-06 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| JP3945338B2 (ja) * | 2002-08-01 | 2007-07-18 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| WO2006022092A1 (ja) | 2004-08-25 | 2006-03-02 | Matsushita Electric Industrial Co., Ltd. | 細胞電位測定プローブ |
| WO2003104455A1 (en) * | 2002-06-07 | 2003-12-18 | Sophion Bioscience A/S | Screening methods |
| US7470533B2 (en) | 2002-12-20 | 2008-12-30 | Acea Biosciences | Impedance based devices and methods for use in assays |
| US7468255B2 (en) | 2002-12-20 | 2008-12-23 | Acea Biosciences | Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology |
| AU2003269911A1 (en) | 2002-07-20 | 2004-02-09 | Acea Biosciences, Inc. | Impedance based apparatuses and methods for analyzing cells and particles |
| US8206903B2 (en) | 2002-12-20 | 2012-06-26 | Acea Biosciences | Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses |
| US8263375B2 (en) | 2002-12-20 | 2012-09-11 | Acea Biosciences | Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology |
| US7732127B2 (en) | 2002-12-20 | 2010-06-08 | Acea Biosciences, Inc. | Dynamic monitoring of cell adhesion and spreading using the RT-CES system |
| US7560269B2 (en) | 2002-12-20 | 2009-07-14 | Acea Biosciences, Inc. | Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays |
| US7810380B2 (en) | 2003-03-25 | 2010-10-12 | Tearlab Research, Inc. | Systems and methods for collecting tear film and measuring tear film osmolarity |
| EP1801586B1 (en) * | 2002-08-21 | 2010-10-13 | Cellectricon Ab | System for obtaining and maintaining high-resistance seals in patch clamp recordings |
| EP1546348A4 (en) * | 2002-08-21 | 2005-12-14 | Cellectricon Ab | SYSTEM AND METHOD FOR OBTAINING AND MAINTAINING HIGH-RESISTANCE JOINTS IN PATCH-CLAMP RECORDINGS |
| FR2844052B1 (fr) | 2002-08-28 | 2005-07-01 | Commissariat Energie Atomique | Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications |
| WO2004036202A1 (en) | 2002-10-16 | 2004-04-29 | Cellectricon Ab | Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells |
| US10215748B2 (en) | 2002-12-20 | 2019-02-26 | Acea Biosciences, Inc. | Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways |
| US11346797B2 (en) | 2002-12-20 | 2022-05-31 | Agilent Technologies, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties |
| US10539523B2 (en) | 2002-12-20 | 2020-01-21 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties |
| US10551371B2 (en) | 2003-11-10 | 2020-02-04 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function |
| US9612234B2 (en) | 2008-05-05 | 2017-04-04 | Acea Biosciences, Inc. | Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments |
| GB0303920D0 (en) | 2003-02-21 | 2003-03-26 | Sophion Bioscience As | Capillary stop |
| US7795039B2 (en) | 2003-06-10 | 2010-09-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Electronic device for communication with living cells |
| CA2550274A1 (en) | 2003-11-12 | 2005-05-26 | Acea Biosciences, Inc. | Real time electronic cell sensing systems and applications for cell-based assays |
| CA2556219A1 (en) * | 2004-02-09 | 2005-08-25 | Acea Biosciences, Inc. | Methods for assaying cells using cell-substrate impedance monitoring |
