ATE317545T1 - Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen - Google Patents

Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Info

Publication number
ATE317545T1
ATE317545T1 AT00962271T AT00962271T ATE317545T1 AT E317545 T1 ATE317545 T1 AT E317545T1 AT 00962271 T AT00962271 T AT 00962271T AT 00962271 T AT00962271 T AT 00962271T AT E317545 T1 ATE317545 T1 AT E317545T1
Authority
AT
Austria
Prior art keywords
measuring
cell
electrode
substrate
determining
Prior art date
Application number
AT00962271T
Other languages
English (en)
Inventor
Jon Wulff Petersen
Pieter Telleman
Ole Hansen
Palle Christophersen
Morten Bech
Soren Peter Olesen
Jorgen Due
Lars Thomsen
Original Assignee
Sophion Bioscience As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sophion Bioscience As filed Critical Sophion Bioscience As
Application granted granted Critical
Publication of ATE317545T1 publication Critical patent/ATE317545T1/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00962271T 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen ATE317545T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA199901407 1999-10-01

Publications (1)

Publication Number Publication Date
ATE317545T1 true ATE317545T1 (de) 2006-02-15

Family

ID=8104506

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00962271T ATE317545T1 (de) 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Country Status (11)

Country Link
EP (1) EP1221046B1 (de)
JP (1) JP4861584B2 (de)
CN (1) CN1204404C (de)
AT (1) ATE317545T1 (de)
AU (1) AU780157B2 (de)
CA (1) CA2385482C (de)
DE (1) DE60025929T2 (de)
DK (1) DK1221046T3 (de)
IL (1) IL148650A0 (de)
NZ (1) NZ518057A (de)
WO (1) WO2001025769A2 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19948473A1 (de) * 1999-10-08 2001-04-12 Nmi Univ Tuebingen Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen
FR2802078B1 (fr) * 1999-12-14 2003-10-03 Univ Joseph Fourier Microelectrode support de cellules a membrane excitable
WO2002004943A2 (en) * 2000-07-07 2002-01-17 Bristol-Myers Squibb Company Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
AU2001287472A1 (en) * 2000-09-19 2002-04-02 Cytion Sa Sample positioning and analysis system
WO2002029402A2 (en) * 2000-10-02 2002-04-11 Sophion Bioscience A/S System for electrophysiological measurements
US6932893B2 (en) 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
US6776896B1 (en) * 2000-10-11 2004-08-17 Axon Instruments, Inc. Method of positioning cells for electrophysiological testing
KR20020086625A (ko) * 2001-01-09 2002-11-18 마쯔시다덴기산교 가부시키가이샤 세포외 전위 측정용 디바이스, 그것을 사용한 세포외 전위측정 방법 및 그것을 구비한 고속 약품 스크리닝 장치
WO2002077259A2 (en) 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
US6899800B2 (en) 2001-06-20 2005-05-31 Axon Instruments, Inc. Polymeric electrode for electrophysiological testing
WO2002103354A1 (en) * 2001-06-20 2002-12-27 Sophion Bioscience A/S An apparatus and method for determining and/or monitoring electrophysiological properties of ion channels
JP2003043009A (ja) 2001-07-30 2003-02-13 Matsushita Electric Ind Co Ltd 生体試料の発する物理化学的変化を検出するデバイスおよび装置
DE10142393C1 (de) * 2001-08-30 2003-01-23 Fraunhofer Ges Forschung Vorrichtung und Verfahren zur Erfassung bioelektrischer Signale aus elektrophysiologisch aktiven Bereichen in Sphäroiden
DE10202887B4 (de) * 2002-01-25 2004-05-06 Advalytix Ag Zellanalyseverfahren
US8003372B2 (en) 2002-02-05 2011-08-23 The University Court Of The University Of Glasgow Device for performing cell assays
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
EP2368635A3 (de) 2002-02-12 2012-03-07 Cellectricon Ab Systeme und Verfahren zur schnellen Änderung der Lösungsumgebung um Sensoren
US7390650B2 (en) 2002-08-21 2008-06-24 Cellectricon Ab System and method for obtaining and maintaining high-resistance seals in patch clamp recordings
GB0207845D0 (en) * 2002-04-04 2002-05-15 Lgc Ltd Apparatus, methods and means for biochemical analysis
GB2398635A (en) * 2003-02-21 2004-08-25 Sophion Bioscience As A substrate providing a cell gigaseal for a patch clamp
EP1495105B1 (de) * 2002-04-17 2007-02-21 Sophion Bioscience A/S Substrat und verfahren zum messen elektrophysiologischer eigenschaften von zellmembranen
EP1501924A4 (de) * 2002-05-04 2006-05-24 Aviva Biosciences Corp Vorrichtung mit strukturen nachweisendem ionentransport und verwendungsverfahren
AU2003234787A1 (en) 2002-05-13 2003-11-11 Matsushita Electric Industrial Co., Ltd. Instrument and method for measuring action signal of biological sample
ES2208082B1 (es) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
CN100487456C (zh) 2002-06-05 2009-05-13 松下电器产业株式会社 细胞外电位测量装置及其制造方法
JP4552423B2 (ja) * 2003-11-21 2010-09-29 パナソニック株式会社 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法
WO2009034697A1 (ja) 2007-09-11 2009-03-19 Panasonic Corporation シリコン構造体およびその製造方法並びにセンサチップ
WO2007138902A1 (ja) 2006-05-25 2007-12-06 Panasonic Corporation 細胞電気生理センサ用チップとこれを用いた細胞電気生理センサおよび細胞電気生理センサ用チップの製造方法
JP3925439B2 (ja) * 2003-03-07 2007-06-06 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
JP3945338B2 (ja) * 2002-08-01 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
WO2004079354A1 (ja) * 2003-03-07 2004-09-16 Matsushita Electric Industrial Co., Ltd. 細胞外電位測定デバイスおよびその製造方法
JP3861831B2 (ja) * 2003-03-07 2006-12-27 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
