JP4755422B2 - ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 - Google Patents
ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 Download PDFInfo
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- A61K2039/5256—Virus expressing foreign proteins
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- A61K2039/53—DNA (RNA) vaccination
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- A61K2039/585—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation wherein the target is cancer
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- A—HUMAN NECESSITIES
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- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/80—Vaccine for a specifically defined cancer
- A61K2039/892—Reproductive system [uterus, ovaries, cervix, testes]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C12N2710/00011—Details
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- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12N2710/00011—Details
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- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
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- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
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- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36141—Use of virus, viral particle or viral elements as a vector
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| US41592902P | 2002-10-03 | 2002-10-03 | |
| US60/415,929 | 2002-10-03 | ||
| PCT/US2003/031726 WO2004030636A2 (en) | 2002-10-03 | 2003-10-02 | Human papillomavirus polypeptides and immunogenic compositions |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2010181424A Division JP5398665B2 (ja) | 2002-10-03 | 2010-08-13 | ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 |
Publications (3)
| Publication Number | Publication Date |
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| JP2006501825A JP2006501825A (ja) | 2006-01-19 |
| JP2006501825A5 JP2006501825A5 (https=) | 2006-11-16 |
| JP4755422B2 true JP4755422B2 (ja) | 2011-08-24 |
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| JP2010181424A Expired - Lifetime JP5398665B2 (ja) | 2002-10-03 | 2010-08-13 | ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 |
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| JP2010181424A Expired - Lifetime JP5398665B2 (ja) | 2002-10-03 | 2010-08-13 | ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 |
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| US (2) | US7223408B2 (https=) |
| EP (1) | EP1553966B1 (https=) |
| JP (2) | JP4755422B2 (https=) |
| KR (1) | KR101145190B1 (https=) |
| CN (1) | CN1720060B (https=) |
| AU (1) | AU2003282731B2 (https=) |
| BR (1) | BR0314529A (https=) |
| CA (1) | CA2499837C (https=) |
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| DK (1) | DK1553966T3 (https=) |
| ES (1) | ES2391451T3 (https=) |
| MX (1) | MXPA05003395A (https=) |
| NZ (1) | NZ556544A (https=) |
| PT (1) | PT1553966E (https=) |
| WO (1) | WO2004030636A2 (https=) |
| ZA (1) | ZA200502618B (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010279386A (ja) * | 2002-10-03 | 2010-12-16 | Wyeth Holdings Corp | ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 |
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| US8128922B2 (en) | 1999-10-20 | 2012-03-06 | Johns Hopkins University | Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen |
| JP5087201B2 (ja) | 2000-08-03 | 2012-12-05 | ジョンズ・ホプキンス・ユニバーシティ | 抗原に小胞体シャペロンポリペプチドを連結した分子ワクチン |
| US9701725B2 (en) | 2003-05-05 | 2017-07-11 | The Johns Hopkins University | Anti-cancer DNA vaccine employing plasmids encoding signal sequence, mutant oncoprotein antigen, and heat shock protein |
| GB0417430D0 (en) * | 2004-08-05 | 2004-09-08 | Uc3 | A novel HPV vaccine comprising peptides from host cell proteins |
| US8399610B2 (en) * | 2004-08-05 | 2013-03-19 | University College Cardiff Consultants Limited | HPV vaccine comprising peptides from host cell proteins |
| JP4474264B2 (ja) | 2004-08-20 | 2010-06-02 | 生寶生物科技股▲ふん▼有限公司 | 子宮頸癌抑制の融合蛋白 |