| FR2866958B1 (fr) | 2004-02-26 | 2006-08-04 | Commissariat Energie Atomique | Procede et dispositif de controle du positionnement d'un element biologique sur un support |
| GB2431013B (en) * | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| JP2006184207A (ja) * | 2004-12-28 | 2006-07-13 | Matsushita Electric Ind Co Ltd | 細胞膜の特性および状態の少なくとも一方の電気的測定方法および電気的測定装置 |
| US20060216203A1 (en) * | 2005-03-28 | 2006-09-28 | Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. | Multiwell sample plate with integrated impedance electrodes and connection scheme |
| EP1767929B1 (en) | 2005-06-07 | 2014-03-05 | Panasonic Corporation | Apparatus for measuring the electrical physiology of cells |
| JP4661539B2 (ja) * | 2005-11-11 | 2011-03-30 | パナソニック株式会社 | 細胞電気生理センサおよびその製造方法 |
| US7608417B2 (en) * | 2005-11-14 | 2009-10-27 | Panasonic Corporation | Cell electro-physiological sensor and method of manufacturing the same |
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| JP2009156572A (ja) * | 2006-04-06 | 2009-07-16 | National Institutes Of Natural Sciences | イオンチャンネルタンパク質バイオセンサー |
| JP4596009B2 (ja) | 2006-05-25 | 2010-12-08 | パナソニック株式会社 | 細胞電気生理センサ用チップとこれを用いた細胞電気生理センサおよび細胞電気生理センサ用チップの製造方法 |
| US8445263B2 (en) | 2006-07-06 | 2013-05-21 | Panasonic Corporation | Device for cellular electrophysiology sensor, cellular electrophysiology sensor using the device, and method for manufacturing the cellular electrophysiology sensor device |
| US8041515B2 (en) | 2006-09-20 | 2011-10-18 | Acea Biosciences, Inc. | Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents |
| EP2153224B1 (en) * | 2007-05-04 | 2012-12-19 | Tecella, LLC | Subsystem and method for use in patch clamp systems |
| JP5458887B2 (ja) | 2007-09-11 | 2014-04-02 | パナソニック株式会社 | シリコン構造体およびセンサチップ |
| EP2291645B1 (en) | 2008-05-05 | 2015-09-09 | Acea Biosciences, Inc. | Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution |
| US20130136673A1 (en) | 2010-07-09 | 2013-05-30 | Sophion Bioscience A/S | Chip assembly for use in a microfluidic analysis sytem |
| JP5575623B2 (ja) * | 2010-12-03 | 2014-08-20 | マルボシ酢株式会社 | 誘電特性測定用耐圧容器センサーに用いる絶縁体 |
| JP6037717B2 (ja) * | 2011-12-20 | 2016-12-07 | 国立研究開発法人科学技術振興機構 | プレーナーパッチクランプ装置、該装置用電極部及び細胞イオンチャンネル電流計測方法 |
| DK2802416T3 (en) * | 2012-01-09 | 2016-07-04 | Sophion Bioscience As | Enhanced cell adhesion in the patch area |
| DE102013007295B4 (de) * | 2013-04-26 | 2015-06-25 | Universität Rostock | Elektrophysiologische Messanordnung und elektrophysiologisches Messverfahren |
| CN105555945B (zh) | 2013-08-30 | 2018-05-04 | 国立研究开发法人科学技术振兴机构 | 平面膜片钳装置以及平面膜片钳系统 |
| BR112017005121A2 (pt) | 2014-09-23 | 2018-07-31 | Tearlab Res Inc | sistemas e métodos para integração de coleta de lágrima microfluídica e análise de fluxo lateral de analitos de interesse. |
| US12066428B2 (en) | 2015-11-20 | 2024-08-20 | Agilent Technologies, Inc. | Cell-substrate impedance monitoring of cancer cells |
| WO2017175879A1 (ja) * | 2016-04-05 | 2017-10-12 | シャープ株式会社 | センサ装置、検出方法、及びセンサユニット |
| JP6676486B2 (ja) | 2016-04-05 | 2020-04-08 | シャープ株式会社 | 検出方法 |
| US11746328B2 (en) | 2017-03-03 | 2023-09-05 | Agilent Technologies, Inc. | Methods and systems for functional maturation of iPSC and ESC derived cardiomyocytes |
