DE60025929D1 - Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen - Google Patents

Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Info

Publication number
DE60025929D1
DE60025929D1 DE60025929T DE60025929T DE60025929D1 DE 60025929 D1 DE60025929 D1 DE 60025929D1 DE 60025929 T DE60025929 T DE 60025929T DE 60025929 T DE60025929 T DE 60025929T DE 60025929 D1 DE60025929 D1 DE 60025929D1
Authority
DE
Germany
Prior art keywords
measuring
cell
electrode
substrate
determining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60025929T
Other languages
English (en)
Other versions
DE60025929T2 (de
Inventor
Jon Wulff Petersen
Pieter Telleman
Ole Hansen
Palle Christophersen
Morten Bech
Peter Olesen
Jorgen Due
Lars Thomsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sophion Bioscience AS
Original Assignee
Sophion Bioscience AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sophion Bioscience AS filed Critical Sophion Bioscience AS
Application granted granted Critical
Publication of DE60025929D1 publication Critical patent/DE60025929D1/de
Publication of DE60025929T2 publication Critical patent/DE60025929T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60025929T 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen Expired - Lifetime DE60025929T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK140799 1999-10-01
DKPA199901407 1999-10-01
PCT/DK2000/000548 WO2001025769A2 (en) 1999-10-01 2000-10-02 A substrate and a method for determining and/or monitoring electrophysiological properties of ion channels

Publications (2)

Publication Number Publication Date
DE60025929D1 true DE60025929D1 (de) 2006-04-20
DE60025929T2 DE60025929T2 (de) 2006-11-09

Family

ID=8104506

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60025929T Expired - Lifetime DE60025929T2 (de) 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Country Status (11)

