JP4801258B2 - アフィニティ分離の方法及びそれに用いるリガンド - Google Patents

アフィニティ分離の方法及びそれに用いるリガンド Download PDF

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JP4801258B2
JP4801258B2 JP2000577291A JP2000577291A JP4801258B2 JP 4801258 B2 JP4801258 B2 JP 4801258B2 JP 2000577291 A JP2000577291 A JP 2000577291A JP 2000577291 A JP2000577291 A JP 2000577291A JP 4801258 B2 JP4801258 B2 JP 4801258B2
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protein
asn
affinity
binding
residues
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JP2002527107A (ja
JP2002527107A5 (https=
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ユーレン、マーチアス
ホバー、ソフィア
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000577291A 1998-10-21 1999-10-21 アフィニティ分離の方法及びそれに用いるリガンド Expired - Lifetime JP4801258B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9823071.7 1998-10-21
GBGB9823071.7A GB9823071D0 (en) 1998-10-21 1998-10-21 A method
PCT/GB1999/003484 WO2000023580A1 (en) 1998-10-21 1999-10-21 A method of affinity separation and ligands for use therein

Publications (3)

Publication Number Publication Date
JP2002527107A JP2002527107A (ja) 2002-08-27
JP2002527107A5 JP2002527107A5 (https=) 2006-12-07
JP4801258B2 true JP4801258B2 (ja) 2011-10-26

Family

ID=10841043

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JP2000577291A Expired - Lifetime JP4801258B2 (ja) 1998-10-21 1999-10-21 アフィニティ分離の方法及びそれに用いるリガンド

Country Status (12)