| TWM565302U (zh) * | 2017-11-03 | 2018-08-11 | 國立臺灣大學 | 一種多離子感測電極陣列試片及其感測裝置 |
| JP7229110B2 (ja) | 2019-06-25 | 2023-02-27 | 株式会社Screenホールディングス | 細胞電位測定装置 |
| JP7469092B2 (ja) * | 2020-03-23 | 2024-04-16 | 株式会社Screenホールディングス | 細胞保持容器 |
| US20210301245A1 (en) | 2020-03-29 | 2021-09-30 | Agilent Technologies, Inc. | Systems and methods for electronically and optically monitoring biological samples |
| US20250076278A1 (en) | 2022-01-07 | 2025-03-06 | Sophion Bioscience A/S | Semi-automated patch-clamp apparatus and a method for carrying out a patch-clamp procedure |
| US20250083145A1 (en) | 2022-01-07 | 2025-03-13 | Sophion Bioscience A/S | Pipette guidance in multiple-well plate patch-clamp |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4062750A (en) * | 1974-12-18 | 1977-12-13 | James Francis Butler | Thin film electrochemical electrode and cell |
| US4225410A (en) * | 1978-12-04 | 1980-09-30 | Technicon Instruments Corporation | Integrated array of electrochemical sensors |
| US4874500A (en) * | 1987-07-15 | 1989-10-17 | Sri International | Microelectrochemical sensor and sensor array |
| US4812221A (en) * | 1987-07-15 | 1989-03-14 | Sri International | Fast response time microsensors for gaseous and vaporous species |
| DE4115414C2 (de) * | 1991-05-10 | 1995-07-06 | Meinhard Prof Dr Knoll | Verfahren zur Herstellung von miniaturisierten Chemo- und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente |
| IL103674A0 (en) * | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
| ATE205300T1 (de) * | 1997-12-17 | 2001-09-15 | Ecole Polytech | Positionierung und elektrophysiologische charakterisierung einzelner zellen und rekonstituierter membransysteme auf mikrostrukturierten trägern |
-
2000
- 2000-10-02 DK DK00962271T patent/DK1221046T3/da active
- 2000-10-02 WO PCT/DK2000/000548 patent/WO2001025769A2/en not_active Ceased
- 2000-10-02 NZ NZ518057A patent/NZ518057A/en not_active IP Right Cessation
- 2000-10-02 AT AT00962271T patent/ATE317545T1/de not_active IP Right Cessation
- 2000-10-02 CA CA2385482A patent/CA2385482C/en not_active Expired - Fee Related
- 2000-10-02 CN CNB008136157A patent/CN1204404C/zh not_active Expired - Fee Related
- 2000-10-02 IL IL14865000A patent/IL148650A0/xx unknown
- 2000-10-02 AU AU74065/00A patent/AU780157B2/en not_active Ceased
- 2000-10-02 JP JP2001528683A patent/JP4861584B2/ja not_active Expired - Lifetime
- 2000-10-02 DE DE60025929T patent/DE60025929T2/de not_active Expired - Lifetime
- 2000-10-02 EP EP00962271A patent/EP1221046B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1221046A2 (en) | 2002-07-10 |
| CA2385482C (en) | 2011-01-18 |
| CN1204404C (zh) | 2005-06-01 |
| IL148650A0 (en) | 2002-09-12 |
| DE60025929T2 (de) | 2006-11-09 |
| WO2001025769A2 (en) | 2001-04-12 |
| CN1377464A (zh) | 2002-10-30 |
| JP2003511668A (ja) | 2003-03-25 |
| AU780157B2 (en) | 2005-03-03 |
| NZ518057A (en) | 2004-01-30 |
| ATE317545T1 (de) | 2006-02-15 |
| AU7406500A (en) | 2001-05-10 |
| WO2001025769A3 (en) | 2001-08-30 |
| DK1221046T3 (da) | 2006-06-12 |
| CA2385482A1 (en) | 2001-04-12 |
| EP1221046B1 (en) | 2006-02-08 |
| DE60025929D1 (de) | 2006-04-20 |
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