Country Link
EP (1) EP1221046B1 (de)
JP (1) JP4861584B2 (de)
CN (1) CN1204404C (de)
AT (1) ATE317545T1 (de)
AU (1) AU780157B2 (de)
CA (1) CA2385482C (de)
DE (1) DE60025929T2 (de)
DK (1) DK1221046T3 (de)
IL (1) IL148650A0 (de)
NZ (1) NZ518057A (de)
WO (1) WO2001025769A2 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19948473A1 (de) * 1999-10-08 2001-04-12 Nmi Univ Tuebingen Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen
FR2802078B1 (fr) * 1999-12-14 2003-10-03 Univ Joseph Fourier Microelectrode support de cellules a membrane excitable
CA2413663A1 (en) * 2000-07-07 2002-01-17 Charles D. Weaver Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
WO2002024862A2 (en) * 2000-09-19 2002-03-28 Cytion S.A. Sample positioning and analysis system
US6932893B2 (en) 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
CA2424498C (en) * 2000-10-02 2008-04-01 Sophion Bioscience A/S System for electrophysiological measurements
US6776896B1 (en) * 2000-10-11 2004-08-17 Axon Instruments, Inc. Method of positioning cells for electrophysiological testing
CN1458972A (zh) * 2001-01-09 2003-11-26 松下电器产业株式会社 测量细胞外电势的装置、利用该装置测量细胞外电势的方法以及配有该装置的快速筛选药物的仪器
CA2441366A1 (en) 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
US6899800B2 (en) 2001-06-20 2005-05-31 Axon Instruments, Inc. Polymeric electrode for electrophysiological testing
CN1529814A (zh) * 2001-06-20 2004-09-15 索菲昂生物科学有限公司 一种用于确定和/或监控离子通道电生理学性质的装置和方法
JP2003043009A (ja) * 2001-07-30 2003-02-13 Matsushita Electric Ind Co Ltd 生体試料の発する物理化学的変化を検出するデバイスおよび装置
DE10142393C1 (de) * 2001-08-30 2003-01-23 Fraunhofer Ges Forschung Vorrichtung und Verfahren zur Erfassung bioelektrischer Signale aus elektrophysiologisch aktiven Bereichen in Sphäroiden
DE10202887B4 (de) * 2002-01-25 2004-05-06 Advalytix Ag Zellanalyseverfahren
JP2005517180A (ja) * 2002-02-05 2005-06-09 ザ ユニヴァーシティー コート オブ ザ ユニヴァーシティー オブ グラスゴー 細胞検定を実施するための装置
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
EP2347824A3 (de) 2002-02-12 2012-03-07 Cellectricon Ab Systeme und Verfahren zur schnellen Änderung der Lösungsumgebung um Sensoren
GB0207845D0 (en) * 2002-04-04 2002-05-15 Lgc Ltd Apparatus, methods and means for biochemical analysis
EP1495105B1 (de) * 2002-04-17 2007-02-21 Sophion Bioscience A/S Substrat und verfahren zum messen elektrophysiologischer eigenschaften von zellmembranen
GB2398635A (en) 2003-02-21 2004-08-25 Sophion Bioscience As A substrate providing a cell gigaseal for a patch clamp
AU2003231300A1 (en) 2002-05-04 2003-11-17 Aviva Biosciences Corporation Apparatus including ion transport detecting structures and methods of use
WO2003096002A1 (fr) * 2002-05-13 2003-11-20 Matsushita Electric Industrial Co., Ltd. Instrument et procede de mesure du signal d'action d'un echantillon biologique
ES2208082B1 (es) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
JP4552423B2 (ja) * 2003-11-21 2010-09-29 パナソニック株式会社 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法
JP3945338B2 (ja) * 2002-08-01 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
WO2006132116A1 (ja) 2005-06-07 2006-12-14 Matsushita Electric Industrial Co., Ltd. 細胞電気生理測定デバイスおよびその製造方法
JP3925439B2 (ja) * 2003-03-07 2007-06-06 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
JP3945317B2 (ja) * 2002-06-05 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
CN100487456C (zh) 2002-06-05 2009-05-13 松下电器产业株式会社 细胞外电位测量装置及其制造方法
JP3861831B2 (ja) * 2003-03-07 2006-12-27 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
WO2007072790A1 (ja) 2005-12-20 2007-06-28 Matsushita Electric Industrial Co., Ltd. 細胞電気生理センサ
WO2006022092A1 (ja) 2004-08-25 2006-03-02 Matsushita Electric Industrial Co., Ltd. 細胞電位測定プローブ
DK1511843T3 (da) * 2002-06-07 2006-07-24 Sophion Bioscience As Screeningsmetoder
US7468255B2 (en) 2002-12-20 2008-12-23 Acea Biosciences Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
EP1539992B1 (de) * 2002-07-20 2009-11-11 Acea Biosciences, Inc. Vorrichtungen auf impedanzbasis sowie verfahren zur verwendung in assays
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7732127B2 (en) 2002-12-20 2010-06-08 Acea Biosciences, Inc. Dynamic monitoring of cell adhesion and spreading using the RT-CES system
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US7560269B2 (en) 2002-12-20 2009-07-14 Acea Biosciences, Inc. Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US7470533B2 (en) 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US7810380B2 (en) 2003-03-25 2010-10-12 Tearlab Research, Inc. Systems and methods for collecting tear film and measuring tear film osmolarity
EP1801586B1 (de) * 2002-08-21 2010-10-13 Cellectricon Ab System zur Gewinnung und Erhaltung hochresistenter Verschlüsse bei Patch-Clamp-Aufzeichnungen