| EP1819835B1 (en) | 2004-12-08 | 2010-08-04 | Gen-Probe Incorporated | Detection of nucleic acids from multiple types of human papillomaviruses |
| CA2594040A1 (en) | 2005-01-06 | 2006-07-13 | The Johns Hopkins University | Rna interference that blocks expression of pro-apoptotic proteins potentiates immunity induced by dna and transfected dendritic cell vaccines |
| MX2007008553A (es) * | 2005-01-14 | 2007-09-25 | Univ Michigan | Sistemas, metodos y composiciones para deteccion del virus de papiloma humano en muestras biologicas. |
| CA2595726A1 (en) * | 2005-01-26 | 2006-08-03 | The Johns Hopkins University | Anti-cancer dna vaccine employing plasmids encoding mutant oncoprotein antigen and calreticulin |
| USRE46453E1 (en) * | 2006-04-19 | 2017-06-27 | Postech Foundation | Compositions comprising HPV polypeptides and immunoenhancement peptides for the treatment and prevention of cervical cancer |
| US9085638B2 (en) | 2007-03-07 | 2015-07-21 | The Johns Hopkins University | DNA vaccine enhancement with MHC class II activators |
| HRP20140102T1 (hr) * | 2007-06-01 | 2014-03-28 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Cjepivo za prevenciju relapsa raka dojke |
| US20090117123A1 (en) * | 2007-11-02 | 2009-05-07 | National Health Research Institutes | Immunopeptides of hpv e6 and e7 proteins |
| US20120121715A1 (en) * | 2008-01-02 | 2012-05-17 | National Taiwan University | DNA Vaccine Comprising IL-6-Encoding DNA Construct and Applications Thereof |
| EP2335730A1 (en) * | 2009-12-18 | 2011-06-22 | Deutsches Krebsforschungszentrum | Fusion polypeptides and their use for prevention and treatment of cancer |
| KR101197627B1 (ko) | 2010-01-18 | 2012-11-07 | (주)레퍼런스바이오랩 | Ｈｐｖ 감염과 관련된 암의 치료용 조성물 |
| WO2011103159A2 (en) * | 2010-02-16 | 2011-08-25 | Becton, Dickinson And Company | Assay for detecting closely- related serotypes of human papillomavirus (hpv) |
| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| PL2604629T3 (pl) | 2010-08-13 | 2018-08-31 | Genexine, Inc. | Kompozycja do zapobiegania lub leczenia raka szyjki macicy ze wzmacniaczem odporności przeciwko wirusowi brodawczaka ludzkiego |
| MX360449B (es) * | 2011-10-12 | 2018-11-01 | Univ Pennsylvania | Vacunas para el virus del papiloma humano y metodos para su uso. |
| BR112014015016B1 (pt) * | 2011-12-21 | 2023-10-03 | Nykode Therapeutics ASA | Proteína homodimérica de duas cadeias de aminoácidos idênticas,cadeia de aminoácido, molécula de ácido nucléico, composição farmacêutica,célula hospedeira, método de preparação de uma proteína homodimérica, método de preparação de uma vacina e vacina |
| WO2013112549A1 (en) | 2012-01-24 | 2013-08-01 | Sanford Research/USD | Polynucleotides for treating oncogenic viral polypeptide positive tumors |
| CN103288931B (zh) * | 2012-02-24 | 2015-05-27 | 宋硕 | 人类乳头瘤病毒免疫原性多肽、其制备方法及应用 |
| AU2014248535B2 (en) | 2013-03-12 | 2017-03-30 | Inovio Pharmaceuticals, Inc. | Improved vaccines for human papilloma virus and methods for using the same |
| KR20150130284A (ko) * | 2013-03-15 | 2015-11-23 | 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 | 암 백신 및 이를 이용한 치료 방법 |
| CN107073070A (zh) * | 2014-08-15 | 2017-08-18 | 格纳西尼有限公司 | 治疗宫颈癌的方法 |
| JP5973007B2 (ja) * | 2015-01-30 | 2016-08-17 | ジェネクサイン・インコーポレーテッド | ヒトパピローマウイルス変形体及び免疫増強剤を含む子宮頸癌の予防または治療用組成物 |
| CN121159719A (zh) | 2015-04-17 | 2025-12-19 | 高山免疫科学股份有限公司 | 具有可调的亲和力的免疫调节蛋白 |
| AU2016274655B2 (en) * | 2015-06-10 | 2021-06-17 | NeoTrail Therapeutics, Inc. | HPV vaccines |
| PT3359183T (pt) * | 2015-10-06 | 2020-08-13 | Invectys | Construções de poliepítopo para utilização em imunoterapia |
| IL260030B2 (en) | 2016-01-08 | 2025-12-01 | Nykode Therapeutics ASA | Therapeutic anticancer neoepitope vaccine |
| MX2018012472A (es) | 2016-04-15 | 2019-08-12 | Alpine Immune Sciences Inc | Proteinas inmunomoduladoras variantes de ligando icos y sus usos. |
| WO2018060288A1 (en) | 2016-09-29 | 2018-04-05 | Glaxosmithkline Biologicals S.A. | Compositions and methods of treatment of persistent hpv infection |
| CN106632694B (zh) * | 2017-01-24 | 2021-02-12 | 南京益康生物医药有限公司 | 一种重组蛋白及药物组合物与应用 |