Country Link
US (2) US6831161B1 (https=)
EP (2) EP1672068A1 (https=)
JP (1) JP4801258B2 (https=)
AT (1) ATE305970T1 (https=)
AU (1) AU773368B2 (https=)
CA (2) CA2347960C (https=)
DE (1) DE69927598T2 (https=)
DK (1) DK1123389T3 (https=)
GB (1) GB9823071D0 (https=)
NO (1) NO323994B1 (https=)
WO (1) WO2000023580A1 (https=)
ZA (1) ZA200103252B (https=)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7709209B2 (en) 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
SE0200943D0 (sv) 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
GB0214007D0 (en) * 2002-06-18 2002-07-31 Common Services Agency Removal of prion infectivity
AU2004245429A1 (en) * 2003-06-10 2004-12-16 Farallone Holding Bv Binding peptides: methods for their generation and use
SE0301936D0 (sv) * 2003-06-30 2003-06-30 Affibody Ab New polypeptide
CA2531238C (en) * 2003-07-04 2015-02-24 Affibody Ab Polypeptides having binding affinity for her2
JP5634008B2 (ja) 2004-04-06 2014-12-03 アフィボディ・アーベー 新規の使用および方法
WO2008039141A1 (en) 2006-09-29 2008-04-03 Ge Healthcare Bio-Sciences Ab Chromatography ligand comprising domain c from staphyloccocus aureus protein a for antibody isolation
EP2190863B1 (en) * 2007-07-31 2015-09-02 Affibody AB New albumin binding compositions, methods and uses
GB2452301A (en) 2007-08-30 2009-03-04 Ge Healthcare Bio Sciences Ab Sterilization Method
US8592555B2 (en) * 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
CN102165312B (zh) * 2008-09-25 2015-04-22 Jsr株式会社 亲合层析用填充剂
JP5229888B2 (ja) * 2008-09-30 2013-07-03 独立行政法人産業技術総合研究所 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
JPWO2010110288A1 (ja) 2009-03-24 2012-09-27 株式会社カネカ 免疫グロブリンに親和性を有するタンパク質および免疫グロブリン結合性アフィニティーリガンド
WO2011118699A1 (ja) 2010-03-24 2011-09-29 株式会社カネカ 免疫グロブリンに特異的に結合するタンパク質および免疫グロブリン結合性アフィニティーリガンド
WO2012083425A1 (en) * 2010-12-21 2012-06-28 The University Of Western Ontario Novel alkali-resistant variants of protein a and their use in affinity chromatography
US9040661B2 (en) 2010-12-21 2015-05-26 Jsr Corporation Support for affinity chromatography and method for isolating immunoglobulin
US9273151B2 (en) 2011-03-24 2016-03-01 Kaneka Corporation Proteinaceous-substance-binding low-molecular-weight compound
EP2690108B1 (en) 2011-03-25 2018-07-25 Kaneka Corporation Novel immunoglobulin-binding polypeptide
SG186552A1 (en) 2011-06-08 2013-01-30 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
WO2014076179A1 (en) 2012-11-14 2014-05-22 Affibody Ab New polypeptide
WO2014076177A1 (en) * 2012-11-14 2014-05-22 Affibody Ab New polypeptide
USRE48805E1 (en) 2013-03-06 2021-11-02 Vision Global Holdings Ltd. Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein
US9255262B2 (en) * 2013-03-06 2016-02-09 Vision Global Holdings Ltd. Albumin-binding arginine deminase and the use thereof
CN105518020A (zh) 2013-09-04 2016-04-20 Emd密理博公司 清洗基于a蛋白的亲和色谱柱的方法
US10221211B2 (en) 2013-09-27 2019-03-05 Kaneka Corporation Process for producing porous cellulose beads using alkali aqueous solution
JP6326499B2 (ja) 2013-12-12 2018-05-16 イー・エム・デイー・ミリポア・コーポレイシヨン アクリルアミド含有フィルターを使用したタンパク質分離
JP6369807B2 (ja) * 2014-10-21 2018-08-08 株式会社プロテイン・エクスプレス プロテインｌ変異体
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
CN108064286A (zh) 2015-01-26 2018-05-22 株式会社钟化 突变型免疫球蛋白κ链可变区结合性肽
JP6705806B2 (ja) 2015-03-26 2020-06-03 Jsr株式会社 イムノグロブリン結合タンパク質およびそれを用いたアフィニティー担体
CN121159719A (zh) 2015-04-17 2025-12-19 高山免疫科学股份有限公司 具有可调的亲和力的免疫调节蛋白
EP3375873B1 (en) 2015-10-22 2025-07-02 Protenova Co., Ltd. Immunoglobulin-binding polypeptide
WO2017191748A1 (ja) * 2016-05-02 2017-11-09 株式会社カネカ 改変型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
EP3592759A1 (en) 2017-03-07 2020-01-15 JMVA Biotech AB Modified adsorptive surfaces
JP7159175B2 (ja) 2017-09-25 2022-10-24 Jsr株式会社 イムノグロブリン結合タンパク質、及びそれを用いたアフィニティー担体
KR20260046525A (ko) 2018-04-11 2026-04-07 인히브릭스 바이오사이언스, 인크. 제한된 cd3 결합을 갖는 다중특이적 폴리펩티드 컨스트럭트 및 관련된 방법 및 용도
EP3842536A4 (en) 2018-08-24 2023-01-18 JSR Corporation IMMUNOGLOBULIN-BINDING PROTEIN AND AFFINITY CARRIER WITH IT
WO2022081779A1 (en) 2020-10-13 2022-04-21 Avitide LLC Affinity ligand libraries of three-helix bundle proteins and uses thereof
US20240043478A1 (en) 2020-12-22 2024-02-08 Protenova Co., Ltd. Immunoglobulin-binding polypeptide
EP4294542A1 (en) 2021-02-19 2023-12-27 Avitide LLC Aav2 affinity agents
GB2609185A (en) 2021-05-24 2023-02-01 Astrea Uk Services Ltd Scaffold for isolation of a biomolecule
EP4541814A1 (en) * 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Stabilized polypeptides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462046C (sv) * 1982-08-23 1998-04-27 Pharmacia Biotech Ab Hybrid-DNA-vektor innehållande DNA-sekvens från Staphylococcus aureus, förfarande för dess framställning och bakterie innehållande densamma
US5401832A (en) * 1984-12-24 1995-03-28 Merck & Co., Inc. Brain derived and recombinant acidic fibroblast growth factor
US5089473A (en) * 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
JP2584697B2 (ja) * 1991-08-23 1997-02-26 オリエンタル酵母工業株式会社 ＩｇＧ精製用組換え型プロテインＡ
EP0550771B1 (en) * 1991-07-25 1999-09-29 Oriental Yeast Co., Ltd. Immunoglobulin-binding artificial protein
ATE154526T1 (de) * 1992-04-16 1997-07-15 Merck Patent Gmbh Aktivierte trägermaterialien, ihre herstellung und verwendung
SE9400088D0 (sv) * 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
EP0968290B1 (en) * 1997-03-12 2004-12-15 SmithKline Beecham Corporation Anti-alphavbeta3 humanized monoclonal antibodies

Also Published As

Publication number Publication date
CA2633345A1 (en) 2000-04-27
NO20011969L (no) 2001-06-19
NO323994B1 (no) 2007-07-30
CA2633345C (en) 2013-02-26
US6831161B1 (en) 2004-12-14
CA2347960C (en) 2008-08-05
US20050100970A1 (en) 2005-05-12
GB9823071D0 (en) 1998-12-16
DK1123389T3 (da) 2006-01-16
EP1123389B1 (en) 2005-10-05
EP1123389A1 (en) 2001-08-16
EP1672068A1 (en) 2006-06-21
JP2002527107A (ja) 2002-08-27
WO2000023580A1 (en) 2000-04-27
NO20011969D0 (no) 2001-04-20
AU773368B2 (en) 2004-05-20
DE69927598D1 (de) 2006-02-16
CA2347960A1 (en) 2000-04-27
DE69927598T2 (de) 2006-07-13
AU6353299A (en) 2000-05-08
ZA200103252B (en) 2002-07-22
ATE305970T1 (de) 2005-10-15

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