JP4705716B2 - オリゴヌクレオチドの脱保護法 - Google Patents

オリゴヌクレオチドの脱保護法 Download PDF

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Publication number
JP4705716B2
JP4705716B2 JP2000597301A JP2000597301A JP4705716B2 JP 4705716 B2 JP4705716 B2 JP 4705716B2 JP 2000597301 A JP2000597301 A JP 2000597301A JP 2000597301 A JP2000597301 A JP 2000597301A JP 4705716 B2 JP4705716 B2 JP 4705716B2
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Prior art keywords
oligonucleotide
substrate
reagent
oligonucleotides
protecting group
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JP2000597301A
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English (en)
Japanese (ja)
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JP2002536381A5 (https=
JP2002536381A (ja
Inventor
アニラ・バン
ラーズ・ホームバーグ
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000597301A 1999-02-05 2000-02-05 オリゴヌクレオチドの脱保護法 Expired - Lifetime JP4705716B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11857599P 1999-02-05 1999-02-05
US60/118,575 1999-02-05
PCT/US2000/002845 WO2000046231A1 (en) 1999-02-05 2000-02-05 Method for deprotecting oligonucleotides

Publications (3)

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JP2002536381A JP2002536381A (ja) 2002-10-29
JP2002536381A5 JP2002536381A5 (https=) 2011-03-03
JP4705716B2 true JP4705716B2 (ja) 2011-06-22

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JP2000597301A Expired - Lifetime JP4705716B2 (ja) 1999-02-05 2000-02-05 オリゴヌクレオチドの脱保護法

Country Status (10)

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US (1) US6887990B1 (https=)
EP (1) EP1149109B1 (https=)
JP (1) JP4705716B2 (https=)
AT (1) ATE443071T1 (https=)
AU (1) AU767509B2 (https=)
CA (1) CA2361079C (https=)
DE (1) DE60042962D1 (https=)
DK (1) DK1149109T3 (https=)
NO (1) NO20013804L (https=)
WO (1) WO2000046231A1 (https=)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021070494A1 (ja) 2019-10-11 2021-04-15 住友化学株式会社 核酸オリゴマーの製造方法
WO2021153047A1 (ja) 2020-01-29 2021-08-05 住友化学株式会社 核酸オリゴマーの製造方法
WO2021153770A1 (en) 2020-01-29 2021-08-05 Sumitomo Chemical Company, Limited Process of preparing nucleic acid oligomer
WO2021193954A1 (ja) 2020-03-27 2021-09-30 住友化学株式会社 核酸オリゴマーの製造方法
WO2022009959A1 (ja) 2020-07-09 2022-01-13 住友化学株式会社 核酸オリゴマーの製造方法
WO2022064908A1 (ja) 2020-09-24 2022-03-31 住友化学株式会社 核酸オリゴマーの製造方法
WO2023054350A1 (ja) 2021-09-28 2023-04-06 住友化学株式会社 精製ジクロロ酢酸の製造方法
WO2023182274A1 (ja) 2022-03-23 2023-09-28 住友化学株式会社 核酸オリゴマーの製造方法
WO2024019137A1 (ja) 2022-07-22 2024-01-25 住友化学株式会社 オリゴヌクレオチドの製造方法
WO2024024873A1 (ja) 2022-07-28 2024-02-01 住友化学株式会社 チオ化溶液
WO2024053578A1 (ja) 2022-09-07 2024-03-14 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸
WO2024089953A1 (ja) 2022-10-27 2024-05-02 住友化学株式会社 オリゴヌクレオチドの製造方法
WO2024143276A1 (ja) 2022-12-26 2024-07-04 住友化学株式会社 オリゴヌクレオチドの製造方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4503828B2 (ja) 1998-02-11 2010-07-14 ユニバーシティー オブ ヒューストン 光生成試薬を用いる化学反応および生化学反応のための方法および装置
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
WO2002046205A2 (en) 2000-12-05 2002-06-13 Avecia Limited Process for the preparation of phosphorothionate oligonucleotides
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
JP2004521628A (ja) 2001-03-08 2004-07-22 アプレラ コーポレイション オリゴヌクレオチドの切断および脱保護のための試薬
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20030153741A1 (en) * 2002-02-08 2003-08-14 Marek Kwiatkowski Methods for separating oligonucleotides
US8138330B2 (en) * 2006-09-11 2012-03-20 Sigma-Aldrich Co. Llc Process for the synthesis of oligonucleotides
US8513404B2 (en) * 2008-04-24 2013-08-20 Nitto Denko Avecia, Inc. Process for the manufacture of oligonucleotides
WO2021045141A1 (ja) * 2019-09-04 2021-03-11 神戸天然物化学株式会社 オリゴヌクレオチドの脱保護方法
EP4201947A1 (en) 2021-12-22 2023-06-28 F. Hoffmann-La Roche AG Process for the backbone deprotection of oligonucleotides containing a terminal alkyl phosphonate group
CN114573652B (zh) * 2022-03-15 2025-02-07 安徽瑞拜药业有限公司 药用核酸合成工艺
US12415172B2 (en) 2022-12-22 2025-09-16 Synthego Corporation Systems and method for automated oligonucleotide synthesis