DE60334580D1 (de) * 2002-08-21 2010-11-25 Cellectricon Ab System zur Gewinnung und Erhaltung hochresistenter Verschlüsse bei Patch-Clamp-Aufzeichnungen
FR2844052B1 (fr) 2002-08-28 2005-07-01 Commissariat Energie Atomique Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US9612234B2 (en) 2008-05-05 2017-04-04 Acea Biosciences, Inc. Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments
GB0303920D0 (en) 2003-02-21 2003-03-26 Sophion Bioscience As Capillary stop
EP1602922B1 (de) 2003-03-07 2016-01-13 Panasonic Intellectual Property Management Co., Ltd. Extrazellularpotential-messeinrichtung und verfahren zu ihrer herstellung
US7795039B2 (en) 2003-06-10 2010-09-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Electronic device for communication with living cells
CA2550274A1 (en) 2003-11-12 2005-05-26 Acea Biosciences, Inc. Real time electronic cell sensing systems and applications for cell-based assays
CA2556219A1 (en) * 2004-02-09 2005-08-25 Acea Biosciences, Inc. Methods for assaying cells using cell-substrate impedance monitoring
FR2866958B1 (fr) 2004-02-26 2006-08-04 Commissariat Energie Atomique Procede et dispositif de controle du positionnement d'un element biologique sur un support
GB2431013B (en) * 2004-07-23 2008-05-21 Electronic Bio Sciences Llc Method and apparatus for sensing a time varying current passing through an ion channel
JP2006184207A (ja) * 2004-12-28 2006-07-13 Matsushita Electric Ind Co Ltd 細胞膜の特性および状態の少なくとも一方の電気的測定方法および電気的測定装置
US20060216203A1 (en) * 2005-03-28 2006-09-28 Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. Multiwell sample plate with integrated impedance electrodes and connection scheme
JP4661539B2 (ja) * 2005-11-11 2011-03-30 パナソニック株式会社 細胞電気生理センサおよびその製造方法
US7608417B2 (en) * 2005-11-14 2009-10-27 Panasonic Corporation Cell electro-physiological sensor and method of manufacturing the same
JP2009156572A (ja) * 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
US8071363B2 (en) 2006-05-25 2011-12-06 Panasonic Corporation Chip for cell electrophysiological sensor, cell electrophysiological sensor using the same, and manufacturing method of chip for cell electrophysiological sensor
WO2008004476A1 (fr) 2006-07-06 2008-01-10 Panasonic Corporation Dispositif pour capteur cellulaire électrophysiologique, capteur cellulaire électrophysiologique utilisant le dispositif, et procédé de fabrication du dispositif pour capteur cellulaire électrophysiologique
US8041515B2 (en) 2006-09-20 2011-10-18 Acea Biosciences, Inc. Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
US8163527B2 (en) * 2007-05-04 2012-04-24 Tecella Llc Subsystems and methods for use in patch clamp systems
US8314466B2 (en) 2007-09-11 2012-11-20 Panasonic Corporation Silicon structure, method for manufacturing the same, and sensor chip
EP2291645B1 (de) 2008-05-05 2015-09-09 Acea Biosciences, Inc. Markierungsfreie überwachung der elektromechanischen kopplung und erregbaren zellen anhand von systemen auf impedanzbasis mit millisekunden-zeitauflösung
WO2012004297A1 (en) 2010-07-09 2012-01-12 Sophion Bioscience A/S A chip assembly for use in a microfluidic analysis system
JP5575623B2 (ja) * 2010-12-03 2014-08-20 マルボシ酢株式会社 誘電特性測定用耐圧容器センサーに用いる絶縁体
JP6037717B2 (ja) 2011-12-20 2016-12-07 国立研究開発法人科学技術振興機構 プレーナーパッチクランプ装置、該装置用電極部及び細胞イオンチャンネル電流計測方法
WO2013104608A1 (en) * 2012-01-09 2013-07-18 Sophion Bioscience A/S Improved patch area cell adhesion
DE102013007295B4 (de) * 2013-04-26 2015-06-25 Universität Rostock Elektrophysiologische Messanordnung und elektrophysiologisches Messverfahren
TW201536915A (zh) 2013-08-30 2015-10-01 Japan Science & Tech Agency 平面膜片箝制裝置及平面膜片箝制系統
ES2828278T3 (es) 2014-09-23 2021-05-25 Tearlab Res Inc Sistema de integración de recolección de lágrimas microfluídicas y análisis de flujo lateral de analitos de interés
JP6676486B2 (ja) 2016-04-05 2020-04-08 シャープ株式会社 検出方法
WO2017175879A1 (ja) * 2016-04-05 2017-10-12 シャープ株式会社 センサ装置、検出方法、及びセンサユニット
EP3589299A4 (de) 2017-03-03 2021-01-13 ACEA Biosciences, Inc. Verfahren und systeme zur funktionellen reifung von kardiomyozyten aus ipsc und esc
TWM565302U (zh) * 2017-11-03 2018-08-11 國立臺灣大學 一種多離子感測電極陣列試片及其感測裝置
JP7229110B2 (ja) * 2019-06-25 2023-02-27 株式会社Screenホールディングス 細胞電位測定装置
JP7469092B2 (ja) * 2020-03-23 2024-04-16 株式会社Screenホールディングス 細胞保持容器
WO2023131694A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Pipette guidance in multiple-well plate patch-clamp
WO2023131698A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Semi-automated patch-clamp apparatus and a method for carrying out a patch-clamp procedure