| AU2018247958A1 (en) * | 2017-04-07 | 2019-10-10 | Hookipa Biotech Gmbh | Arenavirus particles to treat solid tumors |
| EP3461497A1 (en) * | 2017-09-27 | 2019-04-03 | GlaxoSmithKline Biologicals S.A. | Viral antigens |
| JP7749319B2 (ja) | 2017-10-10 | 2025-10-06 | アルパイン イミューン サイエンシズ インコーポレイテッド | Ｃｔｌａ－４変異型免疫調節タンパク質およびそれらの使用 |
| CA3120868A1 (en) | 2018-11-30 | 2020-06-04 | Alpine Immune Sciences, Inc. | Cd86 variant immunomodulatory proteins and uses thereof |
| WO2021026335A1 (en) * | 2019-08-08 | 2021-02-11 | The Trustees Of Indiana University | Methods for identifying and treating urinary tract infections |
| JP7460094B2 (ja) | 2020-04-24 | 2024-04-02 | ジェネクシン・インコーポレイテッド | 子宮頸がんの治療方法 |
| EP4146684A2 (en) | 2020-05-08 | 2023-03-15 | Alpine Immune Sciences, Inc. | April and baff inhibitory immunomodulatory proteins with and without a t cell inhibitory protein and methods of use thereof |
| CN114478712B (zh) * | 2022-03-29 | 2022-09-23 | 深圳吉诺因生物科技有限公司 | Hpv抗原表位及其鉴定方法、应用 |
| WO2024145863A1 (en) * | 2023-01-05 | 2024-07-11 | Virogin Biotech (Shanghai) Ltd. | A NOVEL mRNA VACCINE FOR THE TREATMENT AND PREVENTION OF HPV-ASSOCIATED LESIONS AND TUMORS |
| CN117925916A (zh) * | 2024-03-11 | 2024-04-26 | 广州市金域转化医学研究院有限公司 | Hpv dna的e6基因在制备浸润性宫颈癌诊断产品中的应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010375A2 (en) * | 1997-08-22 | 1999-03-04 | Smithkline Beecham Biologicals S.A. | Vaccine against hpv |
| US6004557A (en) * | 1994-12-20 | 1999-12-21 | Csl Limited | Variants of human papillomavirus antigens |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9105383D0 (en) | 1991-03-14 | 1991-05-01 | Immunology Ltd | An immunotherapeutic for cervical cancer |
| AUPN443995A0 (en) | 1995-07-27 | 1995-08-17 | Csl Limited | Papillomavirus polyprotein |
| CN1433428A (zh) * | 2000-04-05 | 2003-07-30 | 冲击诊断公司 | 辨别人乳头瘤病毒的免疫学方法 |
| EP1553966B1 (en) * | 2002-10-03 | 2012-08-01 | Wyeth Holdings Corporation | Fusion peptides comprising the human papillomavirus e7 and e6 polypeptides and immunogenic compositions thereof |
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- 2003-10-02 JP JP2004541707A patent/JP4755422B2/ja not_active Expired - Lifetime
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- 2003-10-02 US US10/530,253 patent/US7223408B2/en not_active Expired - Lifetime
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- 2003-10-02 CA CA2499837A patent/CA2499837C/en not_active Expired - Lifetime
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2005
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2010
- 2010-08-13 JP JP2010181424A patent/JP5398665B2/ja not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6004557A (en) * | 1994-12-20 | 1999-12-21 | Csl Limited | Variants of human papillomavirus antigens |
| WO1999010375A2 (en) * | 1997-08-22 | 1999-03-04 | Smithkline Beecham Biologicals S.A. | Vaccine against hpv |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010279386A (ja) * | 2002-10-03 | 2010-12-16 | Wyeth Holdings Corp | ヒトパピローマウイルス・ポリペプチドおよび免疫原性組成物 |
Also Published As
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| US20070275003A1 (en) | 2007-11-29 |
| WO2004030636A2 (en) | 2004-04-15 |
| JP2010279386A (ja) | 2010-12-16 |
| EP1553966A4 (en) | 2008-04-16 |
| NZ556544A (en) | 2008-11-28 |
| US7223408B2 (en) | 2007-05-29 |
| KR101145190B1 (ko) | 2012-05-14 |
| ES2391451T3 (es) | 2012-11-26 |
| US7378099B2 (en) | 2008-05-27 |
| DK1553966T3 (da) | 2012-09-17 |
| US20060014926A1 (en) | 2006-01-19 |
| AU2003282731A1 (en) | 2004-04-23 |
| EP1553966A2 (en) | 2005-07-20 |
| EP1553966B1 (en) | 2012-08-01 |
| WO2004030636A3 (en) | 2005-02-24 |
| CA2499837A1 (en) | 2004-04-15 |
| JP2006501825A (ja) | 2006-01-19 |
| AU2003282731B2 (en) | 2009-01-08 |
| CN1720060A (zh) | 2006-01-11 |
| ZA200502618B (en) | 2008-10-29 |
| JP5398665B2 (ja) | 2014-01-29 |
| CN1720060B (zh) | 2011-12-14 |
| MXPA05003395A (es) | 2005-06-22 |
| CA2499837C (en) | 2013-12-03 |
| CO5700784A2 (es) | 2006-11-30 |
| PT1553966E (pt) | 2012-10-22 |
| BR0314529A (pt) | 2005-08-09 |
| KR20050053732A (ko) | 2005-06-08 |
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