JP3945317B2 (ja) * 2002-06-05 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
AU2003251124A1 (en) * 2002-06-07 2003-12-22 Sophion Bioscience A/S Screening methods
US7468255B2 (en) 2002-12-20 2008-12-23 Acea Biosciences Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
US7470533B2 (en) 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US7732127B2 (en) 2002-12-20 2010-06-08 Acea Biosciences, Inc. Dynamic monitoring of cell adhesion and spreading using the RT-CES system
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7192752B2 (en) 2002-12-20 2007-03-20 Acea Biosciences Real time electronic cell sensing systems and applications for cell-based assays
US7560269B2 (en) 2002-12-20 2009-07-14 Acea Biosciences, Inc. Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
JP4745056B2 (ja) * 2002-07-20 2011-08-10 アセア バイオサイエンシーズ，インク． インピーダンスによる細胞と微粒子の測定装置及び方法
US7810380B2 (en) 2003-03-25 2010-10-12 Tearlab Research, Inc. Systems and methods for collecting tear film and measuring tear film osmolarity
EP1801586B1 (de) * 2002-08-21 2010-10-13 Cellectricon Ab System zur Gewinnung und Erhaltung hochresistenter Verschlüsse bei Patch-Clamp-Aufzeichnungen
FR2844052B1 (fr) 2002-08-28 2005-07-01 Commissariat Energie Atomique Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
GB0303920D0 (en) * 2003-02-21 2003-03-26 Sophion Bioscience As Capillary stop
JP4728956B2 (ja) 2003-06-10 2011-07-20 イサム リサーチ デベロップメント カンパニー オブ ザ ヘブルー ユニバーシティ オブ エルサレム 生体細胞との通信のための電子装置
EP1749203A4 (de) * 2004-02-09 2009-05-06 Xiao Xu Elektronisches echtzeit-zellenerfassungssystem und anwendungen für die cytotoxizitätsprofilierung und zusammengesetzte assays
FR2866958B1 (fr) 2004-02-26 2006-08-04 Commissariat Energie Atomique Procede et dispositif de controle du positionnement d'un element biologique sur un support
GB2431013B (en) * 2004-07-23 2008-05-21 Electronic Bio Sciences Llc Method and apparatus for sensing a time varying current passing through an ion channel
JPWO2006022092A1 (ja) 2004-08-25 2008-05-08 松下電器産業株式会社 細胞電位測定プローブ
JP2006184207A (ja) * 2004-12-28 2006-07-13 Matsushita Electric Ind Co Ltd 細胞膜の特性および状態の少なくとも一方の電気的測定方法および電気的測定装置
US20060216203A1 (en) * 2005-03-28 2006-09-28 Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. Multiwell sample plate with integrated impedance electrodes and connection scheme
US8318477B2 (en) 2005-06-07 2012-11-27 Panasonic Corporation Cellular electrophysiological measurement device and method for manufacturing the same
JP4661539B2 (ja) * 2005-11-11 2011-03-30 パナソニック株式会社 細胞電気生理センサおよびその製造方法
US7608417B2 (en) * 2005-11-14 2009-10-27 Panasonic Corporation Cell electro-physiological sensor and method of manufacturing the same
JP4470999B2 (ja) 2005-12-20 2010-06-02 パナソニック株式会社 細胞電気生理センサ
JP2009156572A (ja) * 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
WO2008004476A1 (fr) 2006-07-06 2008-01-10 Panasonic Corporation Dispositif pour capteur cellulaire électrophysiologique, capteur cellulaire électrophysiologique utilisant le dispositif, et procédé de fabrication du dispositif pour capteur cellulaire électrophysiologique
US8041515B2 (en) 2006-09-20 2011-10-18 Acea Biosciences, Inc. Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
JP5393657B2 (ja) * 2007-05-04 2014-01-22 テセラ， エルエルシー パッチクランプシステムにおける使用のためのサブシステムおよび方法
CA2723223C (en) 2008-05-05 2017-06-06 Acea Biosciences, Inc. Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
WO2011146531A1 (en) 2010-05-18 2011-11-24 Acea Biosciences, Inc Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (rtca) cardio instruments
US20130136673A1 (en) 2010-07-09 2013-05-30 Sophion Bioscience A/S Chip assembly for use in a microfluidic analysis sytem
JP5575623B2 (ja) * 2010-12-03 2014-08-20 マルボシ酢株式会社 誘電特性測定用耐圧容器センサーに用いる絶縁体
JP6037717B2 (ja) * 2011-12-20 2016-12-07 国立研究開発法人科学技術振興機構 プレーナーパッチクランプ装置、該装置用電極部及び細胞イオンチャンネル電流計測方法
WO2013104608A1 (en) * 2012-01-09 2013-07-18 Sophion Bioscience A/S Improved patch area cell adhesion
DE102013007295B4 (de) * 2013-04-26 2015-06-25 Universität Rostock Elektrophysiologische Messanordnung und elektrophysiologisches Messverfahren
US9977009B2 (en) 2013-08-30 2018-05-22 Japan Science And Technology Agency Planar patch clamp device and planar patch clamp system
MX2017003454A (es) 2014-09-23 2017-11-13 Tearlab Res Inc Sistemas y metodos para la integracion de la recoleccion de microfluido lagrimal y el analisis por flujo lateral de analitos de interes.
US12066428B2 (en) 2015-11-20 2024-08-20 Agilent Technologies, Inc. Cell-substrate impedance monitoring of cancer cells
JP6676486B2 (ja) * 2016-04-05 2020-04-08 シャープ株式会社 検出方法
WO2017175879A1 (ja) * 2016-04-05 2017-10-12 シャープ株式会社 センサ装置、検出方法、及びセンサユニット
CN110582569B (zh) 2017-03-03 2024-04-02 安捷伦科技有限公司 用于iPSC和ESC衍生的心肌细胞的功能成熟的方法和系统
TWI695166B (zh) * 2017-11-03 2020-06-01 國立臺灣大學 一種多離子感測電極陣列試片及其感測裝置
JP7229110B2 (ja) 2019-06-25 2023-02-27 株式会社Ｓｃｒｅｅｎホールディングス 細胞電位測定装置
JP7469092B2 (ja) * 2020-03-23 2024-04-16 株式会社Ｓｃｒｅｅｎホールディングス 細胞保持容器
US20210301245A1 (en) 2020-03-29 2021-09-30 Agilent Technologies, Inc. Systems and methods for electronically and optically monitoring biological samples
WO2023131698A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Semi-automated patch-clamp apparatus and a method for carrying out a patch-clamp procedure
WO2023131694A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Pipette guidance in multiple-well plate patch-clamp