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062750A (en) * 1974-12-18 1977-12-13 James Francis Butler Thin film electrochemical electrode and cell
US4225410A (en) * 1978-12-04 1980-09-30 Technicon Instruments Corporation Integrated array of electrochemical sensors
US4874500A (en) * 1987-07-15 1989-10-17 Sri International Microelectrochemical sensor and sensor array
US4812221A (en) * 1987-07-15 1989-03-14 Sri International Fast response time microsensors for gaseous and vaporous species
DE4115414C2 (de) * 1991-05-10 1995-07-06 Meinhard Prof Dr Knoll Verfahren zur Herstellung von miniaturisierten Chemo- und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente
IL103674A0 (en) * 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
AU746580B2 (en) * 1997-12-17 2002-05-02 Ecole Polytechnique Federale De Lausanne Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers

Also Published As

Publication number Publication date
NZ518057A (en) 2004-01-30
JP2003511668A (ja) 2003-03-25
WO2001025769A3 (en) 2001-08-30
CA2385482A1 (en) 2001-04-12
JP4861584B2 (ja) 2012-01-25
IL148650A0 (en) 2002-09-12
EP1221046B1 (de) 2006-02-08
CA2385482C (en) 2011-01-18
EP1221046A2 (de) 2002-07-10
DK1221046T3 (da) 2006-06-12
DE60025929T2 (de) 2006-11-09
ATE317545T1 (de) 2006-02-15
WO2001025769A2 (en) 2001-04-12
CN1377464A (zh) 2002-10-30
AU780157B2 (en) 2005-03-03
AU7406500A (en) 2001-05-10
CN1204404C (zh) 2005-06-01

Similar Documents

Publication Publication Date Title
DE60025929D1 (de) Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen
JP3093274B2 (ja) 気体濃度測定の方法及びそれを実施するための微細加工検出装置
DE60330385D1 (de) Verfahren und vorrichtung zum einfangen geladener moleküle eines stromes
KR0171227B1 (ko) 가스 센서
WO2005026178A3 (en) Electrochemical affinity biosensor system and methods
GB0801142D0 (en) Label-free molecule detection and measurement
DE60220035D1 (de) Sensor-array-vorrichtung sowie ein verfahren zur überwachung von elektrochemischer korrosion
TWI609182B (zh) 葡萄糖量測裝置與設備
DE60329187D1 (de) Impedanz basierende vorrichtungen und verfahren zur analyse von zellen und partikeln
CN104737010B (zh) 生物体由来物的检查装置
US20130168247A1 (en) Ion analyzer
TWI625522B (zh) 平面型氨選擇性感測電極及其製法
EP0712492B1 (de) Brennstoffzellen
CN109612921A (zh) 一种腐蚀监测传感器及其制备方法
JP2008298644A (ja) 電気泳動兼転写装置、電気泳動兼転写用チップ、ならびに電気泳動および転写方法
JP7330519B2 (ja) 生物学的または化学的因子試料のインピーダンス測定のためのセンサ、およびこのようなセンサを使用する、試料における生物学的または化学的因子の検出方法
US7652479B2 (en) Electrolyte measurement device and measurement procedure
WO2009008334A1 (ja) イオン伝導性電解質膜の検査方法及び装置
US20120111738A1 (en) Gas sensor testing device
JPS61176846A (ja) イオン濃度測定方法
RU197029U1 (ru) Электродиализатор
RU201055U1 (ru) Ячейка для измерения проводимости и окна электрохимической стабильности жидких электролитов
KR102294708B1 (ko) 중금속 이온류 검출용 가변전압 기반의 스트립 센서, 그 제조방법 및 이를 이용한 중금속 이온류 동시 검출방법
JPH07119737B2 (ja) 溶質濃度測定装置
DE60327099D1 (de) Sonde zur bestimmung der sauerstoffaktivität in metallschmelzen und methode zu deren herstellung

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: STROHSCHAENK UND KOLLEGEN, 85521 OTTOBRUNN