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4426517A (en) * 1981-08-24 1984-01-17 Eli Lilly And Company Process for de-cyanoethylating blocked nucleotides
US4417046A (en) * 1981-08-24 1983-11-22 Eli Lilly And Company Process for isolating oligonucleotide product from a coupling reaction mixture
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
US5362866A (en) * 1983-09-02 1994-11-08 Molecular Biosystems, Inc. Oligonucleotide polymeric support system with an oxidation cleavable link
DE3481060D1 (de) * 1983-09-02 1990-02-22 Molecular Biosystems Inc Oligonukleotid-polymer-tragsystem.
US4672110A (en) 1983-11-29 1987-06-09 Northwestern University Method of deprotecting polynucleotide trimethylethyl phosphotriesters
JPS6242997A (ja) * 1985-08-19 1987-02-24 Akira Kaji ヌクレオシドアルキルホスホネイトの製造方法
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
DE19627898A1 (de) * 1996-07-11 1998-01-15 Hoechst Ag Festphasensynthese von Oligonucleotiden
US6127533A (en) * 1997-02-14 2000-10-03 Isis Pharmaceuticals, Inc. 2'-O-aminooxy-modified oligonucleotides
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021070494A1 (ja) 2019-10-11 2021-04-15 住友化学株式会社 核酸オリゴマーの製造方法
WO2021153047A1 (ja) 2020-01-29 2021-08-05 住友化学株式会社 核酸オリゴマーの製造方法
WO2021153770A1 (en) 2020-01-29 2021-08-05 Sumitomo Chemical Company, Limited Process of preparing nucleic acid oligomer
WO2021193954A1 (ja) 2020-03-27 2021-09-30 住友化学株式会社 核酸オリゴマーの製造方法
WO2022009959A1 (ja) 2020-07-09 2022-01-13 住友化学株式会社 核酸オリゴマーの製造方法
WO2022064908A1 (ja) 2020-09-24 2022-03-31 住友化学株式会社 核酸オリゴマーの製造方法
WO2023054350A1 (ja) 2021-09-28 2023-04-06 住友化学株式会社 精製ジクロロ酢酸の製造方法
WO2023182274A1 (ja) 2022-03-23 2023-09-28 住友化学株式会社 核酸オリゴマーの製造方法
WO2024019137A1 (ja) 2022-07-22 2024-01-25 住友化学株式会社 オリゴヌクレオチドの製造方法
WO2024024873A1 (ja) 2022-07-28 2024-02-01 住友化学株式会社 チオ化溶液
WO2024053578A1 (ja) 2022-09-07 2024-03-14 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸
WO2024089953A1 (ja) 2022-10-27 2024-05-02 住友化学株式会社 オリゴヌクレオチドの製造方法
WO2024143276A1 (ja) 2022-12-26 2024-07-04 住友化学株式会社 オリゴヌクレオチドの製造方法

Also Published As

Publication number Publication date
NO20013804L (no) 2001-08-22
DE60042962D1 (de) 2009-10-29
AU2868700A (en) 2000-08-25
DK1149109T3 (da) 2010-01-11
CA2361079C (en) 2010-05-25
AU767509B2 (en) 2003-11-13
EP1149109A1 (en) 2001-10-31
WO2000046231A1 (en) 2000-08-10
CA2361079A1 (en) 2000-08-10
NO20013804D0 (no) 2001-08-03
US6887990B1 (en) 2005-05-03
JP2002536381A (ja) 2002-10-29
ATE443071T1 (de) 2009-10-15
EP1149109B1 (en) 2009-09-16

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