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062750A (en) * 1974-12-18 1977-12-13 James Francis Butler Thin film electrochemical electrode and cell
US4225410A (en) * 1978-12-04 1980-09-30 Technicon Instruments Corporation Integrated array of electrochemical sensors
US4812221A (en) * 1987-07-15 1989-03-14 Sri International Fast response time microsensors for gaseous and vaporous species
US4874500A (en) * 1987-07-15 1989-10-17 Sri International Microelectrochemical sensor and sensor array
DE4115414C2 (de) * 1991-05-10 1995-07-06 Meinhard Prof Dr Knoll Verfahren zur Herstellung von miniaturisierten Chemo- und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente
IL103674A0 (en) * 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
WO1999031503A1 (de) * 1997-12-17 1999-06-24 Horst Vogel Positionierung und elektrophysiologische charakterisierung einzelner zellen und rekonstituierter membransysteme auf mikrostrukturierten trägern

Also Published As

Publication number Publication date
CA2385482A1 (en) 2001-04-12
DE60025929T2 (de) 2006-11-09
JP2003511668A (ja) 2003-03-25
WO2001025769A3 (en) 2001-08-30
CN1377464A (zh) 2002-10-30
DK1221046T3 (da) 2006-06-12
EP1221046B1 (de) 2006-02-08
DE60025929D1 (de) 2006-04-20
IL148650A0 (en) 2002-09-12
CN1204404C (zh) 2005-06-01
WO2001025769A2 (en) 2001-04-12
AU7406500A (en) 2001-05-10
CA2385482C (en) 2011-01-18
AU780157B2 (en) 2005-03-03
JP4861584B2 (ja) 2012-01-25
NZ518057A (en) 2004-01-30
EP1221046A2 (de) 2002-07-10

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